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Reoperation for early postoperative complications after gastric cancer surgery in a Chinese hospital

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World Journal of Gastroenterology 2010年第1期16卷 98-103页

作者： birendra kumar sah Department of General Surgery Ruijin HospitalShanghai Jiaotong UniversitySchool of MedicineShanghai Institute of Digestive Surgery

AIM:To investigate the occurrence of postoperative complications of gastric cancer surgery,and analyze the potential causes of reoperation for early postoperative complications. METHODS:A total of 1639 patients who underwent radical or palliative gastrectomies for gastric cancer were included in the *** study endpoint was the analysis of postoperative complications in inpatients. RESULTS:About 31%of patients had early postoperative complications,and complications of infection occurred most ***-abdominal hemorrhage and anastomotic leak were the main causes of reoperation,which accounted for about 2.2%.Mortality was 11.1%in the reoperation group,but was only 0.8%in other *** duration of postoperative stay in hospital was significantly longer and the total expenditure was markedly higher in the patients who underwent reoperation(P<0.001).There was no significant association of any available factors in this study with the high rate of ***:Reoperation significantly increases the mortality rate and raises the burden of the surgical *** prospective studies are required to explore the potential risk factors.

关键词： Reoperation Gastric cancer Surgery Postoperative complications

A reliable auditing of postop complication in gastric cancer surgery

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国际外科学杂志 2007年第11期34卷 788-792,F0003-F0004页

作者： birendra kumar sah WANG Xiao-Yan ZHU Zheng-Gang YAN-Min CHEN Jun XIANG MingDepartment of General Surgery Rui Jin Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai Institute of Digestive Surgery Shanghai 200025 China

Background Auditing of surgical outcome is controversial due to lack of standard auditing system. POSSUM (Physiological and Operative Severity Score for the enUmeration of Morbidity and mortality)system provides a risk adjusted auditing in surgical practice, which is a reliable scoring system. However it has not been generalized in China, especially in gastric surgery. Present study evaluates the application of POSSUM system to compare surgical outcome of malignant gastric disease between specialized unit and general unit. Methods Retrospective study was performed on 394 patients who underwent surgical intervention for gastric cancer and malignant gastric lymphoma. POSSUM data were collected according to standard criteria described by its original authors. Exponential analysis method was used for data analysis. Observed to Expected morbidity (O:E) ratio was calculated for each unit to give risk adjusted comparison. All the complications were categorized into minor to severe to give an objective view of complications. Results There was significant difference in surgical outcome between specialized unit and general unit. POSSUM predicted morbidity well and O: E ratio of specialized unit was better than general unit. Further more postop stay wassignificantly shorter(P ＜0.001 ) in specialized unit and number of moderate and severe morbidity was significantly lower (P＜0.001) than general unit. Conclusions Surgical outcome of specialized unit was better than general unit. POSSUM can be used for risk adjusted auditing of postop complications in malignant gastric disease, which provides a reliable audit. However morbidity definition in POSSUM should be amended and modification in POSSUM formula may be necessary to fit major surgical interventions like gastric cancer surgery.

关键词：胃癌外科手术治疗并发症审核系统手术结局

Potential value of POSSUM scoring system in assessment of surgical outcome

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国际外科学杂志 2007年第9期34卷 644-648,F0003页

作者： birendra kumar sah ZHU Zheng-Gang General Surgery Department. Rui Jin Hospital Affiliated to Shanghai Jiao- tong University School of Medicine Shanghai 200025 China

The outcome of surgical intervention is not solely dependent on the abilities of the surgeon in isolation. The patient's physiological status, the disease that requires surgical correction, the nature of the operation... 详细信息

关键词：外科手术手术结局 POSSUM评分系统意义

Identification of EST–SSRs and molecular diversity analysis in Mentha piperita

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The Crop Journal 2015年第4期3卷 335-342页

作者： birendra kumar Umesh kumar Hemant kumar Yadav CSIR-Central Institute of Medicinal and Aromatic Plant Sciences P.O.CIMAP Near Kukrail Picnic Spot CSIR-National Botanical Research Institute Rana Pratap Marg Academy of Scientific and Innovative Research (AcSIR)

EST sequences of Mentha piperita available in the public domain(NCBI) were exploited to develop SSR markers. A total of 1316 ESTs were assembled into 155 contigs and 653 singletons and of these, 110 sequences were found to contain 130 SSRs, with a frequency of1 SSR/3.4 kb. Dinucleotide repeat SSRs were most frequent(72.3%) with the AG/CT(43.8%)repeat motif followed by AT/AT(16.2%). Primers were successfully designed for 68SSR-containing sequences(62.0%). The 68 primers amplified 13 accessions of M. piperita and 54 produced clear amplicons of the expected size. Of these 54, 33(61%) were found to be polymorphic among M. piperita accessions, showing from 2 to 4 alleles with an average of2.33 alleles/SSR, and the polymorphic information content(PIC) value varied between 0.13 and 0.51(average 0.25). All the amplified SSRs showed transferability among four different species of Mentha, with a highest in Mentha arvensis(87.0%) and minimum in Mentha citrata(37.0%). The newly developed SSRs markers were found to be useful for diversity analysis, as they successfully differentiated among species and accessions of Mentha.

