检索结果-南通市图书馆

Cost-effective low-coverage whole-genome sequencing assay for the risk stratification of gastric cancer

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastrointestinal Oncology 2022年第3期14卷 690-702页

作者： Li-Ping Ye Xin-Li Mao Xian-bin Zhou Yi Wang Shi-Wen Xu sai-Qin He Zi-Liang Qian Xiao-Gang Zhang Li-Juan Zhai Jin-Bang Peng bin-bin Gu Xiu-Xiu Jin Ya-Qi Song Shao-Wei Li Key Laboratory of Minimally Invasive Techniques&Rapid Rehabilitation of Digestive System Tumor of Zhejiang Province Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical UniversityLinhai 317000Zhejiang ProvinceChina Department of Gastroenterology Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical UniversityLinhai 317000Zhejiang ProvinceChina Institute of Digestive Disease Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical UniversityLinhai 317000Zhejiang ProvinceChina Taizhou Hospital of Zhejiang Province Wenzhou Medical UniversityLinhai 317000Zhejiang ProvinceChina Suzhou Hongyuan Biotech Inc. Suzhou 215000Zhejiang ProvinceChina Prophet Genomics Inc. CaliforniaCA 95101United States Catcher Bio Inc. Hangzhou 310000Zhejiang ProvinceChina Department of Medicine Catcher Bio Inc.Hangzhou 310000Zhejiang ProvinceChina Taizhou Hospital of Zhejiang Province Zhejiang University School of MedicineLinhai 317000Zhejiang ProvinceChina

BACKGROUND Gastric cancer(GC), a multifactorial disease, is caused by pathogens, such as Helicobacter pylori(H. pylori) and Epstein-Barr virus(EBV), and genetic components.AIM To investigate microbiomes and host genome instability by cost-effective,low-coverage wholegenome sequencing,as biomarkers for GC subtyping.METHODS Samples from 40 GC patients were collected from Taizhou Hospital,Zhejiang Province,affiliated with Wenzhou Medical University.DNA from the samples was subjected to low-coverage wholegenome sequencing with a median genome coverage of 1.86×(range:1.03×to 3.17×) by Illumina×10,followed by copy number analyses using a customized bioinformatics workflow ultrasensitive chromosomal aneuploidy detector.RESULTS Of the 40 GC samples,20 (50%) were found to be enriched with microbiomes.EBV DNA was detected in 5 GC patients (12.5%).H.pylori DNA was found in 15 (37.5%) patients.The other 20(50%) patients were found to have relatively higher genomic instability.Copy number amplifications of the oncogenes,ERBB2 and KRAS,were found in 9 (22.5%) and 7 (17.5%) of the GC samples,respectively.EBV enrichment was found to be associated with tumors in the gastric cardia and fundus.H.pylori enrichment was found to be associated with tumors in the pylorus and antrum.Tumors with elevated genomic instability showed no localization and could be observed in any location.Additionally,H.pylori-enriched GC was found to be associated with the Borrmann type Ⅱ/Ⅲ and gastritis history.EBV-enriched GC was not associated with gastritis.No statistically significant correlation was observed between genomic instability and gastritis.Furthermore,these three different molecular subtypes showed distinct survival outcomes (P=0.019).EBV-positive tumors had the best prognosis,whereas patients with high genomic instability (CIN+) showed the worst survival.Patients with H.pylori infection showed intermediate prognosis compared with the other two subtypes.CONCLUSION Thus,using low-coverage whole-genome sequencing,GC can be classified into three

关键词： Gastric cancer Whole-genome sequencing Helicobacter pylori infections Epstein-Barr virus infections Genetic components Precision medicine

