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Down-regulation of miR-622 in gastric cancer promotes cellular invasion and tumor metastasis by targeting ING1 gene

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World Journal of Gastroenterology 2011年第14期17卷 1895-1902页

作者： Xiao-Bo Guo Chang-Qing Jing Le-Ping Li Li Zhang Yu- Long Shi Jin-Shen Wang Jing-Lei Liu chen-Sheng Li Department of Gastrointestinal Surgery Shandong Provincial Hospital Affiliated to Shandong University 324 Jingwu Weiqi Road Jinan 250021 Shandong Province China

AIM:To evaluate the biological and clinical characteristics of miR-622 in gastric cancer. METHODS:We analyzed the expression of miR-622 in 57 pair matched gastric neoplastic and adjacent non-neoplastic tissues by quantitative real-time polymerase chain reaction. Functional analysis of miR-622 expression was assessed in vitro in gastric cancer cell lines with miR-622 precursor and inhibitor. The roles of miR-622 in tumorigenesis and tumor metastasis were analyzed using a stable miR-622 expression plasmid in nude mice. A luciferase reporter assay was used to assess the effect of miR-622 on inhibitor of growth family,member 1 (ING1) expression. RESULTS:Expression of miR-622 was down-regulated in gastric cancer. MiR-622 was found involved in differentia-tion and lymphatic metastasis in human gastric cancer. Ectopic expression of miR-622 promoted invasion,tumorigenesis and metastasis of gastric cancer cells both in vitro and in vivo. ING1 is a direct target of miR-622. CONCLUSION:These findings help clarify the molecular mechanisms involved in gastric cancer metastasis and indicate that miR-622 modulation may be a bona.fide treatment of gastric cancer.

关键词： ING1 肿瘤转移 miR 基因检测胃肿瘤癌组织细胞株胃癌细胞

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瓜蒌皮注射液治疗眩晕的临床效果分析

中国医药科学 2019年第24期9卷 72-74页

作者：曹德峰陈文亚吴波娜史红娟江苏大学附属武进医院徐州医科大学武进临床学院神经内科

目的探讨瓜蒌皮注射液治疗眩晕的临床效果。方法选取2016年1月~2018年12月我院收治的78例眩晕患者,随机分为两组,对照组应用长春西汀治疗,研究组应用瓜蒌皮注射液治疗。评价两组患者疗效,比较两组治疗前后脑供血动脉血液流速变化与患者... 详细信息

目的探讨瓜蒌皮注射液治疗眩晕的临床效果。方法选取2016年1月~2018年12月我院收治的78例眩晕患者,随机分为两组,对照组应用长春西汀治疗,研究组应用瓜蒌皮注射液治疗。评价两组患者疗效,比较两组治疗前后脑供血动脉血液流速变化与患者生活质量。结果研究组治疗效果优于对照组(P<0.05);两组治疗后的血液流变学参数均低于治疗前(P<0.05),且研究组下降更明显(P<0.05);两组治疗后的BI评分明显升高(P<0.05),且研究组升高更为明显(P<0.05)。结论对眩晕患者进行瓜蒌皮注射液治疗,效果理想,可有效改善患者临床症状,改善血液流变学参数,提高生活质量,具有非常高的临床应用价值,值得临床上进一步推广和应用。

关键词：瓜蒌皮注射液眩晕血液流变学生活质量

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权利外观与诈骗罪认定

法学家 2020年第2期 57-72,192,193页

作者：陈少青清华大学法学院

行为人利用虚假的权利外观实施表见行为,第三人对外观产生合理信赖,向行为人给付对价并取得相应财产,根据权利外观理论,民法对第三人财产予以保护,不利后果转由真实权利人承担。但是,刑法的价值取向、司法解释的相关规定以及法律关系强... 详细信息

