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Regulation of Starch Biosynthesis in Sweet potato [Ipomoea batatas （L.） Lam.] Towards Starch Quality Improvement

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Regulation of Starch Biosynthesis in Sweet potato [Ipomoea b...

中国植物生理学会第十次会员代表大会暨全国学术年会

作者： Jun Yang and Peng Zhang* SIBS-ETH shanghai center for Cassava Biotechnology, National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology & Ecology, shanghai institutes for biological sciences, chinese academy of sciences, 300 Fenglin Road, shanghai 2.0032. China

＜正＞Granule-bound starch synthase I （GBSSI）, starch branching enzyme I （SBEI） and starch branching enzyme II （SBEII）, three key enzymes in starch biosynthesis, control starch composition in storage organs of p... 详细信息

关键词： sweet potato starch biosynthesis RNA interference genetic engineering

Experience-dependent visual feature extracting in Drosophila

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Experience-dependent visual feature extracting in Drosophila

The Third Asian Conference on Vision (ACV2.04)

作者： Yueqing Peng Wei Zhang & Aike Guo Institute of Neuroscience shanghai institutes for {2. sciences chinese academy of sciences 32. Yue-yang Road shanghai 2.0031 China. Laboratory of Visual Information Processing center for Brain and Cognitive sciences Institute of Biophysics chinese academy of sciences 15 Da-tun Road Chao-yang Dist. Beijing 100101 China

＜正＞ Visual feature extracting is defined as selecting main component from multiple visual features, such as color, shape, and so on. Certain kind of higher level cognitive processing is required to complete this task. The fruit flies are able to learn both color and shape cues. But we don’t know whether such a tiny brain can extract the most important cue in the case of complex feature combination. On the other hand, many cognitive processes can be modulated by

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Gene Expression Profile of Developing Cassava Storage Roots Revealed an Active Process of Glycolysis/Gluconeogenesis

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Gene Expression Profile of Developing Cassava Storage Roots ...

中国植物生理学会第十次会员代表大会暨全国学术年会

作者： Jun Yang, Dong An, Peng Zhang* SIBS-ETH shanghai center for Cassava Biotechnology, National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology & Ecology, shanghai institutes for biological sciences, chinese academy of sciences, 300 Fenglin Road, shanghai 2.0032. China

＜正＞Cassava storage root is a major sink for photosynthetic carbon assimilation. Its formation involves changes of transcriptional regulation and metabolic activities. For example, to accumulate starch in its parenc... 详细信息

关键词： cassava storage root development gene expression profile microarray

Gene expression and metabolite profiles of cotton fiber during cell elongation and secondary cell wall synthesis

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Cell Research 2007年第5期17卷 422-434页

作者： Jin-Ying Gou1,2.3 Ling-Jian Wang1,2.Shuang-Ping Chen4 Wen-Li Hu1,2.Xiao-Ya Chen1,2. 1 National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, shanghai institutes for {2. sciences, chinese academy of sciences, 300 Fenglin Road, shanghai 2.0032. China 2.CAS center of shanghai Chenshan Botanical Garden, 300 Fenglin Road, shanghai 2.0032. China 3 Graduate School of chinese academy of sciences, shanghai 2.0032. China 4 Department of Computer Science and Technology, University of Science and Technology of China, Hefei 2.002., China NationalKeyLaboratoryofPlantMolecularGenetics InstituteofPlantPhysiologyandEcologyShanghaiInstitutesforBiologicalSciencesChineseAcademyofSciences300FenglinRoadShanghai200032China

