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Staining for p53 and Ki-67 increases the sensitivity of eUS-FNA to detect pancreatic malignancy

World Journal of Gastrointestinal endoscopy 2010年第11期2卷 362-368页

作者： Alexander W Jahng Sonya Reicher David Chung Donna Varela Rahul Chhablani Anil Dev Binh Pham Jose Nieto Rose J Venegas Samuel W French bruce e stabile Viktor e eysselein Division of Gastroenterology Harbor-UCLA Medical CenterTorranceCA 90502United States Department of Pathology Harbor-UCLA Medical CenterTorranceCA 90502United States Department of Surgery Harbor-UCLA Medical CenterTorranceCA 90502United States

AIM:To investigate whether tumor marker staining can improve the sensitivity of endoscopic ultrasound-guided fine needle aspiration(eUS-FNA)to diagnose pancreatic malignancy. MeTHODS:Patients who underwent eUS-FNA were retrospectively *** eUS-FNA specimen was evaluated by routine cytology and stained for tumor markers p53,Ki-67,carcinoembryonic antigen(CeA) and ***,specificity,positive and negative predictive values(PPV and NPV),and positive and negative likelihood ratios(PLR and NLR)were calculated in order to evaluate the performance of each test to detect malignancy. ReSULTS:Sixty-one specimens had complete sets of stains,yielding 49 and 12 specimens from pancreatic adenocarcinomas and benign pancreatic lesions due to pancreatitis,*** alone had sensitivity and specificity of 41%and 100%to detect malignancy, *** 46%of the specimens,routine cytology alone was deemed *** addition of either p53 or Ki-67 increased the sensitivity to 51%and 53%,respectively,with perfect specificity,PPV and PLR (100%,100%and infinite).Both stains in combination increased the sensitivity to 57%.While additional staining with CeA and CA19-9 further increased the sensitivity to 86%,the specificity,PPV and PLR were significantly reduced(at minimum 42%,84%and 1,respectively).Markers in all combinations performed poorly as a negative test(NPV 26%to 47%,and NLR 0.27 and 0.70).CONCLUSION:Immunohistochemical staining for p53 and Ki-67 can improve the sensitivity of eUS-FNA to diagnose pancreatic adenocarcinoma.

关键词： endoscopic ultrasound Fine needle aspira tion Pancreatic cancer p53 Ki-67 Immunohistochemistry

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Complex ammonium oxidation demands visualized resolution

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Science Bulletin 2024年第16期69卷 2478-2482页

作者： Zhihao Chen Yonghong Wu Jan Dolfing Shunyao Zhuang Baozhan Wang Dan Li Shan Huang bruce e.Rittmann State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil ScienceChinese Academy of SciencesNanjing 210008China Zigui Three Gorges Reservoir ecosystem Observation and Research Station of Ministry of Water Resources of the People’s Republic of ChinaYichang 443605China University of Chinese Academy of Sciences Beijing 100049China d College of Nanjing University of Chinese Academy of SciencesNanjing 211135China Faculty of energy and environment Northumbria UniversityNewcastle-upon-Tyne NE18QHUK Department of Microbiology College of Life SciencesNanjing Agricultural UniversityKey Laboratory of Agricultural and Environmental MicrobiologyMinistry of Agriculture and Rural AffairsNanjing 210095China College of Urban Construction Nanjing Tech UniversityNanjing 211816China Department of Civil and environmental engineering Princeton UniversityPrinceton NJ 08540USA Biodesign Swette Center for environmental Biotechnology Arizona State UniversityTempe AZ 85287-5701USA

The nitrogen cycle plays a vital role in sustaining productive and healthy ecosystems,with the microbial nitrogen cycle being a focal point in agriculture and eco-environmental *** these microbially mediated processes is ammonium(NH_(4)^(+))oxidation,the gatekeeper of the nitrogen *** understanding of ammonium oxidation is continuously evolving,thanks to recent research breakthroughs like comammox,Feammox,and dirammox[1-6].The increasing complexity of ammonium oxidation necessitates more precise and well-focused research tools beyond those commonly used today.

