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The first cavefish in the d.naric Karst?Cave colonization mad. possible by phenotypic plasticity in Telestes karsticus

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Zoological Research 2023年第4期44卷 821-833页

作者： MateoČupić Zoran Marčić Marko Lukić Romana gra.a. Helena Biland.ija d.vision of Molecular Biology Ruđer BoškovićInstituteZagreb 10000Croatia d.partment of Biology Faculty of ScienceZagreb 10000Croatia Croatian Biospeleological Society Zagreb 10000Croatia

Cave animals are an excellent mod.l system for stud.ing ad.ptive *** present,however,little is known about the mechanisms that enable surface colonizers to survive in the challenging environment of *** possibility is that these species have the necessary genetic background.to respond.with plastic changes to the pressures of und.rground.*** gain insight into this process,we cond.cted.a comparative stud. with the fish species Telestes karsticus,which occurs in a hyd.ological system consisting of an interconnected.stream and.a *** showed.that *** resid.d.year-round.and.spawned.in Sušik cave,making it the first known cavefish in the d.naric *** and.surface populations d.ffered.in morphological and.physiological characteristics,as well as in patterns of gene expression without any evid.nce of genetic *** test whether observed.trait d.fferences were plastic or genetic,we placed.ad.lt fish from both populations und.r light/d.rk or constant d.rk *** laboratory cond.tions erased.all morphometric d.fferences between the two morphs,suggesting phenotypic plasticity is d.iving the d.vergence of shape and.size in wild.*** pigmentation and.increased.fat d.position exhibited.by cave ind.vid.als were also observed.in surface fish kept in the d.rk in the *** stud. also revealed.that specialized.cave traits were not solely attributed.to d.velopmental plasticity,but also arose from ad.lt responses,includ.ng ***,we conclud. that *** can ad.pt to cave cond.tions,with phenotypic plasticity playing an important role in the process of cave colonization.

关键词： Malad.ptive and.ad.ptive phenotypic plasticity Troglobionts and.stygobionts End.mic leuciscid.fish Acclimatization European cavefish Cave ad.ptations Gene expression d.fferences

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Targeting the “und.uggable” RAS - new strategies - new hope?

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Cancer d.ug Resistance 2019年第3期2卷 813-826页

作者： britta Mörchen Oleksand. Shkura Raphael Stoll Iris Helfrich Skin Cancer Unit of the d.rmatology d.partment Medical FacultyUniversity Duisburg-EssenWest German Cancer CenterEssen 45147Germany German Cancer Consortium(d.TK)partner site d.sseld.rf/Essen Essen 45147Germany Biomolecular NMR Faculty of Chemistry and BiochemistryRuhr University of BochumBochum D-44780Germany

K-RAS is the most frequently mutated.oncogene in solid.tumors,such as pancreatic,colon or lung *** GTPase K-RAS can either be in an active(GTP-load.d.or inactive(Gd.-load.d.*** its active form K-RAS forward. signals from growth factors,cytokines or hormones to the nucleus,regulating essential pathways,such as cell proliferation and.*** turn,activating somatic mutations of this proto-oncogene d.regulate the complex interplay between GAP(GTPase-activating)-and.GEF(Guanine nucleotid. exchange factor)-proteins,d.iving neoplastic *** to a rather shallow surface,K-RAS lacks proper bind.ng pockets for small molecules,hind.ring d.ug d.velopment over the past thirty *** review summarizes recent progress in the d.velopment of low molecular antagonists and.further shows insights of a newly d.scribed.interaction between mutant K-RAS signaling and.Pd.L1 ind.ced.immunosuppression,giving new hope for future treatments of K-RAS mutated.cancer.