Cotton fibers elongate rapidly after initiation of elongation, eventually leading to the deposit of a large amount ofcellulose. To reveal features of cotton fiber cells at the fast elongation and the secondary cell wall synthesis stages, wecompared the respective transcriptomes and metabolite profiles. Comparative analysis of transcriptomes by cDNA arrayidentified 633 genes that were differentially regulated during fiber development. Principal component analysis (PCA)using expressed genes as variables divided fiber samples into four groups, which are diagnostic of developmental *** grouping results are also found if we use non-polar or polar metabolites as variables for PCA of developingfibers. Auxin signaling, wall-loosening and lipid metabolism are highly active during fiber elongation, whereas cellulosebiosynthesis is predominant and many other metabolic pathways are downregulated at the secondary cell wall synthesisstage. Transcript and metabolite profiles and enzyme activities are consistent in demonstrating a specialization process ofcotton fiber development toward cellulose synthesis. These data demonstrate that cotton fiber cell at a certain stage has itsown unique feature, and developmental stages of cotton fiber cells can be distinguished by their transcript and metaboliteprofiles. During the secondary cell wall synthesis stage, metabolic pathways are streamed into cellulose synthesis.

关键词： cotton fiber transcriptome metabolite profile auxin cell elongation cellulose synthesis

利用肠激酶加工融合蛋白的方法制备重组人甲状旁腺素(1～34)肽

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生物化学与生物物理学报 2002年第4期34卷 469-474页

作者：修朝阳周贺钺俞璎戴金凤陈常庆中国科学院上海生命科学研究院生物工程研究中心中国科学院上海生命科学研究院生物工程研究中心上海2.02.3

采用全基因分段合成和补丁连接相结合的方法构建了人甲状旁腺素 (hPTH) (1～ 34)肽基因 ,并利用GST融合表达系统 ,在大肠杆菌中克隆和可溶性表达了hPTH(1～ 34)肽基因。融合蛋白表达量占菌体总蛋白质的 2.5 %以上 ,经高密度发酵、亲和... 详细信息

采用全基因分段合成和补丁连接相结合的方法构建了人甲状旁腺素 (hPTH) (1～ 34)肽基因 ,并利用GST融合表达系统 ,在大肠杆菌中克隆和可溶性表达了hPTH(1～ 34)肽基因。融合蛋白表达量占菌体总蛋白质的 2.5 %以上 ,经高密度发酵、亲和纯化后 ,在每升发酵液中得到了 10 g融合蛋白。融合蛋白经肠激酶一步加工并亲合纯化和反相脱盐后 ,最终可以得到约 0 .6g /L人甲状旁腺素 (1～ 34)肽纯品 ,样品的理论回收值达到了4 8.75 %。产物的纯度和性质经HPLC、毛细管电泳、质谱分析、N端测序等得到证明 ,并与化学合成产物的结果相吻合。兔肾皮质细胞测活表明 ,重组产物与化学合成人甲状旁腺素 (1～ 34)肽具有相近的腺苷酸环化酶激活活性。

关键词：甲状旁腺素肠激酶脱水糜蛋白酶亲和柱亲和纯化

Nuclear reprogramming: the strategy used in normal development is also used in somatic cell nuclear transfer and parthenogenesis

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Cell Research 2007年第02期17卷 135-150页

作者： Tianlong Gao~(1,*) Junke Zheng~(2.*) Fengying Xing~(2.*) Haiyan Fang~2.Feng Sun~1 Ayong Yan~2.Xun Gong~2.Hui Ding~3 Fan Tang~2.Hui Z Sheng~2.~1Program for Graduation Studies,shanghai institutes for {2. sciences,chinese academy of sciences,shanghai 2.0031,China ~2.9} for Developmental Biology,Xinhua Hospital,shanghai Jiao Tong University,School of Medicine,1665 Kong Jiang Road,shanghai 2.0092.China ~3School of Animal Science and Technology,Yangzhou University,Yangzhou 2.5009,China 1Program for Graduation Studies Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China 2.0} for Developmental Biology Xinhua Hospital Shanghai Jiao Tong University School of Medicine 1665 Kong Jiang Road Shanghai 200092 China 2.0} for Developmental Biology Xinhua Hospital Shanghai Jiao Tong University School of Medicine 1665 Kong Jiang Road Shanghai 200092 China 2.0} for Developmental Biology Xinhua Hospital Shanghai Jiao Tong University School of Medicine 1665 Kong Jiang Road Shanghai 200092 China 1Program for Graduation Studies Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China 2.0} for Developmental Biology Xinhua Hospital Shanghai Jiao Tong University School of Medicine 1665 Kong Jiang Road Shanghai 200092 China 2.0} for Developmental Biology Xinhua Hospital Shanghai Jiao Tong University School of Medicine 1665 Kong Jiang Road Shanghai 200092 China 3School of Animal Science and Technology Yangzhou University Yangzhou 225009 China 2.0} for Developmental Biology Xinhua Hospital Shanghai Jiao Tong University School of Medicine 1665 Kong Jiang Road Shanghai 200092 China 2.0} for Developmental Biology Xinhua Hospital Shanghai Jiao Tong University School of Medicine 1665 Kong Jiang Road Shanghai 200092 China

