检索结果-南通市图书馆

Characterization of genetic humanized mice with transgenic HLA DP401 or DRA but deficient in endogenous murine MHC classⅡgenes upon Staphylococcus aureus pneumonia

引用

Animal Models and Experimental Medicine 2023年第6期6卷 585-597页

作者： Feng Li Bowen Niu Lingling Liu Mengmin Zhu Hua Yang boyin qin Xiuhua Peng Lixiang Chen Chunhua Xu Xiaohui Zhou Department of Laboratory Animal Science Shanghai Public Health Clinical CenterShanghaiChina

Background:Staphylococcus aureus can cause serious infections by secreting many superantigen exotoxins in“carrier”or“pathogenic”*** DQ and HLA DR humanized mice have been used as a small animal model to study the role of two molecules during *** ***,the contribution of HLA DP to *** infection is unknown ***:In this study,we have produced HLA DP401 and HLA DRA0101 humanized mice by microinjection of C57BL/6J ***-floxed IAβ+/-mice were crossbred with Ella-Cre and further crossbred with HLA DP401 or HLA-DRA0101 humanized *** several rounds of traditional crossbreeding,we finally obtained HLA DP401-IAβ-/-and HLA DRA-IAβ-/-humanized mice,in which human DP401 or DRA0101 molecule was introduced into IAβ-/-mice deficient in endogenous murine MHC classⅡmolecules.A transnasal infection murine model of *** pneumonia was induced in the humanized mice by administering 2×108CFU of *** Newman dropwise into the nasal *** immune responses and histopathology changes were further assessed in lungs in these infected ***:We evaluated the local and systemic effects of *** delivered intranasally in HLA DP401-IAβ-/-and HLA DRA-IAβ-/-transgenic *** Newman infection significantly increased the m RNA level of IL 12p40 in lungs in humanized *** increase in IFN-γand IL-6 protein was observed in HLA DRA-IAβ-/-*** observed a declining trend in the percentage of F4/80+macrophages in lungs in HLA DP401-IAβ-/-mice and a decreasing ratio of CD4+to CD8+T cells in lungs in IAβ-/-mice and HLA DP401-IAβ-/-mice.A decreasing ratio of Vβ3+to Vβ8+T cells was also found in the lymph node of IAβ-/-mice and HLA DP401-IAβ-/-*** Newman infection resulted in a weaker pathological injury in lungs in IAβ-/-genetic background ***:These humanized mice will be an invaluable mouse model to resolve the pathological mechanism of *** pneumonia and study what role DP molecule plays in *** in

关键词： HLA DP401 HLA-DRA humanized mice MHC II Staphylococcus aureus pneumonia transgene

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of fluoride and cadmium co-exposure on bone in male rats

Effects of fluoride and cadmium co-exposure on bone in male ...

引用

中华医学会第五次中青年骨质疏松和骨矿盐疾病学术会议

作者： Xiao Chen boyin qin Xufang Li Taiyi Jin Wenjiang Zhou Guoying Zhu 复旦大学

Objective Although cadmium and fluoride may both have adverse effects on bone,most studies focus on a single *** this study,we investigated the effects of cadmium and fluoride on bone at a relative low level. Material...

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

The role of RIP3 in the induction of influenza viral antigen specific CD8+T cell responses in C57BL/6 mice upon H1N1influenza virus infection

The role of RIP3 in the induction of influenza viral antigen...