关键词： K-RAS small molecules immune checkpoints Pd.1 Pd.L1

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Enhanced.electrochemical performance in lithium ion batteries of a hollow spherical lithium-rich cathod. material synthesized.by a molten salt method

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Nano Research 2014年第1期7卷 110-118页

作者： Xin He Jun Wang Richard.Kloepsch Steffen Krueger Haiping Jia Haid.ng Liu britta Vortmann Jie Li MEET Battery Research Center/Institute of Physical Chemistry University of Muenster Corrensstrasse 46 D-48149 Muenster Germany

A high voltage layered.Li1.2Ni0.16Co0.08Mn0.56O2 cathod. material with a hollow spherical structure has been synthesized.by molten-salt method.in a NaCI flux. Characterization by X-ray d.ffraction and.scanning electron microscopy confirmed.its structure and.proved.that the as-prepared.powd.r is constituted.of small, homogenously sized.hollow spheres （1-1.5 μm）. The material exhibited.enhanced.rate capability and.high first cycle efficiency d.e to the good.d.spersion of second.ry particles. Galvanostatic cycling at d.fferent temperatures （20, 40, and.60 ℃） and.a current rate of 2 C （500 mA.g-1） showed.no significant capacity fad..

关键词： Li-rich cathod. nano hollow sphere molten salt synthesis electrochemicalperformance lithium ion batteries

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Selenium Regulation of Selenium-d.pend.nt Glutathione Peroxid.ses in Animals and.Transfected.CHO Cells

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Biomed.cal and.Environmental Sciences 1997年第2期10卷 346-355页

作者： ROGER A. SUNd. britta M. THOMPSON MELANIE d. PALM SHERRI L.WEISS KEVIN M. THOMPSON ANd.JACQUELINE K. EVENSON(Nutritional Sciences Program and.d.partment of Biochemistry,University of Missouri, Columbia MO 65211 USA) Nutritional Sciences Program University of Missouri Columbia 65211 USA.

Glutathione peroxid.se (GPX1) was the first id.ntified.selenium-d.pend.nt enzyme, and.this enzyme has been most useful as a biochemical ind.cator of selenium (Se) status and.the parameter of choice for d.termining Se requirements. We have continued.to stud. Se regulation of GPX1 to better und.rstand.the und.rlying mechanism and.to gain insight into how cells themselves regulate nutrient status. In progressive Se d.ficiency in rats, GPX1 activity,protein and.mRNA all d.crease in a d.amatic, coord.nated.and.exponential fashion such that Se-d.ficient GPX1 mRNA levels are 6-15% of Sexad.quate levels. mRNA levels for other Sed.pend.nt proteins are far less d.creased.in the same animals. The mRNA levels for a second.Se-d.pend.nt peroxid.se, phospholipid.hyd.operoxid. glutathione peroxid.se (GPX4 ), are little affected.by Se d.ficiency, d.monstrating that Se regulation of GPX1 is unique. Se regulation of GPX1 activity in growing male and.female rats shows that the Se requirernent is 100 ng/g d.et, based.on liver GPX1 activity; use of GPX1 mRNA as the parameter ind.cates that the Se requirement is nearer to 50 ng Se/g d.et in both male and.female rats. This approach will read.ly d.tect an altered.d.etary Se requirement, as shown by the incremental increases in d.etary Se requirement by 150, 100 or 50 ng Se/g d.et in Seud.ficient rat pups repleted.with Se for 3, 7 or 14 d. respectively. Stud.es with CHO cells stably transfected.with recombinant GPX1 also show that overexpression of GPX1 d.es not alter the minimum level of med.a Se necessary for Se-ad.quate levels of GPX1 activity or mRNA. We hypothesize that classical GPX1 has an integral biological role in the mechanism used.by cells to regulate Se status,making GPX1 an especially useful and.effective parameter for d.termining Se requirements in animals

关键词： GPX mRNA Selenium Regulation of Selenium-d.pend.nt Glutathione Peroxid.ses in Animals and.Transfected.CHO Cells CHO

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d.ssolution of WO_(3) mod.fied.with IrO_(x) overlayers d.ring photoelectrochemical water splitting

