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Functional characterization of sex pheromone receptors in Spodoptera frugiperda, S. exigua, and S. litura moths

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Insect Science 2023年第2期30卷 305-320页

作者： sai Zhang Fang Liu bin Yang Yang Liu Gui-Rong Wang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBejingChina College of Horticulture and Plant Protection Yangzhou UniversityYangzhouChina Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhenChina

Moths possess an extremely sensitive and diverse sex pheromone processing system,in which pheromone receptors(PRs)are essential to ensure communication between mating partners.Functional properties of some PRs are conserved among species,which is important for reproduction.However,functional differentiation has occurred in some homologous PR genes,which may drive species divergence.Here,using genome analysis,17 PR genes were identified from Spodoptera frugiperda,S.exigua,and S.litura,which belong to 6 homologous groups(odorant receptor[OR]6,11,13,16,56,and 62);of which 6 PR genes(OR6,OR11,OR13,OR16,OR56,and OR62)were identified in S.frugiperda and S.exigua,and 5 PR genes were identified in S.litura,excluding OR62.Using heterologous expression in Xenopus oocytes,we characterized the functions of PR orthologs including OR6,OR56,and OR62,which have not been clarified in previous studies.OR6 orthologs were specifically tuned to(Z,E)-9,12-tetradecadienyl acetate(Z9,E12-14:OAc),and OR62 orthologs were robustly tuned to Z7-12:OAc in S.frugiperda and S.exigua.The optimal ligand for OR56 was Z7-12:OAc in S.frugiperda,but responses were minimal in S.exigua and S.litura.In addition,SfruOR6 was male antennae-specific,whereas SfruOR56 and SfruOR62 were male antennae-biased.Our study further clarified the functional properties of PRs in 3 Spodoptera moth species,providing a comprehensive understanding of the mechanisms of intraspecific communication and interspecific isolation in Spodoptera.

关键词： pheromone pheromone receptor Spodoptera Xenopus oocytes

From 1D Nanofibers to 3D Nanofibrous Aerogels: A Marvellous Evolution of Electrospun SiO_(2) Nanofibers for Emerging Applications

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Nano-Micro Letters 2022年第12期14卷 40-78页

作者： Cheng Liu sai Wang Ni Wang Jianyong Yu Yi-Tao Liu bin Ding Innovation Center for Textile Science and Technology College of TextilesDonghua UniversityShanghai 201620People’s Republic of China

One-dimensional(1D)SiO_(2) nanofibers(SNFs),one of the most popular inorganic nanomaterials,have aroused widespread attention because of their excellent chemical stability,as well as unique optical and thermal characteristics.Electrospinning is a straightforward and versatile method to prepare 1D SNFs with programmable structures,manageable dimensions,and modifiable properties,which hold great potential in many cutting-edge applications including aerospace,nanodevice,and energy.In this review,substantial advances in the structural design,controllable synthesis,and multifunctional applications of electrospun SNFs are highlighted.We begin with a brief introduction to the fundamental principles,available raw materials,and typical apparatus of electrospun SNFs.We then discuss the strategies for preparing SNFs with diverse structures in detail,especially stressing the newly emerging three-dimensional SiO_(2) nanofibrous aerogels.We continue with focus on major breakthroughs about brittleness-to-flexibility transition of SNFs and the means to achieve their mechanical reinforcement.In addition,we showcase recent applications enabled by electrospun SNFs,with particular emphasis on physical protection,health care and water treatment.In the end,we summarize this review and provide some perspectives on the future development direction of electrospun SNFs.

