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贵阳医学院学报 1986年第1期 67-69页

作者：杨爱云赵蓉贵阳医学院内科学教研室

报告3例急性单核细胞性白血病,骨髓除单核系异常增生外,尚有巨核细胞增生,原始及幼稚型巨核细胞增多,末梢血与 BM 出现小型巨核细胞,2例血小板增多,但有功能缺陷。BM 多系增生异常,反应其病变可能在 BM 系干细胞的早期分化阶段。

关键词：单核细胞白血病巨核细胞增殖

Investigating the effects of Pentoxifylline on human breast cancer cells using raman spectroscopy

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Journal of Innovative Optical Health Sciences 2015年第2期8卷 26-36页

作者： Peeyush N.Goel S.P.Singh C.Murali Krishna r.P.Gude Gude Lab ACTRECTata Memorial Center KhargharNavi Mumbai 410210 India Chilakapati Lab ACTRECTata Memorial Center KhargharNavi Mumbai 410210 India

Breast cancer is one of the leading causes of cancer-related deaths in a global *** the present study,biochemical changes exerted upon Pentoxifylline(PTX)treatment had been ap-praised in human breast cancer cells using raman *** are no clinically approved methods to monitor such therapeutic responses *** spectral profiling is suggestive of changes in DNA,protein and lipid contents showing a linear relationship with drug ***,multivariate analysis using principal component based linear-discriminant-analysis(PC-LDA)was employed for dlassifying the control and the PTX treated *** findings support the feasibility of raman spectroscopy as an alternate/adjunct label-free,objective method for monitoring drug-induced modifications against breast cancer cells.

关键词： Pentoifylline MDA-MB-231 breast cancer cells raman spectroscopy spectra multivariate analysis

Pathological and biochemical alterations of astrocytes in ovariectomized rats injected with D-galactose: A potential contribution to Alzheimer's disease processes

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南京医科大学学报(自然科学版) 2008年第5期 574-574页

作者： Hua, X. D. Lei, M. Ding, H. Han, Q. Y. Hu, G. Xiao, M. Nanjing med Univ, Dept Anat Histol & Pharmacol, Jiangsu Prov Key Lab Neurodegenerat, Nanjing 210029, Peoples r China

Astrocytes are implicated in the pathological changes of Alzheimer's disease. Our previous studies have demonstratedthat estrogen deprivation and oxidative stress act synergistically to accelerate0 the progress of Alzheimer's disease. Long-term D-galac-tose injection combined with ovariectomy may serve as a rodent model for Alzheimer's disease. To address the potential contributionof astroglia to the Alzheimer's disease pathogenesis, we investigated pathological and biochemical alterations of astrocytes under thisanimal model. Ovadectomized rats injected with D-galactose for 2 weeks showed extensive localization of glial fibrillary acidic proteinimmunoreactive astrocytes and slightly elevated glutathione levels in the hippocampus without significant impairments in the watermaze test and deficits of the cholinergic analyses, compared to the saline-injected rats. Ovariectomized rats injected with D-galactosefor 6 weeks, however, exhibited degeneration of astrocytes and decreased glutathione levels in the hippocampus, accompanied withsevere dysfunction of behavioral test and deficiency of cholinergic terminals. Electron microscopy further confirmed the pathologicalchanges of astrocytes, especially in the aggregated area of synapse and brain microvessels. Consistent with degeneration of perivascu-lar astrocytic endfeet, analysis of the horseradish peroxidase demonstrated an impairment of the blood-brain barrier *** findings indicate that biochemical and pathological alterations of astrocytes may partially contribute to exacerbating neuronaldeficits in the course of Alzheimer's disease. restoring neuroprotective potential of astrocytes may be a useful therapeutic target forAlzheimer's disease and other neurodegenerative diseases.

