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Quantitative proteomics analysis of parthenogenetically induced pluripotent stem cells

Protein & Cell 2011年第8期2卷 631-646页

作者： Zhe Hu LeiWang Zhensheng Xie Xinlei Zhang Du Feng Fang Wang bingfeng zuo Lingling Wang Zhong Liu Zhisheng Chen Fuquan Yang Lin Liu Department of Cell Biology and Genetics Key Laboratory of Bioactive Materials of Ministry of EducationCollege of Life SciencesNankai UniversityTianjin 300071China Laboratory of Proteomics Institute of BiophysicsChinese Academy of SciencesBeijing 100101China Center for Computational and Systems Biology Institute of BiophysicsChinese Academy of SciencesBeijing 100101China

Parthenogenetic embryonic stem(pES)cells isolated from parthenogenetic activation of oocytes and embryos,also called parthenogenetically induced pluripotent stem cells,exhibit pluripotency evidenced by both in vitro and in vivo differentiation *** proteomic analysis was performed using differential in-gel electrophoresis and isotope-coded affinity tag-based quantitative proteomics to investigate the molecular mechanisms underlying the developmental pluripotency of pES cells and to compare the protein expression of pES cells generated from either the in vivo-matured ovulated(IVO)oocytes or from the in vitro-matured(IVM)oocytes with that of fertilized embryonic stem(fES)cells derived from fertilized embryos.A total of 76 proteins were upregulated and 16 proteins were downregulated in the IVM pES cells,whereas 91 proteins were upregulated and 9 were downregulated in the IVO pES cells based on a minimal 1.5-fold change as the cutoff *** distinct pathways were found in the differentially expressed proteins except for those involved in metabolism and physiological ***,no differences were found in the protein expression of imprinted genes between the pES and fES cells,suggesting that genomic imprinting can be corrected in the pES cells at least at the early *** germline competent IVM pES cells may be applicable for germ cell renewal in aging ovaries if oocytes are retrieved at a younger age.

关键词： parthenogenetic embryonic stem cell proteome fluorescent two-dimensional difference in-gel electrophoresis isotope-coded affinity tag

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Personalized immunotherapy for hepatocellular carcinoma with universal exosome vaccines that engage adaptive and innate immune responses

Personalized immunotherapy for hepatocellular carcinoma with...

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第十四届全国免疫学学术大会

作者： bingfeng zuo Yang Zhang Kangjie Zhao Haifang Yin Tianjin Medical University

Personalized immunotherapy utilizing cancer vaccines tailored to the tumors of individual patients holds promise for tumors with high genetic ***,we demonstrate a general strategy for biological nanovaccines that trigger tailored tumor-specific immune responses for hepatocellular carcinoma(HCC).Dendritic cell(DC)-derived exosomes(DEX)are painted with a HCC-targeting peptide(P47-P),an a-fetoprotein epitope(AFP212-A2)and a functional domain of high mobility group nucleosome-binding protein 1(N1 ND-N),an immunoadjuvant for DC recruitment and activation,via an exosomal anchor peptide to form a "trigger" DEX vaccine(DEXP&A2&N).DEXP&A2&N specifically promoted recruitment,accumulation and activation of CD103DC in mice with orthotopic HCC tumor,resulting in enhanced cross-presentation of tumor neoantigens and de novo T cell response,elicited significant tumor retardation and tumor-specific immune responses in HCC mice with large tumor *** results demonstrate the capacity of a universal DEX vaccine that trigger targeted response towards genetically heterogeneous tumors via innate and adaptive immunity pathways to eradicate tumors,and thus offer a generalizable approach for therapeutic cancer vaccines.