关键词： ammonium breakthrough precise

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The new kid on the block in insect pest management:sprayable RNAi goes commercial

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Science China(Life Sciences) 2024年第8期67卷 1766-1768页

作者： Junjie Yan Ralf Nauen Stuart Reitz Andrei Alyokhin Jiang Zhang David Mota-Sanchez Yonggyun Kim Subba Reddy Palli Silvia I.Rondon Brian A.Nault Juan Luis Jurat-Fuentes Michael S.Crossley William e.Snyder Angharad M.R.Gatehouse Myron P.Zalucki bruce e.Tabashnik Yulin Gao State Key Laboratory for Biology of Plant Disease and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China Bayer AG Crop Science DivisionR&DPest ControlMonheim 40789Germany Department of Crop and Soil Science Oregon State UniversityOntario 97914USA School of Biology and ecology University of MaineOrono 04469USA Shenzhen Branch Guangdong Laboratory of Lingnan Modern AgricultureKey Laboratory of Synthetic BiologyMinistry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518000China Department of entomology Michigan State UniversityEast Lansing 48864USA Department of Plant Medicals Andong National UniversityAndong 36729Republic of Korea Department of entomology University of KentuckyLexington 40503USA Oregon State University Oregon Integrated Pest Management CenterCorvallis 97838USA Department of entomology Cornell UniversityCornell AgriTechGeneva 14456USA Department of entomology and Plant Pathology University of TennesseeKnoxville 37996USA Department of entomology and Wildlife ecology University of DelawareNewark 19716USA Department of entomology University of GeorgiaAthensGeorgia 30602USA School of Natural and environmental Sciences Newcastle UniversityNewcastle upon TyneNE17RUUK School of the environment University of QueenslandBrisbane 4072Australia Shandong engineering Research Center for environment-Friendly Agricultural Pest Management College of Plant Health and MedicineQingdao Agricultural UniversityQingdao 266109China Department of entomology University of ArizonaTucson 85721USA National Center of excellence for Tuber and Root Crop Research Chinese Academy of Agricultural SciencesBeijing 100081China

Insect pest damage to crops is a threat to global food security(Tilman et al.,2011).Climate change,the evolution of insecticide resistance,and the phasing out of insecticides due to environmental and safety concerns exacerbate this *** urgently need safe and effective crop protection tools to sustainably generate yields that meet ever-increasing global demand.

关键词： crops damage pest

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Multinuclear MR and MRI study of lithium-ion cells using a variable field magnet and a fixed frequency RF probe

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Magnetic Resonance Letters 2024年第1期4卷 10-20页

作者： Andrés Ramírez Aguilera Florin Marica Kevin J.Sanders Md Al Raihan C.Adam Dyker Gillian R.Goward bruce *** UNB MRI Centre Department of PhysicsUniversity of New BrunswickFrederictonNew BrunswickE3B 5A3Canada Department of Chemistry McMaster UniversityHamiltonOntarioL8S 4L8Canada Department of Chemistry University of New BrunswickFrederictonNew BrunswickE3B 5A3Canada

An exploratory multinuclear magnetic resonance(MR)and magnetic resonance imaging(MRI)study was performed on lithium-ion battery cells with ^(7)Li,^(19)F,and ^(1)H measurements.A variable field superconducting magnet with a fixed frequency parallel-plate radiofrequency(RF)probe was employed in the *** magnetic field was changed to set the resonance frequency of each nucleus to the fixed RF probe frequency of 33.7 *** cartridge-like lithium-ion cells,with graphite anodes and LiNi_(0.5)Mn_(0.3)Co_(0.2)O_(2)(NMC)cathodes,were *** cell was pristine,and one was charged to a cell voltage of 4.2 *** results presented demonstrate the great potential of the variable field magnet approach in multinuclear measurement of lithium-ion *** methods open the door for developing faster and simpler methods for detecting,quantifying,and interpreting MR and MRI data from lithium-ion and other batteries.

关键词： Multinuclear MR/MRI Variable field magnet Lithium-ion battery Parallel-plate rf probe

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In memory of Perry L. McCarty, seminal founder of environmental Biotechnology

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Frontiers of environmental Science & engineering 2023年第10期17卷 171-173页

作者： bruce e.Rittmann Biodesign Swette Center for environmental Biotechnology Arizona State UniversityTempeAZ 85287-5701USA

Perry *** passed away on June 4,at the age of *** was a revered leader in environmental engineering and *** *** passed away peacefully and with his family on June 4,*** was a world leader in environmental engineering,with special emphasis on environmental Biotechnology,in which we form partnerships with microbial communities that provide services to make human society more sustainable.I was one of Perry’s ***,and we worked together on research,service,and publishing throughout my career.I have deep and personal knowledge of how Perry and his students developed the fundamental principles that have allowed environmental Biotechnology to flourish and advance humans’sustainability.