关键词： Alzheimer’s disease astrocytes estrogen deprivation oxidative stress rats blood-brain-barrier senile plaques estrogen mice neuroprotection expression cortex acetylcholinesterase neurodegeneration innervation

Multimolecular complex of Par-4 and E2F1 binding to Smac promoter contributes to glutamate-induced apoptosis in human-bone mesenchymal stem cells

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南京医科大学学报(自然科学版) 2008年第11期 1493-1493页

作者： Lu,C.Chen,J.Q.Zhou,G.P.Wu,S.H.Guan,Y.F.Yuan,C.S.Nanjing med Univ,Dept Pediat,Affiliated Hosp 1,Nanjing 210029,Jiangsu,Peoples r China.

Neural cells undergo glutamate-induced apoptosis in ischaemic brain tissue,in which prostate apoptosis response-4 gene (Par-4)is ***-bone mesenehymal stem cells can be utilized as an effective therapy for ischemic brain *** this study,we found that glutamate could induce apoptosis in human-bone mesenehymal stem cells,accompanied by increased expression of Par-4 gene and Smac release from *** Par-4 expression attenuated the glutamate-induced *** Par-4 protein and E2F1 protein could bind to E2F1-binding BS3 site on Smac promoter and participated in the formation of a pro- teins-DNA ***,in the eomplex,E2F1,not Par-4,was found to be directly bound to the Smac promoter,suggesting that Par-4 exerted indireetly its transcriptional control on the Smac gene though interacting with *** of full-length Par-4 in human-bone mesenchymal cells resulted in increased activity of the Smac *** addition,the indirect transcripional regula- tion of Par-4 on Smac depended on its COOH terminus-mediated interaction between Par-4 and *** conclude that the forma- tion of proteins-DNA complex,containing Par-4 protein,E2F1 protein and the Smac promoter,contributes to the pro-apoptotic effect on glutamate-treated human-bone mesenchymal stem cells.

关键词： protein-kinase-c transcription smac/diablo mechanisms stroke target cancer apaf-1 bcl-2 arf

EpiMix:A novel method to estimate effective reproduction number

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Infectious Disease Modelling 2023年第3期8卷 704-716页

作者： Shihui Jin Borame Lee Dickens Jue Tao Lim Alex r.Cook Saw Swee Hock School of Public Health National University of Singapore and National University Health SystemSingapore Lee Kong Chian School of medicine Nanyang Technological UniversitySingapore Department of Statistics and Data Science National University of SingaporeSingapore

Transmission potential of a pathogen,often quantified by the time-varying reproduction number r t,provides the current pace of infection for a disease and indicates whether an emerging epidemic is under *** this study,we proposed a novel method,EpiMix,for r t estimation,wherein we incorporated the impacts of exogenous factors and random effects under a Bayesian regression *** Integrated Nested Laplace Approx-imation,EpiMix is able to efficiently generate reliable,deterministic r t *** the simulations and case studies performed,we further demonstrated the method's robust-ness in low-incidence scenarios,together with other merits,including its flexibility in selecting variables and tolerance of varying reporting *** these make EpiMix a potentially useful tool for real-time r t estimation provided that the serial interval distri-bution,time series of case counts and external influencing factors are available.

关键词： Epidemics INLA regression reproduction number SArS-CoV-2 Transmission dynamics

Pathological and biochemical alterations of astrocytes in ovariectomized rats injected with D-galactose:A potential contribution to Alzheimer's disease processes

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南京医科大学学报（自然科学版） 2008年第6期28卷 803-803页

作者： Hua, X. D. Lei, M. Ding, H. Han, Q. Y. Hu, G. Xiao, M. Nanjing med Univ,Dept Anat Histol & Pharmacol,Jiangsu Prov Key Lab Neurodegenerat,Nanjing 210029,Peoples r China. NanjingmedUniv DeptAnatHistol&PharmacolJiangsuProvKeyLabNeurodegeneratNanjing210029PeoplesRChina