关键词： exosome hepatocellular carcinoma personalized immunotherapy adaptive and innate immunity

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关于房价上涨深层原因及对策的思考

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铜陵职业技术学院学报 2010年第2期9卷 23-24页

作者：左斌峰铜陵职业技术学院安徽铜陵244000

过去5年,在国家房地产宏观调控下,房价一路上涨,目前房价处于高位运行。文章从土地制度、供求关系、住房保障制度、房地产法制等方面探析房价上涨的深层原因,并就抑制房价提出几点对策建议。

关键词：房价上涨原因对策

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Airborne fungal communities are more susceptible to anthropogenic activities than bacteria

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Journal of Environmental Sciences 2025年第3期149卷 564-573页

作者： Yuanyuan Pan Zhiwei zuo Xueyun Huang Renguo Zhu School of Water Resources&Environmental Engineering East China University of TechnologyNanchang 330013China Jiangxi Province Key Laboratory of the Causes and Control of Atmospheric Pollution East China University of TechnologyNanchang 330013China

Airborne microorganisms(AM)have significant environmental and health *** studies have been conducted to investigate the factors influencing the composition and diversity of ***,the knowledge of AM with anthropogenic activities has not reach a *** this study,we took advantage of the dramatic decline of outdoor anthropogenic activities resulting from COVID-19 lockdown to reveal their *** collected airborne particulate matter before and during the lockdown period in two *** results showed that it was fungal diversity and communities but not bacteria obviously different between pre-lockdownand lockdown samples,suggesting that airborne fungiwere more susceptible to anthropogenic activities than ***,after the implementation of lockdown,the co-occurrence networks of both bacterial and fungal community became more complex,whichmight be due to the variation of microbial ***,Mantel test and correlation analysis showed that air pollutants also partly contributed to microbial *** fungal community was more affected by air pollutants than bacterial ***,some human pathogens like Nigrospora and Arthrinium were negatively correlated with air ***,our study highlighted the more impacts of anthropogenic activities on airborne fungal community than bacterial community and advanced the understanding of associations between anthropogenic activities and AM.

关键词： COVID-19 lockdown Anthropogenic activities Airborne microorganisms Response Air pollutants

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Smaller, faster, lower-power analog RRAM matrix computing circuits without performance compromise

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Science China(Information Sciences) 2025年第2期68卷 293-306页

作者： Yubiao LUO Pushen zuo Shiqing WANG Zhong SUN Ru HUANG School of Integrated Circuits Peking University Institute for Artificial Intelligence Peking University Beijing Advanced Innovation Center for Integrated Circuits

Recently, the analog matrix computing(AMC) concept has been proposed for fast, efficient matrix operations, by configuring global feedback loops with crosspoint resistive memory arrays and operational amplifiers(OAs). The implementation of a general real-valued matrix(containing both positive and negative elements) is enabled by using a set of analog inverters,which, however, is considered inefficient regarding circuit compactness, power consumption, and temporal response. Here, with the assistance of the conductance compensation(CC) strategy to take full advantage of the inherent differential inputs of OAs,new AMC circuits without analog inverters are designed. Such a design saves the area occupation and power dissipation of analog inverters, and thus turns to be smaller and lower-power. Simulation results reveal that the new circuit also shows a faster response towards the steady state, thanks to the reduction of poles in the circuit, which, again, is contributed by the elimination of analog inverters. Along with all of these benefits, extensive simulations demonstrate that the CC-AMC circuits do not compromise the computing performance in terms of relative error caused by various non-ideal factors in the circuit.

关键词： analog computing in-memory computing matrix resistive memory conductance compensation

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Efficient implementation of quantum permutation algorithm using a polar SrO molecule in pendular states

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Communications in Theoretical Physics 2025年第2期77卷 41-53页

作者： Jie-Ru Hu zuo-Yuan Zhang Jin-Ming Liu State Key Laboratory of Precision Spectroscopy School of Physics and Electronic Science East China Normal University School of Physical Science and Technology Yangzhou University Chongqing Key Laboratory of Precision Optics Chongqing Institute of East China Normal University