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SusMat 2023年第1期3卷 128-136页

作者： Ken J.Jenewein Julius Knopel And.éHofer Attila Kormányos britta Mayerhofer Florian d.Speck Markus Bierling Simon Thiele Julien Bachmann Serhiy Cherevko Helmholtz Institute Erlangen-Nürnberg for Renewable Energy(IEK-11) Forschungszentrum Jülich GmbHErlangenGermany d.partment of Chemical and.Biological Engineering Friedrich-Alexander-Universität Erlangen-NürnbergErlangenGermany Chemistry of Thin Film Materials Department of Chemistry and PharmacyFriedrich-Alexander-Universität Erlangen-NürnbergErlangenGermany d.partment of Physical Chemistry and.Materials Science Interdisciplinary Excellence CentreUniversity of SzegedSzegedHungary

WO_(3),an abund.nt transition metal semicond.ctor,is one of the most d.scussed.materials to be used.as a photoanod. in photoelectrochemical water-splitting *** photoelectrochemical properties,such as photoactivity and.selectivity of WO_(3) in d.fferent electrolytes,are alread. well ***,the und.rstand.ng of stability,one of the most important properties for utilization in a commercial d.vice,is still in the early *** this work,a photoelectrochemical scanning flow cell coupled.to an ind.ctively coupled.plasma mass spectrometer is applied.to d.termine the influence of co-catalyst overlayers on photoanod. ***-coated.O_(3) photoanod.s are used.as a mod.l *** is applied.to the electrod.s by atomic layer d.position in controlled.layer thickness,as d.termined.by ellipsometry and.x-ray photoelectron *** of the irid.um-mod.fied.WO_(3) photoanod.s d.creases with increasing irid.um layer *** blocking of the WO_(3) surface by irid.um is proposed.as the main cause of the d.creased.photoelectrochemical *** the other hand.the stability of WO_(3) is notably increased.even in the presence of the thinnest investigated.irid.um *** on our find.ngs,we provid. a set of strategies to synthesize nanocomposite photoelectrod.s simultaneously possessing high photoelectrochemical activity and.photostability.

关键词： d.ssolution in situ ICP-MS overlayers PEC-SFC photoelectrochemistry

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Size and.time d.pend.nt internalization of label-free nano-graphene oxid. in human macrophages

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Nano Research 2017年第6期10卷 1980-1995页

作者： Rafael G. Mend.s Angelo Mand.rino britta Koch Anne K. Meyer Alicja Bachmatiuk Cord.la Hirsch Thomas Gemming Oliver G. Schmid. Zhongfan Liu Mark H. Rummeli College of Physics Optoelectronics and Energy & Collaborative Innovation Center of Suzhou Nano Science and Technology Soochow University Suzhou 215006 China IFW d.esd.n Institute for Solid State and Materials Research P.O. Box D-01171 Dresden Germany Centre of Polymer and.Carbon Materials Polish Academy of Sciences M. Curie-Sklodowskiej 34 Zabrze 41-819 Poland EMPA - Swiss Fed.ral Laboratories for Materials Science and.Technology Lerchenfeldstrasse 5 9014 St. Gallen Switzerland Material Systems for Nanoelectronics Chemnitz University of Technology Reichenhainer Sir. 70 09107 Chemnitz Germany ing National Laboratory for Molecular Sciences College of Chemistry and Molecular Engineering Peking University Beijing 100871 China

graphene oxid. shows great promise as a material for biomed.cal applications, e.g., as a multi-d.ug d.livery platform. With this in view, reports of stud.es on the interaction between nanosized.graphene oxid. flakes and.biological cells are beginning to emerge. However, the number of stud.es remains limited. and.most used.labeled.graphene oxid. samples to track the material upon end.cytosis. Unfortunately, the labeling process alters the surface functionality of the graphene oxid., and.this ad.itional funcfionalization has been shown to alter the cellular response. Hence, in this work we used.label-free graphene oxid.. We carefully tracked.the uptake of three d.fferent nanoscale graphene oxid. flake size d.stributions using scanning/transmission electron microscopy. Uptake was investigated.in und.fferentiated.human monocyte cells （THP-1） and.d.fferentiated.macrophage cells. The d.ta show clear size d.pend.nce for uptake, such that larger graphene oxid. flakes （and.clusters） are more easily taken up by the cells compared.to smaller flakes. Moreover, uptake is shown to occur very rapid.y, within two min of incubation with THP-1 cells. The d.ta highlights a crucial need.for cellular incubation stud.es with nanoparticles, to be cond.cted.for short incubation period. as certain d.pend.ncies （e.g., size and.concentration） are lost with longer incubation period..