行为人利用虚假的权利外观实施表见行为,第三人对外观产生合理信赖,向行为人给付对价并取得相应财产,根据权利外观理论,民法对第三人财产予以保护,不利后果转由真实权利人承担。但是,刑法的价值取向、司法解释的相关规定以及法律关系强行转换导致的处罚漏洞,阻断了将该理论适用于犯罪认定的现实可能。在规范层面将财产损害分为损害发生与损害分配,刑民对损害发生的认定相互契合,均将第三人作为表见行为的直接被害人;与此同时,刑法对损害分配不作评价,在法律事实的相对性层面,刑民又各自分立。第三人是遭受财产损失的刑事被害人,在善意取得、表见代理的案件中,行为人通过隐瞒自己并非真实权利人的事实,致使第三人陷入认识错误交付财物,成立诈骗罪。

关键词：权利外观损害发生损害分配直接被害人诈骗罪

Conversion of effector CD4^(+)T cells to a CD8^(+)MHC Ⅱ-recognizing lineage

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Cellular & Molecular Immunology 2021年第1期18卷 150-161页

作者： Elizabeth Robins Ming Zheng Qingshan Ni Siqi Liu chen Liang Baojun Zhang Jian Guo Yuan Zhuang You-Wen He Ping Zhu Ying Wan Qi-Jing Li Department of Immunology Duke University Medical CenterDurhamNC 27710USA Department of Cell Biology National Translational Science Center for Molecular MedicineFourth Military Medical UniversityXi'anChina Biomedical Analysis Center Third Military Medical UniversityChongqingChina Immuno-Oncology Comprehensive Cancer CenterOhio State University Wexner Medical CenterColumbusOH 43210USA

CD4^(+)and CD8^(+)T cells are dichotomous lineages in adaptive *** conventionally viewed as distinct fates that are fixed after thymic development,accumulating evidence indicates that these two populations can exhibit significant lineage plasticity,particularly upon TCR-mediated *** define a novel CD4^(-)CD8αβ^(+)MHC Ⅱ-recognizing population generated by lineage conversion from effector CD4^(+)T ***4-CD8αβ^(+)effector T cells downregulated the expression of T helper cell-associated costimulatory molecules and inaeased the expression of cytotoxic T lymphocyte-associated cytotoxic *** shift in functional potential corresponded with a CD8^(+)-lineage skewed transcriptional ***β repertoire sequencing and in vivo genetic lineage tracing in acutely infected wild-type mice demonstrated that CD4^(-)CD8αβ^(+)effector T cells arise from fundamental lineage reprogramming of bona.fide effector CD4^(+)T *** autophagy via functional deletion of the initiating kinase Vps34 or the downstream enzyme Atg7 enhanced the generation of this cell *** findings suggest that effector CD4^(+)T cells can exhibit a previously unreported degree of skewing towards the CD8^(+)T cell lineage,which may point towards a novel direction for HIV vaccine design.

关键词： CD4^(+)T cell CD8^(+)T cell ThPOK Runx3 autophagy

PTEN and PDCD4 are bona.Fide Targets of microRNA-21 in Human Cholangiocarcinoma

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Chinese Medical Sciences Journal 2012年第2期27卷 65-72页

作者： Chang-zheng Liu Wei Liu Yi Zheng Jin-mei Su Jing--jing Li Lan Yu Xiao-dong He Song-sen chen Department of Biochemistry National Laboratory of Medical Molecular Biology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100005 China Department of General Surgery Department of Rheumatology Peking Union Medical College Hospital Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100730 China

