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Journal of Ocean University of China 2017年第5期16卷 911-915页

作者： SUN Xueping WANG Man liu buming SUN Zhenliang Guangxi Institute of Traditional Medical and Pharmaceutical Science & Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standards Nanning 530022 P. R. China Fengxian Hospital Affiliated to Southern Medical University Shanghai 201499 P R. China Guangxi Institute of Oceanology Guangxi Key Laboratory of Marine Biotechnology Beihai 536000 P R. China

Three angiotensin I converting enzyme(ACE) inhibition peptides were isolated from sandworm Sipunculus nudus protein hydrolysate prepared using protamex. Consecutive purification methods, including size exclusion chromatography and reverse-phase high performance liquid chromatography(RP-HPLC), were used to isolate the ACE inhibition peptides. The amino acid sequences of the peptides were identified as Ile-Asn-Asp, Val-Glu-Pro-Gly and Leu-Ala-Asp-Glu-Phe. The IC_(50) values of the purified peptides for ACE inhibition activity were 34.72 μmol L^(-1), 20.55 μmol L^(-1) and 22.77 μmol L^(-1), respectively. These results suggested that S. nudus proteins contain specific peptides that can be released by enzymatic hydrolysis. This study may provide an experimental basis for further systematic research, rational development and clinical utilization of sandworm resources.

关键词： hydrolysis converting purification exclusion Angiotensin Inhibition shrimp isolate purified Enzyme

Marsdenia tenacissima injection induces the apoptosis of prostate cancer by regulating the AKT/GSK3β/STAT3 signaling axis

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Chinese Journal of Natural Medicines 2023年第2期21卷 113-126页

作者： LI Xiaolan HE Songhua LIANG Wei ZHANG Weiquan CHEN Xin LI Qiaofeng YANG Xin liu Yanying ZHU Dan LI Li liu buming SU Zhiheng CHEN Jie GUO Hongwei Guangxi Key Laboratory of Bioactive Molecules Research and Evaluation&College of Pharmacy Guangxi Medical UniversityNanning 530021China Key Laboratory of Longevity and Aging-related Diseases of Chinese Ministry of Education&Center for Translational Medicine Guangxi Medical UniversityNanning 530021China Guangxi Institute for Food and Drug Control Drug Administration of Zhuang Autonomous RegionNanning 530021China Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standards Guangxi Institute of Chinese Medicine&Pharmaceutical ScienceNanning 530022China The Affiliated Tumor Hospital Guangxi Medical UniversityNanning 530021China

Marsdenia tenacissima injection,a standard Marsdenia tenacissima extract(MTE),has been approved as an adjuvant therapeutic agent for various *** previous study showed that MTE inhibited the proliferation and metastasis of prostate cancer(PCa)***,the underlying mechanisms and active ingredients of MTE against PCa were not completely *** study revealed that MTE induced significant decreases in cell viability and clonal growth in PCa *** addition,MTE induced the apoptosis of DU145 cells by reducing the mitochondrial membrane potential and increasing the expression of Cleaved Caspase 3/7,Cyt c,and *** vivo,DU145 xenografted NOD-SCID mice treated with MTE showed significantly decreased tumor *** staining and Western blot confirmed the pro-apoptotic effects of *** pharmacology analysis collected 196 ingredients of MTE linked to 655 potential targets,and 709 PCa-associated targets were retrieved,from which 149 overlapped targets were screened *** enrichment analysis showed that the HIF-1,PI3K-AKT,and ErbB signaling pathways were closely related to tumor *** blot results confirmed that MTE increased the expression of p-AKT^(Ser473) and p-GSK3β^(Ser9),and decreased the expression of p-STAT3^(Tyr705) in vitro and in vivo.A total of 13 compounds in MTE were identified by HPLC-CAD-QTOF-MS/MS and UPLCQTOF-MS/*** docking analysis indicated that six compounds may interact with AKT,GSK3β,and *** conclusion,MTE induces the endogenous mitochondrial apoptosis of PCa by regulating the AKT/GSK3β/STAT3 signaling axis,resulting in inhibition of PCa growth in vitro and in vivo.

关键词： Marsdenia tenacissima injection Prostate cancer Apoptosis AKT/GSK3β/STAT3

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不同蒸馏方式对岗松精油得油率与成分的影响

广西林业科学 2024年第3期53卷 324-328页

作者：李军集钟德进陈海燕李桂珍刘布鸣梁忠云广西壮族自治区林业科学研究院广西特色经济林培育与利用重点实验室广西木本香料工程技术研究中心广西南宁530002 广西壮族自治区国有钦廉林场广西钦州535099 广西中医药研究院广西南宁530022

为探索蒸馏工艺对岗松(Baeckea frutescens)精油品质的影响,采用水中蒸馏、水上蒸馏、扩散蒸馏、复蒸蒸馏和同时萃取蒸馏5种蒸馏方式提取岗松枝叶精油,对精油得油率和成分进行分析。结果表明,复蒸蒸馏精油得油率最高。采用复蒸蒸馏获取... 详细信息