分支定价方法求解带二维装箱约束的车辆路径问题

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

控制理论与应用 2023年第3期40卷 409-418页

作者：季彬周赛琦张政中南大学交通运输工程学院湖南长沙410075

面向家具、电器等货物的物流配送场景,研究带二维装箱约束的车辆路径问题(2L–CVRP),构建了2L–CVRP的混合整数线性规划模型.为求解大规模2L–CVRP,构建了该问题集合划分模型,提出基于分支定价的方法.针对分支节点的松弛模型,基于列生... 详细信息

面向家具、电器等货物的物流配送场景,研究带二维装箱约束的车辆路径问题(2L–CVRP),构建了2L–CVRP的混合整数线性规划模型.为求解大规模2L–CVRP,构建了该问题集合划分模型,提出基于分支定价的方法.针对分支节点的松弛模型,基于列生成策略将其分解为线性规划主问题、带资源和二维装箱约束的最短路径子问题,并提出基于ng-route松弛策略的标签算法和基于禁忌搜索的装箱算法有效求解复杂子问题.仿真结果表明,提出的方法可高效求解大规模2L–CVRP,其中ng-route松弛策略能有效提升算法求解效率,研究成果为装箱约束下大规模车辆路径问题的高效求解提供了有效途径.

关键词：车辆路径混合整数线性规划分支定价二维装箱问题

巨噬细胞极化及与肾小管上皮细胞互动在缺血再灌注所致急性肾损伤中的作用

在线全文

学校读者我要写书评

暂无评论

生理学报 2022年第1期74卷 28-38页

作者：王薇赛文莉杨斌南通-莱斯特联合肾脏病学研究所南通大学附属医院肾内科南通226001 南通大学附属医院临床医学研究中心南通226001 莱斯特大学生命科学院心血管科学系肾脏研究组莱斯特大学医院莱斯特LE19HZ英国

急性肾损伤(acute kidney injury,AKI)是以肾功能急剧衰退为主要表现的常见危重临床疾病。缺血再灌注(ischemiareperfusion,IR)是AKI的主要病因之一。由于缺乏早期诊断方法和针对病因的特异性治疗,AKI的死亡率一直居高不下。IR快速诱发... 详细信息

急性肾损伤(acute kidney injury,AKI)是以肾功能急剧衰退为主要表现的常见危重临床疾病。缺血再灌注(ischemiareperfusion,IR)是AKI的主要病因之一。由于缺乏早期诊断方法和针对病因的特异性治疗,AKI的死亡率一直居高不下。IR快速诱发固有免疫反应,激活补体和固有免疫细胞,释放大量损伤相关分子如高迁移率族蛋白B1(high mobility group box-1,HMGB1)、趋化因子及炎症介质如caspase-3,进而募集免疫炎症细胞如M1型巨噬细胞(MФ)至肾损伤微环境中,引起肾小管上皮细胞(tubular epithelial cells,TECs)坏死和凋亡。死亡细胞及相关炎症进一步激活适应性免疫系统,在加重组织损伤的同时,也启动了M2型MФ参与的炎症清除、组织再生及修复的程序。职业吞噬细胞MФ和半职业化吞噬细胞TECs协同吞噬周围的损伤细胞,包括凋亡的MФ及TECs,是调节损伤、修复或纤维化转归的关键固有免疫细胞。近年来研究发现促红细胞生成素(erythropoietin,EPO)不仅与同源二聚受体(EPOR)2结合诱导红细胞生成,还与异源二聚受体(EPOR/βcR),亦称固有修复受体(innate repair receptor,IRR),结合而发挥组织保护作用。备解素(properdin)是补体旁路激活过程中唯一的正性调节因子,能有效识别并结合早期凋亡T细胞,协助其通过非补体激活依赖机制被MФ清除。我们课题组的前期研究表明MФ、TECs、EPO及其受体和备解素等关联,参与IR损伤和修复,但其确切机制需要进一步研究。外泌体作为细胞间信号传递的重要载体,参与多种肾脏疾病的发生及发展,但其在IR诱导AKI的过程中如何影响MФ和TECs间互动尚未完全明确。本文根据已有关于MФ和TECs在肾IR所致AKI中作用的研究结果,进一步讨论MФ极化、与TECs互动以及EPO、备解素及外泌体在其中的作用。