Astrocytes are implicated in the pathological changes of Alzheimer's disease. Our previous studies have demonstrated that estrogen deprivation and oxidative stress act synergistically to accelerate the progress of Alzheimer's disease. Long-term D-galactose injection combined with ovariectomy may serve as a rodent model for Alzheimer's disease. To address the potential contribution of astroglia to the Alzheimer's disease pathogenesis, we investigated pathological and biochemical alterations of astrocytes under this animal model. Ovadectomized rats injected with D-galactose for 2 weeks showed extensive localization of glial fibrillary acidic protein immunoreactive astrocytes and slightly elevated glutathione levels in the hippocampus without significant impairments in the water maze test and deficits of the cholinergic analyses, compared to the saline-injected rats. Ovariectomized rats injected with D-galactose for 6 weeks, however, exhibited degeneration of astrocytes and decreased glutathione levels in the hippocampus, accompanied with severe dysfunction of behavioral test and deficiency of cholinergic terminals. Electron microscopy further confirmed the pathological changes of astrocytes, especially in the aggregated area of synapse and brain microvessels. Consistent with degeneration of perivascular astrocytic endfeet, analysis of the horseradish peroxidase demonstrated an impairment of the blood-brain barrier permeability. These findings indicate that biochemical and pathological alterations of astrocytes may partially contribute to exacerbating neuronal deficits in the course of Alzheimer's disease. restoring neuroprotective potential of astrocytes may be a useful therapeutic target for Alzheimer's disease and other neurodegenerative diseases.

关键词：老年痴呆生物化学技术星形胶质细胞半乳糖

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玉米密植程度研究

作物学报 1963年第4期 381-398页

作者：范福仁莫惠栋秦泰辰胡雪华苏北农学院

本文根据9个玉米密度试验的资料,论述了:(1)不同密度下玉米生物产量及籽粒产量的变化规律及其回归方程;(2)不同密度下玉米40个性状项目的变化规律及其回归方程;(3)生产水平及肥料数量对玉米密度的影响;(4)不同密度下玉米群体的小气候特... 详细信息

本文根据9个玉米密度试验的资料,论述了:(1)不同密度下玉米生物产量及籽粒产量的变化规律及其回归方程;(2)不同密度下玉米40个性状项目的变化规律及其回归方程;(3)生产水平及肥料数量对玉米密度的影响;(4)不同密度下玉米群体的小气候特点。供试密度从每亩1,000—8,000株。

关键词：种植密度方程栽植密度玉米籽粒产量程度

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鸭乙肝病毒表面抗原的检测和肝脏组织学观察

临床肝胆病杂志 1988年第1期 39-41页

作者：孙成刚叶维法白求恩医科大学肝病研究所

鸭乙型肝炎病毒(DHBV)及其所致肝病已被认为研究乙型肝炎和肝癌的发生机理的实验模型。但由于感染DHBV的鸭血清中病毒DNA和DNA-P随鸭龄增长而逐渐下降至测不到的水平,检测鸭血清中DHBV-DNA和DNA-P不能完全反映实际感染状态。关于鸭血中D... 详细信息

鸭乙型肝炎病毒(DHBV)及其所致肝病已被认为研究乙型肝炎和肝癌的发生机理的实验模型。但由于感染DHBV的鸭血清中病毒DNA和DNA-P随鸭龄增长而逐渐下降至测不到的水平,检测鸭血清中DHBV-DNA和DNA-P不能完全反映实际感染状态。关于鸭血中DHBV表面抗原(DHBsAg)的检测及共与肝病的关系,国内外皆未见报道。因此,本实验对这一方面进行了研究,并对鸭DHBV感染的流行病学作了一定的探讨。

关键词： DHBV 肝病 DNA 肝脏鸭血清

Iptakalim alleviates rotenone-induced degeneration of Dopaminergic neurons through inhibiting microglia-mediated neuroinflammation

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南京医科大学学报(自然科学版) 2008年第1期 50-50页

作者： Zhou,F. Wu,J. Y. Sun,X. L. Yao,H. H. Ding,J. H. Hu,G. Nanjing med Univ,Dept Anat & Histol & Pharmacol,Neuropharmacol Lab,Nanjing 210029,Peoples r China.

