检索结果-南通市图书馆

Picomolar antimalarial agent from a Chinese medicinal plant

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China Chemistry 2022年第1期65卷 82-86页

作者： Bin Zhou Flavia M.Zimbres Joshua H.Butler Cheng-Hui Xu Reagan S.haney Yan Wu Maria B.Cassera Jian-Min Yue State Key Laboratory of Drug Research Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China Department of Biochemistry and Molecular Biology Center for Tropical and Emerging Global Diseases(CTEGD)University of GeorgiaAthensGeorgia 30602United States

In our long-term efforts searching for antimalarial agents from Chinese medicinal plants,seven novel dimeric sesquiterpenoids(1–7)with significant antimalarial activity were isolated and characterized from the whole plants of Sarcandra glabra subsp.brachystachys by solid data acquired by diverse methods.Among them,compound 3 with an EC50 value of 4.3 pM against the chloroquine-resistant Plasmodium falciparum is the most potent antimalarial agent reported hitherto,about 1,000-fold stronger than artemisinin.This article further consolidates and refines our previously delineated structure-activity relationship for this antimalarial compound class.

关键词： medicinal plants dimeric sesquiterpenoids antimalarial agent structure-activity relationship

High Content Screening: Science, Techniques and Applications

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Stem cell therapy for the treatment of Leydig cell dysfunction in primary hypogonadism

2008年

作者： Steven A. haney

ISBN: (数字)9780470229859

ISBN: (纸本)9780470039991

The authoritative reference on High Content Screening (HCS) in biological and pharmaceutical research, this guide covers: the basics of HCS: examples of HCS used in biological applications and early drug discovery, emphasizing oncology and neuroscience; the use of HCS across the drug development pipeline; and data management, data analysis, and systems biology, with guidelines for using large datasets. With an accompanying CD-ROM, this is the premier reference on HCS for researchers, lab managers, and graduate students.

关键词：

来源：畅想之星图书光盘版（南... 评论

在线全文

畅想之星图书光盘版（南通市图书馆镜像）

学校读者我要写书评

暂无评论

World Journal of Stem Cells 2016年第10期8卷 306-315页

作者： Taylor C Peak Nora M haney William Wang Kenneth J DeLay Wayne J Hellstrom Department of Urology School of MedicineTulane University

The production of testosterone occurs within the Leydig cells of the testes. When production fails at this level from either congenital, acquired, or systemic disorders,the result is primary hypogonadism. While numerous testosterone formulations have been developed, none are yet fully capable of replicating the physiological patterns of testosterone secretion. Multiple stem cell therapies to restore androgenic function of the testes are under investigation. Leydig cells derived from bone marrow, adipose tissue, umbilical cord, and the testes have shown promise for future therapy for primary hypogonadism. In particular, the discovery and utilization of a group of progenitor stem cells within the testes, known as stem Leydig cells(SLCs), has led not only to a better understanding of testicular development, but of treatment as well. When combining this with an understanding of the mechanisms that lead to Leydig cell dysfunction, researchers and physicians will be able to develop stem cell therapies that target the specific step in the steroidogenic process that is deficient. The current preclinical studies highlight the complex nature of regenerating this steroidogenic process and the problems remain unresolved. In summary, there appears to be two current directions for stem cell therapy in male primary hypogonadism. The first method involves differentiating adult Leydig cells from stem cells of various origins from bone marrow, adipose, or embryonic sources. The second method involves isolating, identifying, and transplanting stem Leydig cells into testicular tissue. Theoretically, in-vivo re-activation of SLCs in men with primary hypogonadism due to age would be another alternative method to treat hypogonadism while eliminating the need for transplantation.

关键词： Stem cell therapy Leydig cells Primary hypogonadism Stem Leydig cells Testosterone Bone marrow-derived stem cells Adipose-derived mesenchymal stem cells

Not yet IDEAL?-evidence and learning curves of minimally invasive pancreaticoduodenectomy

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Hepatobiliary Surgery and Nutrition 2020年第6期9卷 812-814页

作者： Felix Nickel Caelán Max haney Beat-Peter Müller-Stich Thilo Hackert Department of General Visceraland Transplantation SurgeryHeidelberg UniversityHospital HeidelbergHeidelbergGermany

In response to“What’s the next step in evaluating laparoscopic pancreaticoduodenectomy?”(http://***/article/view/29682/25710)by Fernando Burdío,Luís Grand and Ignasi Poves.First and foremost,we would like to express our sincere condolences on the passing of Professor Ignasi Poves.We were deeply saddened to hear of this and wish his family,friends and colleagues strength in these difficult times of grieving.

