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Observation of granulations in the basal body of ovarioles and follicular dilatations for the determination of physiological age of Anopheles gambiae s.s.

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Journal of Coastal Life Medicine 2015年第7期 526-530页

作者： Rodrigue Anagonou Virgile Gnanguenon Fiacre Agossa bruno akinro Armand Akpo Martial Gbegbo Albert Salako Martin Akogbéto Center of Entomological research of Cotonou 06 BP 2604 CotonouBenin Faculty of Sciences and Techniques University of Abomey CalaviCalaviBenin

Objective:To explore ovariole basal body granulations and follicular dilatations for determining physiological age inAnopheles gambiaes.s.(***.s.).Methods:Mosquitoes were collected by using window trap catch and identified *** the first lot ofmosquitoes,they were dissected,and ovary was left in distilled water for reading ovarian tracheoles and the second was cut and transferred to another blade in a physiological liquid for verification of ovariole basal body *** same approach was followed with the second lot of mosquitoes where follicular dilatations were found after classic dilaceration of ovaries were transferred into physiological *** other body parts of mosquitoes were used to identify the species of *** by ***:Among the *** *** the first lot,the method of Detinova determined the age of 89 mosquitoes versus 114 for the observation of granulations(P>0.05).Among the *** *** the second lot,the method of Detinova determined the age of 84 mosquitoes versus 93 for the observation of follicular dilatations(P>0.05).Unlike the method of Detinova,observation of follicular dilatations and basal body granulations of ovarioles were possible beyond the stage II ***:Overall,the observation of follicular dilatations and ovariole basal body granulations are reliable for the determination of the physiological age ***,these two methods can be used beyond the stage II.

关键词： Anopheles gambiae s.s. Physiological age Ovarioles Dilatations Granulations

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