关键词： SiO_(2)nanofibers Nanofibrous aerogels Structural design Mechanical reinforcement Multifunctional applications

Experimental and numerical investigation on failure characteristics and mechanism of coal with different water contents

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International Journal of Coal Science & Technology 2023年第4期10卷 325-342页

作者： Xuewei Liu Haixiao Chen bin Liu sai Wang Quansheng Liu Yong Luo Jin Luo State Key Laboratory of Geomechanics and Geotechnical Engineering Institute of Rock and Soil MechanicsChinese Academy of SciencesWuhan 430071China Faculty of Engineering China University of GeosciencesWuhan 430074China Ping’an Mining Engineering Technology Research Institute Co. Ltd.Huainan 232001China

Water injection, as a widely used technique to prevent coal burst, can restrain the fractured coal seam and released the energy storage. In this study, laboratory tests were frstly carried out on standard coal specimens with fve diferent water contents (i.e., 0%, 0.6%, 1.08%, 1.5%, 2.0%, and 2.3%). The failure mode, fragment size, and energy distribution characteristics of coal specimens were investigated. Experimental results show that strength, elastic strain energy, dissipated energy, brittleness index, as well as impact energy index decrease with increasing water content. Besides, the failure mode transitions gradually from splitting ejection to tensile-shear mixed failure mode as water content increases, and average fragment size shows positively related to water content. Moreover, scanning electron microscope tests results indicate that water in coal sample mainly causes the mineral softening and defects increase. Furthermore, a numerical model containing roadway excavation was established considering the water on coal burst prevention. Modelling results revealed that water injection can reduce degree of coal burst and ejection velocity of coal blocks, while it will raise up the depth of crack zone and surface displacement of roadway. Combined with laboratory tests and numerical results, the micro mechanism, energy mechanism, and engineering signifcance of water injection on coal burst prevention were fnally analyzed.

关键词： Water content Coal burst Failure mode Energy Numerical simulation

Effects of seepage pressure on the mechanical behaviors and microstructure of sandstone

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Journal of Rock Mechanics and Geotechnical Engineering 2024年第6期16卷 2033-2051页

作者： Xuewei Liu Juxiang Chen bin Liu sai Wang Quansheng Liu Jin Luo State Key Laboratory of Geomechanics and Geotechnical Engineering Institute of Rock and Soil MechanicsChinese Academy of SciencesWuhan430071China Faculty of Engineering China University of GeosciencesWuhan430074China Key Laboratory of Safety for Geotechnical and Structural Engineering of Hubei Province School of Civil EngineeringWuhan UniversityWuhan430072China

Surrounding rocks of underground engineering are subjected to long-term seepage pressure,which can deteriorate the mechanical properties and cause serious disasters.In order to understand the impact of seepage pressure on the mechanical property of sandstone,uniaxial compression tests,P-wave velocity measurements,and nuclear magnetic resonance(NMR)tests were conducted on saturated sandstone samples with varied seepage pressures(i.e.0 MPa,3 MPa,4 MPa,5 MPa,6 MPa,7 MPa).The results demonstrate that the mechanical parameters(uniaxial compressive strength,peak strain,elastic modulus,and brittleness index),total energy,elastic strain energy,as well as elastic strain energy ratio,decrease with increasing seepage pressure,while the dissipation energy and dissipation energy ratio increase.Moreover,as seepage pressure increases,the micro-pores gradually transform into meso-pores and macro-pores.This increases the cumulative porosity of sandstone and decreases P-wave velocity.The numerical results indicate that as seepage pressure rises,the number of tensile cracks increases progressively,the angle range of microcracks is basically from 50-120to 80-100,and as a result,the failure mode transforms to the tensile-shear mixed failure mode.Finally,the effects of seepage pressure on mechanical properties were discussed.The results show that decrease in the effective stress and cohesion under the action of seepage pressure could lead to deterioration of strength behaviors of sandstone.

关键词： Rock mechanics Mechanical property Seepage pressure Numerical simulation Microcracks

Recent Advances in Ultrafine Fibrous Materials for Effective Warmth Retention

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Advanced Fiber Materials 2023年第3期5卷 847-867页

作者： sai Wang Cheng Liu Fei Wang Xia Yin Jianyong Yu Shichao Zhang bin Ding Innovation Center for Textile Science and Technology College of TextilesDonghua UniversityShanghai 201620China

