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Inhibition of candida adhesion to denture acrylic by Boesenbergia pandurata

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Asian Pacific Journal of Tropical Medicine 2010年第4期3卷 272-275页

作者： thaweboon Sroisiri thaweboon boonyanit Department of Microbiology Faculty of DentistryMahidol UniversityBangkok 10400Thailand

Objective:To investigate effect of Boesenbergia pandurata(***) rhizome extract on adhesion of Candida albicans(***) to acrylic ***:Transparent acrylic strips were prepared and divided into three groups with pretreatment by extract solution of B. pandurata rhizome at concentration of 25,50 and 100 mg/mL,*** washing, the strips were then inoculated with two strains of ***(ATCC13803 and the clinical isolate)(10~7 cells/mL).Normal saline solution and 0.2%chlorhexidine gluconate were used as negative and positive controls,*** the strips with modified Cram stain without *** yeast cells were direct counted under microscope(OlympusCX31, Japan) in 20 randomly selected fields on each *** statistical significance was calculated by Kruskal-Wallis and Mann-Whitney non-parametric tests at a significance level of P < ***:Pretreatment with *** extract significantly reduced the adhesion of both strains of *** to acrylic surfaces in a dose dependent ***: This observation indicates that *** extract has an inhibitory effect on the ability of C. albicans to adhere to denture acrylic and could be employed as an antifungal agent for preventing denture stomatitis.

关键词： Candida Adhesion Denture Boesenbergia pandurata

Effect of lead on IL-8 production and cell proliferation in human oral keratinocytes

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Asian Pacific Journal of Tropical Medicine 2010年第6期3卷 475-478页

作者： thaweboon Srosiri Poomsawat Sopee thaweboon boonyanit Department of Microbiology Faculty of DentistryMahidol University Yothi StreetBangkok 10400 Thailand Department of Oral Pathology Faculty of DentistryMahidol University Yothi StreetBangkok 10400 Thailand

Objective:To investigate the effect of lead on the production of IL-8 and cell proliferation in normal human oral keratinocytes（NHKs）.Methods:NHKs were prepared as outgrowths from normal human buccal *** cells were treated with three concentrations of lead glutamate(4.5×10-5M,4.5×10-6M and 4.5×10-7M).NHKs grown in glutamic acid were used as *** amounts of IL-8 secreted in the culture supernatants were evaluated at 12 and 24 h using enzyme-linked immunospecific assay（ELISA）.Cell proliferation was determined by the MTT colorimetric *** cultures were used for each experiment,and three independent experiments were *** of variance and Duncan’s multiple range tests were used for statistical ***:An elevation of IL-8 in culture supernatants of NHKs treated with lead at all concentrations at 12 and 24 h after exposure in a dose-dependent manner was revealed.A significant increase in cell numbers was observed only at 24 h exposed to 4.5×10- 5M lead ***:The capacity of NHKs,to secrete IL-8,enhanced by lead glutamate,is demonstrated *** of cell proliferation is revealed only after exposure to high lead *** elevation of secreted IL-8 is a probable initial sign for the acute inflammatory response and may be involved in the pathogenesis of lead stomatitis.

关键词： Cell proliferation Interleukin 8 Lead Oral keratinocytes Ulcerative stomatitis

Characterization of the salivary microbiome in healthy Thai children

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Asian Pacific Journal of Tropical Medicine 2019年第4期12卷 163-169页

作者： Izumi Mashima Citra F.Theodorea boonyanit thaweboon Sroisiri thaweboon Tippanart Vichayanrat Frank A.Scannapieco Futoshi Nakazawa Department of Microbiology School of Pharmacy Aichi Gakuin University Department of Oral Biology Faculty of Dentistry Universitas Indonesia Department of Oral Microbiology Faculty of Dentistry Mahidol University Department of Community Dentistry Faculty of Dentistry Mihidol University Department of Oral Biology School of Dental Medicine University at Buffalo the State University of New York Department of Microbiology School of Dentistry Health Sciences University of Hokkaido

