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Exercise promotes osteogenic differentiation by activating the long non-coding RNA H19/microRNA-149 axis

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World Journal of Orthopedics 2024年第4期15卷 363-378页

作者： Xu-Chang Zhou Dong-Xue Wang Chun-Yu Zhang Ya-Jing Yang Ruo-bing zhao Sheng-Yao Liu Guo-Xin Ni School of Sport Medicine and Rehabilitation Beijing Sport UniversityBeijing 100084China Department of Acupuncture and Moxibustion Hubei University of Chinese MedicineWuhan 430065Hubei ProvinceChina Department of Spinal Surgery The Second Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510260Guangdong ProvinceChina Department of Rehabilitation Medicine The First Affiliated Hospital of Xiamen UniversityXiamen 361003Fujian ProvinceChina

BACKGROUND Regular physical activity during childhood and adolescence is beneficial to bone development,as evidenced by the ability to increase bone density and peak bone mass by promoting bone formation.AIM To investigate the effects of exercise on bone formation in growing mice and to investigate the underlying mechanisms.METHODS 20 growing mice were randomly divided into two groups:Con group(control group,n=10)and Ex group(treadmill exercise group,n=10).Hematoxylin-eosin staining,immunohistochemistry,and micro-CT scanning were used to assess the bone formation-related indexes of the mouse femur.Bioinformatics analysis was used to find potential miRNAs targets of long non-coding RNA H19(lncRNA H19).RT-qPCR and Western Blot were used to confirm potential miRNA target genes of lncRNA H19 and the role of lncRNA H19 in promoting osteogenic differentiation.RESULTS Compared with the Con group,the expression of bone morphogenetic protein 2 was also significantly increased.The micro-CT results showed that 8 wk moderate-intensity treadmill exercise significantly increased bone mineral density,bone volume fraction,and the number of trabeculae,and decreased trabecular segregation in the femur of mice.Inhibition of lncRNA H19 significantly upregulated the expression of miR-149 and suppressed the expression of markers of osteogenic differentiation.In addition,knockdown of lncRNA H19 significantly downregulated the expression of autophagy markers,which is consistent with the results of autophagy-related protein changes detected in mouse femurs by immunofluorescence.CONCLUSION Appropriate treadmill exercise can effectively stimulate bone formation and promote the increase of bone density and bone volume in growing mice,thus enhancing the peak bone mass of mice.The lncRNA H19/miR-149 axis plays an important regulatory role in osteogenic differentiation.

关键词： Exercise Osteogenic differentiation Bone formation Bone mesenchymal stem cells Autophagy

Diffraction deep neural network-based classification for vector vortex beams

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Chinese Physics B 2024年第3期33卷 387-392页

作者：彭怡翔陈兵王乐赵生妹 Institute of Signal Processing and Transmission Nanjing University of Posts and Telecommunications (NJUPT)Nanjing 210003China Key Laboratory of Broadband Wireless Communication and Sensor Network Technology Ministry of EducationNanjing 210003China National Laboratory of Solid State Microstructures Nanjing UniversityNanjing 210093China

The vector vortex beam(VVB)has attracted significant attention due to its intrinsic diversity of information and has found great applications in both classical and quantum communications.However,a VVB is unavoidably affected by atmospheric turbulence(AT)when it propagates through the free-space optical communication environment,which results in detection errors at the receiver.In this paper,we propose a VVB classification scheme to detect VVBs with continuously changing polarization states under AT,where a diffractive deep neural network(DDNN)is designed and trained to classify the intensity distribution of the input distorted VVBs,and the horizontal direction of polarization of the input distorted beam is adopted as the feature for the classification through the DDNN.The numerical simulations and experimental results demonstrate that the proposed scheme has high accuracy in classification tasks.The energy distribution percentage remains above 95%from weak to medium AT,and the classification accuracy can remain above 95%for various strengths of turbulence.It has a faster convergence and better accuracy than that based on a convolutional neural network.

