Towards precision medicine:advances in 5-hydroxymethylcytosine cancer biomarker discovery in liquid biopsy

作     者：Chang Zeng Emily Kunce Stroup Zhou Zhang Brian C-HChiu Wei Zhang 

作者机构：Driskill Graduate Program in Life SciencesNorthwestern University Feinberg School of MedicineChicagoIL 60611USA Department of Preventive MedicineNorthwestern University Feinberg School of Medicine680 N.Lake Shore Dr.Suite 1400ChicagoIL 60611USA Department of Public Health SciencesUniversity of ChicagoChicagoIL 60637USA The Robert H.Lurie Comprehensive Cancer CenterNorthwestern University Feinberg School of MedicineChicagoIL 60611USA Institute of Precision MedicineJining Medical UniversityJining 272067ShandongP.R.China. 

出 版 物：《Cancer Communications》 (癌症通讯（英文）)

年 卷 期：2019年第39卷第1期

页      面：113-121页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：This work was partly supported by a grant from the National Institutes of Health P30 C060553 Career Development Fund(to W.Z.) 

主　　题：Liquid biopsy Cell-free DNA Epigenetics Cancer biomarker 5-Hydroxymethylcytosine 

摘      要：Robust and clinically convenient biomarkers for cancer diagnosis,early detection,and prognosis have great potential to improve patient survival and are the key to precision *** advent of next-generation sequencing technologies enables a more sensitive and comprehensive profiling of genetic and epigenetic information in tumor-derived *** are now able to monitor the dynamics of tumorigenesis in new dimensions,such as using circulating cell-free DNA(cfDNA)and tumor DNA(ctDNA).Mutation-based assays in liquid biopsy cannot always provide consistent results across studies due partly to intra-and inter-tumoral heterogeneity as well as technical *** contrast,epigenetic analysis of patient-derived cfDNA is a promising alternative,especially for early detection and disease surveillance,because epigenetic modifications are tissue-specific and reflect the dynamic process of cancer ***,cfDNA-based epigenetic assays are emerging to be a highly sensitive,minimally invasive tool for cancer diagnosis and prognosis with great potential in future precise care of cancer *** major obstacle for applying epigenetic analysis of cfDNA,however,has been the lack of enabling techniques with high sensitivity and technical *** this review,we summarized the advances in epigenome-wide profiling of 5-hydroxymethyl-cytosine(5hmC)in cfDNA,focusing on the detection approaches and potential role as biomarkers in different cancer types.