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Microbiomics and metabolomics insights into the microbial regulation on the formation of flavor components in the traditional fermentation process of Chinese Hongqu aged vinegar

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Food Science and Human Wellness 2024年第5期13卷 2765-2778页

作者： Shangong Tong Wenlong Li Yuandong rao Yanqin Xiao Yingyin Yan Weiling Guo Xucong Lü Jinyuan Sun Lianzhong Ai Li Ni Institute of Food Science and Technology College of Biological Science and EngineeringFuzhou UniversityFuzhou 350108China Food Nutrition and Health Research Center School of Advanced ManufacturingFuzhou UniversityJinjiang 362200China School of Food Science and Technology Jiangnan UniversityWuxi 214122China Beijing Laboratory for Food Quality and Safety Beijing Technology and Business UniversityBeijing 100048China School of Health Science and Engineering University of Shanghai for Science and TechnologyShanghai 200093China

This study aimed to investigate microbial succession and metabolic dynamics during the traditional fermentation of Hongqu aged vinegar,and explore the core functional microbes closely related to the formation of flavor *** analysis demonstrated that Lactobacillus,Acetobacter,Bacillus,Enterobacter,Lactococcus,Leuconostoc and Weissella were the predominant bacterial genera,while Aspergillus piperis,Aspergillus oryzae,Monascus purpureus,Candida athensensis,***,Penicillium ochrosalmoneum and Simplicillium aogashimaense were the predominant fungal *** analysis revealed that Acetobacter was positively correlated with the production of tetramethylpyrazine,acetoin and acetic acid,Lactococcus showed positive correlation with the production of 2-nonanone,2-heptanone,ethyl caprylate,ethyl caprate,1-hexanol,1-octanol and 1-octen-3-ol,*** and *** were positively associated with the production of diethyl malonate,2,3-butanediyl diacetate,acetoin,benzaldehyde and ***,non-volatile metabolites were also detected through ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry.A variety of amino acids and functional dipeptides were identified during the traditional brewing of Hongqu aged *** analysis revealed that Lactobacillus was significantly associated with DL-lactate,indolelactic acid,D-(+)-3-phenyllactic acid,pimelic acid,pregabalin and 3-aminobutanoic *** study is useful for understanding flavor formation mechanism and developing effective strategies for the suitable strains selection to improve the flavor quality of Hongqu aged vinegar.

关键词： Hongqu aged vinegar Traditional fermentation Microbial dynamics Flavor components Bidirectional orthogonal partial least squares

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Geochemical characteristics and origins of natural gases in the eastern Cote d'Ivoire Basin,West Africa

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Acta Oceanologica Sinica 2024年第08期 26-36页

作者： Li Li Quan Li Tao Cheng Songling Yang Yong rao Xinyu Liu Wenjing Ding CNOOC International Ltd. School of Natural Sciences Macquarie University CNOOC Research Institute

The gas sources in the eastern Cote d'Ivoire Basin（Tano Basin） are seldom reported and remain controversial due to multiple sets of potential source rocks and poorly documented geochemical characteristics of natural *** marine source rock potential from the Upper Albian to Turonian as well as the molecular composition and the stable carbon isotope composition of natural gases in the eastern Cote d'Ivoire Basin were studied in detail to investigate the origins of natural gases. The total organic carbon（TOC）, hydrogen index（HI）, and generation potential（S1+ S2） of source rocks indicate that both sapropelic source rocks and humic source rocks developed during the late Albian, whereas sapropelic source rocks developed during the Cenomanian and the Turonian. The normal order of δ13CH4< δ13C2H6< δ13C3H8(δ13C1< δ13C2< δ13C3), the relationship between C2/C3molar ratio and δ13C2-δ13C3, and the plot of δ13C1versus C1/（C2+C3） collectively show that the natural gases are thermogenic due to the primary cracking of kerogen, including the typical oil-associated gases from Well D-1, the mixed oil-associated gases and coal-derived gases from Well G-1 and Well L-1. Based on the plot of δ13C1versus δ13C2and the established relationship between δ13C1and equivalent vitrinite reflectance（Ro）, we proposed that the natural gases are in a mature stage（Ro generally varies from 1.0% to 1.3%）. Combined with results of basin modelling and oil-to-source correlation, the transitional to marine source rocks during the late Albian were thought to have made a great contribution to the natural gases. Our study will make a better understanding on petroleum system in the eastern Cote d'Ivoire Basin.

