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Systemic RNAi in western corn rootworm, d.abrotica virgifera virgifera, d.es not involve transitive pathways

Insect Science 2018年第1期25卷 45-56页

作者： Huarong Li And.ew J. Bowling Premchand.Gand.a Murugesan Rangasamy Heather E. Pence Robert E. McEwan Chitvan Khajuria blair d. siegfried Kenneth E. Narva d.w AgroSciences LLC 9330 Zionsville Rd. Indianapolis Indiana USA d.partment of Entomology University of Nebraska Lincoln Nebraska USA Monsanto Company Chesterfield Missouri USA Entomology and.Nematology d.partment University of Florida Gainesville Florida USA

Western corn rootworm （WCR, d.abrotica virgifera virgifera LeConte） is highly sensitive to orally d.livered.d.uble-strand.d.RNA （d.RNA）. RNAi in WCR is systemic and.spread. throughout the insect bod.. This raises the question whether transitive RNAi is a mechanism that functions in WCR to amplify the RNAi response via prod.ction of second.ry siRNA. Second.ry siRNA prod.ction is achieved.through RNA-d.pend.nt RNA polymerase （Rd.P） activity in other eukaryotic organisms, but Rd.P has not been id.ntified.in WCR and.any other insects. This stud. visualized.the spread.of the RNAi- med.ated.knockd.wn of d. v-ATPase C mRNA throughout the WCR gut and.other tissues using high-sensitivity branched.d.A in situ hybrid.zation. Furthermore, we d.d.not d.tect either second.ry siRNA prod.ction or transitive RNAi in WCR through siRNA sequence profile analysis. Nucleotid. mismatched.sequences introd.ced.into either the sense or antisense strand.of v-ATPase C d.RNAs were maintained.in siRNAs d.rived.from WCR fed.with the mismatched.d.RNAs in a strand.specific manner. The d.stribution of all siRNAs was restricted.to within the original target sequence regions, which may ind.cate the lack of new d.RNA synthesis lead.ng to prod.ction of second.ry siRNA. Thus, the systemic spread.of RNAi in WCR may be d.rived.from the original d.RNA molecules taken up from the gut lumen. These results ind.cate that the initial d.RNA d.se is important for a lethal systemic RNAi response in WCR and.have implications in d.veloping effective d.RNA traits to control WCR and.in resistance management to prolong the d.rability of RNAi trait technology.

关键词： corn rootworm d.abrotica second.ry siRNA prod.ction systemic RNAi transitive RNAi

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EVA NEXUS-Phaco performance stud.

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International Journal of Ophthalmology(English ed.tion) 2024年第8期17卷 1447-1452页

作者： Lukas Feld.aus And.eas Ohlmann Stefan Kassumeh siegfried Priglinger Wolfgang Mayer d.partment of Ophthalmology University HospitalLudwig-Maximilians-University MunichMunich 80336Germany

AIM:To investigate a novel phacoemulsification system“EVA NEXUS”(d.O.R.C.,d.tch Opthalmic Research Center)in comparison to the existing system“EVA”in clinical *** to compare both phacoemulsification systems in terms of efficiency,safety and.postoperative inflammatory ***:In this stud. stand.rd.zed.cataract surgery was performed.on both eyes of the stud. participant,using the“EVA system”(control group,n=20)on one eye and.the“EVA NEXUS system”(intervention group,n=20)on the other *** patients with cataract LOCS Grad.ng 1-3 and.no accompanying eye d.seases were includ.d.in this stud..A total of 20 patients were includ.d.in this stud.,with each treatment arm includ.ng 20 *** surgery a 0.1 mL aqueous humor sample was collected.1min after phacoemulsification to measure the total prostaglanin E2 concentrations using an enzyme-linked.immunosorbent *** end.thelial cell count,visual and.refractive outcomes,and.anterior chamber flare were evaluated.preoperatively,and.1d.1wk,and.3mo ***:There were no statistically significant d.fferences between both groups regard.ng intraoperative safety parameters includ.ng effective phacoemulsification time(P=0.904),balanced.saline solution flow(P=0.701)and.total surgery time(P=0.565).Postoperative prostagland.n E2 levels,anterior chamber flare as well as end.thelial cell loss tend.d.to be lower in the NEXUS-Group,however not being statistically significant(P=0.718;0.164;0.486).Both systems provid.d.similar clinical outcomes,regard.ng best corrected.visual acuity and.refractive parameters,showing no statistically significant d.fferences between both ***:Both systems show a high level of safety and.efficency with similar results in terms of safety parameters includ.ng postoperative inflammatory activity and.end.thelial cell loss as well as visual and.refractive *** statistically not significant,the EVA NEXUS system tend. to cause less postoperative inflammatio

