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<i>In Pursuit</i>of Porcine Pluripotent Stem Cells for Autologous Cell Therapy

Stem Cell Discovery 2014年第4期4卷 107-124页

作者： Vinod Verma Ashish Mehta Sanjay pal Manoj kumar Birbal Singh Ashok kumar Sanjeev Gautam Centre of Biotechnology Nehru Science Complex University of Allahabad Allahabad India National Heart Research Institute Singapore National Heart Centre Singapore Department of Microbiology & Immunology National Institute of Nutrition Hyderabad India Indian Veterinary Research Institute Regional Station Palampur India Department of Zoology MLK Post Graduate College Balrampur India Department of Biotechnology Kurukshetra University Kurukshetra India

Treatments to repair the human heart following regenerative diseases remain a challenge for medical science. Unlike lower vertebrate species the human heart lacks a regenerative pathway meaning that research has to be focused on cell transplantation. Porcines (Sus scrofa) are excellent models for cardiovascular disease and pluripotent stem cells (PSCs) generated from porcines will provide important clinical insights for cardiac cell therapy. This could open a new avenue of research into degenerative conditions as porcine is a more effective human proxy to work with. However, bona fide PSCs are currently available onlyin rodents (mouse, rat) and primates (monkey, human). Attempts to derivepluripotent stem cells (PSCs) from porcine have been going on for more than two decades with slow progress. Despite the fact that the porcine stem cells are under increasing glare of publicity due to milestone achievements in this area of research. Advances in stem cell technology, especially the genetic engineering, innovative cell culturing and induced pluripotency to generate stem cells has dramatically revolutionized the basic and applied investigations and applications of porcine stem cells. This review attempts to summarize the major signaling pathways involved in maintenance of pluripotency and the state of the art conceptual and technical progress for generating bona fide porcine PSCs.

关键词： Porcine Model Signaling Pathways Stem Cells Induced Pluripotent Stem Cells Pluripotency Regenerative Medicine Cell Therapy Epigenetics

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General-motors diet: A quick fix for steatotic live liver donors

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iLIVER 2023年第3期2卷 151-155页

作者： Anish Gupta Abhideep Chaudhary Gaurav Sood Niteen kumar Imtiakum Jamir Aditya Shriya Vipin pal Singh Rekha Subramaniyam Hitesh Soni Milind Mandwar Department of Hepato-Pancreato-Biliary Surgery and Liver Transplantation BLK MAX Super Specialty HospitalNew DelhiIndia

Background and aims:GM(General-Motors)diet is a low-calorie 7-day diet plan using complex carbohydrates used for quick weight *** optimisation is known to decrease steatosis and improve outcomes in liver *** evaluated the efficacy and feasibility of using GM diet in steatotic live liver donors(LLD).Methods:LLDs who either had BMI>30 kg/m^(2),Computerised tomography liver attenuation index(CT-LAI)10%were started on GM diet for 1 *** of these LLDs and their recipients were retrieved retrospectively and ***,liver function tests(LFT),MR fat-frac-tion,CT-LAI,hospital-stay and complications were analysed in LLDs while early graft dysfunction(EGD)and complications were assessed in ***:A total of 51 LLDs with mean age of 34.6?9.5 years with baseline mean weight of 80.6±10.8 kg and BMI of 29.1±2.78 kg/m^(2) were started on GM *** a week of following the GM diet,there was an average weight loss of 3.46±2.1 kg,a decrease in fat-fraction by 3.8%±2.7,and an improvement in CT-LAI by 6.7?3.7 *** patients could successfully undergo donor hepatectomy with an average postoperative ICU stay of 2.86±0.8 days and hospital stay of 6.82?0.81 *** donor had steatosis>10% on intraoperative Tru-cut biopsy and their post-operative outcomes were similar to normal liver *** were intra-abdominal collections in 2 donors requiring drainage and one required re-exploration for intestinal *** was EGD in 8 recipients(15.6%)and mortality in 1(1.9%)***:GM diet is safe and effective regimen to reduce steatosis and increase healthy LLD pool without compromising donor or recipient safety.