Inhibition of microglia-mediated neuroinflammation has been regarded as a prospective strategy for treating neurodegenerative disorders,such as Parkinson's disease(PD). In the present study,we demonstrated that systematic administration with iptakalim(IPT),an adenosine triphosphate(ATP)-sensitive potassium channel(K-ATP) opener,could alleviate rotenone-induced degeneration of dopaminergic neurons in rat substantia nigra along with the downregulation of microglial activation and mrNA levels of tumor necrosis factor-alpha(TNF-alpha) and cyclooxygenase-2(COX-2). In rat primary cultured microglia,pretreatment with IPT suppressed rotenone-induced microglial activation evidenced by inhibition of microglial amoeboid morphological alteration,declined expression of EDl(a marker for activated microglia),and decreased production of TNF-alpha and prostaglandin E2(PGE(2)). These inhibitory effects of IPT could be reversed by selective mitochondrial KATP(mitoKATP) channel blocker 5-hydroxydecanoate(5-HD). Furthermore,pretreatment with IPT prevented rotenone-induced mitochondrial membrane potential loss and p38/c-jun N-terminal kinase(JNK) mitogen-activated protein kinase(MAPK) activation in microglia,which might in turn regulate microglial activation and subsequent production of TNF-alpha and PGE(2). These data strongly suggest that the K-ATP opener IPT may be a novel and promising neuroprotective drug via inhibiting microgliamediated neuroinflammation.

关键词： iptakalim ATP-sensitive potassium channel microglia rotenone neuroinflammation Parkinson’s disease sensitive potassium channels k-atp channels parkinsons-disease signal-transduction substantia-nigra rat-brain cells activation neuroprotection inflammation

K-ATP channel openers facilitate glutamate uptake by GluTs in rat primary cultured Astrocytes

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南京医科大学学报(自然科学版) 2008年第5期 684-684页

作者： Sun, X. L. Zeng, X. N. Zhou, F. Dai, C. P. Ding, J. H. Hu, G. Nanjing med Univ,Dept Anat Histol &Pharmacol,Neuropharmacol Lab,Nanjing 210029,Jiangsu,Peoples r China

Increasing evidence, including from our laboratory, has revealed that opening of ATP sensitive potassium channels(K-ATP channels) plays the neuronal protective roles both in vivo and in vitro. Thus K-ATP channel openers (KCOs) have been pro-posed as potential neuroprotectants. Our previous studies demonstrated that K-ATP channels could regulate glutamate uptake activity in PC12 cells as well as in synaptosomes of rats. Since glutamate transporters(GluTs) of astrocytes play crucial roles in glutamate up-take and KATP channels are also expressed in astrocytes, the present study showed whether and how KATP channels regulated the function of GluTs in primary cultured astrocytes. The results showed that nonselective KCO pinacidil, selective mitochondrial KCO diazoxide, novel, and blood-brain barrier permeable KCO iptakalim could enhance glutamate uptake, except for the sarcolemmal KCO P1075. Moreover pinacidil, diazoxide, and iptakalim reversed the inhibition of glutamate uptake induced by 1-methyl-4-phenylpyridinium (MPP+). These potentiated effects were completely abolished by mitochondrial K-ATP blocker 5-hydroxydecanoate. Furthermore, either diazoxide or iptakalim could inhibit MPP+-induced elevation of reactive oxygen species(rOS) and phosphoryla-tion of protein kinases C(PKC). These findings are the first to demonstrate that activation of K-ATP channel, especially mitochondri-al K-ATP channel, improves the function of GluTs in astrocytes due to reducing rOS production and downregulating PKC phospho-rylation. Therefore, the present study not only reveals a novel pharmacological profile of KCOs as regulators of GluTs, but also pro-vides a new strategy for neuroprotection.

关键词： K-ATP channel opener glutamate uptake mitochondria iptakalim sensitive potassium channels dopaminergic mid-brain neurons excitatory amino-acids sulfonylurea receptor transporters expression iptakalim brain activation cardioprotection