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PIG技术在建筑及市政工程管道施工过程中的应用

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清洗世界 2017年第1期33卷 19-24,33页

作者：张博刘伟王小龙甘肃省第八建设集团有限责任公司甘肃天水741000

文中主要介绍了建筑及市政工程管道的分类、试验规定、通球技术及其在工程管道清洗、检测中的应用,以期为PIG清洗技术在建筑及市政工程管道的应用提供一些参考。

关键词：建筑工程管道市政工程管道通球技术

IL-10基因修饰骨髓源性肝干细胞移植对肝纤维化大鼠细胞外基质积聚的影响

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世界华人消化杂志 2009年第22期17卷 2231-2236页

作者：兰玲陈源文孙超刘博伟孙巧玲河南省人民医院二内消化科河南省郑州市450003 上海交通大学医学院附属新华医院消化内科上海市200092 上海交通大学医学院附属第六人民医院老年科上海市200233

目的:评价IL-10基因修饰骨髓源性肝干细胞(BDLSCs)移植对肝纤维化大鼠细胞外基质(ECM)积聚的影响.方法:利用免疫磁珠细胞分选(magnetic bead cell sorting,MACS)方法分选大鼠β2m-/Thy-1+BDLSCs.将腺病毒介导的IL-10基因转导至BDLSCs,采... 详细信息

目的:评价IL-10基因修饰骨髓源性肝干细胞(BDLSCs)移植对肝纤维化大鼠细胞外基质(ECM)积聚的影响.方法:利用免疫磁珠细胞分选(magnetic bead cell sorting,MACS)方法分选大鼠β2m-/Thy-1+BDLSCs.将腺病毒介导的IL-10基因转导至BDLSCs,采用ELISA法检测IL-10蛋白分泌水平.将大鼠随机分为4组:正常组、模型组、BDLSCs组及BDLSCs/IL-10组.采用PCR法检测Y染色体性别决定基因Sry;采用VG染色法观测肝组织胶原沉积面积;Western blot法检测肝组织α-平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)表达;采用碱水解法检测肝组织羟脯氨酸(hydroxyproline,Hyp)浓度;ELISA法检测血清透明质酸(HA)、层粘连蛋白(LN)和Ⅲ型前胶原(PCⅢ)等细胞外基质含量.结果:MASC法能成功分选纯化***-10修饰BDLSCs持续高浓度分泌IL-10蛋白至胞外,移植后能顺利种植于肝内,减轻胶原沉积,减少α-SMA表达从而抑制肝星状细胞活化,与BDLSCs组比较,BDLSCs/IL-10组大鼠肝组织Hyp水平显著降低(255.0±50.5μg/gvs373.0±26.7μg/g,P<0.01),血清HA,LN及PCⅢ水平也有所降低,差异具有统计学意义(40.5±7.7μg/Lvs79.4±10.3μg/L,61.5±16.4μg/Lvs77.7±12.6μg/L,14.3±0.8μg/Lvs14.9±1.5μg/L,P<0.01或0.05).结论:IL-10基因修饰BDLSCs移植能有效减少肝纤维化大鼠ECM的积聚,为治疗肝纤维化提供了新思路.

关键词：骨髓源性肝干细胞白介素10 肝纤维化细胞外基质

Gut microbiota-astrocyte axis: new insights into age-related cognitive decline

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Neural Regeneration Research 2025年第4期20卷 990-1008页

作者： Lan Zhang Jingge wei Xilei liu Dai Li Xiaoqi Pang Fanglian Chen Hailong Cao Ping Lei Haihe Laboratory of Cell Ecosystem Department of GeriatricsTianjin Medical University General HospitalTianjinChina Tianjin Geriatrics Institute Tianjin Medical University General HospitalTianjinChina Department of Gastroenterology and Hepatology Tianjin Medical University General HospitalNational Key Clinical SpecialtyTianjin Institute of Digestive DiseasesTianjin Key Laboratory of Digestive DiseasesTianjinChina Department of Urology Tianjin Medical University General HospitalTianjinChina Tianjin Neurological Institution Tianjin Medical University General HospitalTianjinChina