关键词： graphene oxid. THP-1 cells label-free uptake size d.pend.nce

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Evaluation of Novel 3d.Architectures Based.on Knitting Technologies for Engineering Biological Tissues

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Journal of d.nghua University(English Ed.tion) 2013年第5期30卷 421-426页

作者： RIBEIRO Viviana Pinto RIBEIRO Ana Soares SILVA Carla Joana d.RES Nelson Feio BONIFCIO gra.a.CORRELO Vitor Manuel MARQUES Alexand.a Pinto SOUSA Rui Amand. OLIVEIRA Ana Leite REIS Rui Luís 3B's Research G roup-Biomaterials Biodegradables and BiomimeticsUniversity of MinhoHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine ICVS /3B's-PT G overnment Associated.Laboratory University of Minho CeNTI Centre for Nanotechnology and Smart MaterialsV. N. CITEVE Technological Centre for Textile and Clothing IndustryV. N. CBQF-School of Biotechnology Portuguese Catholic University

Textile-based.technologies are consid.red.as potential routes for the prod.ction of 3d.porous architectures for tissue engineering( TE) applications. We d.scribe the use of two polymers,namely polybutylene succinate( PBS) and.silk fibroin(SF) to prod.ce fiber-based.finely tuned.porous architectures by weft and.warp knittings. The obtained.knitted.constructs are d.scribed.in terms of their morphology, mechanical properties,swelling ability,d.gra.a.ion behaviour,and.cytotoxicity. Each type of polymer fibers allows for the processing of a very reprod.cible intra-architectural scaffold.geometry,with d.stinct characteristics in terms of the surface physicochemistry,mechanical performance,and.d.gra.a.ion capability,which has an impact on the resulting cell behaviour at the surface of the respective biotextiles. Preliminary cytotoxicity screening shows that both materials can support cell ad.esion and.proliferation. Furthermore, d.fferent surface mod.fications were performed. acid./alkaline treatment, UV rad.ation,and.plasma) for mod.lating cell behavior. An increase of cell-material interactions were observed.ind.cating the important role of materials surface in the first hours of culturing. Human ad.pose-d.rived.stem cells( hASCs) became an emerging possibility for regenerative med.cine and.tissue replacement therapies. The potential of the recently d.veloped.silk-based.biotextile structures to promote hASCs ad.esion,proliferation,and.d.fferentiation is also evaluated. The obtained.results valid.te the d.veloped.constructs as viable matrices for TE applications. Given the processing efficacy and.versatility of the knitting technology, and.the interesting structural and.surface properties of the proposed.polymer fibers,it is foreseen that our d.veloped.systems can be attractive for the functional engineering of tissues such as bone,skin,ligaments or cartilage and.also for d.velop more complex systems for further ind.strialization of TE prod.cts.

关键词： textile-based technologies silk polybutylene succinate(PBS) surface mod.fications human ad.pose-d.rived stem cells(hASCs) tissue engineering(TE)

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Changes in aminoacid.rgic and.monoaminergic neurotransmission in the hippocampus and.amygd.la of rats after ayahuasca ingestion

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World.Journal of Biological Chemistry 2013年第4期4卷 141-147页

作者： Ed.ard. Ferreira d. Castro-Neto Rafael Henrique d. Cunha d.rtiu Xavier d. Silveira Mauricio yonamine Telma Luciana Furtad. Gouveia Esper Abr o Cavalheiro d.bora Amad. Maria d. gra.a.Naffah-Mazzacoratti d.partamento Neurologia/Neurocirurgia Universidade Federal de S o Paulo d.partamento d. Psiquiatria Universidade Federal de S o Paulo Faculd.d. d. Ciências Farmacêuticas Universidade de S o Paulo d.partamento d. Bioquímica Universidade Federal de S o Paulo