关键词：急性肾损伤促红细胞生成素受体外泌体缺血再灌注巨噬细胞备解素肾小管上皮细胞

High-drug-loading capacity of redox-activated biodegradable nanoplatform for active targeted delivery of chemotherapeutic drugs

在线全文

学校读者我要写书评

暂无评论

Regenerative Biomaterials 2020年第4期7卷 359-369页

作者： Hai Zhang Jianqin Yan Heng Mei Shengsheng Cai sai Li Furong Cheng Jun Cao bin He School of Chemical Engineering Sichuan UniversityNo.24 South Section 1Yihuan RoadChengdu 610065China National Engineering Research Center for Biomaterials Sichuan UniversityNo.29 Wangjiang RoadChengdu 610064China

Challenges associated with low-drug-loading capacity,lack of active targeting of tumor cells and unspecific drug release of nanocarriers synchronously plague the success of cancer therapy.Herein,we constructed active-targeting,redox-activated polymeric micelles(HPGssML)selfassembled aptamer-decorated,amphiphilic biodegradable poly(benzyl malolactonate-co-e-caprolactone)copolymer with disulfide linkage and p-conjugated moieties.HPGssML with a homogenous spherical shape and nanosized diameter(-150 nm)formed a low critical micellar concentration(10^-3mg/mL),suggesting good stability of polymeric micelles.The anticancer drug,doxorubicin(DOX),can be efficiently loaded into the core of micelles with high-drug-loading content via strong π-π interaction,which was verified by a decrease in fluorescence intensity and redshift in UV adsorption of DOX in micelles.The redox sensitivity of polymeric micelles was confirmed by size change and in vitro drug release in a reducing environment.Confocal microscopy and flow cytometry assay demonstrated that conjugating aptamers could enhance specific uptake of HPGssML by cancer cells.An in vitro cytotoxicity study showed that the half-maximal inhibitory concentration(IC50)of DOX-loaded HPGssML was two times lower than that of the control group,demonstrating improved antitumor efficacy.Therefore,the multifunctional biodegradable polymeric micelles can be exploited as a desirable drug carrier for effective cancer treatment.

关键词： good stability,π-conjugated moieties active-targeting,redox-activated

Microstructure evolution of eutectic Nb–24Ti–15Si–4Cr–2Al–2Hf alloy processed by directional solidification

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Rare Metals 2017年第6期36卷 472-477页

作者： Zhen Li sai-Nan Yuan Li-Na Jia bin Kong Zhen Hong Hu Zhang School of Materials Science and Engineering Beihang Universtity Beijing 100191 China

In this work, the near-eutectic Nb-24Ti-15Si-4Cr-2Al-2Hf（at%） alloy was directionally solidified at 1900 ℃ with withdrawal rates of 6, 18, 36, 50 mm·min^-1 and then heat-treated at 1450 ℃ for 12 h. The microstructure evolution was investigated. The results show that the microstructure of the directionally solidified（DS） alloy is composed of Nbss＋Nb5Si3 eutectics within the whole withdrawal rate range, while the variation of rates makes a great difference on the solidification routes,the morphology and size of Nb_（ss）＋Nb_5Si_3 eutectic cells.With the increase in withdrawal rates, the petaloid Nbss＋Nb5Si3 eutectic cells transform into granular morphology. After the heat treatment, a mesh structure Nbssis formed gradually which isolates the Nb5Si3, and the phase boundaries become smoother in order to reduce the interfacial energy. Moreover, two kinds of Nb5Si3 exist in the heat-treated（HT） samples identified by crystal form and element composition, which are supposed as α-Nb5Si3 and γ-Nb5Si3, respectively. This study exhibits significant merits in guiding the optimization of Nb-Si-based alloys＇ mechanical properties.