关键词： Steatosis GM diet weight loss donor optimization LDLT donor hepatectomy donor outcomes

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Mechanical and Crystalline Behavior of Polymeric Nanocomposites in Presence of Natural Clay

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Open Journal of Applied Sciences 2012年第4期2卷 277-282页

作者： Parthajit pal Mrinal Kanti Kundu Swinderjeetsingh Kalra Chapal kumar Das Department of Chemistry Dayanand Anglo-Vedic (D. A.-V.) College Kanpur India Materials Science Centre Indian Institute of Technology Kharagpur Kharagpur India

Fabrication of nanocomposites from immiscible polymer blend system has been represented in this work. A new type of natural clay named Halloysite nanotubes (HNTs) are modified by Polyethyleneimine (PEI) and these PEI grafted HNTs are incorporated into the immiscible blend system during melt mixing process to prepare halloysite based nanocomposites. Fourier Transform Infrared Spectroscopy (FTIR) study confirms the formation of PEI grafted HNTs. The nanocomposites are characterized by SEM for morphological study and, the dispersion manners of nanoclays by Transmission Electron Microscopy (TEM). Storage modulus is studied by Dynamic mechanical thermal analysis (DMTA) instrument. The tensile measurement explored better tensile property of nanocomposites as compared to the virgin blend. XRD is performed to determine the crystalline behavior of the nanocomposites as well as for blend. The above investigations reveal that the HNTs act as reinforcing as well as nucleating agent in the blend system.

关键词： Nanocomposite Mechanical Crystallinity Halloysite Polyethyleneimine

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Use of Isoproturon Imprinted Polymer Membranes as a Selective Recognition Platform in a Resistance Based Electrochemical Sensor

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Open Journal of Applied Biosensor 2013年第1期2卷 20-28页

作者： Krishna pal Singh Ramesh kumar Prajapati Saurabh Ahlawat Sweeti Ahlawat Manjul Mungali Satish kumar Clay Product Environment Study Technology Division CSIR-Central Building Research Institute Roorkee India Membrane Biophysics & Nanobiosensor Research Laboratory CBSH G.B. Pant University of Agriculture & Technology Pantnagar India Membrane Biophysics & Nanobiosensor Research Laboratory CBSH G.B. Pant University of Agriculture & TechnologyPantnagar India

Methacrylic acid with isoproturon has been utilized to prepare molecularly imprinted polymeric (MIP) membrane on Whatman filter paper no. 5 for selective electrochemical estimation of isoproturon pesticide. MIP membrane was prepared by radical polymerization and characterized using Ultra Violet spectrophotometer, Fourier Transform Infra Red (FTIR) spectroscopy and Scanning Electron Microscopy (SEM). Contact angle study was used to determine the surface energy of the MIP membrane and electrical conductivity measurements were performed by applying a small-amplitude alternating voltage (20 mV) with frequencies varying from 20 Hz to 80 kHz generated by a low-frequency wave form generator. Different isoproturon concentrations in samples were tested and analyzed. Results indicate linear increase in membrane resistance with increasing isoproturon in 10-3 to 10-6 M range. The selectivity of the electrochemical sensor was confirmed by testing isoproturon in presence of the structurally related compounds monouran and diuran. Results reveal highly selective and sensitive sensor, which can be employed for regular estimation of isoproturon in fields.

关键词： Sensor Isoproturon Molecular Imprinting Monouran Diuran

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Secure Spread-Spectrum Watermarking for Telemedicine Applications

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Journal of Information Security 2011年第2期2卷 91-98页

作者： Basant kumar Harsh Vikram Singh Surya pal Singh Anand Mohan 不详

This paper presents a secure spread-spectrum watermarking algorithm for digital images in discrete wavelet transform (DWT) domain. The algorithm is applied for embedding watermarks like patient identification /source identification or doctors signature in binary image format into host digital radiological image for potential telemedicine applications. Performance of the algorithm is analysed by varying the gain factor, subband decomposition levels, size of watermark, wavelet filters and medical image modalities. Simulation results show that the proposed method achieves higher security and robustness against various attacks.

关键词： Watermarking Spread-Spectrum Discrete Wavelet Transform Telemedicine

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Conductivity Studies in Proton Irradiated AgI-Ag2O-V2O5-TeO2 Super-Ionic Glass System

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Materials Sciences and Applications 2010年第2期1卷 59-65页

作者： Poonam Sharma Dinesh kumar Kanchan Meenakshi Pant Karan pal Singh 不详

The electrical properties of proton ion beam irradiated glass samples are carried out by impedance spectroscopy in the frequency range from 10 Hz to 32 MHz. The ion beam of energy 3 MeV and fluence of of 1014 particles cm-2 was chosen for irradiation. The conductivity of the super ionic glass samples increases after irradiation and other electrical parameters like dielectric constant, dielectric loss and modulus of the proton irradiated glass samples as a function of glass composition and temperature are observed to change. The dielectric constant and the dielectric losses are increased after irradiation and the modulus parameters confirm the non-Debye nature for irradiated samples also.

