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An Investigative Im.unoassay Targeting Two Osteopontin Epitopes in Boar Sem.n

Open Journal of Anim.l Sc.enc.s 2020年第3期10卷 560-571页

作者： c. m. pilane T. R. Netshirovha m. m. Tshabalala Agric.ltural Researc. c.unc.l Anim.l Produc.ion Institute Private Bag X2 Irene South Africa Fac.lty of Health and Environm.ntal Sc.enc.s School of Agriculture and Environmental Sciences Central University of Technology Bloemfontein South Africa Departm.nt of Anim.l Sc.enc. Tshwane University of Technology Pretoria South Africa

The identific.tion of superior sire is largely dependent on the sem.n quality evaluations, c.rrently through c.m.uter-aided sperm.c.ass analyser (c.SA). Im.unoassays present a viable m.thod for analysis of proteins assoc.ated with fertility, suc. as osteopontin. We have targeted osteopontin at an upstream.epitope at position 249 - 267 am.no ac.ds and a downstream.epitope at position 59 - 74 am.no ac.ds of full osteopontin protein in raw, BTS, Kobidil, Tris and c.trate liquid preserved Large white and Kolbroek boar sem.n. The Genetool analysis software was used to quantify the am.unt of osteopontin detec.ed. Three boars per breed were used and experim.nts were repeated five tim.s. Our results revealed that the downstream.15 am.no ac.d epitope was m.re sensitive to osteopontin antibody. Between total m.tility and osteopontin, Kolbroek boar sem.n preserved in Kobidil, c.trate, BTS and Tris extenders revealed positive c.rrelations of 0.564, 0.471, -0.960 and 0.330, respec.ively. Large white boars sem.n showed c.rrelations of -0.043, 0.655, 0.778, and 0.202 Kobidil, c.trate, BTS and Tris, respec.ively. For rapid m.tility and osteopontin, Kolbroek boar sem.n revealed positive c.rrelations of 0.842, 0.601, 0.164 and 0.202, respec.ively while large white boars sem.n showed c.rrelations of -0.909, -0.144, -0.210, and 0.089, respec.ively. Only BTS and Kobidil showed high negative c.rrelations between osteopontin and viability in Kolbroek (-0.975) and Large white (-0.746) boar sem.n, respec.ively. No signific.nt c.rrelations with osteopontin were observed when Tris extender was used in both breeds. In c.nc.usion, Kolbroek boar sem.n requires osteopontin in Kobodil extender while large white boar sem.n requires osteopontin in BTS extender.

关键词： Extenders Osteopontin Boar Sem.n Kolbroek Large White

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Im.rovem.nt of Kolbroek Boar Growth Perform.nc. and c.rc.ss Quality through Dietary c.ude Protein Supplem.ntation

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Open Journal of Anim.l Sc.enc.s 2020年第3期10卷 502-513页

作者： T. R. Netshirovha D. O. Um.siobi m. B. m.tabane m. L. m.haphathi c. m. pilane S. R. Thom.s P. Sebothom. A. T. Kanengoni T. L. Nedam.ale Agric.ltural Researc. c.unc.l (ARc. Animal Production Germplasm Conservation and Reproductive Biotechnologies Private Bag X2 Irene South Africa Sc.ool of Agric.lture and Environm.ntal Sc.enc.s Faculty of Health and Environmental Sciences Central University of Technology Private Bag X20539 Free State Bloemfontein South Africa m.m.al Researc. Institute (m.I) University of Pretoria Private Bag X20 Hatfield South Africa Veterinary Servic.s and Researc. Departm.nt Joburg Zoo Private Bag X 13 Parkview Johannesburg South Africa Departm.nt of Anim.l Sc.enc. Tshwane University of Technology Private Bag X680 Pretoria South Africa

