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Thermodynamics of ABc transporters

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Protein & cell 2016年第1期7卷 17-27页

作者： Xuejun c. zhang Lei Han Yan Zhao National Laboratory of Macromolecules National Center of Protein Science-Beijing Institute of Biophysics Chinese Academyof Sciences Beijing 100101 China

ABc transporters form the largest of all transporter families, and their structural study has made tremen- dous progress over recent years. However, despite such advances, the precise mechanisms that determine the energy-coupling between ATP hydrolysis and the con- formational changes following substrate binding remain to be elucidated. Here, we present our thermodynamic analysis for both ABc importers and exporters, and introduce the two new concepts of differential-binding energy and elastic conformational energy into the dis- cussion. We hope that the structural analysis of ABc transporters will henceforth take thermodynamic aspects of transport mechanisms into account as well.

关键词： ABc transporters differential-bindingenergy energy-coupling elastic conformational energy

Diffusion behavior at void tip and its contributions to void shrinkage during solid-state bonding

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Journal of Materials Science & Technology 2018年第8期34卷 1449-1454页

作者： c.zhang M.Q.Li H.Li School of Materials Science and Engineering Northwestern Polytechnical University State Key Laboratory of Solidification Processing Northwestern Polytechnical University Department of Mechanical Engineering University de Lyon

Solid-state diffusion bonding is an advanced joining technique, which has been widely used to join similar or dissimilar materials. Generally, it is easy to observe the diffusion behavior during dissimilar bonding, but for similar bonding the diffusion behavior has yet been observed via experiments. In this study, the diffusion behavior at void tip was firstly observed during similar bonding of stainless steel. Scanning electron microscopy with energy dispersive spectroscopy was used to examine the interface charac- teristic and diffusion behavior. The results showed that a diffusion region was discovered at void tip. Element concentrations of diffusion region were more than those of void region, but less than those of bonded region. This behavior indicated that the diffusion was ongoing at void tip, but the perfect bond has yet formed. The diffusion region was attributed to the interface diffusion from adjacent region to void tip due to the stress gradient along bonding interface. The mass accumulation at void tip transformed the sharp void tip into smooth one at the beginning of void shrinkage, and then resulted in shorter voids.

关键词： Stainless steel Micro-void morphology Solid-state bonding Void tip Element diffusion

Amplitude analysis of the decays D^(0)→π^(+)π^(−)π^(+)π^(−)and D^(0)→π^(+)π^(−)π^(0)π^(0)

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chinese Physics c 2024年第8期48卷 6-33页