关键词： Proton-Irradiated Glass DC Conductivity Silver-Ion Scaling Modulus

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Anti-bacterial Properties of Transition Metal Complexes of Copper Metal Ion:A Mini Review

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Journal of Metallic Material Research 2022年第2期5卷 27-38页

作者： Abhay Nanda Srivastva Nisha Saxena Netra pal Singh Jayant kumar Department of Chemistry Nitishwar MahavidyalayaB.R.A.Bihar UniversityMuzarrafpur842002India University Department of Chemistry B.R.A.Bihar UniversityMuzaffarpur842001India Department of Chemistry M.R.M.CollegeL.N.Mithila UniversityDarbhanga846004India Department of Chemistry D.D.U.Gorakhpur UniversityGorakhpur273009India Graduate Student Department of ZoologyNitishwar MahavidyalayaB.R.A.Bihar UniversityMuzarrafpur842002India

Bacterial infections are a major cause for impulsive deaths in human *** infections of the respiratory,gastrointestinal and central nervous system account for the majority of cases of sudden *** available drugs are getting ineffective by each passing day as the mutation is very fast in these pathogenic microbes resulting in drug *** growing resistance of bacteria necessitates the development of new and effective compounds of desired characteristics that could bar the rapid development of bacterial cell inside of the host *** with cellular resistance for clinical antibiotics,co-bacterial infections during microbial attacks(***,fungus,protozoans etc.)also demand for some novel antibacterial drugs having high efficacy and minimal side effects on human *** antibiotics should also be compatible with remedies ongoing for core microbial ***,in demand of search for effective antibacterial moieties,the scope of transition metal complexes as drug gives a good signal against the pathogenic bacteria by inhibiting their *** action of metal complexes on bacterial cell may be due to impremiablity,enzymatic interruptions,ribosomal interactions,disturbance in the path of protein synthesis,denaturing of genetic materials *** the *** in complexes may interrupt the lipophilisity through the bacterial cell *** of metal ions in organic moieties behaving as ligand delocalizeπ-electrons upon the entire chelate ring and this chelation results in overlapping of ligand orbital and partial sharing of(+)ve charge of metal ion with donor *** structural modifications in metal and organic lone pair donor species are the supposed reasons for their enhanced antimicrobial activities against pathogenic *** present review focuses on the impact of recently synthesized,well characterized mono and binuclear transition metal complexes of Cu ions that have the potential to be the drug of th

关键词： Ligands Coordination compounds Antimicrobial activity Drug resistance

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Experimental Investigation of the Effect of Working Parameters on Wire Offset in Wire Electrical Discharge Machining of Hadfield Manganese Steel

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Journal of Surface Engineered Materials and Advanced Technology 2013年第4期3卷 295-302页

作者： Ashok kumar Srivastava Surjya Kanta pal Probir Saha Karabi Das Department of Me- chanical Engineering IIT Patna Bihar Department of Mechanical Engineering IIT Kharagpur West Bengal India Department of Metallurgical and Materials Engineering IIT Kharagpur West Bengal India.

In this study, a series of tests have been conducted in order to investigate the machinability evaluation of austenitic Hadfield manganese steel in the Wire Electrical Discharge Machine (WEDM). Experimental investigations have been carried out to relate the effect of input machining parameters such as pulse on-time (Ton), pulse off-time (Toff), wire feed (WF), and average gap voltage (V) on the wire offset in WEDM. No analytical approach gives the exact amount of offset required in WEDM and hence experimental study has been undertaken. In this paper, a mathematical model has been developed to model the machinability evaluation through the response surface methodology (RSM) capable of predicting the response parameter as a function of Ton, Toff, WF and V. The samples are tested and their average prediction error has been calculated taking the average of all the individual prediction errors. The result shows that this mathematical model reflects the independent, quadratic and interactive effects of the various machining parameters on cutting speed in WEDM process.