With the rapidly aging human population,age-related cognitive decline and dementia are becoming increasingly prevalent *** is considered the main risk factor for cognitive decline and acts through alterations in the composition of the gut microbiota,microbial metabolites,and the functions of *** microbiota–gut–brain axis has been the focus of multiple studies and is closely associated with cognitive *** article provides a comprehensive review of the specific changes that occur in the composition of the gut microbiota and microbial metabolites in older individuals and discusses how the aging of astrocytes and reactive astrocytosis are closely related to age-related cognitive decline and neurodegenerative *** article also summarizes the gut microbiota components that affect astrocyte function,mainly through the vagus nerve,immune responses,circadian rhythms,and microbial ***,this article summarizes the mechanism by which the gut microbiota–astrocyte axis plays a role in Alzheimer’s and Parkinson’s *** findings have revealed the critical role of the microbiota–astrocyte axis in age-related cognitive decline,aiding in a deeper understanding of potential gut microbiome-based adjuvant therapy strategies for this condition.

关键词： age aging Alzheimer’s disease astrocytes cognitive decline dementia gut microbiota gut–brain axis microbial metabolites neuroinflammation Parkinson’s disease

Enhancement of motor functional recovery in thoracic spinal cord injury: voluntary wheel running versus forced treadmill exercise

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Neural Regeneration Research 2025年第3期20卷 836-844页

作者： Do-Hun Lee Dan Cao Younghye Moon Chen Chen Nai-Kui liu Xiao-Ming Xu wei Wu Spinal Cord and Brain Injury Research Group Stark Neurosciences Research InstituteIndiana University School of MedicineIndianapolisINUSA Department of Neurological Surgery Indiana University School of MedicineIndianapolisINUSA Department of Neuroscience Baylor College of MedicineHoustonTXUSA

Spinal cord injury necessitates effective rehabilitation strategies, with exercise therapies showing promise in promoting recovery. This study investigated the impact of rehabilitation exercise on functional recovery and morphological changes following thoracic contusive spinal cord injury. After a 7-day recovery period after spinal cord injury, mice were assigned to either a trained group(10 weeks of voluntary running wheel or forced treadmill exercise) or an untrained group. Bi-weekly assessments revealed that the exercise-trained group, particularly the voluntary wheel exercise subgroup, displayed significantly improved locomotor recovery, more plasticity of dopaminergic and serotonin modulation compared with the untrained group. Additionally, exercise interventions led to gait pattern restoration and enhanced transcranial magnetic motor-evoked potentials. Despite consistent injury areas across groups, exercise training promoted terminal innervation of descending axons. In summary, voluntary wheel exercise shows promise for enhancing outcomes after thoracic contusive spinal cord injury, emphasizing the role of exercise modality in promoting recovery and morphological changes in spinal cord injuries. Our findings will influence future strategies for rehabilitation exercises, restoring functional movement after spinal cord injury.

关键词： behavioral assessment motor function neural plasticity running wheel exercise spinal cord injury treadmill exercise voluntary exercise

心肌I_(K1)激动剂zacopride抗左甲状腺素致大鼠心室重构作用的研究

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中国药理学通报 2017年第5期33卷 641-646页

作者：郭云飞杨迎刘福翟旭雯张研李盼张莉吴博威刘清华山西医科大学病理生理学教研室山西太原030001 山西医科大学生理学教研室山西太原030001 山西省儿童医院临床检验中心山西太原030001

目的探讨心肌内向整流钾通道(I_(K1))激动剂zacopride对左甲状腺素致大鼠心室重构的抑制作用及可能的机制。方法 SD大鼠,随机分为对照组,左甲状腺素模型组,zacopride 5、15、50μg·kg^(-1)干预组,zacopride(15μg·kg^(-1))+氯喹(7.5μ... 详细信息