Quantum algorithms offer more enhanced computational efficiency in comparison to their classical counterparts when solving specific tasks. In this study, we implement the quantum permutation algorithm utilizing a polar molecule within an external electric field. The selection of the molecular qutrit involves the utilization of field-dressed states generated through the pendular modes of SrO. Through the application of multi-target optimal control theory, we strategically design microwave pulses to execute logical operations, including Fourier transform,oracle Ufoperation, and inverse Fourier transform within a three-level molecular qutrit *** observed high fidelity of our outcomes is intricately linked to the concept of the quantum speed limit, which quantifies the maximum speed of quantum state manipulation. Subsequently,we design the optimized pulse sequence to successfully simulate the quantum permutation algorithm on a single SrO molecule, achieving remarkable fidelity. Consequently, a quantum circuit comprising a single qutrit suffices to determine permutation parity with just a single function evaluation. Therefore, our results indicate that the optimal control theory can be well applied to the quantum computation of polar molecular systems.

关键词： polar molecule optimal control quantum permutation algorithm pendular states

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Vector meson’s spin alignments in high energy reactions

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Science China(Physics,Mechanics & Astronomy) 2025年第1期68卷 1-16页

作者： Jin-Hui Chen zuo-Tang Liang Yu-Gang Ma Xin-Li Sheng Qun Wang Key Laboratory of Nuclear Physics and Ion-beam Application(MoE) Institute of Modern PhysicsFudan UniversityShanghai 200433China Institute of Frontier and Interdisciplinary Science Key Laboratory of Particle Physics and Particle Irradiation(MOE)Shandong UniversityQingdao 266237China INFN-Firenze Sesto Fiorentino FI 50019Italy Department of Modern Physics University of Science and Technology of ChinaHefei 230026China School of Mechanics and Physics Anhui University of Science and TechnologyHuainan 232001China

The global spin alignment of vector mesons has been observed by the STAR collaboration at the Relativistic Heavy Ion Collider(RHIC)at Brookhaven National Laboratory(BNL).It provides a unique opportunity to probe the correlation between the po-larized quark and antiquark in the strongly coupled quark-gluon plasma(sQGP)produced in relativistic heavy ion collisions,opening a new window to explore the properties of *** addition,spin alignments of vector mesons have also been observed in other high-energy particle collisions such as e^(+)e^(−)annihilations at high energies where hadron production is dominated by quark fragmentation *** results obtained are quite different from those obtained in heavy ion collisions where quark coalescence/combination mechanism dominates suggesting strong dependence on hadronization *** comprehensive studies in different hadronization processes are *** this article,we present a brief review of theoretical and experimen-tal advances in the study of vector meson’s spin alignments in a variety of high-energy particle collisions,with emphasis on hadronization mechanisms.

关键词： vector meson spin alignment global polarization relativistic heavy ion collisions fragmentation

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Microstructure effect of mechanical and cracking behaviors on brittle rocks using image-based fast Fourier transform method

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Journal of Rock Mechanics and Geotechnical Engineering 2025年第1期17卷 399-413页

作者： Mingyao Li Lei Peng Dejun Liu Jianping zuo School of Mechanics and Civil Engineering China University of Mining and TechnologyBeijing100083China State Key Laboratory for Tunnel Engineering China University of Mining and TechnologyBeijing100083China

The internal microstructures of rock materials, including mineral heterogeneity and intrinsic microdefects, exert a significant influence on their nonlinear mechanical and cracking behaviors. It is of great significance to accurately characterize the actual microstructures and their influence on stress and damage evolution inside the rocks. In this study, an image-based fast Fourier transform (FFT) method is developed for reconstructing the actual rock microstructures by combining it with the digital image processing (DIP) technique. A series of experimental investigations were conducted to acquire information regarding the actual microstructure and the mechanical properties. Based on these experimental evidences, the processed microstructure information, in conjunction with the proposed micromechanical model, is incorporated into the numerical calculation. The proposed image-based FFT method was firstly validated through uniaxial compression tests. Subsequently, it was employed to predict and analyze the influence of microstructure on macroscopic mechanical behaviors, local stress distribution and the internal crack evolution process in brittle rocks. The distribution of feldspar is considerably more heterogeneous and scattered than that of quartz, which results in a greater propensity for the formation of cracks in feldspar. It is observed that initial cracks and new cracks, including intragranular and boundary ones, ultimately coalesce and connect as the primary through cracks, which are predominantly distributed along the boundary of the feldspar. This phenomenon is also predicted by the proposed numerical method. The results indicate that the proposed numerical method provides an effective approach for analyzing, understanding and predicting the nonlinear mechanical and cracking behaviors of brittle rocks by taking into account the actual microstructure characteristics.