关键词： Hadfield Manganese Steel WEDM Pulse Time Wire Offset Average Gap Voltage Response Surface Methodology

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Near index matching enables solid diffractive optical element fabrication via additive manufacturing

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Light(Science & Applications) 2023年第10期12卷 2187-2198页

作者： Reut Orange kedem Nadav Opatovski Dafei Xiao Boris Ferdman Onit Alalouf Sushanta kumar pal Ziyun Wang Henrik von der Emde Michael Weber Steffen J.Sahl Aleks Ponjavic Ady Arie Stefan WHell Yoav Shechtman Russell Berrie Nanotechnology Institute Technion—Israel Institute of TechnologyHaifaIsrael Lorry Lokey Interdisciplinary Center for Life Sciences and Engineering Technion—Israel Institute of TechnologyHaifaIsrael Department of Biomedical Engineering Technion—Israel Institute of TechnologyHaifaIsrael School of Electrical Engineering Fleischman Faculty of Engineering Tel Aviv UniversityTel Aviv 69978Israel School of Physics and Astronomy University of LeedsLeedsUK School of Food Science and Nutrition University of LeedsLeedsUK Department of NanoBiophotonics Max Planck Institute for Multidisciplinary SciencesGöttingenGermany Department of Optical Nanoscopy Max Planck Institute for Medical ResearchHeidelbergGermany

Diffractive optical elements(DOEs)have a wide range of applications in optics and photonics,thanks to their capability to perform complex wavefront shaping in a compact ***,widespread applicability of DOEs is still limited,because existing fabrication methods are cumbersome and ***,we present a simple and cost-effective fabrication approach for solid,high-performance *** method is based on conjugating two nearly refractive index-matched solidifiable transparent *** index matching allows for extreme scaling up of the elements in the axial dimension,which enables simple fabrication of a template using commercially available 3D printing at tens-of-micrometer *** demonstrated the approach by fabricating and using DOEs serving as microlens arrays,vortex plates,including for highly sensitive applications such as vector beam generation and super-resolution microscopy using MINSTED,and phase-masks for three-dimensional single-molecule localization *** the advantage of making DOEs widely accessible by drastically simplifying their production,the method also overcomes difficulties faced by existing methods in fabricating highly complex elements,such as high-order vortex plates,and spectrum-encoding phase masks for microscopy.

关键词： matching enable vortex

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Development of Non-Labeled QCM Biosensor for the Detection of <i>β</i>-Galactosidase: A Comparative Study of Gold and Polystyrene Nanoparticles

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Advances in Nanoparticles 2013年第2期2卷 182-190页

作者： Krishna pal Singh Manav kumar Choudhary Iva Chianella Prashant Singh Cranfield Biotechnology Centre Cranfield Health Cranfield University Bedfordshire England Department of Chemistry D.A.V. (PG) College Dehradun H.N.B. Garhwal University Srinagar India Membrane Biophysics & Nanobiosensor Research Laboratory C.B.S H G. B. Pant University of Agriculture& Technology Pantnagar India

The performance of gold and polystyrene nanoparticles was investigated using quartz crystal microbalance (QCM) as sensor platform;β-Galactosidase antibody with corresponding antigen was utilized for the immunoreaction. The development of the immunosensor included: 1) formation of self assembled monolayers on quartz crystals;2a) immobilization of p-aminothiophenol functionalized gold nanoparticles on carboxyl-terminated self assembled monolayer, or 2b) immobilization of polystyrene nanoparticles on crystals modified with p-aminothiophenol self assembled monolayer;3) attachment of monoclonal anti β-Gal on nanoparticles;and 4) detection of target analyte. The nanoparticles used were synthesized in house and characterized by transmission electron microscopy and infrared spectroscopy. The results revealed that antibodies were strongly attached to functionalized gold nanoparticles;the weaker immobilization of antibodies to polystyrene nanoparticles provoked their detachment during antigen detection. When cross reactivity of polystyrene nanoparticles was checked using a different antigen (Brucella), displacement of antibody was not recorded, demonstrating specificity of the reaction. To the best of our knowledge this is the first direct comparison between behaviors of biosensors developed with two commonly used nanoparticles. The results showed that both nanoparticles produced biosensors capable to detect β-Gal. Nevertheless biosensors developed using polystyrene nanoparticles are simpler, cheaper and more eco-friendly than those developed using gold nanoparticles.

关键词： QCM Biosensor β-Galactosidase Gold Nanoparticle Polystyrene Nanoparticle

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