为探索蒸馏工艺对岗松(Baeckea frutescens)精油品质的影响,采用水中蒸馏、水上蒸馏、扩散蒸馏、复蒸蒸馏和同时萃取蒸馏5种蒸馏方式提取岗松枝叶精油,对精油得油率和成分进行分析。结果表明,复蒸蒸馏精油得油率最高。采用复蒸蒸馏获取岗松精油时,设置液料比8∶1,蒸馏2 h,得油率为1.101%;精油中,β-蒎烯、4-松油醇、1,8-桉叶素和芳樟醇含量均符合广西地方标准DB45/T 1064—2014对特征组分的要求。试验结果可为岗松精油工业化生产提供参考。

关键词：岗松精油蒸馏得油率成分

A Visualization Study of Hot Spots of Research on Siraitiae Fructus over a Decade Based on Co-clustering Analysis

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Medicinal Plant 2018年第1期9卷 1-5,15页

作者： Jianhua FAN Min LIANG buming liu Guangxi Institute of Traditional Medical and Pharmaceutical Science Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standards

[Objectives] To use co-clustering analysis and visualization method to analyze the research on Siraitiae Fructus in recent ten years,to know the hot spots and trend of research. [Methods] Relevant research results about S. Fructus in CNKI from January of 2007 to December of 2016 were retrieved by computers,and the retrieval time was February 20,2017. BICOMB,Net Draw,g CLUTO and SPSS19. 0 software were used to conduct co-clustering analysis and visualization analysis for included articles. Keywords were analyzed,and social network graph,visualization matrix,peak image and multidimensional scaling analysis map were drawn. Correlation among high-frequency key words were analyzed. [Results] Totally 723 articles were included,among which 70 articles were issued during 2012-2016; 76 key words were obtained by key word co-occurrence network map,among which mogroside,MOG,extraction process,tissue culture,cultivation technology,varieties,growth and development were in the core position; visualization and the peak image showed that the topics in this research field could be divided into 6 categories; research hotspot dynamic evolution showed that S. Fructus flower,beverage,total flavonoids,gene expression,gene cloning,enzyme,apoptosis,and S. Fructus seed oil would be the hot spots of further study. [Conclusions]This study reveals that the research on S. Fructus in the recent ten years is becoming mature,and expanding to deep level. This study can be promoted to discipline development evaluation of TCM research field.

关键词： Siraitiae Fructus Co-clustering analysis Visualization BICOMB gCLUTO Ucinet SPSS Data mining

Effect of Fixed Mindset: How does Motivational Climate at Work Influence Employee's Knowledge Hiding?

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Effect of Fixed Mindset: How does Motivational Climate at Wo...

Synthesis of Caffeic Acid Sulphonamide Derivatives and Preliminary Exploration of Their Biological Applications

第二十三届全国心理学学术会议

作者： Yufan Zhou Jianwei Zhang Weijun Hua Wenfeng Zheng buming liu School of Humanities and Social Sciences Beijing Institute of Technology

Previous research suggests that a fixed mindset is positively related to knowledge hiding. However, we argue that this relationship depends on the motivational climate, which reverses the effect of a fixed ***, we explore two motivational climates, namely performance and mastery climate, moderate the relationship between fixed mindset and knowledge hiding, such that under the condition of high-performance climate and high-mastery climate, fixed mindset would have contrary effects on knowledge hiding. Drawing on the social learning theory, we explore the opposite signs of the relationship between fixed mindset and knowledge hiding by examining the boundary condition of two-dimensional motivational climate. We conducted a field study of 461 R&D personnel from 8 enterprises and research institutes in China to test our hypothesized model. The results showed that performance climate negatively moderated the relationship between a fixed mindset and knowledge hiding, and mastery climate positively moderated a fixed mindset and knowledge hiding. Furthermore, we include creativity as an essential outcome of knowledge hiding and explore the distal consequence of the moderation effect of a fixed *** results also demonstrated that performance climate negatively moderated the indirect influence of a fixed mindset on creativity via knowledge hiding, and mastery climate positively moderated the indirect effect of fixed mindset on creativity through knowledge hiding. Such results highlight that the perception of the motivational climate affects the direct relationship between a fixed mindset and knowledge hiding and the indirect relationship between a fixed mindset and creativity. Theoretical and practical implications are also discussed.

