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Red Onion (Allium Cepa L.) Methanolic Extract Increases Extracellular Nucleotide Hydrolysis in Rat Serum

Open Journal of Applied Sciences 2020年第12期10卷 864-876页

作者： Janayne Luihan Silva bruno quintanilha faria Vinícius Marques Arruda Fernanda Cardoso da Silva Joyce Ferreira da Costa Guerra Cristina Ribas Fürstenau Instituto de Ciências Biológicas Universidade Federal de Minas Gerais Belo Horizonte Brazil Curso de Biotecnologia Universidade Federal de Uberlandia Patos de Minas Brazil Programa de Pós-graduaç o em Biotecnologia Instituto de Biotecnologia Universidade Federal de Uberlandia Patos de Minas Brazil Centro de Ciências Naturais e Humanas Universidade Federal do ABC Santo André Brazil

Ectonucleotidases are enzymes involved in nucleotides metabolism. The amount of circulating nucleotides may modulate distinct pathophysiological processes in the vasculature, including platelet aggregation and vascular tone. Onion (Allium cepa L.) phenolic compounds modulate enzymatic activity. The present study evaluated the total phenolic content of red onion methanolic extract, its antioxidant capacity, and its ability to interfere in nucleotides hydrolyses in rat serum. Total phenolic content was determined with the Folin-Ciocalteau reagent using gallic acid as a standard, while total flavonoid content was obtained through the aluminum chloride colorimetric method with quercetin as a standard. Antioxidant capacity was evaluated from the ability of the extract to scavenge ABTS·+ and DPPH· radicals. ATP, ADP, AMP, and p-Nph-5'-TMP hydrolyses were colorimetrically determined in response to different onion extract concentrations (0, 125, 250, 500, or 1000 μg/mL). Phenolic content of the extract was 54.35 mg GAE 100 g-1 sample, while flavonoid content was 7.22 mg quercetin g-1 sample. The IC50 value for ABTS·+ was 374.13 ± 7.52 μg/mL, while it was 440.29 ± 15.17 μg/mL for DPPH·. Red onion extract increased ADP and p-Nph-5'-TMP hydrolysis. The results confirmed that red onion contains high content of antioxidant, mainly flavonoids, and high antioxidant capacity. Additionally, biochemical studies suggest that the increased ADP breakdown may be important to regulate vascular processes. As it occurs for other enzymes, the antioxidant capacity of onion extract may neutralize reactive oxygen species (ROS) formation and favor ectonucleotidase activity and the hydrolysis of ADP, a major platelet agonist.

关键词： Red Onion Flavonoids Reactive Oxygen Species Ectonucleotidases Vascular Homeostasis

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Influence of fire on woody vegetation of savanna and forest formations in the Cerrado biome

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林业研究:英文版 2023年第5期34卷 1207-1216页

作者： Aline das Gracas Costa Fillipe Tamiozzo Pereira Torres Gumercindo Souza Lima Fabiano Rodrigues de Melo Carlos Moreira Miquelino Eleto Torres bruno Leao Said Schettini Vicente Paulo Santana Neto AndréLuiz Lopes de faria Department of Forest Engineering Federal University of VicosaUFVVicosaMG 36570-900Brazil Department of Geography Federal University of VicosaUFVVicosaMG 36570-900Brazil

Wildfires have environmental,economic,and social impacts,and can shape the landscape and benefit ecosystems such as the *** study evaluated the diversity,similarity,and floristic and structural differences of woody savanna and forest formations of the Cerrado,when affected and not affected by ***-eight 25-400m2 plots were randomly allocated and divided into Burnt Cerrado and Unburnt Cerrado,Burnt Forest,and Unburnt Forest,and divided into three levels of inclusion according to diameter class(smaller than 2 cm,2-5 cm and larger than5 cm).Species were identified,DBH and height measured,and phyto sociological parameters such as volume,diversity,and floristic similarity *** Cerrado had lower diversity,density,and dominance at all inclusion levels compared to the Unburnt Cerrado,and showed similarities between treatments at the inclusion *** Forest had smaller differences in diversity,density,and dominance than Unburnt *** formation was similar at level 1 of inclusion and in the total ***,it was dissimilar at lower *** lower density and diversity of species at the lowest levels of inclusion was associated with mortality from fire.