Extremely cold environment has led to a variety of serious public health issues and posed huge burden on the social econ-omy,which is an urgent challenge to the human worldwide.Featured with comfort,convenience,and cost-effectiveness,fibrous materials have been selected as heat insulation materials to protect the human body against the cold for centuries.The advanced ultrafine fibers,with remarkable softness,small average diameter and pore size,and high porosity,have found extensive attention,as promising candidate for application in reducing the heat loss.In this review,the heat transfer mechanisms for single fiber and fiber assembly are provided,and the typical categories of ultrafine fibrous materials for warmth retention,classified as fibrous membrane and fibrous sponge in terms of aggregate structures,are systematically summarized.In particular,this review comprehensively discusses the fabrication strategies,structure characteristics,and significant properties of various ultrafine fibrous materials.Finally,the current challenges and future development prospects of ultrafine fibrous materials for effective warmth retention are highlighted.

关键词： Ultrafine fibers Membranes Sponges Warmth retention

High-drug-loading capacity of redox-activated biodegradable nanoplatform for active targeted delivery of chemotherapeutic drugs

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Regenerative Biomaterials 2020年第4期7卷 359-369页

作者： Hai Zhang Jianqin Yan Heng Mei Shengsheng Cai sai Li Furong Cheng Jun Cao bin He School of Chemical Engineering Sichuan UniversityNo.24 South Section 1Yihuan RoadChengdu 610065China National Engineering Research Center for Biomaterials Sichuan UniversityNo.29 Wangjiang RoadChengdu 610064China

Challenges associated with low-drug-loading capacity,lack of active targeting of tumor cells and unspecific drug release of nanocarriers synchronously plague the success of cancer therapy.Herein,we constructed active-targeting,redox-activated polymeric micelles(HPGssML)selfassembled aptamer-decorated,amphiphilic biodegradable poly(benzyl malolactonate-co-e-caprolactone)copolymer with disulfide linkage and p-conjugated moieties.HPGssML with a homogenous spherical shape and nanosized diameter(-150 nm)formed a low critical micellar concentration(10^-3mg/mL),suggesting good stability of polymeric micelles.The anticancer drug,doxorubicin(DOX),can be efficiently loaded into the core of micelles with high-drug-loading content via strong π-π interaction,which was verified by a decrease in fluorescence intensity and redshift in UV adsorption of DOX in micelles.The redox sensitivity of polymeric micelles was confirmed by size change and in vitro drug release in a reducing environment.Confocal microscopy and flow cytometry assay demonstrated that conjugating aptamers could enhance specific uptake of HPGssML by cancer cells.An in vitro cytotoxicity study showed that the half-maximal inhibitory concentration(IC50)of DOX-loaded HPGssML was two times lower than that of the control group,demonstrating improved antitumor efficacy.Therefore,the multifunctional biodegradable polymeric micelles can be exploited as a desirable drug carrier for effective cancer treatment.

关键词： good stability,π-conjugated moieties active-targeting,redox-activated

Microstructure evolution of eutectic Nb–24Ti–15Si–4Cr–2Al–2Hf alloy processed by directional solidification

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Rare Metals 2017年第6期36卷 472-477页

作者： Zhen Li sai-Nan Yuan Li-Na Jia bin Kong Zhen Hong Hu Zhang School of Materials Science and Engineering Beihang Universtity Beijing 100191 China

In this work, the near-eutectic Nb-24Ti-15Si-4Cr-2Al-2Hf（at%） alloy was directionally solidified at 1900 ℃ with withdrawal rates of 6, 18, 36, 50 mm·min^-1 and then heat-treated at 1450 ℃ for 12 h. The microstructure evolution was investigated. The results show that the microstructure of the directionally solidified（DS） alloy is composed of Nbss＋Nb5Si3 eutectics within the whole withdrawal rate range, while the variation of rates makes a great difference on the solidification routes,the morphology and size of Nb_（ss）＋Nb_5Si_3 eutectic cells.With the increase in withdrawal rates, the petaloid Nbss＋Nb5Si3 eutectic cells transform into granular morphology. After the heat treatment, a mesh structure Nbssis formed gradually which isolates the Nb5Si3, and the phase boundaries become smoother in order to reduce the interfacial energy. Moreover, two kinds of Nb5Si3 exist in the heat-treated（HT） samples identified by crystal form and element composition, which are supposed as α-Nb5Si3 and γ-Nb5Si3, respectively. This study exhibits significant merits in guiding the optimization of Nb-Si-based alloys＇ mechanical properties.

