Purification and Characterization of Angiotensin I Converting Enzyme Inhibition Peptides from Sandworm Sipunculus nudus

作     者：SUN Xueping WANG Man LIU Buming SUN Zhenliang 

作者机构：Guangxi Institute of Traditional Medical and Pharmaceutical Science & Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standards Nanning 530022 P. R. China Fengxian Hospital Affiliated to Southern Medical University Shanghai 201499 P R. China Guangxi Institute of Oceanology Guangxi Key Laboratory of Marine Biotechnology Beihai 536000 P R. China 

出 版 物：《Journal of Ocean University of China》 (中国海洋大学学报（英文版）)

年 卷 期：2017年第16卷第5期

页      面：911-915页

核心收录：

学科分类：0710[理学-生物学] 0908[农学-水产] 0707[理学-海洋科学] 09[农学] 0815[工学-水利工程] 0824[工学-船舶与海洋工程] 

基　　金：supported by research grant of Guangxi Key Laboratory Traditional Chinese Medicine Quality Standards (No. GXGZZK201501) the Open Research Fund Program of Guangxi Key Laboratory of Marine Biotechnology (No. GLMBT-201407) partly supported by Shanghai Fengxian District Science and Technology Project (Nos. 20141001 and 20151205) Shanghai No. 6 People’s Medical Group Project and research project of Shanghai municipal health and Family Planning Commission (No. 201540027) 

主　　题：hydrolysis converting purification exclusion Angiotensin Inhibition shrimp isolate purified Enzyme 

摘      要：Three angiotensin I converting enzyme(ACE) inhibition peptides were isolated from sandworm Sipunculus nudus protein hydrolysate prepared using protamex. Consecutive purification methods, including size exclusion chromatography and reverse-phase high performance liquid chromatography(RP-HPLC), were used to isolate the ACE inhibition peptides. The amino acid sequences of the peptides were identified as Ile-Asn-Asp, Val-Glu-Pro-Gly and Leu-Ala-Asp-Glu-Phe. The IC_(50) values of the purified peptides for ACE inhibition activity were 34.72 μmol L^(-1), 20.55 μmol L^(-1) and 22.77 μmol L^(-1), respectively. These results suggested that S. nudus proteins contain specific peptides that can be released by enzymatic hydrolysis. This study may provide an experimental basis for further systematic research, rational development and clinical utilization of sandworm resources.