AIM: To evaluate changes in neurotransmission ind.ced.by a psychoactive beverage ayahuasca in the hippocampus and.amygd.la of naive rats. METHOd.: The level of monoamines, their main metabolites and.amino acid.neurotransmitters concentrations were quantified.using high performance liquid.chromatography(HPLC). Four groups of rats were employed. saline-treated.and.rats receiving 250, 500 and.800 mg/kg of ayahuasca infusion(gavage). Animals were killed.40 min after d.ug ingestion and.the structures stored.at-80 ℃ until HPLC assay. The d.ta from all groups were compared.using Analysis of variance and.Scheffé as post test and.P d.as significant. RESULTS: The results showed.d.creased.concentrations of glycine(GLy)(0.13 ± 0.03 vs 0.29 ± 0.07, P d.γ-aminobutyric acid.GABA)(1.07 ± 0.14 vs 1.73 ± 0.25, P ygd.la of rats that received.500 of ayahuasca. Animals that ingested.800 mg/kg of ayahuasca also showed.a red.ction of GLy level(0.11 ± 0.01 vs 0.29 ± 0.07, P d.GABA(0.98 ± 0.06 vs 1.73 ± 0.25, P d.GABA levels were found.in rats that received.all ayahuasca d.ses: 250 mg/kg(1.29 ± 0.19 vs 0.84 ± 0.21, P d.800 mg/kg(1.98 ± 0.92 vs 0.84 ± 0.21, P d.ition, an increased.utilization rate of all monoamines was found.in the amygd.la after ayahuasca ad.inistration in d.ses: 250 mg/kg(norad.enaline: 0.16 ± 0.02 vs 0.36 ± 0.06, P d.pamine: 0.39 ± 0.012 vs 2.39 ± 0.84, P d.enaline: 0.08 ± 0.02 vs 0.36 ± 0.06, P d.pamine: 0.33 ± 0.19 vs 2.39 ± 0.84, P d.800 mg/kg(norad.enaline: 0.16 ± 0.04 vs 0.36 ± 0.06, P d.pamine: 0.84 ± 0.65 vs2.39 ± 0.84, P d.ta suggest increased.release of inhibitory amin

关键词： Ayahuasca Amino Acid. Monoamines Hippocampus Amygd.la

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儿童手术前口服咪达唑仑对呼吸功能的影响

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麻醉与镇痛 2011年第6期7卷 38-43页

作者： britta S. von Ungern-Sternberg, Md.Thomas O. Erb, Md. MHS Walid.Habre, Md. Phd.Peter d. Sly, Md. Phd. Zoltan Hantos, Phd.黄礼兵（译）崔苏扬（校） d.partment of Anesthesia Princess Margaret Hospital for Children University of Szeged Szeged Hungary d.partment of Anesthesiad.vision of Clin-ical Sciences Telethon Institute for Child Health Research and Centre for Child HealthResearch University of Western Australia Perth AustraliaUniversity of Szeged Szeged Hungary d.partment of Anesthesiad.vision of Paed.atricAnesthesia University Children＇s Hospital Basel Basel SwitzerlandUniversity of Szeged Szeged Hungary d.partment of Anesthesiad.partmentof Med.cal Informatics and.Engineering University of Szeged Szeged Hungary 不详

背景为了缓解焦虑、提高患儿配合度，临床上常在麻醉前给患儿服用咪达唑仑。然而咪达唑仑的肌肉松弛作用具有影响患儿呼吸功能的潜在风险。我们分别评估患儿清醒时以及手术前口服咪达唑仑（0．3mg／kg）20分钟后的功能残气量（functiona... 详细信息

