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Bioactive Materials 2023年第7期25卷 677-688页

作者： Lei Zhang bingjin wang Bo Song Yonggang Yao Seung-Kyum Choi Cao Yang Yusheng Shi State Key Laboratory of Material Processing and Die&Mould Technology School of Materials Science and EngineeringHuazhong University of Science and TechnologyWuhan430074China Department of Orthopaedics Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022China George W.Woodruff School of Mechanical Engineering Georgia Institute of TechnologyAtlantaGA30332USA

Biomimetic metallic biomaterials prepared for bone scaffolds have drawn more and more attention in recent ***,the topological design of scaffolds is critical to cater to multi-physical requirements for efficient cell seeding and bone regeneration,yet remains a big scientific challenge owing to the coupling of mechanical and mass-transport properties in conventional scaffolds that lead to poor control towards favorable modulus and permeability ***,inspired by the microstructure of natural sea urchin spines,biomimetic scaffolds constructed by pentamode metamaterials(PMs)with hierarchical structural tunability were additively manufactured via selective laser *** mechanical and mass-transport properties of scaffolds could be simultaneously tuned by the graded porosity(B/T ratio)and the tapering level(D/d ratio).Compared with traditional metallic biomaterials,our biomimetic PM scaffolds possess graded pore distribution,suitable strength,and significant improvements to cell seeding efficiency,permeability,and impact-tolerant capacity,and they also promote in vivo osteogenesis,indicating promising application for cell proliferation and bone regeneration using a structural innovation.

关键词： Biomimetic biomaterials Pentamode metamaterials Selective laser melting Mass-transport properties Bone scaffolds

Small extracellular vesicles with nanomorphology memory promote osteogenesis

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Bioactive Materials 2022年第11期7卷 425-438页

作者： Liang Ma Wencan Ke Zhiwei Liao Xiaobo Feng Jie Lei Kun wang bingjin wang Gaocai Li Rongjin Luo Yunsong Shi Weifeng Zhang Yu Song Weibin Sheng Cao Yang Department of Orthopaedics Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430022China Department of Spine Surgery The First Affiliated Hospital of Xinjiang Medical UniversityUrumqi830054China

Nanotopographical cues endow biomaterials the ability to guide cell adhesion, proliferation, and differentiation. Cellular mechanical memory can maintain the cell status by retaining cellular information obtained from past mechanical microenvironments. Here, we propose a new concept “morphology memory of small extracellular vesicles (sEV)” for bone regeneration. We performed nanotopography on titanium plates through alkali and heat (Ti8) treatment to promote human mesenchymal stem cell (hMSC) differentiation. Next, we extracted the sEVs from the hMSC, which were cultured on the nanotopographical Ti plates for 21 days (Ti8-21-sEV). We demonstrated that Ti8-21-sEV had superior pro-osteogenesis ability in vitro and in vivo. RNA sequencing further confirmed that Ti8-21-sEV promote bone regeneration through osteogenic-related pathways, including the PI3K-AKT signaling pathway, MAPK signaling pathway, focal adhesion, and extracellular matrix-receptor interaction. Finally, we decorated the Ti8-21-sEV on a 3D printed porous polyetheretherketone scaffold. The femoral condyle defect model of rabbits was used to demonstrate that Ti8-21-sEV had the best bone ingrowth. In summary, our study demonstrated that the Ti8-21-sEV have memory function by copying the pro-osteogenesis information from the nanotopography. We expect that our study will encourage the discovery of other sEV with morphology memory for tissue regeneration.

关键词： Nanotopographic PEEK hMSCs Small extracellular vesicles Osteogenesis

Ultrasound-assisted natural deep eutectic solvents extraction of podophyllotoxins from Juniperus sabina L:optimization,enrichment,and anti-drug resistant bacteria activity

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Ultrasound-assisted natural deep eutectic solvents extractio...

陕西省植物学会2024年学术研讨会

作者： Xinru Li Dongmei wang bingjin wang Dengwu Li College of Forestry Northwest A&F UniversityShaanxi Key Laboratory of Economic Plant Resources Development and Utilization