关键词： EST–SSRs Genetic diversity Mentha piperita Polymorphic information content Transferability

Dietary supplementation of dried plum:a novel strategy to mitigate heat stress in broiler chickens

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Journal of Animal Science and Biotechnology 2021年第3期12卷 1056-1072页

作者： Sanjeev Wasti Nirvay sah Amit K.Singh Chin N.Lee Rajesh Jha birendra Mishra Department of Human Nutrition Food and Animal Sciences University of Hawaii at ManoaHonoluluHI 96822USA

Background:Heat stress is a significant problem in the poultry industry,causing a severe economic loss due to its detrimental effects on chickens’health and *** plum(DP)is a good source of minerals,vitamins,antioxidants,and phenolic *** have suggested that DP has several health benefits,such as maintaining the body’s redox system,immune status,and calcium *** on the health benefits of DP,we hypothesized that the dietary supplementation of DP would alleviate the detrimental effects of heat stress on broiler ***:To test the hypothesis,day-old broiler chicks(n=72)were randomly allocated to three treatment groups(n=24/group):no heat stress(NHS),heat stress(HS),and heat stress with dried plum(HS+DP),and reared under standard *** inclusion of 2.5%DP in the feed of the HS+DP group was made during the treatment period,while birds in other groups were provided with a standard finisher *** 21 days,birds in the HS and HS+DP groups were exposed to cyclic heat stress conditions(33℃ for 8 h during daytime)for 3 weeks,while those in the NHS group were reared under normal conditions(22-24℃).Weekly body weight and feed intake were recorded to calculate the average daily gain(ADG),average daily feed intake(ADFI),and feed conversion ratio(FCR).Heat stress significantly decreased the final body weight,ADG,ADFI,and increased FCR compared to the NHS group,whereas dietary supplementation of DP significantly improved these growth performance parameters compared to the HS ***,supplementation of DP significantly increased the expression of heat shock protein-related genes(HSF1,HSF3,HSP70,and HSP90),antioxidant-related genes(SOD1,SOD2,GPX1,GPX3,PRDX1,and TXN),tight junction-related genes(CLDN1,and OCLN),and immune-related genes(IL4,MUC2)in the ileum as compared to the HS *** microbiota analysis showed significant enrichment of Bacillales,Christensenellaceae,Bacillaceae,Peptostreptococcaceae,and Anaerot

关键词： Dried plum Gene expression Heat stress Microbiota Mitigation

Effects of in ovo delivered xylo-and mannan-oligosaccharides on growth performance,intestinal immunity,cecal short-chain fatty acids,and cecal microbiota of broilers

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Journal of Animal Science and Biotechnology 2022年第4期13卷 1099-1114页

作者： Amit kumar Singh Utsav Prakash Tiwari birendra Mishra Rajesh Jha Department of Human Nutrition Food and Animal SciencesUniversity of Hawaii at Manoa1955 East-West RdHonoluluHI 96822USA

Background:This study investigated a novel in ovo feeding strategy to determine the prebiotic effects of xylo-and mannan-oligosaccharides(XOS and MOS)differing in the degree of polymerization.A total of 192 fertilized eggs were divided into 6 treatment groups:i)normal saline control(NSC),ii)xylotriose(XOS3),iii)xylotetraose(XOS4),iv)mannotriose(MOS3),v)mannotetraose(MOS4),and vi)no injection control(NIC),each containing 4 replicate trays with 8 eggs per *** d 17 of incubation,3 mg of oligosaccharides(except for controls)dissolved in 0.5 mL of 0.85%normal saline were injected into the amnion of Cobb 500 broilers *** hatch,the chicks were raised for 28 d under standard husbandry practices and were fed a commercial broilers diet ad libitum,and samples were collected ***:The hatchability,growth performance,and relative weights of breast,drumstick,liver,and proventriculus were not different among the treatments(P>0.05).The XOS3 injection increased the total short-chain fatty acid production at d 28 compared with both control groups(P0.05).On the hatch day,the expression level of the CD3 gene(a T cell marker)was increased by XOS3,while the IL-10 gene(a marker of anti-inflammatory cytokine)was reduced by MOS4(P0.05),except XOS4 increased IL-4 compared with NSC(P<0.05).The broilers in the MOS4 group had higher operational taxonomic units(OTUs)and had more differentially abundant taxa,including order Lactobacillales and family Leuconostocaceae(P<0.05)than both controls on d *** predictive functional profiling indicated that the linoleic acid metabolism pathway w

关键词： Broilers Gut health Immunity in ovo Mannanoligosaccharides Microbiota Oligosaccharides Prebiotics Xylooligosaccharides

Phytohormones and their metabolic engineering for abiotic stress tolerance in crop plants

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The Crop Journal 2016年第3期4卷 162-176页

作者： Shabir H.Wani Vinay kumar Varsha Shriram Saroj kumar sah Division of Genetics and Plant Breeding Faculty of Agriculture WADURASher-e-Kashmir University of Agricultural Sciences and Technology Department of Biotechnology Modern College of ArtsScience and CommerceS.P.Pune University Department of Botany Prof.Ramkrishna More CollegeS.P.Pune University Department of Biochemistry Molecular BiologyEntomology and Plant PathologyMississippi State University