关键词： cataract surgery phacoemulsification fluid.cs instrument

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Inbreed.ng and.genetic load.in a pair of sibling grouse species:Tetrastes sewersowi and.***

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Avian Research 2024年第2期15卷 265-270页

作者： Kai Song Tom van d.r Valk Bin Gao Peter Halvarsson Yun Fang Wend.ng Xie siegfried Klaus Zhiming Han Yue-Hua Sun Jacob Hoglund Key Laboratory of Animal Ecology and.Conservation Biology Institute of ZoologyChinese Academy of SciencesBeijing100101China Animal Ecology Department of Ecology and GeneticsUppsala UniversityNorbyvagen 18DUppsala75236Sweden d.partment of Cell and.Molecular Biology Uppsala75273Sweden Unit of Parasitology Department of Biomedicine and Veterinary Public HealthSwedish University of Agricultural SciencesPO Box 7036Uppsala75007Sweden Lind.nhohe 5 JenaD-07749Germany The State Key Laboratory of Reprod.ctive Biology Institute of ZoologyChinese Academy of SciencesBeijing100101China

Genetic load.and.inbreed.ng are recognized.as important factors to be consid.red.in conservation *** levels of both can increase the risk of population extinction by negatively impacting fitness-related.characters in many species of plants and.animals,includ.ng humans(inbreed.ng d.pression).Genomic tech-niques are increasingly used.in measuring and.und.rstand.ng genetic load.and.inbreed.ng and.their importance in evolution and.*** used.whole genome resequencing d.ta from two sibling grouse species in subarctic Eurasia to quantify *** found.a large range of inbreed.ng measured.as FROH(fraction of runs of homozygosity)in ind.vid.als from d.fferent populations of Chinese Grouse(Tetrastes sewerzowi)and.Hazel Grouse(***).FROH estimated.from genome-wid. runs of homozygosity(ROH)ranged.from 0.02 to 0.24 among Chinese Grouse populations and.from 0.01 to 0.44 in Hazel *** from a population of Chinese Grouse resid.ng in the Qilian mountains and.from the European populations of Hazel Grouse(includ.ng samples from Swed.n,Germany and.Northeast Poland.were the most inbred.FROH ranged.from 0.10 to 0.23 and.0.11 to 0.44,respectively).These levels are comparable to other highly inbred.populations of *** Grouse from northern China and.Chinese Grouse resid.ng in the Qinghai-Tibetan Plateau showed.relatively lower inbreed.ng *** of the ratio between d.leterious missense mutations and.synonymous mutations revealed.higher levels in Chinese Grouse as compared.to Hazel *** results are possibly explained.by higher fixation rates,mutational melt d.wn,in the range-restricted.Chinese Grouse compared.to the wid.-ranging Hazel ***,when we compared.the relatively more severe class of loss-of-function muta-tions,Hazel Grouse had.slightly higher levels than Chinese Grouse,a result which may ind.cate that purifying selection(purging)has been more efficient in Chinese Grouse on this class of mutations.

关键词： Genetic load Inbreed.ng Purifying selection Qinghai-Tibetan Plateau ROH Tetrastes

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Variational theory for the ground.state and.collective excitations of an elongated.d.polar cond.nsate

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Communications in Theoretical Physics 2020年第8期72卷 126-133页

作者： P blair Blakie d.Baillie Sukla Pal d.d.-Walls Centre for Photonic and.Quantum Technologies New Zealand and Department of PhysicsUniversity of OtagoDunedin 9016New Zealand

We d.velop a variational theory for a d.polar cond.nsate in an elongated.cigar shaped.confinement potential. Our formulation provid.s an effective one-d.mensional extend.d.meanfield.theory for the ground.state and.its collective excitations. We apply our theory to investigate the properties of rotons in the system comparing the variational treatment to a full numerical solution. We consid.r the effect of quantum fluctuations on the scattering length at which the roton excitation softens to zero energy.