关键词： IDEAL learning Grand

Estimating Potential Nitrogen Mineralisation Using the Solvita Soil Respiration System

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Open Journal of Soil Science 2015年第12期5卷 319-323页

作者： Richard L. haney Elizabeth B. haney Grassland Soil and Water Research Laboratory Agricultural Research Service United States Department of Agriculture (USDA-ARS) Temple TX USA Texas AgriLife Research & Extension Center Texas A & M University Temple TX USA

Nitrogen (N) mineralisation contributes considerably to crop growth in fertilized and unfertilized fields. It is useful to be able to assess potential N mineralisation to increase fertilizer application efficiency, prevent excessive N runoff, and improve environmental system models. The microbes present in soil mineralize N based on many factors, including soil temperature and moisture, tillage, and levels of organic C and N. The measurement of soil’s ability to mineralize N is considered a good indicator of soil quality. Many methods have been developed to estimate N mineralisation in the laboratory and field. The 7-day anaerobic N mineralisation method developed in the 1960’s is considered reliable and is often used to compare new N-mineralisation testing methods. This study examines the use of soil CO2 evolution as determined using the Solvita Soil Respiration System (Solvita) for estimating N mineralisation by comparing it directly to the anaerobic N mineralisation test. Measured CO2 using Solvita was strongly correlated with anaerobic N mineralisation (r2 = 0.82). Results indicate that the Solvita Soil Respiration System can be used to rapidly assess soil respiration and relative N mineralisation potential in any given soil and is considerably faster and easier to perform in a laboratory setting than the anaerobic N mineralisation test.

关键词： Anaerobic N Nitrogen Mineralisation Soil Respiration

Isolation of Picocyanobacteria (Order Synechococcales) and Occurrence of the Cyanotoxin Anatoxin-A in a Shallow Mesotrophic Pond

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Water Resource and Protection 2023年第6期15卷 299-314页

作者： Nancy J. Leland Kimberley Crocker Pearson Marty K. Burke Jeffrey T. Miller Alison Watts James F. haney Department of Biological Sciences University of New Hampshire Center for Freshwater Biology and Ecotoxicology Durham New Hampshire USA Lim-Tex North Andover Massachusetts USA Brewster Natural Resources Advisory Commission Brewster Massachusetts USA Brewster Ponds Coalition Brewster Massachusetts USA Department of Civil and Environmental Engineering University of New Hampshire College of Engineering and Physical Sciences Durham New Hampshire USA

We have verified the use of a serial filtration method to isolate picocyanobacteria for analysis. We used eDNA metabarcoding to confirm the picocyanobacteria as members of the Order Synechococcales, Genus Cyanobium, specifically Cyanobium 6307. Fluorometric analysis using accessory pigments phycocyanin and phycoerythrin described periods of excess biomass, where the net growth rate model confirmed these conditions. The total anatoxin-a concentrations in the picocyanobacterial sample ranged from 0.0074 - 6.41 μg·L-1 representing a 40-fold difference over the entire sampling season. Sampling frequency of every three days appeared to be an important factor in capturing these changes in anatoxin-a concentration. During a period of excess biomass, we were able to establish a linear correlation between cyanobacterial biomass and Anatoxin-a concentrations.

关键词： Picocyanobacteria Anatoxin-a eDNA Phycocyanin Phycoerythrin

Comparison of Wheat Yield Simulated Using Three N Cycling Options in the SWAT Model

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Open Journal of Soil Science 2018年第8期8卷 197-211页

作者： Elizabeth Brooke haney Richard Lee haney Michael James White Jeffrey George Arnold Texas AgriLife Research and Extension Center Texas A & M University Temple USA USDA-ARS Grassland Soil and Water Research Laboratory Temple USA