关键词： vector vortex beam diffractive deep neural network classification atmospheric turbulence

Copper-Free Click Chemistry-Mediated Assembly of Single Quantum Dot Nanosensor for Accurately Monitoring Locus-Specific m^(6)A in Cancer Cells

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Chinese Journal of Chemistry 2024年第5期42卷 499-504页

作者： Yue-Ying Li Ning-Ning zhao Yi-Xuan Geng Qian Han Jian-Ge Qiu bing-Hua Jiang Zi-Yue Wang Chun-Yang Zhang Translational Medicine Center The First Affiliated Hospital of Zhengzhou UniversityThe Academy of Medical SciencesHenan Institute of Medical and Pharmaceutical Sciences BGI CollegeZhengzhou UniversityZhengzhouHenan450052 China College of Chemistry Chemical Engineering and Materials ScienceShandong Normal UniversityJinanShandong250014 China School of Chemistry and Chemical Engineering Southeast UniversityNanjingJiangsu211189 China

N6-methyladenosine(m^(6)A)plays an important role in embryogenesis,nuclear export,transcription splicing,and protein translation control.Herein,we demonstrate a copper-free click chemistry-mediated assembly of single quantum dot(QD)nanosensor for accurately monitoring locus-specific m^(6)A in cancer cells.The m^(6)A-sensitive endoribonuclease MazF can digest the unmethylated A-RNA,and the intact m^(6)A-RNA then hybridizes with DNA probes a and b to produce a sandwich hybrid,initiating the click chemistry to generate probe a–b ligation product via first tandem ligation detection reaction(LDR)cycle.Subsequently,DNA probes c and d can hybridize with the probe a–b ligation product to generate the probe c–d ligation product via second LDR cycle.Both LDR cycles can be repeated through denaturation and annealing reaction to generate abundant biotin-/fluorophore-modified probe c–d ligation products that can easily assemble on the QD surface to induce distinct fluorescence resonance energy transfer(FRET)between QD and Cy5.This assay can be homogenously performed without the involvement of copper catalyst,m^(6)A-specific antibody,radioactive labeling,ligase enzyme,enzymatic reverse transcription,and next-generation sequencing.Moreover,it can discriminate even 0.01% m^(6)A level in complex samples and accurately measure cellular m^(6)A-RNA expression,providing a promising avenue for clinical diagnostics and biomedical research.

关键词： RNA methylation Click chemistry Förster resonance energy transfer Biosensor Cancer

Dynamic foraging strategy adaptation to heterogeneous environments contributes to social aggregation in snub-nosed monkeys

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Zoological Research 2024年第1期45卷 39-54页

作者： Lan zhao Sheng-Nan Ji Xiao-bing Du Jia-Hui Liu Bo-Lun Zhang Pei-Hua Li Yi-Jun Yang Bao-Guo Li Yan-Qing Guo Xiao-Guang Qi College of Life Sciences Northwest UniversityXi’anShaanxi 710069China Chengdu Institute of Biology Chinese Academy of SciencesChengduSichuan 610041China State Key Laboratory of Environmental Criteria and Risk Assessment Chinese Research Academy of Environmental SciencesBeijing 100012China

The dynamics of animal social structures are heavily influenced by environmental patterns of competition and cooperation.In folivorous colobine primates,prevailing theories suggest that larger group sizes should be favored in rainforests with a year-round abundance of food,thereby reducing feeding competition.Yet,paradoxically,larger groups are frequently found in high-altitude or high-latitude montane ecosystems characterized by a seasonal scarcity of leaves.This contradiction is posited to arise from cooperative benefits in heterogeneous environments.To investigate this hypothesis,we carried out a six-year field study on two neighboring groups of golden snub-nosed monkey(Rhinopithecus roxellana),a species representing the northernmost distribution of colobine primates.Results showed that the groups adjusted their movement and habitat selection in response to fluctuating climates and spatiotemporal variability of resources,indicative of a dynamic foraging strategy.Notably,during the cold,resource-scarce conditions in winter,the large group occupied food-rich habitats but did not exhibit significantly longer daily travel distances than the smaller neighboring group.Subsequently,we compiled an eco-behavioral dataset of 52 colobine species to explore their evolutionary trajectories.Analysis of this dataset suggested that the increase in group size may have evolved via home range expansion in response to the cold and heterogeneous climates found at higher altitudes or latitudes.Hence,we developed a multi-benefits framework to interpret the formation of larger groups by integrating environmental heterogeneity.In cold and diverse environments,even smaller groups require larger home ranges to meet their dynamic survival needs.The spatiotemporal distribution of high-quality resources within these expanded home ranges facilitates more frequent interactions between groups,thereby encouraging social aggregation into larger groups.This process enhances the benefits of collaborative actions and reproductive opportunities,whi

关键词： Social evolution Folivore paradox MLS Rhinopithecus roxellana Multi-benefits framework

Characteristics of gut microbiota determine effects of specific probiotics strains in patients with functional constipation

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Chinese Medical Journal 2024年第1期137卷 120-122页

