Effect of lead on IL-8 production and cell proliferation in human oral keratinocytes

作     者：Thaweboon Srosiri Poomsawat Sopee Thaweboon Boonyanit 

作者机构：Department of MicrobiologyFaculty of DentistryMahidol University Yothi StreetBangkok 10400 Thailand Department of Oral PathologyFaculty of DentistryMahidol University Yothi StreetBangkok 10400 Thailand 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2010年第3卷第6期

页      面：475-478页

核心收录：

学科分类：1003[医学-口腔医学] 100302[医学-口腔临床医学] 10[医学] 

主　　题：Cell proliferation Interleukin 8 Lead Oral keratinocytes Ulcerative stomatitis 

摘      要：Objective:To investigate the effect of lead on the production of IL-8 and cell proliferation in normal human oral keratinocytes（NHKs）.Methods:NHKs were prepared as outgrowths from normal human buccal *** cells were treated with three concentrations of lead glutamate(4.5×10-5M,4.5×10-6M and 4.5×10-7M).NHKs grown in glutamic acid were used as *** amounts of IL-8 secreted in the culture supernatants were evaluated at 12 and 24 h using enzyme-linked immunospecific assay（ELISA）.Cell proliferation was determined by the MTT colorimetric *** cultures were used for each experiment,and three independent experiments were *** of variance and Duncan’s multiple range tests were used for statistical ***:An elevation of IL-8 in culture supernatants of NHKs treated with lead at all concentrations at 12 and 24 h after exposure in a dose-dependent manner was revealed.A significant increase in cell numbers was observed only at 24 h exposed to 4.5×10- 5M lead ***:The capacity of NHKs,to secrete IL-8,enhanced by lead glutamate,is demonstrated *** of cell proliferation is revealed only after exposure to high lead *** elevation of secreted IL-8 is a probable initial sign for the acute inflammatory response and may be involved in the pathogenesis of lead stomatitis.