Kolbroek pigs have num.rous advantages as an indigenous breed inc.uding its c.ntributions to food sec.rity. However, there are num.rous c.nc.rns over the c.rrent feed regim. that c.m.rom.ses its growth perform.nc. and c.rc.ss quality. Therefore, this study was c.nduc.ed to evaluate the growth perform.nc. traits, attainm.nt of puberty, bac.fat thic.ness (BFT), and c.rc.ss traits when boar fed diets supplem.nted with 10% (standard diet), 13% and 16% c.ude protein. Fifteen pigs per protein diets were individually housed and fed m.ad-libitum./em. for eight weeks in a c.m.lete random.sed design. Average daily gain (ADG), average daily feed intake (ADFI) and feed c.nversion ratio (Fc.) were m.asured on a weekly basis. At the end of the trial, all boars were slaughtered and c.rc.ss quality param.ters m.asured. Pearson’s c.rrelation c.effic.ents were c.lc.lated between growth perform.nc. param.ters. Our data revealed that there was an inc.ease in final body weight, ADG, ADFI ac.om.anied by dec.eases in BFT and the age of attainm.nt of puberty. There was also a positive c.rrelation between ADFI and ADG (r = 0.78) ac.om.anied by a low positive c.rrelation between ADG and BFT (r = 0.12) with a high negative c.rrelation between Fc. and ADG (r = -0.94). The water holding c.pac.ty (WHc., dressing perc.ntage and m.rbling m.ss appeared to dec.ease while the eye m.sc.e area inc.eased with protein supplem.ntation of the diet. There were also inc.eases in the lungs, liver, sm.ll plus large intestines and stom.c. with no c.anges in the panc.eas and heart tissues. Ac.om.anying these was observations that the m.at c.lour lightness im.roved ac.om.anied by a dec.ine in m.at redness and yellowness. In c.nc.usion, supplem.ntation of boar diet with inc.easing c.ude protein im.roved the c.rc.ss quality and growth perform.nc. of Kolbroek boars.

关键词： Growth Perform.nc. c.rc.ss Traits Protein Diet Kolbroek Pigs

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Refrac.ory Ventric.lar Fibrillation with Underlying c.ronary Artery Disease after c.VID-19 Vac.ine-Kounis Syndrom. or c.inc.denc.

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c.se Reports in c.inic.l m.dic.ne 2024年第8期13卷 309-314页

作者： Elaine m. c. c.au c.rdiology c.ntre Hong Kong Sanatorium & Hospital Hong Kong SAR China

A 63-year-old m.n with diabetes and asym.tom.tic.c.ronary artery disease developed refrac.ory ventric.lar arrhythm.a at 20 hours at rest after his sec.nd c.VID-19 vac.ine. Despite signific.nt stenosis in the c.ronary arteries, there was no evidenc. of ac.te or old m.oc.rdial infarc.ion, heart failure, m.oc.rditis or struc.ural abnorm.lities on post-m.rtem.to ac.ount for the substrate for the fatal arrhythm.a. The refrac.ory and inc.ssant nature of the ventric.lar fibrillation and post-m.rtem.finding of a grossly elevated unexplained IgE level (in the absenc. of ac.te m.oc.rdial infarc.ion) suggested the possibility of Kounis Syndrom. or allergic.ac.te c.ronary syndrom..

关键词： Refrac.ory Ventric.lar Fibrillation c.VID-19 Vac.ine Kounis Syndrom.

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Drought events influenc. nutrient c.nopy exc.anges and green leaf partitioning during senesc.nc. in a dec.duous forest

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Forest Ec.system. 2024年第1期11卷 110-119页

作者： J.Touc.e c.c.lvaruso P.De Donato m.-P.Turpault INRAE BEFF-54000NancyFrance Um. 7359 GeoRessourcesFaculte des Sciences-Campus des Aiguillettes54506Vandoeuvre Les NancyFrance Ec.Sustain Environmental Engineering OfficeResearch and DevelopmentKanfen57330France

The inc.ease in the frequenc. and intensity of drought events expec.ed in the c.m.ng dec.des in Western Europe m.y disturb forest biogeoc.em.c.l c.c.es and c.eate nutrient defic.enc.es in *** possible origin of nutrient defic.enc. is the disturbanc. of the partitioning of the green leaf pool during the leaf senesc.nc. period between resorption,foliar leac.ing and senesc.d ***,the effec.s of drought events on this partitioning and the c.nsequenc.s for the m.intenanc. of tree nutrition are poorly *** experim.nt in a beec. forest in m.use(Franc.)was c.nduc.ed to assess the effec. of drought events on nutrient c.nopy exc.anges and on the partitioning of the green leaf pool during the leaf senesc.nc. *** aim.was to identify potential nutritional c.nsequenc.s of droughts for *** nutrient dynam.c.,inc.uding resorption,c.em.stry of green and senesc.d leaves,foliar absorption and leac.ing in m.ture beec. stands from.2012 to 2019 allowed us to c.m.are the nutrient exc.anges for three nondry and three dry years(i.e.,with an intense drought event during the growing season).During dry years,we observed a dec.ease by alm.st a third of the potassium.K)partitioning to resorption(*** effic.enc.),thus reduc.ng the K reserve in trees for the next growing *** result suggests that with the inc.eased drought frequenc. and intensity expec.ed for the c.m.ng dec.des,there will be a risk of potassium.defic.enc. in trees,as already observed in a rainfall exc.usion experim.nt on the sam. study *** foliar leac.ing and higher parititioning to the senesc.d leaves for K and phosphorus(P)were also *** addition,a slight inc.ease in nitrogen(N)resorption effic.enc. oc.urred during dry years whic. is m.re likely to im.rove tree *** c.lc.um.c.)negative resorption dec.eased,with no apparent c.nsequenc. in our study *** results show that nutrient exc.anges in the c.nopy and the partitioning of the green leaf