目的探讨心肌内向整流钾通道(I_(K1))激动剂zacopride对左甲状腺素致大鼠心室重构的抑制作用及可能的机制。方法 SD大鼠,随机分为对照组,左甲状腺素模型组,zacopride 5、15、50μg·kg^(-1)干预组,zacopride(15μg·kg^(-1))+氯喹(7.5μg·kg^(-1))组和卡托普利阳性对照组。连续给药10 d。超声测量大鼠左室舒张期内径(LVIDd)、左室收缩期内径(LVIDs)、室间隔厚度(IVS)、左室射血分数(EF)和左室短轴缩短率(FS);测心肌肥厚指数;Langendorff急性分离成年大鼠单个心室肌细胞,全细胞膜片钳技术测量各组I_(K1)电流、L-型钙通道(I_(Ca-L))电流的变化;Fluo-4激光共聚焦显微镜观察细胞形态,测胞内游离钙离子浓度([Ca^(2+)]_i)。结果与对照组比,左甲状腺素模型组全心和左心肥厚指数明显增加,LVIDd、LVIDs增大,IVS增厚,EF和FS明显降低(P<0.01);I_(K1)电流减小,ICa-L增加(P<0.01);心室肌细胞增宽、肥大,[Ca^(2+)]_i增高(P<0.01)。Zacopride干预后,各指标均明显改善,15μg·kg^(-1)为最适效应剂量。氯喹7.5μg·kg^(-1)作为I_(K1)通道相对特异性阻断剂,可阻断zacopride对I_(K1)通道的激动效应,明显逆转zacopride的减轻钙超载和抗心室重构作用。结论 Zacopride可能通过选择性激动心肌I_(K1),减轻细胞内钙超载而逆转左甲状腺素诱发的心室重构。

关键词：内向整流钾通道 zacopride 左甲状腺素心室重构心肌肥大钙超载

Small modifications of endo-functionalized cavities lead to large changes in molecular interaction in water

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Science China Chemistry 2025年第1期 369-376页

作者： Song-Meng Wang Hao Nian Yan-Fang Wang Li-Shuo Zheng Yu-Tao Zheng Yi-wei Dong Liping Huang Xiaoping Wang wei Jiang liu-Pan Yang Department of Chemistry Southern University of Science and Technology School of Chemistry and Pharmaceutical Sciences State Key Laboratory for Chemistry and Molecular Engineering of Medicinal ResourcesGuangxi Normal University School of Pharmaceutical Science Hengyang Medical School University of South China

Studying molecular recognition in aqueous environments is crucial for understanding biological processes. Herein, we present a series of N-doped endo-functionalized anthracene-based cavities by introducing inward-directed pyridine units. The subtle modifications can significantly affect the binding properties of these receptors, which is similar to the mutations in biological systems. Combining theoretical calculations and molecular recognition experiments demonstrated that these “mutations” have an impact on the hydrophobicity and charge distribution of the hosts, consequently significantly influencing molecular *** is crucial for understanding the intricate mechanisms of molecular-level interactions in biological receptors.

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Decreased levels of phosphorylated synuclein in plasma are correlated with poststroke cognitive impairment

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Neural Regeneration Research 2025年第9期20卷 2598-2610页

作者： Yi Wang Yuning Li Yakun Gu wei Ma Yuying Guan Mengyuan Guo Qianqian Shao Xunming Ji Jia liu Department of Clinical Laboratory Beijing Bo’ai HospitalChina Rehabilitation Research CenterCapital Medical UniversityBeijingChina Beijing Institute of Brain Disorders Laboratory of Brain DisordersMinistry of Science and TechnologyCollaborative Innovation Center for Brain DisordersBeijing Advanced Innovation Center for Big Data-based Precision MedicineCapital Medical UniversityBeijingChina Department of Neurosurgery Xuanwu HospitalCapital Medical UniversityBeijingChina

Poststro ke cognitive impairment is a major secondary effect of ischemic stroke in many patients;however,few options are available for the early diagnosis and treatment of this *** aims of this study were to(1)determine the specific relationship between hypoxic andα-synuclein during the occur of poststroke cognitive impairment and(2)assess whether the serum phosphorylatedα-synuclein level can be used as a biomarker for poststro ke cognitive *** found that the phosphorylatedα-synuclein level was significantly increased and showed pathological aggregation around the cerebral infa rct area in a mouse model of ischemic *** addition,neuronalα-synuclein phosphorylation and aggregation were observed in the brain tissue of mice subjected to chronic hypoxia,suggesting that hypoxia is the underlying cause ofα-synuclein-mediated pathology in the brains of mice with ischemic *** phosphorylatedα-synuclein levels in patients with ischemic stroke were significantly lower than those in healt hy subjects,and were positively correlated with cognition levels in patients with ischemic ***,a decrease in serum high-density lipoprotein levels in stroke patie nts was significantly correlated with a decrease in phosphorylatedα-synuclein *** ischemic stroke mice did not show significant cognitive impairment or disrupted lipid metabolism 14 days after injury,some of them exhibited decreased cognitive function and reduced phosphorylatedα-synuclein *** together,our results suggest that serum phosphorylatedα-synuclein is a potential biomarker for poststroke cognitive impairment.