作者： Haohao Zhang Lijuan Sun Zhixin zhao Yao Zhou Yuyao Liu Nannan Zhang Junya Yan Shibo Wang Renlong Li Jing Zhang Xueying Wang Wenjiao Li Yan Pan Meixia Wang bing Luo Mengbin Li Zhihong Sun Yongxiang zhao Yongzhan Nie State Key Laboratory of Targeting Oncology National Center for International Research of Bio-targeting TheranosticsGuangxi Key Laboratory of Bio-targeting TheranosticsCollaborative Innovation Center for Targeting Tumor Diagnosis and TherapyGuangxi Talent Highland of Bio-targeting TheranosticsGuangxi Medical UniversityNanningGuangxi 530021China State Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers and National Clinical Research Center for Digestive Diseases Xijing Hospital of Digestive DiseasesFourth Military Medical UniversityXi’anShaanxi 710032China Key Laboratory of Resource Biology and Biotechnology in Western China Ministry of EducationSchool of MedicineNorthwest UniversityXi’anShaanxi 710000China Key Laboratory of Dairy Biotechnology and Engineering Ministry of EducationKey Laboratory of Dairy Products ProcessingMinistry of Agriculture and Rural AffairsInner Mongolia Key Laboratory of Dairy Biotechnology and EngineeringInner Mongolia Agricultural UniversityHohhotInner Mongolia 010018China

To the Editor:Probiotics are a promising treatment modality for functional constipation(FC);however,the factors affecting individual responses to probiotics remain unclear.Growing evidence has identified that there is a strong relationship among the gut microbiota and constipation and related gut-brain axis.[1]The gut microbiota may modulate the gut functions via gut metabolites or trigger the release of gut hormones,such as peptide YY,gastric inhibitory polypeptide,and 5-hydroxytryptamine.[2]In turn,gut hormones affect gut secretion,motility,and sensation through their receptors located on epithelial,enteric,and smooth muscle cells.[2]Based on these findings,exogenous probiotics have been used in patients with constipation;however,few have produced consistent results.[3]In this study,we investigated the efficacy and safety of several specific probiotics strains for constipation,and also examined the potential reasons for the individualized effects of probiotics,which may facilitate interventional decisionmaking for FC patients.

关键词： constipation patients sensation

Taming heat with tiny pressure

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The Innovation 2024年第2期5卷 70-78页

作者： Kun Zhang Zhe Zhang Hailong Pan Haoyu Wang Xueting zhao Ji Qi zhao Zhang Ruiqi Song Chenyang Yu Biaohong Huang Xujing Li Huaican Chen Wen Yin Changlong Tan Weijin Hu Michael Wüubbenhorst Jiangshui Luo Dehong Yu Zhidong Zhang bing Li Shenyang National Laboratory for Materials Science Institute of Metal ResearchChinese Academy of SciencesShenyang 110016China School of Materials Science and Engineering University of Science and Technology of ChinaShenyang 110016China Laboratory for Soft Matter and Biophysics Department of Physics and AstronomyKU Leuven3001 LeuvenBelgium Institute of High Energy Physics Chinese Academy of SciencesBeijing 100049China Spallation Neutron Source Science Center Dongguan 523803China College of Materials Science and Engineering Sichuan UniversityChengdu 610064China School of Materials Science and Chemical Engineering Harbin University of Science and TechnologyHarbin 150080China Australian Nuclear Science and Technology Organisation Lucas HeightsNSW 2234Australia

Heat is almost everywhere.Unlike electricity,which can be easily manipulated,the current ability to control heat is still highly limited owing to spontaneous thermal dissipation imposed by the second law of thermodynamics.Optical illumination and pressure have been used to switch endothermic/exothermic responses of materials via phase transitions;however,these strategies are less cost-effective and unscalable.Here,we spectroscopically demonstrate the glassy crystal state of 2-amino-2-methyl-1,3-propanediol(AMP)to realize an affordable,easily manageable approach for thermal energy recycling.The supercooled state of AMP is so sensitive to pressure that even several megapascals can induce crystallization to the ordered crystal,resulting in a substantial temperature increase of 48 K within 20 s.Furthermore,we demonstrate a proof-of-concept device capable of programable heating with an extremely high work-to-heat conversion effi-ciency of383.Such delicate and efficient tuning of heat may remarkably facilitate rational utilization of waste heat.