作者： M.Ablikim M.N.Achasov P.Adlarson O.Afedulidis X.c.Ai R.Aliberti A.Amoroso Q.An Y.Bai O.Bakina I.Balossino Y.Ban H.-R.Bao V.Batozskaya K.Begzsuren N.Berger M.Berlowski M.Bertani D.Bettoni F.Bianchi E.Bianco A.Bortone I.Boyko R.A.Briere A.Brueggemann H.cai X.cai A.calcaterra G.F.cao N.cao S.A.cetin J.F.chang W.L.chang G.R.che G.chelkov c.chen c.H.chen chao chen G.chen H.S.chen M.L.chen S.J.chen S.L.chen S.M.chen T.chen X.R.chen X.T.chen Y.B.chen Y.Q.chen Z.J.chen Z.Y.chen S.K.choi X.chu G.cibinetto F.cossio J.J.cui H.L.Dai J.P.Dai A.Dbeyssi R.E.de Boer D.Dedovich c.Q.Deng Z.Y.Deng A.Denig I.Denysenko M.Destefanis F.De Mori B.Fang S.S.Fang W.X.Fang Y.Fang Y.Q.Fang R.Farinelli L.Fava F.Feldbauer G.Felici c.Q.Feng J.H.Feng Y.T.Feng K.Fischer M.Fritsch c.D.Fu J.L.Fu Y.W.Fu H.Gao Y.N.Gao Yang Gao S.Garbolino I.Garzia P.T.Ge Z.W.Ge c.Geng E.M.Gersabeck B.Ding X.X.Ding Y.Ding Y.Ding J.Dong L.Y.Dong M.Y.Dong X.Dong M.c.Du S.X.Du Z.H.Duan P.Egorov Y.H.Fan J.Fang JA.Gilman K.Goetzen L.Gong W.X.Gong W.Gradl S.Gramigna M.Greco M.H.Gu Y.T.Gu c.Y.Guan Z.L.Guan A.Q.Guo L.B.Guo M.J.Guo R.P.Guo Y.P.Guo A.Guskov J.Gutierrez K.L.Han T.T.Han X.Q.Hao F.A.Harris K.K.He K.L.He F.H.Heinsius c.H.Heinz Y.K.Heng c.Herold T.Holtmann P.c.Hong G.Y.Hou X.T.Hou Y.R.Hou Z.L.Hou B.Y.Hu H.M.Hu J.F.Hu T.Hu Y.Hu G.S.Huang K.X.Huang L.Q.Huang X.T.Huang Y.P.Huang T.Hussain F.H\"olzken N.H\"usken N.in der Wiesche M.Irshad J.Jackson S.Janchiv J.H.Jeong Q.Ji Q.P.Ji W.Ji X.B.Ji X.L.Ji Y.Y.Ji X.Q.Jia Z.K.Jia D.Jiang H.B.Jiang P.c.Jiang S.S.Jiang T.J.Jiang X.S.Jiang Y.Jiang J.B.Jiao J.K.Jiao Z.Jiao S.Jin Y.Jin M.Q.Jing X.M.Jing T.Johansson S.Kabana N.Kalantar-Nayestanaki X.L.Kang X.S.Kang M.Kavatsyuk B.c.Ke V.Khachatryan A.Khoukaz R.Kiuchi O.B.Kolcu B.Kopf M.Kuessner X.Kui A.Kupsc W.K\"uhn J.J.Lane P.Larin L.Lavezzi T.T.Lei Z.H.Lei H.Leithoff M.Lellmann T.Lenz c.Li c.Li c.H.Li cheng Li D.M.Li F.Li G.Li H.Li H.B.Li H.J.Li H.N.Li Hui Li J.R.Li J.S.Li K.Li L.J.Li L.K.Li Lei Li M.H.Li P.R.Li Q.M.Li Q.X.Li R.Li S.X.Li T.Li W.D.Li W.G.Li X.Li X.H.Li X.L.Li X.Y.Li Y Institute of High Energy Physics Chinese Academy of SciencesBeijing 100049China Beihang University Beijing 100191China Bochum Ruhr-University D-44780 BochumGermany Budker Institute of Nuclear Physics SB RAS(BINP) Novosibirsk 630090Russia carnegie Mellon University PittsburghPennsylvania 15213USA central china Normal University Wuhan 430079China central South University Changsha 410083China china center of Advanced Science and Technology Beijing 100190China china University of Geosciences Wuhan 430074China chung-Ang University Seoul06974Republic of Korea cOMSATS University Islamabad Lahore CampusDefence RoadOff Raiwind Road54000 LahorePakistan Fudan University Shanghai 200433China GSI Helmholtzcentre for Heavy Ion Research GmbH D-64291 DarmstadtGermany Guangxi Normal University Guilin 541004China Guangxi University Nanning 530004China Hangzhou Normal University Hangzhou 310036China Hebei University Baoding 071002China Helmholtz Institute Mainz Staudinger Weg 18D-55099 MainzGermany Henan Normal University Xinxiang 453007China Henan University Kaifeng 475004China Henan University of Science and Technology Luoyang 471003China Henan University of Technology Zhengzhou 450001China Huangshan college Huangshan 245000China Hunan Normal University Changsha 410081China Hunan University Changsha 410082China Indian Institute of Technology Madras Chennai 600036India Indiana University BloomingtonIndiana 47405USA INFN Laboratori Nazionali di Frascati INFN Laboratori Nazionali di FrascatiI-00044FrascatiItaly INFN Laboratori Nazionali di Frascati INFN Sezione di PerugiaI-06100PerugiaItaly INFN Laboratori Nazionali di Frascati University of PerugiaI-06100PerugiaItaly INFN Sezione di Ferrara INFN Sezione di FerraraI-44122FerraraItaly INFN Sezione di Ferrara University of FerraraI-44122FerraraItaly Inner Mongolia University Hohhot 010021China Institute of Modern Physics Lanzhou 730000China Institute of Physics and Technology Peace Avenue 54BUlaanbaatar 13330Mongolia Instituto de A