关键词： biomarker high-density lipoprotein ischemic stroke phosphorylatedα-synuclein poststroke cognitive impairment

胸腔镜胸膜固定术治疗大量恶性胸腔积液的临床疗效及安全性分析

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中国实用医药 2017年第6期12卷 25-26页

作者：曲博姜威刘永欣沈阳医学院附属中心医院 110024

目的分析胸腔镜胸膜固定术治疗大量恶性胸腔积液(MPE)的临床疗效及安全性。方法 98例大量恶性胸腔积液患者作为研究对象,使用随机数表法分为对照组与观察组,各49例。给予对照组胸腔闭式引流术,给予观察组胸腔镜胸膜固定术,进行为期12周... 详细信息

目的分析胸腔镜胸膜固定术治疗大量恶性胸腔积液(MPE)的临床疗效及安全性。方法 98例大量恶性胸腔积液患者作为研究对象,使用随机数表法分为对照组与观察组,各49例。给予对照组胸腔闭式引流术,给予观察组胸腔镜胸膜固定术,进行为期12周的随访,对比两组治疗效果及并发症发生情况。结果观察组总有效率为95.92%,明显高于对照组的73.47%,差异具有统计学意义(P<0.05);观察组并发症发生率为6.12%,低于对照组22.45%,差异具有统计学意义(P<0.05)。结论大量恶性胸腔积液患者采用胸腔镜胸膜固定术治疗的临床疗效显著优于胸腔闭式引流术,兼具较高安全性,值得推广。

关键词：恶性胸腔积液胸腔镜胸膜固定术闭式引流术疗效

Double [5]carbohelicene: facile synthesis, chiroptical properties,isomerization study, and lasing application

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Science China Chemistry 2025年第1期 233-240页

作者： Peipei liu Yantong Li Meng-Xiang Wu Hao Kang Xiao-Li Zhao Lin Xu Linlin liu Xiaodong Li Junfeng Fang Zhiwei Fang Ya Cheng Hai-bo Yang Huakang Yu Xueliang Shi State Key Laboratory of Petroleum Molecular & Process Engineering Shanghai Key Laboratory of Green Chemistry and Chemical ProcessesSchool of Chemistry and Molecular Engineering East China Normal University School of Physics and Optoelectronics South China University of Technology Institute of Polymer Optoelectronic Materials & Devices State Key Laboratory of Luminescent Materials & Devices South China University of Technology School of Physics and Electronic Science Engineering Research Center of Nanophotonics & Advanced Instrument Ministry of EducationEast China Normal University State Key Laboratory of Precision Spectroscopy School of Physics and Electronic Science East China Normal University

Double carbohelicenes have attracted considerable attention due to their aesthetic structures, distinct π-conjugation extension, inherent chirality, and intriguing optical and electronic properties. Herein, the concise de novo synthesis of a new double[5]carbohelicene 1 together with its chiroptical properties, isomerization process and lasing application is presented. 1 was synthesized by a simple protocol involving the formation of a 6,6′-bipentacene as the key intermediate. It is worth noting that1 existed as two diastereomers 1-PP/MM and 1-PM, which were successfully isolated and unambiguously confirmed by Xray crystallography. The optical resolution of the racemic 1-PP/MM was successfully achieved by chiral-phase highperformance liquid chromatography（HPLC）, and 1-PP and 1-MM were characterized by circular dichroism. Interestingly, it was found that 1-PP/MM could be completely isomerized to 1-PM upon heating, and the detailed thermodynamics and kinetics of the isomerization process were systematically investigated. 1-PM exhibited deep-red luminescence with emission maximum at 723 nm and fluorescence quantum yield as high as 32.95%. Consequently, we can showcase the lasing application of 1-PM by doping with epoxy resin to assemble a bottle microlaser spontaneously, which the lasing wavelength is 796.6 nm at a threshold of 0.8 m J cm-2. We believe that our studies, including the facile synthesis methodology, detailed isomerization studies, and lasing application, will shed some light on the design and synthesis of novel carbohelical systems with more functions.

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