Somatic cell nuclear transfer(SCNT)and parthenogenesis are alternative forms of reproduction and development,building new life cycles on differentiated somatic cell nuclei and duplicated maternal chromatin,*** thepreceding paper(Sun F,et al.,Cell Res 2.07;17:117-134.),we showed that an"erase-and-rebuild"strategy is used innormal development to transform the maternal gene expression profile to a zygotic ***,we investigate if the samestrategy also applies to SCNT and *** relationship between chromatin and chromatin factors(CFs)during SCNT and parthenogenesis was examined using immunochemical and GFP-fusion protein *** fromthese studies indicated that soon after nuclear transfer,a majority of CFs dissociated from somatic nuclei and were re-distributed to the cytoplasm of the *** erasure process in oogenesis is recaptured during the initial phase in *** CFs entered pseudo-pronuclei shortly after their *** parthenogenesis,all parthenogenotes underwentnormal oogenesis,and thus had removed most CFs from chromosomes before the initiation of *** CFswere subsequently re-associated with female pronuclei in time and sequence similar to that in fertilized *** these data,we conclude that the"erase-and-rebuild"process observed in normal development also occurs in SCNTand in parthenogenesis,albeit in altered *** process is responsible for transcription reprogramming in ***"erase"process in SCNT is compressed and the efficiency is compromised,which likely contribute tothe developmental defects often observed in nuclear transfer(nt)***,results from this study indicatedthat the cytoplasm of an egg contains most,if not all,essential components for assembling the zygotic program and canassemble them onto appropriate diploid chromatin of distinct origins.

关键词： nuclear reprogramming SCNT parthenogenesis chromatin factors chromatin embryogenesis

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HRCA技术在转基因植物检测中的应用

生物工程学报 2003年第3期19卷 294-300页

作者：陶震蔡兴锋颜志强胡小波杨胜利龚毅中国科学院上海生命科学研究院生物工程研究中心上海200233

超分支滚环扩增技术 (Hyperbranchedrollingcycleamplification ,HRCA)在近几年中逐渐引起人们的注意 ,并越来越多的用于基础研究和实际检测中。在本文中我们对该技术在转基因植物检测中的应用情况作了较全面的探索 ;根据 4种转基因植... 详细信息

超分支滚环扩增技术 (Hyperbranchedrollingcycleamplification ,HRCA)在近几年中逐渐引起人们的注意 ,并越来越多的用于基础研究和实际检测中。在本文中我们对该技术在转基因植物检测中的应用情况作了较全面的探索 ;根据 4种转基因植物中常用的外源基因或DNA片段设计了 4条锁式探针 (Padlockprobe) ,利用质粒pKK2.32. 8中的一段序列作为锁式探针中的共同连接部分 ,并根据该共同的连接部分序列设计一对通用的HRCA引物 ;利用同位素标记的锁式探针对HRCA反应中的连接一步的特异性研究表明 ,只有当锁式探针和相应的检测靶DNA同时存在于连接体系中时 ,锁式探针才能被有效地进行连接 ,从线性分子变为环型分子 ,在没有相应靶DNA存在时锁式探针仅以线性形式存在 ;连接时间的研究表明 ,如果所检测的靶DNA是质粒或较短的DNA片段时 ,较短的连接时间 (5～ 10min)就可以取得理想的最终检测效果 ,如果检测的靶DNA是复杂的植物基因组DNA时 ,连接时间需要较大程度的延长 (30～ 6 0min)才能取得理想的最终检测结果 ;HRCA的反应时间研究表明 ,较长的反应时间可以明显增加最终产物的量 ;对BstDNA聚合酶大片段酶用量的研究表明 ,在其它条件不变的情况下酶的用量可以在较大的范围内变化 (0 .5u～ 4u)而不影响最终检测结果 ;?