关键词： fixed mindset knowledge hiding performance climate mastery climate creativity

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Chemical Research in Chinese Universities 2020年第5期36卷 795-803页

作者： PENG Xiaoyu ZHAO Anran HUANG Kelin HU Tingju liu Burning HUANG Yan CHEN Hailan CHAI Ling LIN Cuiwu School of Chemistry and Chemical Engineeting Guangxi UniversityNanning 530004P.R.China School of Animal Science and Technology Guangxi UniversityNanning 530004P.R.China Laboratory Medicine Department First.Affiliated Hospital of Guangxi Medical UniversityNanning 530021P.R.China Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standards Guangxi Institute of Traditional Medical and Pharmaceutical SciencesNanning 530022P.R.China China Academy of Science and Technology Development Guangxi Branch Nanning 530022P.R.China

Stnictural modification of native compounds is an effective way to develop new dmgs with increased pharmacological activities and improved material *** this study,cafteic acid sulphonamide derivatives(CSs)were synthesised by conjugating sulphonamides to the backbone of cafteic *** structures of these derivatives were elucidated by means of Fourier traiisfonn infrared spectroscopy(FTIR),1H NMR,13C NMR,and electrospray ionization mass spectroscopy(ESI-MS).A content determination method was established by ultraviolet *** lipophilicity,2,2-diphenyl-l-picrylhydrazy free radical(DPPH)scavenging capacity,anti-coagulant eitects,anti-bacterial activity,cytotoxicity,in vitro anti-proliterative activity against tumour cells,and the ability of the compounds to promote the proliferation of chondrocytes were *** results indicate that CSs exliibit strong DPPH scavenging activity,high lipophilicity,good anti-coagulant activities,wide anti-bacterial activity range,low cytotoxicity,and an excellent ability to promote chondrocyte proliferation.

关键词： Cafteic acid Sulphonamide Lipophilicity Anti-oxidant Cytotoxicity

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白千层挥发油化学成分分析

分析测试学报 1999年第6期18卷 70-72页

作者：刘布鸣彭维广西中医药研究所南宁530022

用毛细管气相色谱、气相色谱－质谱－计算机分析技术，对植物白千层枝叶挥发油化学成分进行了分析研究，从这种植物挥发油中分离出８０个以上的组分，确认了其中的３５种成分，所鉴定的组分占挥发油色谱总峰面积的９０％以上。

关键词：白千层挥发油成分分析测定

Th(Ⅳ)盐沉淀-EDTA滴定法快速测定污水中总磷研究

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非金属矿 2008年第5期31卷 38-40页

作者：刘步明王瑞斌榆林学院化学与化学工程学院陕西榆林719000

研究了快速测定污水中磷含量的Th（Ⅳ）盐沉淀-EDTA滴定的分析方法。水样用HNO3及H2SO4消解为PO4^3-并在掩蔽干扰离子后的酸性溶液中，先加入已知量且过量的Th（NO3）4标准溶液．当Th、（PO4）4沉淀完全并在pH2-3滤液中。以二甲酚橙为... 详细信息

研究了快速测定污水中磷含量的Th（Ⅳ）盐沉淀-EDTA滴定的分析方法。水样用HNO3及H2SO4消解为PO4^3-并在掩蔽干扰离子后的酸性溶液中，先加入已知量且过量的Th（NO3）4标准溶液．当Th、（PO4）4沉淀完全并在pH2-3滤液中。以二甲酚橙为指示剂，用乙二胺四乙酸二钠盐（EDTA）标准溶液滴定剩余的Th（NO3）4至终点、测定结果的相对标准偏差小于2．5％，加标回收率在94．7%-101．2％之间。结果表明，改进后的方法简单、快捷、干扰小．测定结果与国家标准方法相符，相对偏差均小于2．0％。

关键词： Th(Ⅳ)盐 EDTA滴定法污水磷测定

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多不饱和脂肪酸在肿瘤防治中的应用

陕西师大学报（自然科学版） 1996年第1期24卷 115-119页

作者：刘步明榆林高等专科学校化学系

讨论了多不饱和脂肪酸对动物、人肿瘤的体内、外抑癌活性；对抑癌机理的研究进行了较系统的综述．

关键词：多不饱和脂肪酸肿瘤抑癌活性脂肪酸治疗

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红千层挥发油的化学成分分析

清华大学学报（自然科学版） 2010年第9期50卷 1437-1439页

作者：刘布鸣董晓敏林霄莫建光张玉贤苏旭广西壮族自治区中医药研究院南宁530022 广西壮族自治区分析测试研究中心南宁530022 广西壮族自治区职业病防治研究院南宁530021

用气相色谱(GC)-质谱(MS)-计算机分析技术对红千层挥发油进行成分分析。采用水蒸气蒸馏法从红千层中提取挥发油,利用气相色谱-质谱联用技术分离鉴定,采用GC峰面积归一化法确定其相对含量。挥发油平均得油率为0.2%;分离出60多个组分;鉴... 详细信息

用气相色谱(GC)-质谱(MS)-计算机分析技术对红千层挥发油进行成分分析。采用水蒸气蒸馏法从红千层中提取挥发油,利用气相色谱-质谱联用技术分离鉴定,采用GC峰面积归一化法确定其相对含量。挥发油平均得油率为0.2%;分离出60多个组分;鉴定了其中的40多种成分,其中1,8-桉叶素占53.46%、松油醇-4占2.66%、α-蒎烯占12.07%、α-松油醇占12.72%、γ-松油烯占1.39%、香叶醇占1.18%等;占色谱总馏出峰面积的90%以上。结果表明:红千层挥发油主要化学成分为蒎烯、桉叶素、松油醇等化合物。

关键词：红千层挥发油气相色谱-质谱联用(GC-MS)