关键词： peace allowed passed

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Adding Value to Crop Production Systems by Integrating Forage Cover Crop Grazing

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American Journal of Plant Sciences 2024年第3期15卷 180-192页

作者： Robert B. Mitchell Daren D. Redfearn Kenneth P. Vogel Terry J. Klopfenstein Galen erickson P. Stephen Baenziger bruce e. Anderson Mary e. Drewnoski Jay Parsons Steven D. Masterson Marty R. Schmer Virginia L. Jin USDA-ARS Wheat Sorghum and Forage Research Unit University of Nebraska-Lincoln Lincoln NE USA Department of Agronomy and Horticulture University of Nebraska-Lincoln Lincoln NE USA Department of Animal Science University of Nebraska-Lincoln Lincoln NE USA Department of Agricultural economics University of Nebraska-Lincoln Lincoln NE USA USDA-ARS Agroecosystem Management Research Unit University of Nebraska-Lincoln Lincoln NE USA

In addition to their value as cereal grains, wheat (Triticum aestivum L.) and triticale (× Triticosecale Wittmack) are important cool-season annual forages and cover crops. Yearling steer (Bos taurus) performance was compared in the spring following autumn establishment as for age cover crops after soybean [Glycine max (L.) Merr.] grain harvest. Replicated pastures (0.4 ha) were no-till seeded in three consecutive years into soybean stubble in autumn, fertilized, and grazed the following spring near Ithaca, Ne, USA. each pasture (n = 3) was continuously stocked in spring with four yearling steers (380 ± 38 kg) for 17, 32, and 28 d in 2005, 2006, and 2007, respectively. In 2005, average daily gain (ADG) for steers grazing triticale exceeded the ADG for wheat by 0.31 kghd-1d-1. In 2006, wheat ADG exceeded that for triticale by 0.12 kghd-1d-1. In 2007, steers grazing wheat lost weight, while steers grazing triticale gained 0.20 kghd-1d-1. Based on the 3-year average animal gains valued at $1.32 kg-1, mean net return ($ ha-1 yr-1) was $62.15 for triticale and $22.55 for wheat. Since these grazed cover crops provide ecosystem services in addition to forage, grazing could be viewed as a mechanism for recovering costs and adds additional value to the system. Based on this 3-year grazing trial, triticale was superior to wheat and likely will provide the most stable beef yearling performance across years with variable weather for the western Cornbelt USA.

关键词： Cover crops Soybean Triticale Wheat

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Impacts of bovine spongiform encephalopathy and avian influenza on U.S. meat demand

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Journal of Integrative Agriculture 2015年第6期14卷 1130-1141页

作者： Jianhong e Mu bruce A McCarl Amy Hagerman David Bessler Department of Applied economics Oregon State University Department of Agricultural economics Texas A&M University Center for epidemiology and Animal Health Veterinary Services Animal and Plant Health Inspection Service United States Department of Agriculture

This paper examines the U.S. meat demand impacts of the announced outbreaks of bovine spongiform encephalopathy（BSe） and avian influenza（AI）. Findings indicate that beef and chicken demand was negatively affected by BSe and AI disease outbreaks. Specifically, in the short run, U.S. consumers shift demand due to both outbreaks but more so due to domestic disease outbreaks than for outbreaks occurring overseas-the impact of U.S. AI outbreaks is about 0.5% for beef and the impact of U.S. BSe cases is around –0.42% for beef and 0.4% for pork, respectively. Regarding the BSe shock on meat demand, there is a high rate of beef demand adjusted from disturbance to the long-run equilibrium and a lower adjustment rate for chicken demand because of the repeated outbreaks of AI worldwide. In the long run, information related to severe, persistently recurring overseas animal disease outbreaks changes U.S. consumers＇ meat consumption patterns. Although effects of animal diseases on U.S. meat demand were statistically significant, the magnitudes were small-the impact of WHO reported human death numbers for AI is 0.005% for beef, –0.002% for pork, and –0.006% for chicken and the impact of U.S. BSe cases is 1.1% for pork and –0.7% for chicken.