引用

第十四届全国免疫学学术大会

作者： boyin qin Chao Wang Lixiang Chen Yang Liu Xiaonan Ren Bin Wu Shun Li Xiaohui Zhou Shanghai public health clinical center

Objective:The purpose of this study was to explore whether RIP3 is involved in the induction and function of antigen-specific CD8T cells and memory CD8T cells in mice infected with influenza A virus ***:RIP3 and WT mice were infected through intranasal with influenza A H1 N1 PR8 virus(0.5 x LD50),*** spleen and peripheral blood of mice were dissected and collected in the post-infection effect stage(7 days after infection),memory formation stage β7 days after infection) and memory response stage β days after reinfection).The number and proportion of virus-specific effector CD8+T cells were detected by flow cytometry,and the expression of cytokines IFN-γ,TNF-α,IL-2 and GranzymeB were detected by flow *** the same time,the expression of immune checkpoint molecules PD-1,LAG-3,Tim-3 and CTLA-4 of CD8CD44 T cell subsets in mouse spleen was *** proportion of CD8T cells differentiated into TCM and TEM/TEff in the memory response phase was also ***:In the effect stage,there were few influenza antigen-specific CD8T cells in the spleen of RIP3 infected mice,and the expression levels of effector cytokines IFN-γ,TNF-α,IL-2 and GranzymeB on CD8+T cells were significantly lower than those in WT *** the same time,the immune checkpoint molecules LAG-3 and Tim-3 expressed by CD8+T cells in RIP3 infected group were significantly lower than those in WT *** the memory stage,the number of antigen-specific CD8T cells in peripheral blood of RIP3 mice was significantly lower than that of WT *** the stage of memory response,the number of antigen-specific CD8T cells produced by RIP3 mice and the ability of CD8T cells to secrete IFN-y were significantly lower than those of WT *** the phenotypes of activated memory CD8+T cells,the proportion of TEM in WT mice was significantly higher than that in RIP3 mice,while the proportion of TCM in WT mice was significantly lower than that in RIP3 ***:This study suggests t

关键词： Receptor-interacting protein 3(RIP3) H1N1 influenza virus antigen specific CD8+T cells

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Constraints on the primordial curvature power spectrum by pulsar timing array data:a polynomial parameterization approach

引用

Communications in Theoretical Physics 2024年第1期76卷 96-104页

作者： qin Fei School of Mathematics and Physics Hubei Polytechnic UniversityHuangshi 435003China

The recent stochastic signal observed jointly by NANOGrav,parkes pulsar timing array,European pulsar timing array,and Chinese pulsar timing array can be accounted for by scalarinduced gravitational waves(SIGWs).The source of the SIGWs is from the primordial curvature perturbations,and the main contribution to the SIGWs is from the peak of the primordial curvature power *** effectively model this peak,we apply the Taylor expansion to parameterize *** the Taylor expansion parameterization,we apply Bayesian methods to constrain the primordial curvature power spectrum based on the NANOGrav 15 year data *** constraint on the primordial curvature power spectrum possesses a degree of generality,as the Taylor expansion can effectively approximate a wide range of function profiles.

关键词： pulsar timing array scalar-induced gravitational waves inflation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Numerical study on the blocking effect of skin on Flash-Ball Impact and damage assessment

引用

Defence Technology（防务技术） 2024年第3期33卷 532-545页

作者： Dihua Ouyang Chunwei qin Xiaowen qin Qiantao Zhang Xingyu Liu Xi'an University of Architecture and Technology Xi'an 710055China Analysis and Testing Center Xi'an University of Architecture and TechnologyXi'an 710055China Engineering University of PAP Xi'an 710086China

To explore the response law of non-lethal large-size kinetic energy projectiles to blunt attack on skin tissue,and to evaluate the skin injury characteristics of the attacked personnel and the use safety of kinetic energy *** on the LS-DYNA simulation software,a three-layer skin simulation model and a Flash-Ball rubber bullet model are established,and the force-time and deformation-time biomechanical corridors of the Flash-Ball rubber bullet impacting human skin tissue are *** corridor curve and the energy transfer and diffusion are analyzed and *** safety evaluation of the damage caused by the rubber bullet shooting a human body at different distances is carried out using the empirical formula of the penetration ***,the safe shooting distance is *** results show that the model used in the simulation has a good correlation with the experimental data,its biomechanical corridor characteristics are different from those of conventional vehicle impact and smallsize projectile response *** energy transfer and action time of medium and low-speed impact may cause greater *** fat layer is the largest energy absorption *** minimum safe shooting distance to ensure skin tissue from penetrating damage is 15.8 m,and the limit specific kinetic energy of skin damage is 7.88 J/cm^(2).This study can be extended to the study of biomechanical response law and safety evaluation under the impact of the same type of large kinetic energy projectile,which provides an important theoretical reference for the police to use large kinetic energy projectiles to conduct safe shooting in peacekeeping operations.

