The role of RIP3 in the induction of influenza viral antigen specific CD8+T cell responses in C57BL/6 mice upon H1N1influenza virus infection
作者单位：Shanghai public health clinical center
会议名称：《第十四届全国免疫学学术大会》
会议日期：2021年
学科分类：1001[医学-基础医学(可授医学、理学学位)] 100102[医学-免疫学] 10[医学]
关 键 词：Receptor-interacting protein 3(RIP3) H1N1 influenza virus antigen specific CD8+T cells
摘 要：Objective:The purpose of this study was to explore whether RIP3 is involved in the induction and function of antigen-specific CD8T cells and memory CD8T cells in mice infected with influenza A virus ***:RIP3 and WT mice were infected through intranasal with influenza A H1 N1 PR8 virus(0.5 x LD50),*** spleen and peripheral blood of mice were dissected and collected in the post-infection effect stage(7 days after infection),memory formation stage β7 days after infection) and memory response stage β days after reinfection).The number and proportion of virus-specific effector CD8+T cells were detected by flow cytometry,and the expression of cytokines IFN-γ,TNF-α,IL-2 and GranzymeB were detected by flow *** the same time,the expression of immune checkpoint molecules PD-1,LAG-3,Tim-3 and CTLA-4 of CD8CD44 T cell subsets in mouse spleen was *** proportion of CD8T cells differentiated into TCM and TEM/TEff in the memory response phase was also ***:In the effect stage,there were few influenza antigen-specific CD8T cells in the spleen of RIP3 infected mice,and the expression levels of effector cytokines IFN-γ,TNF-α,IL-2 and GranzymeB on CD8+T cells were significantly lower than those in WT *** the same time,the immune checkpoint molecules LAG-3 and Tim-3 expressed by CD8+T cells in RIP3 infected group were significantly lower than those in WT *** the memory stage,the number of antigen-specific CD8T cells in peripheral blood of RIP3 mice was significantly lower than that of WT *** the stage of memory response,the number of antigen-specific CD8T cells produced by RIP3 mice and the ability of CD8T cells to secrete IFN-y were significantly lower than those of WT *** the phenotypes of activated memory CD8+T cells,the proportion of TEM in WT mice was significantly higher than that in RIP3 mice,while the proportion of TCM in WT mice was significantly lower than that in RIP3 ***:This study suggests t