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Geochemical characteristics and origins of natural gases in the eastern Cote d’Ivoire Basin,West Africa

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Acta Oceanologica Sinica 2024年第8期43卷 26-36页

作者： Li Li Quan Li Tao Cheng Songling Yang Yong rao Xinyu Liu Wenjing Ding CNOOC International Ltd. Beijing 100028China School of Natural Sciences Macquarie UniversitySydneyNSW2109Australia CNOOC Research Institute Beijing 100028China

The gas sources in the eastern Cote d’Ivoire Basin(Tano Basin)are seldom reported and remain controversial due to multiple sets of potential source rocks and poorly documented geochemical characteristics of natural *** marine source rock potential from the Upper Albian to Turonian as well as the molecular composition and the stable carbon isotope composition of natural gases in the eastern Cote d’Ivoire Basin were studied in detail to investigate the origins of natural *** total organic carbon(TOC),hydrogen index(HI),and generation potential(S_(1)+S_(2))of source rocks indicate that both sapropelic source rocks and humic source rocks developed during the late Albian,whereas sapropelic source rocks developed during the Cenomanian and the *** normal order ofδ^(13)CH_(4)<δ^(13)C_(2)H6<δ^(13)C_(3)H_(8)(δ^(13)C_(1)<δ^(13)C_(2)<δ^(13)C_(3)),the relationship between C_(2)/C_(3)molar ratio andδ^(13)C_(2)-δ^(13)C_(3),and the plot ofδ^(13)C_(1)versus C_(1)/(C_(2)+C_(3))collectively show that the natural gases are thermogenic due to the primary cracking of kerogen,including the typical oil-associated gases from Well D-1,the mixed oil-associated gases and coal-derived gases from Well G-1 and Well *** on the plot ofδ^(13)C_(1)versusδ^(13)C_(2)and the established relationship betweenδ^(13)C_(1)and equivalent vitrinite reflectance(Ro),we proposed that the natural gases are in a mature stage(Ro generally varies from 1.0%to 1.3%).Combined with results of basin modelling and oil-to-source correlation,the transitional to marine source rocks during the late Albian were thought to have made a great contribution to the natural *** study will make a better understanding on petroleum system in the eastern Cote d’Ivoire Basin.

关键词： marine source rocks potential molecular composition stable carbon isotopes Upper Albian Tano Basin

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明代小说散韵结合体制的形成及诗歌叙事体性化

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南开学报（哲学社会科学版） 2024年第4期 47-59页

作者：饶龙隼四川大学中国俗文化研究所四川成都610207

唐宋以来各类小说的书面化助催散韵结合体制的形成,延至元明时期这种编创观览现象更加凸显并递有新变,从而形成明代小说独特的体制形态,因使其诗性叙事功能有所增强优化。明代小说中羼入的诗歌有多方面叙事功能,但在小说叙事中不占主导... 详细信息

唐宋以来各类小说的书面化助催散韵结合体制的形成,延至元明时期这种编创观览现象更加凸显并递有新变,从而形成明代小说独特的体制形态,因使其诗性叙事功能有所增强优化。明代小说中羼入的诗歌有多方面叙事功能,但在小说叙事中不占主导地位;若脱离散文叙事之主导,其诗性叙事就无所附丽。特别是在小说中羼入诗歌的增聚阶段,其叙事功能非但不能很好地得到发挥,反因非体性成分存在种种弊端。不过随着明代小说书面化,所羼诗歌进入体性化阶段;且因羼入诗歌体性化的增强,其诗性叙事完全融摄散文中。所谓体性化,是指羼入诗歌契入小说的故事情节,而与散文深度融合以修饰辅助叙事。