关键词： KIPs Blunt strike Biomechanical response Damage analysis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

An Icy Worlds life detection strategy based on Exo-AUV

引用

Science China Earth Sciences 2024年第11期67卷 3515-3535页

作者： Bin WANG Hongde qin National Key Laboratory of Autonomous Marine Vehicle Technology Harbin Engineering University Shipbuilding Engineering College Harbin Engineering University

In the solar system, Icy Worlds such as Europa and Enceladus hold great potential for extraterrestrial life and may provide humanity an answer, within this century, to the age-old question of life beyond Earth. Exo-AUV technology shows promise in life detection in the icy shell, at the ice-water interface and on the seafloor of exo-ocean. Space agencies, including NASA and DLR, are enthusiastic about deploying Exo-AUVs to explore life in these regions. However, the where and how to find life, the technologies to be utilized and the goals to be achieved are crucial aspects for future Exo-AUV life detection missions on Icy Worlds. This study delves into a hypothetical mission of life detection on Europa, discussing science goals,detectable objects, potential regions and biogenic analysis for Icy Worlds. It proposes a life detection strategy for Icy Worlds based on Exo-AUVs, presents key contextual elements for Exo-AUV operations, outlines technological requirements for hull,payloads and autonomy, introduces the current state of Exo-AUV research and addresses existing challenges. This study also suggests a roadmap for conceptual development of Exo-AUV and a Concept of Operations for Multiple Exo-AUV System(ConOps for MEAS). This system aims to assist planetary scientists and astrobiologists in exploring Icy Worlds, identifying robust biosignatures and potentially discovering extant organisms, even prebiotic chemical systems.

关键词： Icy Worlds Europa Life detection Biological potential Exo-AUV

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Limit state analysis of rigid retaining structures against seismically induced passive failure in heterogeneous soils

引用

Journal of Rock Mechanics and Geotechnical Engineering 2024年第3期16卷 1095-1105页

作者： Jianfeng Zhou Changbing qin College of Civil Engineering Huaqiao UniversityXiamen362021China School of Civil Engineering Chongqing UniversityChongqing400045China Key Laboratory of New Technology for Construction of Cities in Mountain Area Chongqing UniversityChongqing400045China National Joint Engineering Research Center of Geohazards Prevention in the Reservoir Areas Chongqing UniversityChongqing400045China

Soils are not necessarily uniform and may present linearly varied or layered characteristics,for example the backfilled soils behind rigid retaining *** the presence of large lateral thrust imposed by arch bridge,passive soil failure is possible.A reliable prediction of passive earth pressure for the design of such wall is challenging in complicated soil strata,when adopting the conventional limit analysis *** order to overcome the challenge for generating a kinematically admissible velocity field and a statically allowable stress field,finite element method is incorporated into limit analysis,forming finiteelement upper-bound(FEUB)and finite-element lower-bound(FELB)***-static,original and modified pseudo-dynamic approaches are adopted to represent seismic acceleration *** generating feasible velocity and stress fields within discretized elements based on specific criteria,FEUB and FELB formulations of seismic passive earth pressure(coefficient K_(P))can be derived from work rate balance equation and stress *** to an interior point algorithm,optimal upper and lower bound solutions are *** proposed FEUB and FELB procedures are well validated by limit equilibrium as well as lower-bound and kinematic *** studies are carried out to investigate the effects of influential factors on seismic K_(P).Notably,true solution of K_(P) is well estimated based on less than 5%difference between FEUB and FELB solutions under such complex scenarios.