关键词： Nb-Si-based alloy Directional solidification Heat treatment Microstructure evolution Intermetallic

Preoperative laser reduces silicone oil use in primary diabetic vitrectomy

在线全文

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2022年第4期15卷 591-597页

作者： Wen-bin Zheng Xiao-Hu Ding Kun-Bei Lai Ji-Zhu Li Yu-Qing Wu Yuan Ma Zi-Ye Chen Shi-Da Chen sai-Nan Xiao bing-Qian Liu Ying Lin Tao Li State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangdong Provincial Key Laboratory of Ophthalmology and Visual ScienceGuangdong Provincial Clinical Research Center for Ocular DiseasesGuangzhou 510060Guangdong ProvinceChina

AIM: To identify the predictive factors and laser photocoagulation associated with the use of silicone oil as endotamponade during primary diabetic vitrectomy. METHODS: The medical and surgical records of 690 patients(798 eyes) who underwent primary diabetic vitrectomy at a tertiary eye hospital in China from January 2018 to December 2018 were reviewed in this retrospective cohort study. The patients’ baseline characteristics and preoperative treatments were recorded. The binary Logistic regression model was used to evaluate the risk factors for the use of silicone oil as endotamponade agent during primary vitrectomy for proliferative diabetic retinopathy(PDR)-related complications.RESULTS: Among 690 patients with mean age of 52.1±10.5 y(range: 18-85 y), 299/690(43.3%) were female. The 31.6% of the eyes received preoperative laser treatment, and 72.4% of the eyes received preoperative anti-VEGF adjuvant therapy. Non-clearing vitreous haemorrhage(VH) alone or combined with retinal detachment was the main surgical indication(89.5%) for primary vitrectomy. Silicone oil was used as endotamponade in 313(39.2%) eyes. Lack of preoperative laser treatment [odds ratio(OR) 0.66, 95% confidence interval(CI): 0.48-0.92;P=0.015] and older age(OR 0.96, 95%CI: 0.95-0.98;P<0.001) were predictors of silicone oil tamponade during primary vitrectomy for PDR. CONCLUSION: The lack of preoperative laser treatment is a significant predictor of silicone oil tamponade during primary vitrectomy for PDR. However, the severity of PDR relevant to silicone oil use should be further evaluated.

关键词： proliferative diabetic retinopathy vitrectomy laser photocoagulation silicone oil tamponade predictive factors

Urinary Creatinine Concentrations and Its Explanatory Variables in General Chinese Population:Implications for Creatinine Limits and Creatinine Adjustment

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2022年第10期35卷 899-910页

作者： JI sai sai LYU Yue bin QU Ying Li HU Xiao Jian LU Yi Fu CAI Jun Fang SONG Shi Xun ZHANG Xu LIU Ying Chun YANG Yan Wei ZHANG Wen Li LI Ya Wei ZHANG Ming Yuan CHEN Chen LI Cheng Cheng LI Zheng GU Heng LIU Ling CAI Jia Yi QIU Tian FU Hui JI S.John ZHAO Feng ZHU Ying CAO Zhao Jin SHI Xiao Ming China CDC Key Laboratory of Environment and Population Health National Institute of Environmental HealthChinese Center for Disease Control and PreventionBeijing 100021China School of Public Health Jilin UniversityChangchun 130021JilinChina Vanke School of Public Health Tsinghua UniversityBeijing 100084China