Objective To investigate the expression profile of microRNA-21 in human cholangiocarcinoma tissues and to validate its bona.fide targets in human cholangiocarcinoma cells. Methods The expression profile of microRNA-21 in human cholangiocarcinoma tissues and cholangiocarcinoma cell line, QBC939, was evaluated by using real-time PCR analysis. The bona.fide targets of microRNA-21 were analyzed and confirmed by dual luciferase reporter gene assay and western blot, respectively. The expressional correlation of microRNA-21 and its targets was probed in human cholangiocarcinoma tissues by using real-time PCR, locked nucleic acid in situ hybridization (LNA-ISH), and immunohistochemistry analysis. Results Real-time PCR analysis revealed that microRNA-21 expression depicted a significant up-regulation in human cholangiocarcinoma tissues about 5.6-fold as compared to the matched normal bileduct tissues (P<0.05). The dual luciferase reporter gene assay revealed endogenous microRNA-21 in cholangiocarcinoma cell line, QBC939, inhibited the luciferase reporter activities of wild-type PTEN (P<0.01) and PDCD4 (P<0.05) and had no this effect on mutated PTEN and PDCD4. Moreover, loss of microRNA-21 function led to a significant increase of PTEN and PDCD4 protein levels in QBC939 cells. Elevated microRNA-21 levels were accompanied by marked reductions of PTEN and PDCD4 expression in the same cholangiocarcinoma tissue. Conclusion microRNA-21 expression is up-regulated in human cholangiocarcinoma and PTEN, PDCD4 are direct effectors of microRNA-21.

关键词： PTEN 癌细胞胆管荧光素酶报告基因 microRNA 实时PCR PCR分析蛋白质水平

Down-regulation of Helios Expression in Tregs from Patients with Hypertension

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2018年第1期38卷 58-63页

作者：陈朱越陈凤王燕鸽王定航蒋丽丽程龙献 Laboratory of Cardiovascular Immunology Institute of CardiologyUnion HospitalTongji Medical CollegeHuazhong University of Science and Technology Department of Adult Internal Medicine Hubei Maternal and Child Health Hospital

Regulatory T cells（Tregs） play a pivotal role in the pathological development of ***,a transcription factor from the Ikaros family,was recently reported to be a bona.fide marker for natural Tregs or activated Tregs with suppression function,however,little has been known about its role in *** study was aimed to find whether Helios＋ Tregs really play a vital role in hypertension.A total of 60 hypertension patients,and 46 normotension subjects were enrolled in this *** of different Tregs subsets in peripheral blood were measured by flow *** cytokine level was determined by *** m RNA expression of Foxp3 and Helios in purified CD4＋ T cells was detected by *** of CD4＋Foxp3＋Helios＋ Tregs was decreased significantly in patients with hypertension（62.52%±1.18% vs.71.89%±1.03%,P〈0.01）,and it was correlated with plasma level of IL-10 positively（r=0.505,P〈0.05） and plasma level of IFN-gamma negatively（r=–0.551,P〈0.05）.The m RNA expression of Foxp3（7.23±1.00 vs.10.58±0.54,P〈0.05） and Helios（8.47±0.95 vs.15.52±2.0,P〈0.05） was decreased in CD4＋ T cells from patients with ***＋ Tregs were decreased in patients with hypertension and may play a protective role in hypertension progression.

关键词： hypertension cardiovascular disease regulatory T cells Helios Foxp3

论我国物权变动规则在破产程序中的适用——兼及《物权法司法解释(一)》第六条的解读

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宁夏社会科学 2016年第4期 74-80页

作者：陈政河南工业大学法学院河南郑州450001

我国《物权法》首次系统地规定了动产与不动产的物权变动规则。在债务人进入破产程序后,这些物权变动规则原则上应当得到《企业破产法》的尊重,并作为破产财产认定及破产别除权认定的基本法律依据,但在具备充分而正当理由的前提下,可以... 详细信息

我国《物权法》首次系统地规定了动产与不动产的物权变动规则。在债务人进入破产程序后,这些物权变动规则原则上应当得到《企业破产法》的尊重,并作为破产财产认定及破产别除权认定的基本法律依据,但在具备充分而正当理由的前提下,可以根据实际需要做适当的变通适用。《物权法司法解释(一)》第六条对《物权法》第二十四条进行了解释,澄明特殊动产物权变动也适用"交付生效"规则,圈定了"善意第三人"的范围,对破产实践具有重要的指导意义。

