In vitro biofilm forming capacity on abiotic contact surfaces by outbreak-associated Vibrio harveyi strains
In vitro biofilm forming capacity on abiotic contact surfaces by outbreak-associated Vibrio harveyi strains作者机构：Department of BiotechnologyKrishna UniversityMachilipatnam-521001Andhra PradeshIndia
出 版 物：《Journal of Coastal Life Medicine》 (海岸生命医学杂志（英文版）)
年 卷 期：2014年第2卷第2期
页 面：132-140页
学科分类：0202[经济学-应用经济学] 02[经济学] 020205[经济学-产业经济学]
基 金：Supported by Rajiv Gandhi National Fellowship as Junior Research Fellowship from University Grants Commission New Delhi Govt.of India[Grant No:F.16-1723(SC)/2010(SA-III)]
主 题：Mucoid biofilm Congo red agar Vibrio harveyi Polymerase chain reaction High density polyethylene
摘 要：Objective:To evaluate the in vitro biofilm forming capacity on abiotic food contact surfaces by Vibrio harveyi(***)***:Thirty six Gram-negative *** strains were isolated from various street vended seafood outlets in a food processing line and evaluated for their ability to produce mucoid biofilms on food contact surfaces using a microplate *** characterization of mucoid biofilm producing *** strains were screened on Congo red agar,thiosulfate-citrate-bile salts-sucrose agar and tryptic soy agar,***:Only five *** strains(14%)were mucoid biofilm producers characterized by formation of black colonies,whereas the remaining 31 strains(86%)were not capable of producing biofilm characterized by formation of red colonies or pinkish-red colonies with darkening at the *** morphological,physiological and biochemical characteristics of these isolates were studied using standard *** identification was confirmed by polymerase chain reaction targeted to species-specific polymerase chain reaction primers VH-1 and VH-2 corresponding to variable regions of *** 16S rRNA *** the biofilm-forming strains showed resistance to at least three antimicrobial compounds *** strains isolated from various seafood were able to form biofilms of different capacity,and the strains VB267,VB238 and VB166 isolated from cat fish,shrimp and eel fish exhibited significantly greater biofilm forming ability compared to other ***:It can be concluded from the present study that the strain VB166 was able to better attach and form subsequent biofilms on glass and stainless steel compared to high density *** properties allow these bacteria to survive,proliferate and persist in street vended seafood outlets.
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