关键词：明代小说散韵结合体制书面化诗歌叙事体性化

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Overexpression of TRPV1 activates autophagy in human lens epithelial cells under hyperosmotic stress through Ca^(2+)-dependent AMPK/mTOR pathway

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International Journal of Ophthalmology(English edition) 2024年第3期17卷 420-434页

作者： Liu-Hui Huang Jiao Lyu Sheng Chen Ting-Yi Liang Yu-Qing rao Ping Fei Jing Li Hai-Ying Jin Pei-Quan Zhao Department of Ophthalmology Shanghai Xinhua Hospital Affiliated with Shanghai Jiao Tong University School of MedicineShanghai 200092China Department of Ophthalmology Shanghai Tenth People’s Hospital Affiliated with Shanghai Tongji University School of MedicineShanghai 200072China Centre for Eye Research Australia Royal Victorian Eye and Ear HospitalEast MelbourneVictoria 3002Australia Ophthalmology Department of SurgeryUniversity of MelbourneEast MelbourneVictoria 3002Australia Shenzhen Eye Hospital Shenzhen Key Laboratory of OphthalmologyAffiliated Shenzhen Eye Hospital of Jinan UniversityShenzhen 518040Guangdong ProvinceChina Department of Ophthalmology Shanghai East HospitalSchool of MedicineTongji UniversityShanghai 200120China

●AIM:To explore whether autophagy functions as a cellular adaptation mechanism in lens epithelial cells(LECs)under hyperosmotic stress.●METHODS:LECs were treated with hyperosmotic stress at the concentration of 270,300,400,500,or 600 mOsm for 6,12,18,24h in *** chain reaction(PCR)was employed for the mRNA expression of autophagyrelated genes,while Western blotting detected the targeted protein *** transfection of stub-RFP-sens-GFPLC3 autophagy-related double fluorescence lentivirus was conducted to detect the level of autophagy *** electron microscopy was used to detect the existence of *** interfering RNA of autophagy-related gene(ATG)7,transient receptor potential vanilloid(TRPV)1 overexpression plasmid,related agonists and inhibitors were employed to their influence on autophagy related *** cytometry was employed to test the apoptosis and intracellular Ca^(2+)*** membrane potential was measured by JC-1 *** cell counting kit-8 assay was used to calculate the cellular *** wound healing assay was used to evaluate the wound closure *** 6.0 software was utilized to evaluate the data.●RESULTS:The hyperosmotic stress activated autophagy in a pressure-and time-dependent manner in *** 1 protein expression and conversion of LC3B II to LC3B I increased,whereas sequestosome-1(SQSTM1)protein expression *** Ca^(2+)influx was stimulated caused by hyperosmotic stress,levels of mammalian target of rapamycin(mTOR)phosphorylation decreased,and the level of AMP-activated protein kinase(AMPK)phosphorylation increased in the early *** on this evidence,autophagy activation through the Ca^(2+)-dependent AMPK/mTOR pathway might represent an adaptation process in LECs under hyperosmotic *** stress decreased cellular viability and accelerated apoptosis in LECs and cellular migration *** of autophagy by ATG7 knockdown had sim

关键词： cataract posterior capsular opacification lens epithelial cell hyperosmotic stress autophagy apoptosis transient receptor potential vanilloid 1

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Immunology reshapes neuroscience,and neuroscience reshapes immunology

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Fundamental Research 2024年第2期4卷 199-200页