Objective: To investigate the composition of the salivary microbiome of 50 healthy Thai children. Methods: A total 76 provinces in Thailand are grouped into 5 geographical clusters based on unique economics, foods and lifestyles. Geographical locations and the results of an oral assessment were also considered. Genomic DNA was extracted from stimulated saliva samples. Subsequently, amplicon libraries were prepared by 16 S Metagenomic Sequencing Library Preparation. The amplicons were sequenced using an Illumina Miseq platform followed by bioinformatics and statistical ***: The correlation between oral hygiene status and caries history varied from r^2=0.887 to r^2=0.999 in the geographical groups, suggesting oral hygiene status a strong association between caries history. Twenty taxonomic groups were found in all subjects and constituted 93.6%-96.5% of the microbiome. Of these, genus Veillonella and Prevotella showed significant differences in their proportions between the geographical groups(P<0.05). Furthermore, the proportion of Veillonella parvula, as well as Rothia aeria and Rothia dentocariosa tended to increase with worse oral hygiene status, which was also related to higher dental caries ***: The differences in the salivary microbiome as related to geographic regions suggest that environmental factors, which may include dietary habits, could influence the predominant bacteria found in the mouth of Thai children, especially the genus Veillonella and Prevotella. The ratio of Veillonella parvula, Rothia aeria and Rothia dentocariosa may be indicators of worse oral hygiene status and future caries in this population.

关键词： Saliva Microbiome Thai Children Region Simplified oral hygiene index

In vitro effects of Salvia officinalis *** oil on Candida albicans

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Asian Pacific Journal of Tropical Biomedicine 2013年第5期3卷 376-380页

作者： Tularat Sookto Theerathavaj Srithavaj Sroisiri thaweboon boonyanit thaweboon Binit Shrestha Maxillofacial Prosthetic Clinic Department of ProsthodonticsFaculty of DentistryMahidol University Department of Oral Microbiology Faculty of DentistryMahidol University

Objective:To determine the anticandidal activities of Salvia officinalis L.(***)essential oil against Candida albicans(***)and the inhibitory effects on the adhesion of *** to polymethyl methacrylate(PMMA)resin ***:Disc diffusion method was first used to test the anticandidal activities of the *** *** oil against the reference strain(ATCC 90028)and 2 clinical strains of *** the minimal inhibitory concentration(MIC)and minimal lethal concentration(MLX)were determined by modified membrane *** adhesion of *** to PMMA resin surface was assessed after immersion with *** *** oil at various concentrations of 1XMIC.0.5XMIC and 0.25XMIC at room temperature for30 ***-way ANOVA was used to compare the Candida cell adhesion with the pretreatment agents and Tukey's test was used for multiple ***:*** *** oil exhibited anticandidal activity against all strains of *** with inhibition zone ranging from 40.5 mm to 19.5 *** MIC and MLC of the oil were determined as 2.780 g/L against all test *** to the effects on *** adhesion to PMMA resin surface,it was found that immersion in the essential oil at concentrations of 1XMIC(2.780 g/L).0.5XMIC(1.390 g/L)and0.25XMIC(0.695 g/L)for 30 min significantly reduced the adhesion of all 3 test strains to PMMA resin surface in a dose dependent manner(P<0.05).Conclusions:*** *** oil exhibited anticandidal activities against *** and had inhibitory effects on the adhesion of the cells to PMMA resin *** further testing and development,*** essential oil may be used as an antifungal denture cleanser to prevent candidal adhesion and thus reduce the risk of candida-associated denture stomatitis.

关键词： Salvia officinalis L. Candida albicans Essential oil PMMA resin Adhesion

Efficacy of xylitol and fluoride mouthrinses on salivary mutans streptococci

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Asian Pacific Journal of Tropical Biomedicine 2011年第6期1卷 488-490页

作者： Malee Arunakul boonyanit thaweboon Sroisiri thaweboon Yuwadee Asvanund Kesinee Charoenchaikorn Department of Pedriatic Dentistry Faculty of DentistryMahidol University Department of Oral Microbiology Faculty of DentistryMahidol University Private Clinic