Abiotic stresses including drought,salinity,heat,cold,flooding,and ultraviolet radiation causes crop losses *** recent times,preventing these crop losses and producing more food and feed to meet the demands of ever-increasing human populations have gained unprecedented ***,the proportion of agricultural lands facing multiple abiotic stresses is expected only to rise under a changing global climate fueled by anthropogenic *** the mechanisms developed and deployed by plants to counteract abiotic stresses and maintain their growth and survival under harsh conditions thus holds great *** investigations have shown that phytohormones,including the classical auxins,cytokinins,ethylene,and gibberellins,and newer members including brassinosteroids,jasmonates,and strigolactones may prove to be important metabolic engineering targets for producing abiotic stress-tolerant crop *** this review,we summarize and critically assess the roles that phytohormones play in plant growth and development and abiotic stress tolerance,besides their engineering for conferring abiotic stress tolerance in transgenic *** also describe recent successes in identifying the roles of phytohormones under stressful *** conclude by describing the recent progress and future prospects including limitations and challenges of phytohormone engineering for inducing abiotic stress tolerance in crop plants.

关键词： Phytohormones Abiotic stress Metabolic engineering Plant stresses

Hill slope instability of Nainital City,Kumaun Lesser Himalaya,Uttarakhand,India

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Journal of Rock Mechanics and Geotechnical Engineering 2018年第2期10卷 280-289页

作者： Nirvan sah Mohit kumar Rajeev Upadhyay Som Dutt Department of Geology(CAS) Kumaun UniversityNainital263001India Wadia Institute of Himalayan Geology 33 General Mahadeo Singh RoadDehradun248001India

Nainital City of Kumaun Lesser Himalaya is prone to mass wasting processes during monsoon season,which mischievously triggers the hill slope instability in this region. Slate, dolomitic limestone, silty sandstone and rhythmite of the Krol Formation are the main rock types. The present study focuses on the investigation of slope stability in the region in terms of potential seismicity and landslide. Geological and geotechnical mapping indicates that the major portion of the area is characterized by slope wash materials and buildings. The combination of 3-4 joint sets with one random joint is the main structure at *** major geological structures of this area are Nainital lake fault passing from the center of the lake, Main Boundary Thrust at SW, and Khuriya Fault passing from the SE direction of Nainital City. This work finds that different types of discontinuities(e.g. joints and faults), overburden due to unplanned civil structures,and neotectonic activity in the vicinity of this ara affect the stability of the city. The slate forms the base of the city, dipping slightly towards the lake side along the NW direction, thus accelerating the instability of this area. Rock mass rating(RMR), slope mass rating, factor of safety(FOS) and graphical analysis of the discontinuity for slope kinematics indicate that the study area is a landslide-prone zone. This study can facilitate reducing the risk of human life, and contribute to the ongoing construction works in the area.

关键词： Hill slope instability Himalaya region Wedge failure Rock mass rating(RMR) Factor of safety(FOS)

Morphological phylogeny among 15 accessions of bamboos growing in Kokrajhar district of the Bodoland Territorial Area Districts,Assam

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Journal of Forestry Research 2018年第5期29卷 1379-1386页

作者： Anusriti Basumatary Sushil kumar Middha Talambedu Usha Samik Bhattacharya birendra kumar Brahma Arvind kumar Goyal Centre for Bamboo Studies and Department of Biotechnology Bodoland University DBT-BIF Centre Department of BiotechnologyPG Research CentreMaharani Lakshmi Ammanni College for Women Department of Biochemistry Maharani Lakshmi Ammanni College for Women Department of Molecular Ecology Max Planck Institute of Chemical Ecology

Bamboo is nature＇s wonderful gift historically associated with the rural population of the Bodoland Territorial Area ***,indiscriminate use of the resource and the ambiguity in the identification of bamboos in the absence of flowering characters pose a potential risk to the bamboo ***,we considered revising the taxonomic positions and phylogenetic relationships among 15 tropical bamboo species using 35 key morphological descriptors（MD） to assess the reliability of vegetative characters in identifying *** phylogenetic tree constructed from the similarity matrix,derived from an unweighted pair group method of analysis（UPGMA） of the 35 MDs,marginally deviated from the conventional taxonomic positions of the bamboo *** the two varieties,one forma of Bambusa vulgaris（***,***,***） and two species of the genus Dendrocalamus（*** and ***）clustered together in a single clade,and *** was distantly placed from their *** discrepancy with the conventional classification might have ensued since only vegetative characters such as culm anatomy,culm sheath,leaf,and leaf sheath were considered in the present *** the phylogenetic relationship among the 15 tropical bamboos（computed from exhaustive MDs）grossly corroborate the conventional taxonomic grouping,a molecular-marker-based multidisciplinary approach should resolve the remaining conflicts in bamboo identification.

关键词： Bamboo Bodoland Culm Culm-sheath Leaf-sheath Phylogenetic relationship conflict