Using e^(+)e^(−)annihilation data corresponding to an integrated luminosity of 2.93 fb^(−1)taken at the center-of-mass energy√s=3.773 GeV with the BESIII detector,a joint amplitude analysis is performed on the decays D^(0)→π^(+)π^(−)π^(+)π^(−)and D^(0)→π^(+)π^(−)π^(0)π^(0)(non-η).The fit fractions of individual components are obtained,and large interferences among the dominant components of the decays D^(0)→a_(1)(1260)π,D^(0)→π(1300)π,D^(0)→ρ(770)ρ(770),and D^(0)→2(ππ)_(S)are observed in both *** the obtained amplitude model,the cP-even fractions of D^(0)→π^(+)π^(−)π^(+)π^(−)and D^(0)→π^(+)π^(−)π^(0)π^(0)(non-η)are determined to be(75.2±1.1_(stat).±1.5_(syst.))%and(68.9±1.5_(stat).±2.4_(syst.))%,*** branching fractions of D^(0)→π^(+)π^(−)π^(+)π^(−)and D^(0)→π^(+)π^(−)π^(0)π^(0)(non-η)are measured to be(0.688±0.010_(stat.)±0.010_(syst.))%and(0.951±0.025_(stat.)±0.021_(syst.))%,*** amplitude analysis provides an important model for the binning strategy in measuring the strong phase parameters of D^(0)→4πwhen used to determine the cKM angleγ(ϕ_(3))via the B^(−)→DK^(−)decay.

关键词： BESIII D^(0)meson decays amplitude analysis cP-even fraction

crystal structures of NAc domains of human nascent polypeptide-associated complex (NAc) and its αNAc subunit

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Protein & cell 2010年第4期1卷 406-416页

作者： Lanfeng Wang Wenchi zhang Lu Wang Xuejun c.zhang Xuemei Li Zihe Rao National Laboratory of Biomacromolecules Institute of BiophysicsChinese Academy of Sciences15 Datun RoadBeijing 100101China Structure Biology Laboratory Tsinghua UniversityBeijing 100084China Tianjin Key Laboratory of Protein Science College of Life SciencesNankai UniversityTianjin 300071China

Nascent polypeptide associated complex(NAc)and its two isolated subunits,αNAc and βNAc,play important roles in nascent peptide *** determined a 1.9Åresolution crystal structure of the interaction core of NAc heterodimer and a 2.4Åresolution crystal structure ofαNAc NAc domain *** structures provide detailed information of NAc heterodimerization and αNAc *** found that the NAc domains of αNAc and βNAc share very similar folding despite of their relative low identity of amino acid ***,different electric charge distributions of the two subunits at the NAc interface provide an explanation to the observation that the heterodimer of NAc complex is more stable than the single subunit *** addition,we successfully built a βNAc NAc domain homodimer model based on homologous modeling,suggesting that NAc domain dimerization is a general property of the NAc *** 3D structures allow further studies on structurefunction relationship of NAc.

关键词： nascent polypeptide-associated complex αNAc homodimer βNAc crystal structure

Why is dimerization essential for class-c GPcR function？ New insights from mGluR1 crystal structure analysis

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Protein & cell 2014年第7期5卷 492-495页

作者： Xuejun c. zhang Jianfeng Liu Daohua Jiang National Laboratory of Macromolecules National Center of Protein Science--Beijing Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China School of Life Science and Technology Huazhong University of Science and Technology Wuhan 430074 China

G-protein coupled receptors （GPcRs） play an essential role in eukaryotic cells signaling. According to phylogenetic analysis, most GPcRs belong to one of four classes, i.e. A, B, c, and Frizzled （Lagerstrom and Schioth, 2008）. The class-c GPcR family contains metabotropic glutamate receptors （mGluR）, y-aminobutyric acid B receptors （GABAB receptor or GBR）, several taste-sensing receptors （e.g.

关键词： Linker Peptide Inactive conformation Extracellular Side Large conformational change cytosolic Side

Studies on Diastereoselective Intramolecular meta-cycloaddition ofArene to Olefin (Part I)

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chinese chemical Letters 1998年第4期9卷 131-134页

作者： Shen, XM Guo, Xc Sun, LD zhang, c Beijing Normal Univ Dept Chem Beijing 100875 Peoples R China

The photoinduced intramolecular meta-cycloaddition of bichromophores 1a-1d gave diastereoselectively two types of adducts, 2a-2d and 3a-3d. respectively. The observed stereochemistry of cycloaddition was rationalized ... 详细信息

关键词： arene olefin meta-cycloaddition diastereoselectivity

In-plane crushing behavior and energy absorption design of composite honeycombs

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Acta Mechanica Sinica 2018年第6期34卷 1108-1123页

作者： H.X.Wu Y.Liu X.c.zhang School of civil Engineering Northeast Forestry UniversityHarbin 150040China Institute of Mechanics Beijing Jiaotong UniversityBeijing 100044China Department of Mechanical Engineering North China Electric Power UniversityBaoding 071003China

Theoretical analysis and numerical simulation methods were used to study the in-plane crushing behavior of single-cell structures and regular and composite ***,hexagonal,and circular honeycombs were selected as honeycomb layers to establish composite honeycomb models in the form of composite structures and realize the complementary advantages of honeycombs with type Ⅰ and type Ⅱ *** effects of honeycomb layer arrangement,plastic collapse strength,relative density,and crushing velocity on the deformation mode,plateau stress,load uniformity,and energy absorption performance of the composite honeycombs were mainly considered.A semi-empirical formula for plateau stress and energy absorption rate per unit mass for the composite honeycombs was *** results showed that the arrangement mode of honeycomb layers is an important factor that affects their mechanical *** selecting the arrangement of honeycomb layers and the proportion of honeycomb layers with different structures in a composite honeycomb can effectively improve its load uniformity and control the magnitude of plateau stress and energy absorption capacity.