关键词： illumination thermal rational

Evaluation of patches for rotator cuff repair:A systematic review and meta-analysis based on animal studies

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Bioactive Materials 2022年第4期7卷 474-491页

作者： Jinwei Yang Yuhao Kang Wanlu zhao Jia Jiang Yanbiao Jiang bing zhao Mingyue Jiao Bo Yuan Jinzhong zhao Bin Ma Evidence-Based Medicine Center School of Basic Medical SciencesLanzhou UniversityLanzhou730000China Reproductive Medicine Center Gansu Provincial Maternity and Child-Care HospitalLanzhou730050China Department of Sports Medicine Shanghai Jiao Tong University Affiliated Sixth People’s HospitalShanghai200233China College of Biomedical Engineering Sichuan UniversityChengdu610064China National Engineering Research Center for Biomaterials Chengdu610064China Key Laboratory of Evidence Based Medicine and Knowledge Translation of Gansu Province Lanzhou730000China

Based on the published animal studies,we systematically evaluated the outcomes of various materials for rotator cuff repair in animal models and the potentials of their clinical translation.74 animal studies were finally included,of which naturally derived biomaterials were applied the most widely(50.0%),rats were the most commonly used animal model(47.0%),and autologous tissue demonstrated the best outcomes in all animal models.The biomechanical properties of naturally derived biomaterials(maximum failure load:WMD 18.68[95%CI 7.71-29.66];P=0.001,and stiffness:WMD 1.30[95%CI 0.01-2.60];P=0.048)was statistically significant in the rabbit model.The rabbit model showed better outcomes even though the injury was severer compared with the rat model.

关键词： Rotator cuff patch Animal model Systematic review Meta-analysis Safety and effectiveness

An approach of dynamic response analysis of nonlinear structures based on least square Volterra kernel function identification

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Transportation Safety and Environment 2023年第2期5卷 1-13页

作者： Zhenhao Zhang Jun Xiong Zhenpeng zhao Fuming Wang Yi Zeng bing zhao Lu Ke School of Civil Engineering Changsha University of Science and TechnologyChangsha 410114HunanChina Guangxi Key Laboratory of Disaster Prevention and Engineering Safety Guangxi UniversityNanning 530004GuangxiChina College of Civil engineering and Architecture Guangxi UniversityNanning 530004GuangxiChina

Analysis of the dynamic response of a complex nonlinear system is always a difficult problem.By using Volterra functional series to describe a nonlinear system,its response analysis can be similar to using Fourier/Laplace transform and linear transfer function method to analyse a linear system’s response.In this paper,a dynamic response analysis method for nonlinear systems based on Volterra series is developed.Firstly,the recursive formula of the least square method is established to solve the Volterra kernel function vector,and the corresponding MATLAB programme is compiled.Then,the Volterra kernel vector corresponding to the nonlinear response of a structure under seismic excitation is identified,and the accuracy and applicability of using the kernel vector to predict the response of a nonlinear structure are analysed.The results show that the Volterra kernel function identified by the derived recursive formula can accurately describe the nonlinear response characteristics of a structure under an excitation.For a general nonlinear system,the first three order Volterra kernel function can relatively accurately express its nonlinear response characteristics.In addition,the obtained Volterra kernel function can be used to accurately predict the nonlinear response of a structure under the similar type of dynamic load.

关键词： nonlinear system Volterra series kernel function dynamic response least square method

Mode-based Damping Torque Analysis in Power System Low-frequency Oscillations

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CSEE Journal of Power and Energy Systems 2023年第4期9卷 1337-1347页

作者： Jingtian Bi Huadong Sun Shiyun Xu Ruihua Song bing zhao Qiang Guo the State Key Laboratory of Power Grid Safety and Energy Conservation China Electric Power Research InstituteBeijing 100192China

The mode-based damping torque analysis(M-DTA)method for studying the effect of an external controller on power system low-frequency oscillations is proposed in this paper.First,based on the interconnection model between the system and the controller in the frequency domain,the oscillation loop corresponding to the electromechanical oscillation mode is built,and then the mode-based damping torque of the controller can be calculated.Then,the application of the M-DTA method in the power system is illustrated.The derivation shows that in the single-machine infinite-bus power system,the M-DTA method is completely equivalent to the classical damping torque analysis(C-DTA)method.In the multi-machine power system,the mode-based damping torque directily reflects the effect of the controller on the oscillation mode,overcoming the shortcomings of the C-DTA method in which there is no direct correspondence between the damping torque and the oscillation mode.By deriving the relationship with the residue index,the M-DTA method shows higher accuracy than the residue method in applications,such as controller parameter adjustment.Finally,two example power systems are presented to demonstrate the application of the proposed M-DTA method.Index Terms-Electromechanical oscillation mode,FACTS,interconnection model in the frequency domain,mode-based damping torque analysis(M-DTA),power system low-frequency oscillation,PSS,residue method.

关键词： Electromechanical oscillation mode FACTS interconnection model in the frequency domain mode-based damping torque analysis(M-DTA) power system low-frequency oscillation PSS residue method.