关键词： HRCA技术转基因植物检测锁式探针

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The Linear Plasmid SCP1 of Streptomyces coelicolor A3(2. has Three Replication Origins

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The Linear Plasmid SCP1 of Streptomyces coelicolor A3(2) has...

2.10年第四届全国微生物遗传学学术研讨会

作者： Ana Zeng,Li Zhong,Shiyuan Peng,Qiuxiang Cheng,Liqian Zhao and Zhongjun Qin~* Key Laboratory of Synthetic Biology,shanghai Institute of Plant Physiology and Ecology, shanghai {2. for biological sciences,the chinese academy of sciences,shanghai,China

Previous reports showed that the large linear plasmid SCP1 of Streptomyces coelieolor A3(2. contains a 5.4-kb locus near the center of the molecule capable of driving autonomous replication in circular mode in *** rep...

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中国神经科学学会第四次会员代表大会暨第七届全国学术会议

作者： Zhan-Tao BAI~3,Tong LIU~2.Xue-Yan PANG~1,Zhi-Fang CHAI~2.Jian SHI~2.Yong-Hua JI~1 1 School of life sciences,shanghai University,shanghai 2.0444,China 2.Graduate School of the chinese academy of sciences,Institute of Physiology,shanghai institutes for biological sciences, chinese academy of sciences,shanghai {12.,China. 3 pound College of life sciences,Yanan University,Shaanxi Engineering & Technological Research center for Conservation & Utilization of Regional biological Resources,Yanan 716000,China

＜正＞The experimental scorpion BmK（Buthus martensi Karsch） sting test has been demonstrated to be an available inflammatory animal pain model to understand the mechanisms of the tonic spontaneous responses,primary thermal hypersensitivity and mirror-imaged bilateral mechanical *** present study was designed further to investigate the contribution of BmK I,the main peptidergic component in BmK venom,to BmK sting-induced painrelated *** results showed that pain-related responses induced by the intraplantar injection of BmK I well mirrored the general characteristics of those by the experimental scorpion BmK *** subtle differences of BmK I test to BmK sting test were:1) BmK I-induced spontaneous behaviors showing an intermittent pattern with an occurrence of special paroxysmal-like behaviors;2. the duration and intensity of BmK I-induced paw edema（8-2. hours）,thermal hypersensitivity（3 days） and mirror-imaged bilateral hypersensitivity（1 week） was somewhat shorter or weaker than those of BmK *** investigation indicated that 1) BmK I-induced pain- related responses were sensitive to the systematical opioids（morphine）,local anesthisics（bupivacaine）,but not to anti-inflammatory drugs （indomethacin）;2. the development of thermal and mechanical hypersensitivity was relied earlier on the continuous afferent transmission,and then on a combined peripheral and central processing *** peak magnitudes of whole cell- and TTX-resistant sodium currents of rat small primary afferent neurons could be modulated by BmK I timedevelopmentally. These results strongly indicated that BmK I,a sodium channels-selective modulator,is one of the predominant contributors to the induction of pain-related responses by scorpion BmK *** I test is thus a simpler substitution for the experimental scorpion BmK sting,and BmK I may benefit as a probe to approach the sodium channels-involved mechanisms of the tissue injury-induced pain

关键词： Sodium channel modulators BmK I Paw inflammation Spontaneous pain Primary hypersensitivity Mirrorimage hypersensitivity