关键词： Rock microstructure Cracking process Brittle rocks Fast Fourier transform(FFT) Digital image processing(DIP)

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Achieving further refinement of grain structure and improvement of mechanical properties in Al-12Si-4Cu-2Ni-1Mg alloy by Al-Ti-C-B master alloy addition and deep cryogenic treatment

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China Foundry 2025年第1期22卷 75-82页

作者： Lin-fei Xia Wen-bo Li zuo-shan Wei Yu-ying Wu Xiang-fa Liu China Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials Ministry of EducationShandong UniversityJinan 250061China Shandong Key Laboratory of Advanced Aluminum Materials and Technology Binzhou Institute of TechnologyBinzhou 256600ShandongChina

Near-eutectic Al-Si alloys are widely used in automotive manufacturing due to their superior wear resistance and high temperature *** of high Si content,the grain refinement of near-eutectic Al-Si alloy has been a problem for many *** this study,the effect of deep cryogenic treatment(DCT)on the microstructure and mechanical properties of Al-12Si-4Cu-2Ni-Mg alloy with addition of Al-Ti-C-B master alloy was fully *** show that the average grain size of the alloy is greatly reduced from 0.92 mm to 0.50 mm,and the eutectic Si and Al7Cu4Ni precipitates are spheroidized and refined in Al-12Si-4Cu-2Ni-Mg after DCT for 24 h and aging *** these changes of microstructures result in a significant increment of about 22.5%in elongation and a slight enhancement of about 6.8%in tensile ***,the refinement of microstructure also significantly improves the fatigue life of the alloy.

关键词： deep cryogenic treatment near-eutectic Al-Si master alloy microstructure mechanical properties

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Vibration safety assessment and parameter analysis of buried oil pipelines based on vibration isolation holes under strong surface impact

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Earthquake Engineering and Engineering Vibration 2025年第1期24卷 69-82页

作者： Wang Guobo Mei Hua Wang Jianning He Wei Yin Yao Zhai Yuxin zuo Pengfei College of Civil Engineering and Architecture Wenzhou UniversityWenzhou 325035China Zhejiang Engineering Research Center of Disaster Prevention and Mitigation for Coastal Soft Soil Foundation WenZhou 325035China China National Machinery Industry Co. Ltd.Beijing 100080China Faculty of Engineering China University of GeosciencesWuhan 430074China China Railway Construction Group Co. Ltd.Beijing 100072China School of Civil Engineering and Architecture Wuhan University of TechnologyWuhan 430070China

Strong surface impact will produce strong vibration,which will pose a threat to the safety of nearby buried pipelines and other important lifeline *** on the verified numerical method,a comprehensive numerical parameter analysis is conducted on the key influencing factors of the vibration isolation hole(VIH),which include hole diameter,hole net spacing,hole depth,hole number,hole arrangement,and soil *** results indicate that a smaller ratio of net spacing to hole diameter,the deeper the hole,the multi-row hole,the hole adoption of staggered arrangements,and better site soil conditions can enhance the efficiency of the VIH *** average maximum vibration reduction efficiency within the vibration isolation area can reach 42.2%.The vibration safety of adjacent oil pipelines during a dynamic compaction projection was evaluated according to existing standards,and the measurement of the VIH was recommended to reduce excessive *** single-row vibration isolation scheme and three-row staggered arrangement with the same hole parameters are suggested according to different *** research findings can serve as a reference for the vibration safety analysis,assessment,and control of adjacent underground facilities under the influence of strong surface impact loads.

关键词： vibration isolation hole buried oil pipeline strong surface impact vibration velocity vibration safety assessment

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