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In vitro biofilm forming capacity on abiotic contact surfaces by outbreak-associated Vibrio harveyi strains

Journal of Coastal Life Medicine 2014年第2期2卷 132-140页

作者： Pallaval Veera Bramha Chari Kuchipudi Viswadeepika bottu anand kumar Department of Biotechnology Krishna UniversityMachilipatnam-521001Andhra PradeshIndia

Objective:To evaluate the in vitro biofilm forming capacity on abiotic food contact surfaces by Vibrio harveyi(***)***:Thirty six Gram-negative *** strains were isolated from various street vended seafood outlets in a food processing line and evaluated for their ability to produce mucoid biofilms on food contact surfaces using a microplate *** characterization of mucoid biofilm producing *** strains were screened on Congo red agar,thiosulfate-citrate-bile salts-sucrose agar and tryptic soy agar,***:Only five *** strains(14%)were mucoid biofilm producers characterized by formation of black colonies,whereas the remaining 31 strains(86%)were not capable of producing biofilm characterized by formation of red colonies or pinkish-red colonies with darkening at the *** morphological,physiological and biochemical characteristics of these isolates were studied using standard *** identification was confirmed by polymerase chain reaction targeted to species-specific polymerase chain reaction primers VH-1 and VH-2 corresponding to variable regions of *** 16S rRNA *** the biofilm-forming strains showed resistance to at least three antimicrobial compounds *** strains isolated from various seafood were able to form biofilms of different capacity,and the strains VB267,VB238 and VB166 isolated from cat fish,shrimp and eel fish exhibited significantly greater biofilm forming ability compared to other ***:It can be concluded from the present study that the strain VB166 was able to better attach and form subsequent biofilms on glass and stainless steel compared to high density *** properties allow these bacteria to survive,proliferate and persist in street vended seafood outlets.

关键词： Mucoid biofilm Congo red agar Vibrio harveyi Polymerase chain reaction High density polyethylene

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