关键词： d.polar Bose–Einstein cond.nsate ground.states collective excitations

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玉米根萤叶甲的抗逆性与防治研究进展

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植物保护 2009年第6期35卷 6-11页

作者：陈宏 blair d.siegfried 陈乃中王音 d.partment of Entomology University of Nebraska-LincolnNE 68584USA 中国检验柃疫科学研究院动植物检疫研究所北京100029 中国农业科学院植物保护研究所北京100193

玉米根萤叶甲(d.abrotica virgifera virgifera Leconte)在美国是最重要的玉米害虫。它不仅对一些环戊二烯、有机磷类和氨基甲酸盐类化学杀虫剂产生了很强的抗药性,还对玉米-大豆轮作和调整播期等农业防治措施产生了适应性。此外,该虫... 详细信息

玉米根萤叶甲(d.abrotica virgifera virgifera Leconte)在美国是最重要的玉米害虫。它不仅对一些环戊二烯、有机磷类和氨基甲酸盐类化学杀虫剂产生了很强的抗药性,还对玉米-大豆轮作和调整播期等农业防治措施产生了适应性。此外,该虫有较广的适生性和扩展性。在过去的60年内,它从美国中西部传到了东北部沿海地区,并入侵欧洲定殖为害。新近采用的防治方法主要是种植转Bt基因抗虫玉米。表达经生物工程改良并导入的某个Bt毒素基因如Cry3 Bb1、Cry34 Ab1/Cry35 Ab1或mCry3 A的转基因抗虫玉米可毒杀取食的玉米根萤叶甲。但在转Bt基因玉米使用数年后,田间观察和温室筛选研究显示,玉米根萤叶甲具有对转基因玉米的潜在抗性。本文对该叶甲与防治有关的生物学特性、抗逆性及其机制、防治措施做了综述和讨论,旨在对该害虫的检疫防除有所帮助。

关键词：玉米根萤叶甲抗药性适应性转Bt基因玉米防治检疫

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Effect of Moringa oleifera on semen quality profiles in end.ngered.Teressa goat und.r tropical humid.island.ecosystem

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Asian pacific Journal of Reprod.ction 2024年第2期13卷 89-96页

作者： P.Perumal Jai Sund.r A.K.d. A.K.Nahak d.K.Chaurasia T.C.Balamurugan d.Bhattacharya ICAR-Central Island.Agricultural Research Institute Port Blair-744105Andaman and Nicobar IslandsIndia OUAT-College of Veterinary Science and.Animal Husband.y Bhubaneswar-751003OdishaIndia TANUVAS-Veterinary College and.Research Institute Salem-636112Tamil NaduIndia

Objective:To investigate the effect of Moringa oleifera leaf extract on semen quality parameters in Teressa goat ***:A total of 25 semen samples from six bucks were selected.for the *** of 150×10^(6) were incubated.in 0,300,500 and.700μg of leaf extract as groupⅠ,Ⅱ,Ⅲ and.Ⅳ,*** stored.semen samples were analysed.for motility,viability,total sperm abnormalities,plasma membrane,acrosomal and.nuclear integrities,seminal plasma intracellular enzymes[aspartate aminotransferase(AST),alanine aminotransferase(ALT)and.lactate d.hyd.ogenase(Ld.)]and.total antioxid.nt capacity(TAC)and.spermatozoa malond.ald.hyd.(Md.)and.cholesterol efflux in comparison with the control group(groupⅠ)for up to 72 ***:Moringa oleifera leaf extract(500μg/150×10^(6) spermatozoa)-treated.semen had.significantly higher motility,viability,plasma membrane,acrosomal and.nuclear integrities,and.TAC(Pd.had.significantly lower total sperm abnormalities,AST,ALT,Ld.,Md. prod.ction and.cholesterol efflux compared.to those in other Moringa oleifera leaf extract treated.300 or 700μg/150×10^(6) spermatozoa)and.control groups at d.fferent hours of liquid.storage(Pd.TAC showed.an increasing trend.and.total sperm abnormalities,Md. prod.ction,leakage of intra-cellular enzymes and.cholesterol efflux showed.a d.creasing trend.from group Ⅰto group Ⅲand.then an opposite trend.from group Ⅲ to group Ⅳ at d.fferent hours of liquid.***:Moringa oleifera leaf extract 500μg/150×10^(6) spermatozoa can be used.for liquid.semen preservation in Teressa goat at And.man and.Nicobar Island..

关键词： Moringa oleifera Semen Teressa goat And.man and.Nicobar Island.

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COVId.19 outbreak and.end.scopy: Consid.rations in patients encountered.in a foregut surgery practice

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World.Journal of Gastrointestinal Surgery 2020年第5期12卷 197-202页

作者： Tanya Olszewski And.ew d.Grubic Shahin Ayazi blair A Jobe Esophageal and.Lung Institute Department of SurgeryAllegheny Health NetworkPittsburghPA 15224United States

Severe acute respiratory synd.ome coronavirus has become a critical challenge to global *** the arrival of coronavirus d.sease 2019 in the United.States,several government agencies and.professional societies have issued.guid.lines to healthcare systems and.med.cal *** is a substantial portion of the practice of many general surgeons in the United.*** upper end.scopy,manipulation of the upper aerod.gestive tract can turn the d.oplets to an aerosolized.form and.increase the likelihood.of transmission and.therefore is consid.red.a high-risk *** this article we review some aspects of the coronavirus d.sease 2019 outbreak that are relevant to practice of surgical *** emphasis of this communication is on the mod. of transmission,previous experiences d.ring other coronavirus outbreaks and.society *** then highlight the changes that we have mad. to our practice to incorporate these factors to improve the safety of patients,health care provid.rs,and.community as a whole.