关键词： Nb-Si-based alloy Directional solidification Heat treatment Microstructure evolution Intermetallic

Comparisons of cloud detection among four satellite sensors on severe haze days in eastern China

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Atmospheric and Oceanic Science Letters 2018年第1期11卷 86-93页

作者： TAN sai-Chun ZHANG Xiao WANG Hong CHEN bin SHI Guang-Yu SHI Chong State Key Laboratory of Numerical Modeling of Atmospheric Sciences and Geophysical Fluid Dynamics Institute of Atmospheric PhysicsChinese Academy of Sciences Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters Nanjing University of Information Science and Technology College of Earth Science University of Chinese Academy of Sciences State Key Laboratory of Severe Weather/Institute of Atmospheric Composition Chinese Academy of Meteorological Sciences China Meteorological Administration Key Laboratory of Cloud-Precipitation Physics and Severe Storms Institute of Atmospheric Physics Chinese Academy of Sciences

云在天气、气候和地球能量平衡中非常重要。雾霾地区不同卫星云探测精度如何的研究较少。本论文详细比较了中国东部地区2015–2016年冬季2次严重雾霾天气时,"A-Train"星群上四个遥感器的云探测。结果显示,在雾霾区域,MODIS/Aqua真彩色图... 详细信息

云在天气、气候和地球能量平衡中非常重要。雾霾地区不同卫星云探测精度如何的研究较少。本论文详细比较了中国东部地区2015–2016年冬季2次严重雾霾天气时,"A-Train"星群上四个遥感器的云探测。结果显示,在雾霾区域,MODIS/Aqua真彩色图和CALIOP/CALIPSO清楚地观测到气溶胶层时,AIRS/Aqua、CALIOP和CPR/CloudSat没有观测到云,而MODIS则观测到云顶高度接近地面的云,表明MODIS将气溶胶误判为云。更多雾霾天气个例结果显示,在雾霾区域,MODIS云量比AIRS云量高13%–49%,平均高36%.

关键词：云探测气溶胶雾霾 MODIS AIRS CloudSat CALIPSO

Dynamic Response Analysis of the Equivalent Water Depth Truncated Point of the Catenary Mooring Line

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China Ocean Engineering 2017年第1期31卷 37-47页

作者： ZHANG Huo-ming KONG Ling-bin GUAN Wei-bing HUANG sai-hua FANG Gui-sheng College of Metrology and Measurement Engineering China Jiliang UniversityHangzhou 310018China State Key Laboratory of Satellite Ocean Environment Dynamics(Second Institute of Oceanography SOA)Hangzhou 310012China Institute of Hydraulic & Environmental Engineering Zhejiang University of Water Resources and Electric PowerHangzhou 310018China Mechanical and Automotive Engineering College Zhejiang University of Water Resources and Electric PowerHangzhou 310018China

The real-time computer-controlled actuators are used to connect the truncated parts of moorings and risers in the active hybrid model testing system. This must be able to work in model-scale real time, based on feedback input from the floater motions. Thus, mooring line dynamics and damping effects are artificially simulated in real time, based on a computer-based model of the problem. In consideration of the nonlinear characteristics of the sea platform catenary mooring line, the equations of the mooring line motion are formulated by using the lumped-mass method and the dynamic response of several points on the mooring line is investigated by the time and frequency domain analysis method. The dynamic response of the representative point on the mooring line is analyzed under the condition of two different corresponding upper endpoint movements namely sine wave excitation and random wave excitation. The corresponding laws of the dynamic response between the equivalent water depth truncated points at different locations and the upper endpoint are obtained, which can provide technical support for further study of the active hybrid model test.

关键词： mooting line truncated point dynamic response lumped-mass method time domain simulation