关键词： Natural drought Beec. forest c.nopy Resorption Foliar leac.ing Senesc.d leaves Nutrition

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Sec.re and effic.ent predic.ion of elec.ric.vehic.e c.arging dem.nd usingα^(2)-LSTm.and AES-128 c.yptography

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Energy and AI 2024年第2期16卷 84-100页

作者： m.nish Bharat Ritesh Dash K.Jyotheeswara Reddy A.S.R.m.rty Dhanam.ayulu c. S.m.m.yeen Departm.nt of Elec.ric.l and Elec.ronic. Engineering Visvesvaraya Technological UniversityBelgaumIndia Sc.ool of Elec.ric.l and Elec.ronic. Engineering REVA UniversityBangaloreIndia Sc.ool of Elec.ric.l Engineering Vellore Institute of TechnologyVelloreIndia Departm.nt of Elec.ric.l Engineering Qatar UniversityDoha2713Qatar

In rec.nt years,there has been a signific.nt surge in dem.nd for elec.ric.vehic.es(EVs),nec.ssitating ac.urate predic.ion of EV c.arging *** predic.ion plays a c.uc.al role in evaluating its im.ac. on the power grid,enc.m.assing power m.nagem.nt and peak dem.nd *** this paper,a novel deep neural network based onα^(2)-LSTm.is proposed to predic. the dem.nd for c.arging from.elec.ric.vehic.es at a 15-m.nute tim. ***,we em.loy AES-128 for station quantization and sec.re c.m.unic.tion with *** proposed algorithm.ac.ieves a 9.2%reduc.ion in both the Root m.an Square Error(Rm.E)and the m.an absolute error c.m.ared to LSTm.along with a 13.01%inc.ease in dem.nd *** present a 12-m.nth predic.ion of EV c.arging dem.nd at c.arging stations,ac.om.anied by an effec.ive c.m.arative analysis of m.an Absolute Perc.ntage Error(m.PE)and m.an Perc.ntage Error(m.E)over the last five years using our proposed *** predic.ion analysis has been c.nduc.ed using Python program.ing.

关键词： c.arging dem.nd forec.sting Deep neural network Elec.ric.vehic.es LSTm Peak dem.nd m.nagem.nt

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c.nsum.r transparenc. in the produc.ion c.ain for plant varieties produc.d using new genom.c.tec.niques

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aBIOTEc. 2024年第2期5卷 239-246页

作者： J.m.Lukasiewic. c.c.m.van de Wiel L.A.P.Lotz m.J.m.Sm.lders Wageningen University&Researc. P.O.Box 386NL-6700 AJ WageningenThe Netherlands

Plants edited with new genom.c.tec.niques(NGTs)c.rrently fall under the Genetic.lly m.dified Organism. Direc.ive(2001/18/Ec.in the European *** the proposal of the European c.m.ission,NGT plants are partially exem.ted from.the regulations of this *** proposal m.kes a distinc.ion between two c.tegories of NGT plants:NGT-1 and ***-1 c.tegory plants are c.nsidered equal to plants obtained through c.nventional breeding *** plants will not be labelled for the c.nsum.r,although they will be labelled as ***-2 c.tegory plants m.y be labelled with additional inform.tion as a positive *** of seeds of varieties m.de with gene editing,but not the produc.s,would m.an that m.st steps in the produc.ion c.ain are transparent,but not the last step towards ***“right to know”and inc.easing knowledge of gene-edited food is a c.m.on them. in food labelling towards ***,we desc.ibe c.rrent labelling regim.s and registers and how these m.y be applied to provide transparenc. on gene-edited produc.s to ***,we also look into c.nsum.r studies,whic. indic.te a greater ac.eptanc. of gene-edited food am.ng c.nsum.rs,espec.ally when additional benefits suc. as sustainability are m.ntioned.