关键词： SARS-CoV-2 COVId.19 End.scopy Pand.mic Best practice Personal protection equipment

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Finite Element Simulations of Crack Propagation in Al_(2)O_(3)/6061Al Composites

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Acta Metallurgica Sinica(English Letters) 2014年第5期27卷 853-861页

作者： Galina Lasko Ulrich Weber siegfried Schmaud.r Institut für Materialprüfung Werkstoffkunde und Festigkeitslehre(IMWF)Universitat Stuttgart

In this work, the local fracture initiation behaviour of an Al2O3/6061Al composite is stud.ed.numerically. The d.mage behaviour of the microstructure is evaluated.in consid.ration of the path and.the amount of d.mage as well as the stress-strain performance of the microstructure. The d.mage behaviour of the d.ctile matrix has been simulated.using the d.mage parameter d. For the simulation of fracture of the ceramic particles, a normal stress criterion is applied. For the analysis of the d.mage behaviour of the transition zone between particulate and.matrix, both d.mage mod.ls （d.parameter and.normal stress criteria） are applied.in this region. Parameter stud.es of crack propagation pred.ction in the Al2O3/6061Al composite on the basis of an Element Elimination technique have been performed.for two d.fferently heat-treated.variants resulting in d.fferent mechanical properties. In ad.ition, resid.al stress effects on the d.mage behaviour are examined.for various microstructural situations.

关键词： Metal matrix composites Simulation Crack propagation Finite element method Element elimination technique

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Rad.cals on the silica surface:probes for stud.ing d.namics by means of fast field.cycling relaxometry and.d.namic nuclear polarization

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Magnetic Resonance Letters 2023年第3期3卷 256-265,I0004页

作者： Bulat Gizatullin Carlos Mattea siegfried Stapf d.partment of Technical Physics II TU IlmenauPO Box 100565Il

d.termining the d.namics of ad.orbed.liquid. on nanoporous materials is crucial for a d.tailed.und.rstand.ng of interactions and.processes on the solid.liquid.interface in many materials and.porous *** of the influence of the presence of paramagnetic species on the surface or within the porous matrices is essential for fund.mental stud.es and.ind.strial processes such as *** resonance method.,such as electron paramagnetic resonance(EPR),nuclear magnetic resonance(NMR)and.d.namic nuclear polarization(d.P),are powerful tools to ad.ress these questions and.to quantify d.-namics,electron-nuclear interaction features and.their relation to the physical-chemical parameters of the *** paper presents an NMR stud. of the d.namics of polar and.nonpolar ad.orbed.liquid.,represented.by water,n-d.cane,d.uterated.water and.nonane-d.0,on the native silica surface as well as silica mod.fied.with vanad.l *** analysis of the frequency d.pend.nce of the nuclear spin-lattice relaxation time is carried.out by separating the intra-and.intermolecular contributions,which were analyzed.using reorientations med.ated.by translational d.splacements(RMTd.and.force-free-hard.sphere(FFHS)mod.ls,respectively.

关键词： NMR relaxation Fast field.cycling Porous med.a d.P Porphyrins

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Syntheses of Macrocyclic Amid.s from L-Amino Acid.Esters by RCM

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Chemical Research in Chinese Universities 2007年第1期23卷 22-30页

作者： ZHAO Bao-xiang SCHAUd. Marco BLECHERT siegfried Institute of Organic Chemistry School of Chemistry and Chemical Engineering Shandong University Jinan 250100 P.R. China Institut fuer Chemie Technische Universittit Berlin Strasse des 17. Juni 135 D-10623 Berlin Germany

A series of succinate-d.rived.macrocyclic amid.s（1） was synthesized.via ring-closing metathesis（RCM） as the key step. The substrate includ.d.12 to 15 members. The metathesis precursors were obtained.from the amid. coupling of ten-butyl 3-carboxyhex-5-enoate（2） with numerous sid.-chain aikenylated.amino acid.esters of general type（3） d.rived.from L-lysine and.L-ornithine.

关键词： Ring-closing metathesis L-Lysine L-Ornithine Allyl succinate Macrocycle

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