关键词： Retaining wall Passive earth pressure Earthquakes Finite-element limit-analysis methods

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

AI for Supporting the Freedom of Drawing

引用

Machine Intelligence Research 2024年第1期21卷 63-88页

作者： Xiaohua Sun Juexiao qin College of Design and Innovation Tongji UniversityShanghai 200082China

Artificial intelligence(AI)has recently been developing rapidly in image processing and *** is not only starting to take over repetitive and tedious tasks but is also involved in creative *** is one of the areas with the greatest potential for collaboration between humans and *** is an important approach to express various information,but due to the lack of appropriate knowledge and skills,ordinary people without long-time training are unable to draw *** there have been various attempts at human-AI collaboration in drawing,it is difficult for researchers to consider the wide variety of specific problems and develop univer-sal methods due to the openness,improvisation,and individuality of *** this paper,we first analysed the contents of drawing and the general creation process in ***,we have described a mechanism for using AI to enable people to regain the freedom of drawing ***,we have developed a framework that describes methods for analysing specific problems and quickly finding solutions by building connections between the influencing factors in drawing,the demands of humans,and possible implementa-tion *** framework also reveals a broad scope of possibilities for applying AI to support people in drawing.

关键词： Intention understanding drawing support drawing art human-artificial intelligence(AI)collaboration

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Roles of Upper-Level Descending Inflow in Moat Development in Simulated Tropical Cyclones with Secondary Eyewall Formation

引用

Advances in Atmospheric Sciences 2024年第6期41卷 1100-1114页

作者： Nannan qin Liguang WU Nanjing Joint Institute for Atmospheric Sciences Nanjing 210041China Shanghai Typhoon Institute China Meteorological AdministrationShanghai 200030China Department of Atmospheric and Oceanic Sciences and Institute of Atmospheric Sciences Fudan UniversityShanghai 200438China Innovation Center of Ocean and Atmosphere System Zhuhai Fudan Innovation Research InstituteZhuhai 518057China

This study investigated the effects of upper-level descending inflow(ULDI)associated with inner-eyewall convection on the formation of the moat in tropical cyclones(TCs)with secondary eyewall formation(SEF).In our numerical experiments,a clear moat with SEF occurred in TCs with a significant ULDI,while no SEF occurred in TCs without a significant *** eyewall convection developed more vigorously in the control run.A ULDI occurred outside the inner-eyewall convection,where it was symmetrically *** ULDI was initially triggered by the diabatic warming released by the inner eyewall and later enhanced by the cooling below the anvil *** ULDI penetrated the outer edge of the inner eyewall with relatively dry air and prevented excessive solid-phase hydrometeors from being advected further *** produced extensive sublimation cooling of falling hydrometeors between the eyewall and the outer *** sublimation cooling resulted in negative buoyancy and further induced strong subsidence between the eyewall and the outer *** a result,a clear moat was *** of the moat in the ongoing SEF prevented the outer rainband from moving farther inward,helping the outer rainband to symmetrize into an outer *** the sensitivity experiment,no significant ULDI formed since the eyewall convection was weaker,and the eyewall anvil developed relatively lower,meaning the formation of a moat and thus an outer eyewall was less *** study suggests that a better-represented simulation of inner-eyewall convective structures and distribution of the solid-phase hydrometeors is important to the prediction of SEF.

关键词： tropical cyclone secondary eyewall formation upper-level descending inflow eyewall convection

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The Coarse l^(p)-Novikov Conjecture and Banach Spaces with Property(H)

引用

Chinese Annals of Mathematics,Series B 2024年第2期45卷 193-220页

作者： Huan WANG qin WANG Research Center for Operator Algebras School of Mathematical SciencesEast China Normal UniversityShanghai 200241China Key Laboratory of MEA Ministry of Educationand Shanghai Key Laboratory of PMMPEast China Normal UniversityShanghai 200241China

In this paper,for 1

关键词： The coarse l^(p)-Novikov conjecture Banach spaces with Property(H) Coarse geometry K-Theory

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：