关键词： composite honeycombs Dynamic crushing Load uniformity Energy absorption Finite element analysis

Binary doping of nitrogen and phosphorus into porous carbon: A novel di-functional material for enhancing cO_(2) capture and super-capacitance

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Journal of Materials Science & Technology 2022年第4期99卷 73-81页

作者： Yuan Wang Jianfei Xiao Hanzhi Wang Tian c.zhang Shaojun Yuan Low-carbon Technology&chemical Reaction Engineering Lab College of Chemical EngineeringSichuan UniversityChengdu 610065China civil&Environmental Engineering Department University of Nebraska-LincolnOmahaNE 68182-0178USA

Designing of hetero-atomic doped carbon-based systems through pyrolysis of abundant element organic precursors is a novel approach to construct rational porous carbon ***,a highly-cross-linked triazine polymer is employed to fabricate N,P co-doped porous carbon(A-TDP-12)with tunable active nitrogen in the carbon framework for simultaneous enhancement of cO_(2) capture capability and Supercapacitance(Sc).The synthesized A-TDP-12 possesses a typical hierarchically porous framework(micro-pores and meso-pores)with a large surface area(1332 m^(2) g^(-1))and a rich content of N(7.89 at.%)and P(0.74 at.%).It delivers a cO_(2) adsorption capacity of 1.52 and 5.68 mmol g^(-1) at 1 and 5 bar,respectively,with almost no decay after successive 8 *** 6 M KOH aqueous electrolyte,A-TDP-12 exhibits a superior specific capacitance of 172.7 F g^(-1) at a current density of 1 A g^(-1).Even at a high current density of 10 A g^(-1),80%of its initial capacity still *** work not only offers a novel strategy for fabricating promising adsorbents and electrodes for cO_(2) uptake and Scs,but also provides new insights into design of porous carbon material for related applications.

关键词： N,P co-doping Porous carbon cO_(2)capture Super-capacitors Bi-function

Seasonal and Spatial Variability of Microparticles in Snowpits on the Tibetan Plateau, china

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Journal of Mountain Science 2010年第1期7卷 15-25页

作者： zhang Yulana KANG Shichang zhang Qianggong cONG Zhiyuan zhang Yongjun GAO Tanguang Laboratory of Tibetan Environment changes and Land Surface Processes Institute of Tibetan Plateau Research Chinese Academy of Sciences Beijing 100085 China State Key Laboratory of cryospheric Sciences ChineseAcademy of Sciences Lanzhou 730000 China Graduate University of chinese Academy of Sciences Beijing 100049 China

The work presents microparticle concentrations in snowpits from the East Rongbuk Glacier on Mt. Qomolangma (Everest) (ER) (28.02°N, 86.96°E, 6536 m a.s.l.), the Zhadang Glacier on Mt. Nyainqentanglha (NQ) (30.47°N, 90.65°E, 5800m a.s.l.), and the Guoqu Glacier on Mt. Geladaindong (GL) (33.95°N, 91.28°E, 5823m a.s.l.) over the Tibetan Plateau (TP). Variations of microparticle and major ions (e.g. Mg2+, ca2+) concentrations in snowpits show that the values of the microparticles and ions in the non-monsoon seasons are much higher than those in the monsoon seasons. Annual flux of microparticle deposition at ER is lower than those at NQ and GL, which could be attributed to the long distance away from the possible dust source regions as well as the elevation for ER higher than the others. compared with other remote areas, microparticle concentrations in the southern TP are much lower than those in the northern TP, but still much higher than those in Greenland and Antarctica. The seasonal and spatial microparticle variations are clearly related to the variations of atmospheric circulation according to the air mass 5-day backward trajectory analyses of HYSPLIT Model. Resultingly, the high microparticle values in snow are mainly attributed to the westerlies and the strong dust storm outbreaks on the TP, while the monsoon circulation brings great amount of precipitation from the Indian Ocean, thus reducing in the aerosol concentrations.

关键词： Microparticle seasonal change spatialvariation snowpits Tibetan Plateau