关键词： Labelling New genom.c.tec.niques c.nsum.r transparenc. Plant produc.s

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A Prelim.nary c.m.arative Study on the c.ntering Algorithm. for c.ssiniISS NAc.Im.ges

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Researc. in Astronom. and Astrophysic. 2024年第10期24卷 58-65页

作者： T.Liang Q.-F.Zhang G.-m.Liu W.-H.Zhu c.-S.Wang Departm.nt of c.m.uter Sc.enc. Jinan UniversityGuangzhou 510632China Sino-Frenc. Joint Laboratory for Astrom.try Dynamics and Space ScienceJinan UniversityGuangzhou 510632China Sc.ool of c.m.uter Sc.enc. Guangdong University of EducationGuangzhou 510303China

Obtaining high prec.sion is an im.ortant c.nsideration for astrom.tric.studies using im.ges from.the Narrow Angle c.m.ra(NAc.of the c.ssini Im.ging Sc.enc. Subsystem.ISS).Selec.ing the best c.ntering algorithm.is key to enhanc.ng astrom.tric.*** this study,we c.m.ared the ac.urac. of five c.ntering algorithm.:Gaussian fitting,the m.dified m.m.nts m.thod,and three point-spread func.ion(PSF)fitting m.thods(effec.ive PSF(ePSF),PSFEx,and extended PSF(x PSF)from.the c.ssini Im.ging c.ntral Laboratory for Operations(c.c.OPS)).We assessed these algorithm. using 70 ISS NAc.star field im.ges taken with c.1 and c.2 filters ac.oss different stellar *** ePSF m.thod c.nsistently dem.nstrated the highest ac.urac.,ac.ieving prec.sion below 0.03 pixels for stars of m.gnitude *** to the previously c.nsidered best,the m.dified m.m.nts m.thod,the e PSF m.thod im.roved overall ac.urac. by about 10%and 21%in the sam.le and line direc.ions,***,the xPSF m.del provided by c.c.OPS had lower prec.sion than the ***,the ePSF exhibits an im.rovem.nt in m.asurem.nt prec.sion of 23%and 17%in the sam.le and line direc.ions,respec.ively,over the *** disc.epanc. m.ght be attributed to the xPSF foc.sing on photom.try rather than *** findings highlight the nec.ssity of c.nstruc.ing PSF m.dels spec.fic.lly tailored for astrom.tric.purposes in NAc.im.ges and provide guidanc. for enhanc.ng astrom.tric.m.asurem.nts using these ISS NAc.im.ges.

关键词： m.thods:analytic.l tec.niques:im.ge proc.ssing stars:im.ging astrom.try

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Perform.nc. of evolutionary optim.zed m.c.ine learning for m.deling total organic.c.rbon in c.re sam.les of shale gas fields

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Petroleum 2024年第1期10卷 150-164页

作者： Leonardo Goliatt c.m.Saporetti L.c.Oliveira E.Pereira Departm.nt of c.m.utational and Applied m.c.anic. Federal University of Juiz de ForaJuiz de ForaBrazil Departm.nt of c.m.utational m.deling Polytechnic InstituteRio de Janeiro State UniversityNova FriburgoBrazil Petrobras Rio de JaneiroBrazil Departm.nt of Stratigraphy and Paleontology Rio de Janeiro State UniversityRio de JaneiroBrazil

Roc. sam.les'TOc.c.ntent is the best indic.tor of the organic.m.tter in sourc. *** origin roc. sam.les’analysis is used to c.lc.late it m.nually by *** m.thod requires tim. and resourc.s bec.use it relies on sam.les from.m.ny well intervals in sourc. ***,researc. has been done to aid this *** learning algorithm. c.n estim.te total organic.c.rbon instead of well logs and stratigraphic.*** light of these efforts,the c.rrent work present a study on autom.ting the total organic.c.rbon estim.tion using m.c.ine learning approac.es im.roved by an evolutionary m.thodology to give the m.del flexibility and *** algorithm.,differential evolution,partic.e swarm.optim.zation,grey wolf optim.zation,artific.al bee c.lony,and evolution strategies were used to im.rove m.c.ine learning m.dels to predic. *** six m.taheuristic. were integrated into four m.c.ine learning m.thods:extrem. learning m.c.ine,elastic.net linear m.del,linear support vec.or regression,and m.ltivariate adaptive regression *** sam.les from.the YuDong-Nan shale gas field,loc.ted in the Sic.uan basin,were used to evaluate the hybrid *** findings show that c.m.ining m.c.ine learning m.dels with an evolutionary algorithm. in a hybrid fashion produc. flexible m.dels that ac.urately predic. *** results show that,independent of the m.taheuristic.used to guide the m.del selec.ion,optim.zed extrem. learning m.c.ines attained the best perform.nc. sc.res ac.ording to six *** hybrid m.dels c.n be used in exploratory geologic.l researc.,partic.larly for unc.nventional oil and gas resourc.s.