关键词： Biodiversity Brazil Burning Floristic Phytosociology

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A Contribution to the Analysis of Frequency Response and Impedance Measurements to Evaluation and Diagnosis of Power Transformers

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高电压技术 2008年第12期34卷 2655-2661页

作者： Helvio J. A. Martins Cintia de faria Ferreira bruno Urbani CEPEL-Electric Power Research Center Av. Horacio Macedo 354-Cidade Universitaria Zip Code 21941-911-Rio de Janeiro RJ Brazil

This paper has an objective to show a developed quantitative criterion,based in two mathematical variables that explicit the deviation degree of a normal situation,applying simultaneously data from terminal impedances and frequency *** in more than 100-measured equipment,of different applications(step-up transformer,transmission transformer,etc.,),for a period of 10 years,the work presents some examples of practical application of this methodology in Brazilian Electrical System.

关键词：变压器评估诊断频响阻抗传输函数关联系数

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Correction to:Effects of chemical sterilization of the culture media,porous membranes and luminosity on in vitro culture of Eucalyptus grandis×Eucalyptus urophylla

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Journal of Forestry Research 2022年第5期33卷 1705-1707页

作者： Letícia Vaz Molinari Denys Matheus Santana Costa Souza Maria Lopes Martins Avelar Sérgio bruno Fernandes Douglas Santos Gonçalves Júlio Cézar Tannure faria Dulcineia de Carvalho Gilvano Ebling Brondani Department of Forestry Sciences Federal University of LavrasLavrasMinas GeraisBrazil

Correction to:***://***/10.1007/s11676-020-01240-5 In the original publication of the article,there were errors in the fig 3 and fig *** corrected Figs.3 and 6 are given below:The original article has been corrected.

关键词： corrected porous media

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Cubosome nanoparticles for enhanced delivery of mitochondria anticancer drug elesclomol and therapeutic monitoring via sub-cellular NAD(P) H multi-photon fluorescence lifetime imaging

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Nano Research 2019年第5期12卷 991-998页

作者： Ana R.faria Oscar F.Silvestre Christian Maibohm Ricardo M.R.Adao bruno F.B.Silva Jana B.Nieder Department of Nanophotonics Ultrafast Bio- and Nanophotonics GroupINL - International Iberian Nanotechnology LaboratoryAv.Mestre Jose Veiga s/n4715-330 BragaPortugal Department of Life Sciences INL-International Iberian Nanotechnology LaboratoryAv.Mestre Jose Veiga s/n4715-330 BragaPortugal

Elesclomol (ELC) is an anticancer drug inducing mitochondria cytotoxicity through reactive oxygen ***,for the first time,we encapsulate the poorly water soluble ELC in monoolein-based cubosomes stabilized with Pluronic *** uptake and nanocarrier accumulation close to the mitochondria with sub-micrometer distance is identified via three-dimensional (3D) confocal microscopy and edge-to-edge compartment *** monitor the therapeutic effect of the ELC nanocarrier,we apply for the first time,label-free time-lapse multi-photon fluorescence lifetime imaging microscopy (MP-FLIM) to track NAD(P)H cofactors with sub-cellular resolution on live cells exposed to an anticancer *** in vitro cytotoxicity is verified when loading the pre-complexed ELC with copper (ELC-Cu).Importantly,for equivalent copper concentration,cubosomes loaded with ELC-Cu show higher cytotoxicity compared to the free *** novel nanocarrier shows promising features for systemic ELC-Cu administration,and furthermore we establish the MP-FLIM technique for the assessment of anticancer drug delivery systems.

关键词： cubosomes mitoch on dria reactive oxyge n species elesclomol NAD(P)H fluoresce nee lifetime anticancer therapy

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Effects of chemical sterilization of the culture media,porous membranes and luminosity on in vitro culture of Eucalyptus grandis×Eucalyptus urophylla

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Journal of Forestry Research 2021年第4期32卷 1587-1598页

Hybrid combinations of Eucalyptus have increased due to expansion of plantations into unconventional areas and to the search for higher quality ***,most of these species have difficulties surviving in vitro *** chlorine and sealing systems are often used to reduce contamination and increase gas *** aim of the present study is to evaluate the establishment,multiplication,elongation and adventitious rooting of *** × *** clones(C1 and C2) and four active chlorine concentrations(0.000%,0.001%,0.003%,and 0.005%) were tested in the establishment and multiplication *** sealing forms(W/M,1/M and 3/M) and the same four active chlorine concentrations were applied to the elongation *** luminosities(dark and light)and three sealings(W/M,1/M and 3/M) were tested during adventitious *** chlorine concentration of0.005% led to the lowest fungal contamination rate and to the highest in vitro *** chlorine concentration of 0.003% resulted in the greatest length and highest number of shoots per explant in the multiplication *** were no phytotoxicity problems and the quality of plants grown in an environment with active chlorine was maintained in comparison with those grown in an *** increase in gas exchange in ventilation systems had a positive impact on the in vitro growth and development of plants.