作者： Bo Peng Yanxia rao Yun Wang Shumin Duan Hai Qi Jing Yang Hongliang Zhang Department of Neurosurgery Huashan HospitalInstitute for Translational Brain ResearchState Key Laboratory of Medical NeurobiologyMOE Frontiers Center for Brain ScienceMOE Innovative Center for New Drug Development of Immune Inflammatory DiseasesFudan UniversityShanghai 200040China Department of Laboratory Animal Science MOE Frontiers Center for Brain ScienceFudan UniversityShanghai 200032China Neuroscience Research Institute and Department of Neurobiology School of Basic Medical SciencesKey Laboratory for NeuroscienceMinistry of Education/National Health Commission and State Key Laboratory of Natural and Biomimetic DrugsPeking UniversityBeijing 100083China PKU-IDG/McGovern Institute for Brain Research Peking UniversityBeijing 100871China Faculty of Medicine and Pharmaceutical Sciences Zhejiang UniversityHangzhou 310014China School of Medicine Tsinghua UniversityBeijing 100084China IDG/McGovern Institute for Brain Research Center for Life SciencesSchool of Life SciencesPeking UniversityBeijing 100871China Chinese Institute for Brain Research Beijing 102206China Department of Life Sciences National Natural Science Foundation of ChinaBeijing 100085China

As a result of evolution,our body has evolved into a sophisticated system in which various tissues and organs cooperate in a highly orchestrated *** tissues or organs operate in *** brain serves as the command center of our body and is relatively isolated from the peripheral system due to the presence of the blood-brain barrier(BBB).This barrier prevents direct and extensive interaction between blood cells,plasma molecules,and brain cells,contributing to the longstanding perception of the brain as an“immune-privileged”***,neuroscientists and immunologists historically conducted separate research with minimal overlap between these two ***,this perspective has undergone reconsideration over the past few decades.

关键词： organs overlap contributing

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Analysis of Influencing Factors on Fruit Consumption of Urban and Rural Residents in Beijing

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Asian Agricultural Research 2024年第6期16卷 1-4,10页

作者： Zihao WANG rao CHEN Weimin YANG College of Economics and Management Beijing University of AgricultureBeijing 102206China Beijing Research Center of Rural Revitalization Beijing 102206China Department of Basic Education Beijing University of AgricultureBeijing 102206China

In light of the rapid expansion of China s economy,there has been a notable shift in the consumption patterns of urban and rural *** is evident in the increased consumption of agricultural products,with fruit consumption representing a particularly pronounced *** 2023 Beijing Consumption Statistics indicate that demand for fruit is increasing,accompanied by heightened competition for quality and *** paper is based on research that examines the current situation of fruit consumption among urban and rural residents in *** results indicate that when urban and rural residents in Beijing purchase fruits,price is the most significant factor influencing fruit ***,appearance,and variety are also considered to be important influencing factors,while the influence of factors such as fruit type,consumption pattern,and fruit brand is relatively minor.

关键词： Urban and rural residents of Beijing Fruit consumption Influencing factor Consumption behavior

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Wild Nutrition’s Food-Grown® Magnesium Supplementation Increases Sleep Quality and Sleep Duration and Reduces Stress in a Healthy Adult Population: A Double-Blind, Randomised, Placebo-Controlled Study

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Food and Nutrition Sciences 2024年第7期15卷 509-523页

作者： David Briskey Jane Erickson Chelsie Smith Amanda rao School of Human Movement and Nutrition Sciences University of Queensland Brisbane Australia RDC Clinical Brisbane Australia

Background: Magnesium, an essential mineral crucial for various bodily functions, has been shown to positively influence sleep patterns. This study aimed to evaluate the efficacy of Food-Grown® magnesium in enhancing sleep quality and duration, as well as overall well-being. Methods: Eighty participants were randomly assigned to receive either 80 mg of Food-Grown® magnesium or a placebo (microcrystalline cellulose) daily for 8 weeks. Participants completed questionnaires assessing sleep quality, daytime drowsiness, quality of life, anxiety, and stress levels. Additionally, participants maintained daily sleep diaries and wore wrist-worn actigraphy devices. The primary outcome measured was the change in sleep quality and duration. Results: Seventy-one participants fulfilled all study requirements (35 in the active group and 36 in the placebo group). Magnesium supplementation significantly improved reported sleep quality, with the active group showing a 32% increase compared to 16% in the placebo group (p = 0.034). Moreover, magnesium supplementation led to a decrease in reported stress scores at week 8 compared to the placebo group (3.7 ± 2.6 vs. 5.5 ± 3.1, respectively). Both the magnesium and placebo groups exhibited significant increases in reported sleep duration and reductions in time to fall asleep, sleep disturbance, sleep latency, sleep medication usage, and total Pittsburgh Sleep Quality Index score at week 8 compared to baseline. Conclusion: Magnesium supplementation notably enhanced sleep quality and reduced stress levels compared to the placebo group. These findings highlight the potential of magnesium as a beneficial supplement for improving sleep quality and overall well-being.