关键词： spongiform avian chicken bovine encephalopathy overseas disturbance adjustment pathogenic deaths

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Bioreduction of vanadium(V) in groundwater by autohydrogentrophic bacteria:Mechanisms and microorganisms

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Journal of environmental Sciences 2015年第4期27卷 122-128页

作者： Xiaoyin Xu Siqing Xia Lijie Zhou Zhiqiang Zhang bruce e.Rittmann State Key Laboratory of Pollution Control and Resource Reuse College of Environmental Science and Engineering Tongji University Swette Center for environmental Biotechnology Biodesign Institute at Arizona State University Key Laboratory of Yangtze River Water environment Ministry of Education College of Environmental Science and EngineeringTongji University

As one of the transition metals, vanadium（V）（V（V）） in trace amounts represents an essential element for normal cell growth, but becomes toxic when its concentration is above 1 mg/L. V（V） can alter cellular differentiation, gene expression, and other biochemical and metabolic phenomena. A feasible method to detoxify V（V） is to reduce it to V（IV）, which precipitates and can be readily removed from the water. The bioreduction of V（V） in a contaminated groundwater was investigated using autohydrogentrophic bacteria and hydrogen gas as the electron donor. Compared with the previous organic donors,H2 shows the advantages as an ideal electron donor, including nontoxicity and less production of excess biomass. V（V） was 95.5% removed by biochemical reduction when autohydrogentrophic bacteria and hydrogen were both present, and the reduced V（IV）precipitated, leading to total-V removal. Reduction kinetics could be described by a first-order model and were sensitive to p H and temperature, with the optimum ranges of p H 7.5–8.0 and 35–40°C, respectively. Phylogenetic analysis by clone library showed that the dominant species in the experiments with V（V） bioreduction belonged to theβ-Proteobacteria. Previously known V（V）-reducing species were absent, suggesting that V（V）reduction was carried out by novel species. Their selective enrichment during V（V）bioreduction suggests that Rhodocyclus, a denitrifying bacterium, and Clostridium, a fermenter known to carry out metal reduction, were responsible for V（V） bioreduction.

关键词： Vanadium Bioreduction Hydrogen Rhodocyclus Clostridium

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Improved nitrogen removal in dual-contaminated surface water by photocatalysis

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Frontiers of environmental Science & engineering 2012年第3期6卷 428-436页

作者： Yongming ZHANG Rong YAN Zhen ZOU Jiewei WANG bruce e.RITTMANN Deparlxnent of environmental engineering College of Life and Environmental ScienceShanghai Normal UniversityShanghai 200234China Swette Center for environmental Biotechnology Biodesign InstituteArizona State UniversityTempeAZ85287-5801USA

River waters in China have dual contamination by nutrients and recalcitrant organic compounds. In principle, the organic compotmds could be used to drive denitrification of nitrate, thus arresting eutrophication potential, if the recalcitrant organics could be made bioavailable. This study investigated the potential to make the recalcitrant organics bioavailable through photocatalysis. Batch denitrification tests in a biofilm reactor demonstrated that dual-contaminated river water was short of available electron donor, which resulted in low total nitrogen （TN） removal by denitrification. However, the denitrification rate was increased signifi- cantly by adding glucose or by making the organic matters of the river water more bioavailable through photocata- lysis. Photocatalysis for 15 min increased the Chemical Oxygen Demand （COD） of the river water from 53 to 84 mg. L-1 and led to a 4-fold increase in TN removal. The increase in TN removal gave the same effect as adding 92 mg.L 1 of glucose. During the photocatalysis experi- ments, the COD increased because photocatalysis trans- formed organic molecules from those that are resistant to dichromate oxidation in the COD test to those that can be oxidized by dichromate. This phenomenon was verified by testing photocatalysis of pyridine added to the river water. These findings point to the potential for N removal via denitrification after photocatalysis, and they also suggest that the rivers in China may be far more polluted than indicated by COD assays.

关键词： dual contamination eutrophication photocatalysis remediation surface water

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Architectural and personal influences on neishborin8 behaviors;

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Frontiers of Architectural Research 2016年第2期5卷 194-201页

作者： bruce K. Fersuson Derek e. Ferguson 1027 Parkview Bird Pittsburgh PA 15217 USA Virginia Commonwealth University Richmond VA 23284 USA

Architectural and personal influences on neighboring behaviors were studied in a residential neighborhood using both qualitative informal conversations, and systematic recording of activity in the neighborhood＇s social space. This dual approach produced new insights into neighboring behaviors and social networks. It was discovered that the residents who participated in the social space were only a portion of the resident population. There was an additional neighborhood-based network whose neighboring was not conducted in the social space; instead it was maintained by direct house-to-house contact. It was also found that some individuals chose not to participate in any neighborhood social network. The social space was an effective neighboring venue for those residents who chose to use it, but did not attract commingling of groups. Contrary to an assumption in previous neighboring research, there are social groups which develop and maintain themselves without participation in a social space.

关键词： Neighborhood Social network Community Residential design Shared space

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