关键词： Asepsis Cloning Gas exchange In vitro propagation

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Bovine Bone Charcoal as Support Material for Immobilization of Bacillus firmusStrain 37 and Production of Cyclomaltodextrin Glucanotransferase by Batch Fermentation in a Fluidized Bed

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Advances in Chemical Engineering and Science 2018年第1期8卷 11-25页

作者： Larissa Albunio Silva bruno Cesar Bieli Osvaldo Valarini Junior Graciette Matioli Gisella Maria Zanin Flavio faria Moraes Chemical Engineering Department State University of MaringaMaringáBrazil Graduate Program of Food Science Universidade Estadual de MaringáMaringáBrazil Pharmaceutical Sciences Department State University of MaringaMaringáBrazil

The process described in the present work uses air supplementation in a fluidized bed reactor containing Bacillus firmus strain 37 immobilized on active bovine bone charcoal, to produce by batch fermentation the enzyme CGTase (cyclomaltodextrin-glucanotransferase). Three different aeration rates were evaluated. The maximum CGTase activity was achieved after 120 hours of fermentation with aeration rate of 2 vvm and was equal to 2.48 U/mL. When 0.5 and 1 vvm were used the enzymatic activities achieved 1.1 and 0.57 U/mL, respectively. Bovine bone charcoal was characterized in terms of surface area, pore size and volume. To the best of our knowledge, the immobilization of microorganism cells in bovine bone charcoal for CGTase production has not been reported in the literature. Our results showed that fluidized bed reactor allows retaining high concentration of biomass, improving biomass-substrate contact and operation at low residence times, which resulted in improved enzyme production. Therefore, the process as proposed has great potential for industrial development.

关键词： Bacillus firmus Bone Charcoal Matrix Cell Immobilization Microbial Enzyme Cyclomaltodextrin Glucanotransferase

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Resistance of Two Maize Landraces in Breeding Stage to the Attack of Sitophilus zeamais

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American Journal of Plant Sciences 2014年第20期5卷 2929-2934页

作者： Rejane Teixeira do Nascimento bruno Ettore Pavan Luciana Barboza Silva Gabriel dos Santos Carvalho Alexandre faria da Silva Kellen Maggioni Graduate Program in Agronomy-Crop Science Federal University of Piauí Bom Jesus Brazil College of Engineering of Ilha Solteira—DFTASE—UNESP S&atildeo Paulo Brasil

This study aimed to assess the resistance of grain from two varieties of maize landraces in initial breeding in Bom Jesus-PI, with respect to S. zeamais attack. Seeds of two maize landraces were used: purple straw maize from two different origins (São Paulo (SP) and Espírito Santo (ES)) and Peruvian purple maize. The experimental design was completely randomized with four replications. The data for each response variable were subjected to analysis of variance, applying the F-test (p ≤ 0.05), and when significant differences were found, there was a comparison of means by Tukey test (p ≤ 0.05). The statistical analyses were performed using the software SAS®. In a free-choice test and bioassay for evaluating the nutritional index (no choice test), maize varieties differed significantly by Tukey test and the cultivar SP was considered resistant, while the cultivars purple and ES were considered susceptible.

关键词： Selection Genetic Variability Susceptibility

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Comparative Study of Helicoverpa armigera(Hubner) (Lepidoptera: Noctuidae) Preference for Bt and Non-Bt Soybean Cultivars

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American Journal of Plant Sciences 2017年第10期8卷 2483-2500页

作者： Vilmar Bueno Dos Santos Luciana Barboza Silva Eliane Carneiro Alexandre faria Silva Gleidyane Novais Lopes bruno Ettore Pavan Thiago Ferreira Rodrigues Postgraduate Program in Plant Science Agronomy Universidade Federal do Piauí (UFPI) Bom Jesus Piauí FacultyofEngineering UNESP Ilha Solteira Universidade Estadual Paulista Ilha Solteira Brasil