关键词：《物权法》破产程序物权变动《物权法司法解释(一)》

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民法典不当得利返还责任体系之展开

法学研究 2021年第4期43卷 91-110页

作者：陈自强台湾大学法律学院

不当得利虽自民法通则即有规定,但到民法典才对其法律效果有进一步的规定。返还范围区别得利人为善意或恶意,似乎被认为理所当然。不当得利有给付与非给付得利两大类型,已蔚为共识,但如果返还效果规定无视启动返还因素的不同,无差别地... 详细信息

不当得利虽自民法通则即有规定,但到民法典才对其法律效果有进一步的规定。返还范围区别得利人为善意或恶意,似乎被认为理所当然。不当得利有给付与非给付得利两大类型,已蔚为共识,但如果返还效果规定无视启动返还因素的不同,无差别地适用于所有不当得利案型,"非统一说"并无实际意义。双务契约无效或被撤销,已为之给付没有法律依据,构成不当得利,但返还关系之内容,应优先适用民法典第157条,原物返还不能,应折价补偿,而不适用民法典第986条及第987条之规定。非基于双务契约的一方给付案型及侵害权益不当得利案型,为适用该二规定最重要的对象。金钱得利人负有金额返还义务,通常无所受利益不存在的问题,但在溢付薪资或扶养费案例,比较法上常基于不当得利法以外之价值判断,从宽认定所受利益不存在。民法典第987条恶意得利人赔偿损失规定之解释适用,有待厘清,若认为属侵权责任,则不当得利返还责任与侵权责任之关系,剪不断,理还乱。

关键词：不当得利返还责任双务契约无效契约之回复所受利益不存在恶意得利人责任

GCS和NIHSS对基底动脉尖综合征临床转归的预测

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中国神经精神疾病杂志 2010年第3期36卷 141-144页

作者：翟登月魏宁吴波娜卢婷婷刘文华高海宁陈兆耀徐格林刘新峰南方医科大学南京临床学院南京军区南京总医院神经内科南京210002

目的探讨格拉斯哥昏迷评分（Glasgow Coma Scale,GCS）及美国国立卫生院神经功能缺损评分（National Institutes of Health Stroke Scale,NIHSS）对基底动脉尖综合征（top of the basilar syndrome,TOBS）转归的预测价值。方法从南京卒... 详细信息

目的探讨格拉斯哥昏迷评分（Glasgow Coma Scale,GCS）及美国国立卫生院神经功能缺损评分（National Institutes of Health Stroke Scale,NIHSS）对基底动脉尖综合征（top of the basilar syndrome,TOBS）转归的预测价值。方法从南京卒中注册系统中筛选出符合基底动脉尖综合征诊断的64例患者,根据卒中后30d改良Rankin量表评分（modified Rankin Scale,mRS）分为好转组（0-3分）和不良转归组（4-6分）,对两组入院时GCS、NIHSS评分进行回顾性分析。结果不良转归组中GCS分值低于好转组（P〈0.01）;NIHSS分值均高于好转组（P=0.011）。经Logistic回归校正年龄、性别及治疗方式3个因素后,GCS评分OR值0.301（95%CI0.167-0.542）;NIHSS评分OR值1.436（95%CI1.147-1.796）,二者均为独立预测因素。ROC曲线分析显示,以GCS分值10分作为预测TOBS预后的界点,其灵敏度87.9%,特异度83.9%;以NIHSS分值14分作为界点,灵敏度63.6%,特异度77.4%。结论GCS、NIHSS均是基底动脉尖综合征转归重要的预测指标;GCS≤10分、NIHSS≥14分对于基底动脉尖综合征患者不良转归均有重要的预测价值,GCS预测效价高于NIHSS。

关键词：基底动脉尖综合征 GCS NIHSS 预测值