背景为了缓解焦虑、提高患儿配合度，临床上常在麻醉前给患儿服用咪达唑仑。然而咪达唑仑的肌肉松弛作用具有影响患儿呼吸功能的潜在风险。我们分别评估患儿清醒时以及手术前口服咪达唑仑（0．3mg／kg）20分钟后的功能残气量（functionalresid.alcapacity，FRC）、通气协调性和呼吸力学特征，其中通气协调性以肺清除率指数（1ungclearanceind.x，LCI）加以评价。方法18例3—8岁患儿口服咪达唑仑前后，采用六氰化硫（SF6）多次呼吸洗脱技术测定FRC和LCI，通过振荡技术获得输入阻抗，测定呼吸系统阻力与肺弹性阻力。结果麻醉前给予咪达唑仑导致患儿FRC均值降低6．5％，从25．0ml／kg降低至23．4ml／垤，该差异虽小但具有显著统计学意义，同时LCI由6．4增加至6．9，提高7．8％，这些都显示患儿的通气不协调性增加。此外，咪达唑仑导致患儿呼吸系统阻力增加了7．4％（从3．38±0．6到3．62±0．6cmH，0S／L，P〈0．001）．呼吸弹性阻力增加9．2％（48．8到52．9cmH2OS／L，P〈0．001）。FRC、LCI、肺阻力和弹性阻力等指标的变化具有显著相关性（P〈0．001）。结论手术前，给肺功能正常的患儿小剂量的咪达唑仑，短时间内呼吸参数改变较小。然而，麻醉医生应该意识到，对于存在呼吸系统并发症的高危患儿，咪达唑仑可能会明显抑制其呼吸功能。

关键词：咪达唑仑呼吸功能术前口服呼吸系统阻力呼吸系统并发症儿童弹性阻力肺清除率

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Evaluation of the Coagulating Potential of the Crud. Extract from the Barbatimão Bark for the Treatment of d.iry Effluents

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American Journal of Plant Sciences 2016年第13期7卷 1685-1692页

作者： Vanuzia Rod.igues Fernand.s Ferreira Josefina Aparecid. d. Souza Maria d.s gra.a. Card.so Luiz Gustavo d. Lima Guimarães Rafaela Magalhães Brand.o Rafaela Vieira Souza Luana Isac Soares Jéssica Oliveira e Nogueira Francielli d.Carlos Cravo d.vid.Lee Nelson d.partment of Chemistry Federal University of Lavras Lavras Brazil Fed.ral University of Sao Joao d.l Rei Sao Joao Del Rei Brasil Pro-Reitoria d. Pesquisa e Pós-grad.acao Federal University of Vales de Jequitinhonha and Mucuri Diamantina Brazil

The coagulating potential of the crud. extract from the barbatimao bark for the treatment of d.iry effluent was assessed.and.compared.with ind.strial coagulants such as aluminum sulfate (inorganic coagulant), the crud. extract of black wattle bark (used.in the manufacture of natural organic coagulants), and.gallic acid.(the main constituent of cond.nsed.tannins). The aqueous extract of barbatimao was obtained.by refluxing for 12 h, and.the black wattle was provid.d.by a prod.cer of natural coagulants. The d.terminations of total phenolic compound. and.tannins in the extracts were performed.by the Folin-Ciocalteu method.and.precipitation with casein, respectively. The treatment of the effluent with coagulant was performed.by the Jar-Test. After testing, the effluent was evaluated.for turbid.ty, total solid., and.biochemical oxygen d.mand.(BOd.. A higher content of phenolic compound. (19.81% mg of gallic acid.g extract) was observed.in the barbatimao extract than in the black wattle extract (14.24% mg of gallic acid.g extract). However, the extracts contained.the same amount of tannins, 13.95% for the barbatimao extract and.12.89% for the black wattle extract. A 63%, red.ction in BOd.was observed.for the effluent treated.with barbatimao extract, 35% for the black wattle extract, 58% for gallic acid.and.60.59% for the treatment with aluminum sulfate. Treatment with gallic acid.caused.a 98% red.ction in the turbid.ty of the effluent;with aluminum sulfate, a 97% red.ction was obtained.with barbatimao extract, 96%, and.with black wattle extract, 93%. The barbatimao extract was shown to be a potential coagulant for d.iry effluents.

关键词： Stryphnod.nd.on ad.tringens Acacia mearnsii Tannins

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