关键词： Magnesium Sleep Quality Sleep Duration Stress Food-Grown

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Effect of β-Glucan (Angel Yeast) Compared to a Placebo on Cold and Flu Incidence and Symptoms in an Adult Population—A Double Blind, Randomised Controlled Trial

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Food and Nutrition Sciences 2024年第6期15卷 484-497页

作者： David Briskey Haibo Zhang Zhixian Chen Amanda rao RDC Clinical Brisbane Australia School of Health Science and Engineering Shanghai Engineering Research Center of Food Microbiology University of Shanghai for Science and Technology Shanghai China The Hubei Provincial Key Laboratory of Yeast Function Angel Yeast Co. Ltd. Yichang China

Background: 1-3, 1-6 β-glucan derived from Baker’s yeast (Saccharomyces cerevisiae) has been widely studied for its immune stimulatory capabilities and safety. Previous studies found β-glucan to have efficacy at reducing incidence of URTIs as well as being a low risk for negative side effects. The current study aimed to examine the effects of yeast β-glucan (Angel Yeast) on cold and flu incidences and symptoms in healthy adults. Methods: Two hundred and thirty-one males and females aged 18 to 65 years old supplemented with either β-glucan or a placebo for 3-months. Participants completed a general health questionnaire every 4 weeks and in addition, if participants experienced any cold or flu symptoms, these were recorded daily (along with severity) until resolved or up to 2 weeks. Results: Supplementation with β-glucan reduced the self-reported severity of sore throats and improved sleep quality compared to the placebo group. Conclusions: Yeast β-glucan supplementation appears to be able to help reduce certain symptoms experienced during a cold or flu episode and is safe and well tolerated.

关键词： Beta-Glucan Cold Flu Baker’s Yeast

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The Effect of External Pressure on Mechanical Properties of Aquamarine Gemstone Using First Principles Studies

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Journal of Minerals and Materials Characterization and Engineering 2024年第5期12卷 237-246页

作者： Evarist Kahuluda Pulapa Ventkata Kanaka rao Stanley Mwanga Department of Physics College of Natural and Mathematical Sciences University of Dodoma Dodoma United Republic of Tanzania

Aquamarine gemstones are popular jewelry in the gemstone trade and are currently one of the important products in the world market because of their economic value. Aquamarine is a Beryllium Aluminium Silicate with the chemical formula Be3Al2Si6O18 and crystallizes in the hexagonal system with space group P6/mcc (192), and Tanzania has wide deposits of aquamarine gemstones. The quality of gemstone depends on its characteristic properties, including electronic, optical, and mechanical properties. In the present study, the effect of external pressure on mechanical properties including independent elastic constants and other related parameters such as Bulk modulus, Shear modulus, Young modulus, Poisson’s ratio, and Compressibility were studied. Density Function Theory in the forcite module of the material studies software on the external pressure within the range of 0 - 200 GPa on the optimized structure at electrostatic, Van der Waals and Ewald terms were used in this study. The results reveal that the independent elastic constants are mechanically unstable at 50 - 120 Gpa and are stable at 0 - 40 GPa and above 120 GPa, with the average bulk modulus, shear modulus, young modulus, Poisson’s ratio of 2319.9447, 652.3058, 1789.2236, and 0.26 respectively with the compressibility of 0.059921/TPa, this indicates that aquamarine gemstones are stable against strain and strongly against shear stress but opposing shear deformation. These values are within other crystalline materials found in the literature. This provides technological backing for the comprehensive valuation of mechanical properties, quality, and stability of gemstones available in Tanzania.

关键词： Aquamarine Gemstones Mechanical Properties External Pressure Biovia Material Studio Forcite Module

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