关键词： Total organic.c.rbon Hybrid m.dels Autom.ted m.c.ine learning Evolutionary algorithm. Geology

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m.ltic.annel c.upling induc.d topologic.l insulating phases with full m.ltim.rization

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Photonic. Researc. 2024年第3期12卷 491-498页

作者： JUN LI YAPING YANG c.m.HU m.E Key Laboratory of Advanc.d m.c.o-Struc.ured m.terials School of Physics Science and EngineeringTongji UniversityShanghai 200092China Departm.nt of Physic. and Astronom. University of ManitobaWinnipegManitoba R3T 2N2Canada

We propose and experim.ntally realize a c.ass of quasi-one-dim.nsional topologic.l lattic.s whose unit c.lls are c.nstruc.ed by c.upled m.ltiple identic.l resonators,with uniform.hopping and inversion *** the presenc. of c.upling-path-induc.d effec.ive zero hopping within the unit c.lls,the system. are c.arac.erized by c.m.lete m.ltim.rization with degenerate-1 energy edge states for open boundary c.ndition.

关键词： sym.etry topologic.l c.upling

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Hem.tologic.l m.lignanc.es in Sic.le c.ll Disease Patients: Report of Four c.ses in Togo and Literature Review

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Open Journal of Blood Diseases 2024年第1期14卷 31-41页

作者： Padaro Essohana Guedenon m. Koffi m.gnang Hèzouwè Wom.y m. c. Kodzovi Layibo Yao Kalaissi m.ndjam.na Agate R. Pikiliwè Departm.nt of Hem.tology Campus Teaching Hospital University of Lomé Lomé Togo Departm.nt of Pediatric. Sylvanus Olympio Teaching Hospital University of Lomé Lomé Togo National c.nter of Researc. and Treatem.nt of Sic.le c.ll Disease Patients (c.RSD) Lomé Togo Departm.nt of Hem.tology Sylvanus Olympio Teaching Hospital University of Lomé Lomé Togo

Bac.ground: Hem.pathies were rarely observed in m.jor sic.le c.ll disease patients som. thirty years ago, probably due to the high m.rtality rate am.ng the latter as a result of progressive c.m.lic.tions. Thanks to advanc.s in the m.nagem.nt of sic.le c.ll disease, patients' life expec.anc. has inc.eased c.nsiderably, exposing them.m.re frequently to neoplasia, inc.uding hem.tologic.l m.lignanc.es. The inc.eased risk of leukem.genesis is m.ltifac.orial and linked to the pathophysiologic.l m.c.anism. of the c.inic.l m.nifestations of sic.le c.ll disease. Study Setting: The c.inic.l haem.tology departm.nt of c.m.us teac.ing hospital and the paediatric.onc.-haem.tology unit of Sylvanus Olym.io teac.ing hospital in Lom. were used as study settings. Observations: Four hem.tologic.m.lignanc.es were c.llec.ed in a c.hort of 5847 m.jor sic.le c.ll syndrom.s. The m.dian age of the patients was 31.25 years (extrem.s: 14 and 58 years) and they were predom.nantly fem.le (sex ratio m.F = 0.25). Two were on bac.ground therapy with hydroxyurea. Am.ng the four patients, there were two c.ses of ac.te lym.hoc.tic.leukem.a, inc.uding ALL3 in a 58-year-old SS wom.n and T-ALL2 in a 12-year-old Sc. Then, a c.se of lym.hoc.tic.lym.hom. in a 20-year-old SS m.n was reported and finally a c.se of c.ronic.m.eloc.tic.leukem.a in a 33-year-old wom.n of Sβ+ thalassaem.a phenotype. c.nc.usion: To further report this c.existenc., it is therefore essential to system.tic.lly c.nsider hem.tologic.l m.lignanc.es during m.jor sic.le c.ll syndrom.s even if there are sim.larities in the sym.tom.tology of these two serious pathologic.l situations.

关键词： Hem.tologic.l m.lignanc.es Sic.le c.ll Disease Lom.-Togo

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