Studies on the behavior of Helicoverpa armigera in relation to soybean cultivars expressing Bt proteins are fundamentally important for IPM. We determine the no-choice and feeding choice of H. armigera, the no-choice and oviposition choice in different Bt and non-Bt soybeans cultivars. In the first step it was carried out the experiment to determine the no-choice and food choice in test for 12 Bt and non-Bt soybean cultivars. Simultaneously the consumption was determined. In a second step it was evaluated the no-choice and oviposition choice in different soybean cultivars. Helicoverpa armigera caterpillars showed neither attractiveness and preference for food nor attractiveness and oviposition preference for Bt and non-Bt soybean leaves when simultaneously contrasting the tested cultivars. This study showed that Bt toxin did not influence the oviposition preference, and H. armigera adults did not differentiate Bt and non-Bt soybean cultivars for oviposition indicating no behavioral preference. Third instar caterpillars showed no discrimination between Bt and non-Bt soybean leaves. Research in this area must focus on the possibility of widespread planting of genetically modified soybeans containing the Bt protein, which is associated with selection pressure and the behavior of pest species in relation to their hosts, as well as on the adequacy of management tactics that is able to prevent the loss of technology efficiency.

关键词： Cry1Ac Protein Genetically Modified Plants Food Preference Oviposition

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ID7 Isolated from Bauhinia variegata Stem Inhibits Tumor Progression and Metastatic Mechanisms of Triple Negative Breast Cancer in Vivo

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Journal of Pharmacy and Pharmacology 2019年第7期7卷 368-384页

作者： Kamilla Monteiro dos Santos Izabela Natalia faria Gomes Renato JoséSilva Oliveira Fernanda E.Pinto bruno Oliveira Fabrício P.Batista Rafael Cesar Russo Chagas Hélio B.Santos Ralph G.Thomé Wanderson Romao Rui Manuel Reis Maria Luiza Vilela Oliva Rosy Iara Maciel de Azambuja Ribeiro Laboratory of Experimental Pathology Federal University of Sao Joao del-Rei(UFSJ)DivinópolisMGBrazil Josédo Rosário Vellano University UnifenasDivinópolisMGBrazil Molecular Oncology Research Center(CPOM) Barretos Cancer HospitalBarretosSPBrazil Petroleomic and Forensic Chemistry Laboratory Department of ChemistryFederal Institute of Espírito Santo(IFES)VitóriaESBrazil Chemistry Department Federal University of Sao PauloBrazil Tissue Processing Laboratory Federal University of Sao Joao del-Rei(UFSJ)DivinópolisMGBrazil Life and Health Sciences Research Institute(ICVS) Medical SchoolUniversity of MinhoBragaPortugal 3ICVS/3B’s-PT Government Associate Laboratory GuimaraesBragaPortugal

Background:The breast cancer has been the most common form of cancer among *** triple negative subtype represents 20%of all breast cancer cases in the world and is standing out by affecting young women and being *** main cause of death of patients with cancer is due to metastasis,which can reach the liver and ***:The activities of ID7 fraction of the stems of Bauhinia variegata *** breast cancer,lung metastasis and liver inflammatory process were ***:ID7 was characterized by *** viability of murine mammary cells(4T1)treated with ID7 was assessed by MTT,trypan blue and fluorescence assay and viability of BT-20,MDA-MB-231 and MCF-7 human breast cancer tumor lines by *** cell migration,invasion using matrigel and adhesion were *** expression of cell death proteins was quanitified by western blot and the gelatinases by *** ID7 activity of the tumor(4T1)and metastatic progession in vivo was ***:ID7 reduced the 4T1 and MDA-MB-231viability and increased the late apoptosis,inhibited the 4T1 migration and invasion,increased the 4T1 adhesion and decreased the secreted active ***7 also increased the expression of PARP,caspase-7 and caspase-8,RIP and *** vivo,the ID7 decreased the volume and weight of the tumors and decreased lung metastasis and inflammation in the *** characterization showed mainly the presence of oleic acid,myricetin,quercetin and kaempferol in ***:Thus,it was found that ID7 fraction exhibits selective antitumor and on the mechanisms of breast cancer metastasis activity,preventing lung metastasis and inflammation in the *** is suggested that fatty acids and flavonoids are correlated with such activities.

关键词： 4T1 cells MDA-MB-231 medicinal plants kaempferol gelatinases apoptosis cell viabilty in vivo

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