Podophyllotoxin,along with its congeners and derivatives exhibit strong biological activity mainly as antitumor drugs and antiviral ***,the ultrasound-assisted extraction of podophyllotoxins including α-peltatin,podophyllotoxin,podophyllotoxinone and deoxypodophyllotoxin from Juniperus sabina *** using natural deep eutectic solvents(NADESs) were developed first *** simultaneously and effectively extract 4 target podophyllotoxins,19 NADESs were screened to determine the best solvent to extract *** chloride-lactic acid(1:1) with 30% water content was proved to have the best extraction *** optimal extraction process with NADES were NADES/solid ratio 30 mL/g,reaction temperature 44℃,time 53 min and ultrasound power 495 *** optimized extraction conditions,the total podophyllotoxins extraction content was 27.520 mg/g,196.84% higher than that using traditional methanol *** content of α-peltatin,podophyllotoxin,deoxypodophyllotoxin and podophyllotoxinone were 13.567,9.548,2.021,2.204 mg/g,***,the podophyllotoxins were effectively isolated from the natural deep eutectic solvent using D101 macroporous *** optimal conditions for enriching target podophyllotoxins by D101 macroporous resin were 30 mg/mL *** leaves crude extract solution with 5 bed volume sample volume,and elution with 6 BV of80% ***,a scale-up enrichment experiment of podophyllotoxins was performed under optimum procedure,and the results showed that the target podophyllotoxins content was increased by 8.92 times,with recoveries in the range of75.86 to 85.69%.Additionally,the enriched podophyllotoxins effectively restored the antibacterial efficacy of cefazolin against methicillin-resistant Staphylococcus aureus and colistin-resistant Escherichia coli,resulting in 16-64-fold reduction in ***,the method developed in this study could be an eco-friendly,efficient and sustainable alternative for the select

关键词： Natural deep eutectic solvent Podophyllotoxins Juniperus sabina L. Macroporous resin Antibacterial activity

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矿热炉电极调节PID控制系统设计

铁合金 2009年第1期40卷 38-40页

作者：王炳金云南文山斗南锰业股份有限公司文山中国663101

阐述了矿热炉电极调节PID控制系统的设计,将其非线性系统的控制转变成分段线性化系统控制,并着重阐述了每个控制环节的设计与工作原理。

关键词：电极 PID 控制器系统

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PLC可编程控制系统干扰问题的解决方案

铁合金 2009年第4期40卷 46-48页

作者：王炳金云南文山斗南锰业股份有限公司文山中国663101

阐述了PLC可编程控制系统干扰问题的解决方案,从抗干扰的设计、采取的措施等多方面介绍了处理干扰的途径,对实践具有指导意义。

关键词： PLC 干扰控制系统

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矿热炉炉顶布料控制系统的设计与实践

铁合金 2006年第6期37卷 43-47页

作者：谢子洪汤顺祥段文成王炳金云南文山斗南锰业有限责任公司铁合金厂文山中国663101

介绍了通过运用PLC和触摸屏技术来设计矿热炉炉顶布料控制系统,并针对该套控制系统的设计、外围电气设备的电控原理,控制系统中的触摸屏页面设计和PLC的原理及作用进行了分析,并通过在12．5 MVA矿热炉上的运用,取得了显著的效果。

关键词： PLC触摸屏布料系统控制系统

促进或抑制ACE2-Ang(1-7)-MasR轴活化对大鼠心肺复苏后多器官功能不全的影响

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实用医学杂志 2019年第12期35卷 1915-1919页

作者：袁琦松朱丽王炳今李岚黄佳周霞周厚荣贵州省人民医院全科医学教研室

目的探讨ACE2-Ang(1-7)-MasR轴在大鼠心脏骤停-心肺复苏-自主循环恢复后(CA-CPRROSC)心、肝、肾功能损伤中的作用,为提高ROSC后患者生存率提供实验依据。方法建立CA-CPR模型,随机将大鼠分为假手术组、常规复苏组、Ang(1-7)组及A779组,... 详细信息

目的探讨ACE2-Ang(1-7)-MasR轴在大鼠心脏骤停-心肺复苏-自主循环恢复后(CA-CPRROSC)心、肝、肾功能损伤中的作用,为提高ROSC后患者生存率提供实验依据。方法建立CA-CPR模型,随机将大鼠分为假手术组、常规复苏组、Ang(1-7)组及A779组,监测各组血流动力学指标、生化指标、心肌病理性损伤情况。结果与假手术组比较,其余3组心率(HR)、平均动脉压(MAP)、±LVdp/dtmax下降(P <0.05),ROSC后6 h的BNP、cTnT、Cr、ALT升高(P <0.05)。与常规复苏组相比,Ang(1-7)组各时点±LVdp/dtmax增高,ROSC后6 h的BNP、cTnT均降低(P <0.05),而A779组则相反。结论大鼠在CA-CPRROSC后的心、肝、肾功能出现一定程度的损害,促进该轴活化参与大鼠心肺复苏后器官功能的保护作用。

关键词：心脏骤停大鼠 Ang(1-7) 多器官功能损伤