The Soil and Water Assessment Tool (SWAT) model has been successfully used to predict alterations in streamflow, evapotranspiration and soil water;however, it is not clear how effective or accurate SWAT is at predicting crop growth. Previous research suggests that while the hydrologic balance in each watershed is accurately simulated with SWAT, the SWAT model over or under predicts crop yield relative to fertilizer inputs. The SWAT model now has three alternative N simulation options: 1) SWAT model with an added flush of N (SWAT-flush);2) N routines derived from the CENTURY model (SWAT-C);and 3) a one-pool C and N model (SWAT-One). The objective of this study was to evaluate the performance of SWAT-flush, SWAT-C, and SWAT-One as they affect wheat yield prediction. Simulated yields were compared to wheat yields in a 28-year fertilizer/wheat yield study in Lahoma, OK. Simulated yields were correlated with actual 28-year mean yield;however, none of the available N cycling models predicted yearly yields. SWAT-C simulated average yields were closer than other N sub-models to average actual yield. Annually there was a stronger correlation between SWAT-flush and actual yields than the other submodels. However, none of the N-cycling routines were able to accurately predict annual variability in yield at any fertilizer rate. We found that SWAT-C or SWAT-flush are the most viable choices for accurately simulating long-term average wheat yields although annual variations in yield prediction should be taken into consideration. Further research is needed to determine the effectiveness of SWAT-C and SWAT-flush in determining average and annual yield in various farming regions and with numerous agronomic crops.

关键词： SWAT Model Yield Prediction

Removal of Lithium Citrate from H3A for Determination of Plant Available P

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Open Journal of Soil Science 2017年第11期7卷 301-314页

作者： Richard L. haney Elizabeth B. haney Douglas R. Smith Michael J. White USDA-ARS Grassland Soil and Water Research Laboratory Temple USA Texas A & M University Texas AgriLife Research and Extension Center Temple USA

The soil extractant, H3A, has undergone several iterations to extract calcium (Ca), iron (Fe), aluminum (Al), potassium (K), phosphorus (P), ammonium (NH4-N) and nitrate (NO3-N) under ambient soil conditions. Few soil extractants currently used by commercial and university soil testing laboratories can perform multi-nutrient extraction without over- or under-estimating at least one nutrient. Soil pH and plant root exudates have a strong influence on nutrient availability and H3A was developed to mimic soil conditions. Lithium citrate was previously used in the H3A formulation, but resulted in a cloudy supernatant in some samples, complicating laboratory analyses. In this study, we removed lithium citrate and compared the nutrients extracted from the modified (H3A-4) to the established (H3A-3) solutions. We found that the new extractant, H3A-4, produced a clear supernatant even in soils with low pH and high iron and aluminum concentrations. H3A-4 accurately predicts plant available nutrients and is a viable choice for commercial and laboratory settings due to its ease of use.

关键词： Phosphate Soil Extraction Lithium Citrate

Alternative Methods for Analysis of Cyanobacterial Populations in Drinking Water Supplies: Fluorometric and Toxicological Applications Using Phycocyanin

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Water Resource and Protection 2018年第8期10卷 740-761页

作者： Nancy J. Leland James F. haney Lim-Tex North Andover MA USA Department of Zoology University of New Hampshire Durham NH USA

The management of cyanobacteria and potential exposure to associated biotoxins requires the allocation of scarce resources across a range of freshwater resources within various jurisdictions. Cost effective and reliable methods for sample processing and analysis form the foundation of the protocol yielding reliable data from which to derive important decisions. In this study the utilization of new methods to collect, process and analyze samples enhanced our ability to evaluate cyanobacterial populations. Extraction of phycocyanin using the single freeze thaw method provided more accurate and precise measurements (CV 4.7% and 6.4%), offering a simple and cost-effective means to overcome the influence of morphological variability. In-vacuo concentration of samples prior to ELISA analysis provided a detection limit of 0.001 μg·L?1 MC. Fractionation of samples (?1) = ?0.279 + (1.368 ? Log PC (μg·L?1) while in an Aphanizomemon spp. dominant system Log MC (ng·L?1) = 0.385 + (0.449 ? Log PC (μg·L?1). These methods and sampling protocol could be used in other aquatic systems across a broader regional landscape to estimate the levels of microcystins.

关键词： Cyanobacteria Size Fractions Fluorometry Monitoring Phycocyanin Extraction