Objective:To evaluate the level of salivary Mutatis streptococci(MS) after rinsing with xylitol, fluoride,and a combination of xylitol and fluoride solutions,compared with distilled water. Methods:Eighty healthy 8-9 years old subjects with high level of MS(> 10~5 CFU/mL) were equally divided into 4 *** rinsed their moutlis for 1 min with 10 mL of 0.05%(w/v) sodium fluoride(NaF),12.5%(w/v) xylitol or 0.05%(w/v) NoF + 12.5%(w/v) xylitol 3 times daily over 10 *** water rinsed group served as a ***-stimulated whole saliva samples were collected at baseline,5 weeks,and 10 weeks after rinsing to determine the level of salivary MS by culturing on Mitis Salivarius Bacitracin *** statistical significance was calculated by Kruskol Wallis,Mann Whitney U,and Wilcoxon signed-rank tests at a significant level of P< ***:Significant reductions in MS count were observed in subjects using 0.05%NaF + 12.5%xylitol over other groups within 5 weeks and after 10 weeks and 12.5%xylitol alone after 10 weeks compared with ***:The present study provides evidence for the inhibitory effect of xylitol,used in combination with fluoride,delivered in the form of mouthrinse, on salivary MS in the group of schoolchildren.

关键词： Xylitol Fluoride Mouthrinse Mutans streptococci Dental caries Salivary

In vitro antimicrobial effects of grape seed extract on peri-implantitis microflora in craniofacial implants

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Asian Pacific Journal of Tropical Biomedicine 2012年第10期2卷 822-825页

作者： Binit Shrestha M.L.Srithavaj Theerathavaj Sroisiri thaweboon boonyanit thaweboon Maxillofacial Prosthetic Service Faculty of DentistryMahidol University Department of Oral Microbiology Faculty of DentistryMahidol University

Objective:To determine the antimicrobial effects of grape seed on peri-implantitis ***:The grape seed extract was tested against peri-implantitis microflora most commonly found in craniofacial implants including reference strains of Staphylococcus aureus(***),Escherichia coli(***),Candida albicans(***)and clinical strains of ***,Klebsiella pneumonia(***)and Candida parapsilosis(***)by disk diffusion *** inhibitory concentrations(MIC)and minimum cidal concentrations(MCC)were determined using modified agar dilution millpore *** extract was further combined with polyethylene glycol and propylene glycol,and was tested for antimicrobial ***:Grape seed extract showed positive inhibitory effects with *** at MIC of 0.625 mg/mL and MCC of1.25 mg/mL *** the extracts showed minimal or no reactivity against strains of ***,***,*** and *** use of grape seed extract in combination with polyethylene glycol and propylene glycol also showed dose dependent inhibitory effect on ***:The results of the study showed that grape seed has potential antimicrobial effects which can be further studied and developed to be used in the treatment of infected skinabutment interface of craniofacial implants.

关键词： Peri-implantitis Grape seed extract Craniofacial implants Staphylococcus aureus

Antimicrobial effects of silver zeolite,silver zirconium phosphate silicate and silver zirconium phosphate against oral microorganisms

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Asian Pacific Journal of Tropical Biomedicine 2013年第1期3卷 47-52页

作者： Sirikamon Saengmee-anupharb Toemsak Srikhirin boonyanit thaweboon Sroisiri thaweboon Taweechai Amornsakchai Surachai Dechkunakorn Theeralaksna Suddhasthira Faculty of Science Mahidol University Faculty of Dentistry Mahidol University

Objective:To evaluate the antimicrobial activities of silver inorganic materials,including silver zeolite(AgZ),silver zirconium phosphate silicate(AgZrPSi)and silver zirconium phosphate(AgZrp),against oral *** line with this objective,the morphology and structure of each type of silver based powders were also ***:The antimicrobial activities of AgZ,AgZrPSi and AgZrP were tested against Streptococcus mutans,Lactobacillus casei,Candida albicans and Staphylococcus aureus using disk diffusion assay as a screening *** minimum inhibitory concentration(MIC)and minimum lethal concentration(MLC)were determined using the modified membrane *** electron microscope and X-ray diffraction were used to investigate the morphology and structure of these silver ***:All forms of silver inorganic materials could inhibit the growth of all test *** MIC of AgZ,AgZrPSi and AgZrP was 10.0 g/L whereas MLC ranged between 10.0-60.0 g/*** terms of morphology and *** and AgZrP had smaller sized particles(1.5-3.0μm)and more uniformly shaped than ***:Silver inorganic materials in the form of AgZ,AgZrPSi and AgZrP had antimicrobial effects against all test oral microorganisms and those activities may be influenced by the crystal structure of *** results suggest that these silver materials may be useful metals applied to oral hygiene products to provide antimicrobial activity against oral infection.

关键词： Silver zeolite Silver zirconium phosphate silicate Silver zirconium phosphate Oral microorganisms