Objective The study aimed to analyze the applicability of the World Health Organization’s exclusionary guidelines for Urinary creatinine(Ucr)in the general Chinese population,and to identify Ucr related factors.Methods We conduct a cross-sectional study using baseline data from 21,167 participants in the China National Human Biomonitoring Program.Mixed linear models and restricted cubic splines(RCS)were used to analyze the associations between explanatory variables and Ucr concentration.Results The geometric mean and median concentrations of Ucr in the general Chinese population were 0.90 g/L and 1.01 g/L,respectively.And 9.36%samples were outside 0.3-3.0 g/L,including 7.83%below the lower limit and 1.53%above the upper limit.Middle age,male,obesity,smoking,higher frequency of red meat consumption and chronic kidney disease were associated significantly with higher concentrations of Ucr.Results of the RCS showed Ucr was positively and linearly associated with body mass index,inversely and linearly associated with systolic blood pressure,diastolic blood pressure,triglycerides level,and glomerular filtration rate,and were non-linearly associated with triiodothyronine.Conclusion The age-and gender-specific cut-off values of Ucr that determine the validity of urine samples in the general Chinese population were recommended.To avoid introducing bias into epidemiologic associations,the potential predictors of Ucr observed in the current study should be considered when using Ucr to adjust for variations in urine dilution.

关键词： Biomonitoring Urine Hydration correction Creatinine

Dynamic Response Analysis of the Equivalent Water Depth Truncated Point of the Catenary Mooring Line

在线全文

学校读者我要写书评

暂无评论

China Ocean Engineering 2017年第1期31卷 37-47页

作者： ZHANG Huo-ming KONG Ling-bin GUAN Wei-bing HUANG sai-hua FANG Gui-sheng College of Metrology and Measurement Engineering China Jiliang UniversityHangzhou 310018China State Key Laboratory of Satellite Ocean Environment Dynamics(Second Institute of Oceanography SOA)Hangzhou 310012China Institute of Hydraulic & Environmental Engineering Zhejiang University of Water Resources and Electric PowerHangzhou 310018China Mechanical and Automotive Engineering College Zhejiang University of Water Resources and Electric PowerHangzhou 310018China

The real-time computer-controlled actuators are used to connect the truncated parts of moorings and risers in the active hybrid model testing system. This must be able to work in model-scale real time, based on feedback input from the floater motions. Thus, mooring line dynamics and damping effects are artificially simulated in real time, based on a computer-based model of the problem. In consideration of the nonlinear characteristics of the sea platform catenary mooring line, the equations of the mooring line motion are formulated by using the lumped-mass method and the dynamic response of several points on the mooring line is investigated by the time and frequency domain analysis method. The dynamic response of the representative point on the mooring line is analyzed under the condition of two different corresponding upper endpoint movements namely sine wave excitation and random wave excitation. The corresponding laws of the dynamic response between the equivalent water depth truncated points at different locations and the upper endpoint are obtained, which can provide technical support for further study of the active hybrid model test.

关键词： mooting line truncated point dynamic response lumped-mass method time domain simulation

Research Progress on Chinese Medicine Immunomodulatory Intervention for Chronic Primary Immune Thrombocytopenia: Targeting Cellular Immunity

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Integrative Medicine 2019年第7期25卷 483-489页

作者： LIU Wen-bin LI sai YU Xiao-ling DAI Tie-ying GAO Rui-lan Institute of Hematology Research The First Affiliated Hospital of Zhejiang Chinese Medical University

Chronic primary immune thrombocytopenia(CITP) is the most common acquired autoimmune disease that seriously threaten the physical and mental health of patients. Compared with Western medicine treatment, the intervention and treatment of Chinese medicine(CM) has shown certain therapeutic advantages. This paper reviewed the new pathogenesis progress on T cell immune abnormality in CITP, and CM studies on interferes effects of cellular immune regulation of CITP in recent years. Qi deficiency failing to control blood and internal obstruction of blood stasis are the two common types of CM syndromes in CITP patients, the corresponding treatments include invigorating Pi(Spleen), supplementing qi, activating blood, as well as tonifying qi and activating yang, regulating Gan(Liver) to invigorate Pi. The authors also mentioned the problems in the research field of CM for CTIP treatment, and put forward new ideas for the research in the future.

关键词： chronic immune thrombocytopenia cellular immunity Chinese medicine