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Dual role of FGF in proliferation and endoreplication of Drosophila tracheal adult progenitor cells

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Journal of Molecular Cell Biology 2020年第1期12卷 32-41页

作者： Cristina de Miguel josefa Cruz David Martin Xavier Franch-Marro Institute of Evolutionary Biology(Consejo Superior de Investigaciones Cientificas-Universitat Pompeu Fabra) Passeig Maritim de la Barceloneta 3708003 BarcelonaSpain

Adult progenitor cells activation is a key event in the formation of adult *** Drosophilat formation of abdominal adult trachea depends on the specific activation of tracheal adult progenitors(tracheoblasts)at the Tr4 and Tr5 spiracular *** of these tracheoblasts generates a pool of tracheal cells that migrate toward the posterior part of the trachea by the activation of the branchless/fibroblast growth factor(Bnl/FGF)signaling to form the abdominal adult ***,we show that,in addition to migration,Bnl/FGF signaling,mediated by the transcription factor Pointed,is also required for tracheoblast *** tracheoblast activation relies on the expression of the FGF ligand bnl in their nearby ***,we show that,in the absence of the transcription factor Cut(Ct),Bnl/FGF signaling induces endoreplication of tracheoblasts partially by promoting fizzy-related ***,our results suggest a dual role of Bnl/FGF signaling in tracheoblasts,inducing both proliferation and endoreplication,depending on the presence or absence of the transcription factor Ct,respectively.

关键词： FGF trachea adult progenitors cut proliferation endoreplication Pnt Fzr

Current status and potential of morphometric sperm analysis

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Asian Journal of Andrology 2016年第6期18卷 863-870页

作者： Alejandro Maroto-Morales Olga Garcia-Alvarez Manuel Ramon Felipe Martinez-Pastor M Rocio Fernandez-Santos A josefa Soler Jose Julian Garde SaBio IREC (CSIC - UCLM -JCCM) Albacete Spain Biomedical Center Medical Faculty in Pilsen Charles University in Prague Pilsen Czech Republic Regional Center of Animal Selection and Reproduction (CERSYRA) JCCM Valdepenas Spain 4Institute for Animal Health and Cattle Development University of Leon LeOn Spain Department of Molecular Biology University of Leon Leon Spain Faculty of Pharmacy (UCLM) Albacete Spain

The spermatozoon is the most diverse cell type known and this diversity is considered to reflect differences in sperm function. How the diversity in sperm morphology arose during speciation and what role the different specializations play in sperm function, however, remain incompletely characterized. This work reviews the hypotheses proposed to explain sperm morphological evolution, with a focus on some aspects of sperm morphometric evaluation; the ability of morphometrics to predict sperm cryoresistance and male fertility is also discussed. For this, the evaluation of patterns of change of sperm head morphometry throughout a process, instead of the study of the morphometric characteristics of the sperm head at different stages, allows a better identification of the males with different sperm cryoconservation ability. These new approaches, together with more studies employing a greater number of individuals, are needed to obtain novel results concerning the role of sperm morphometry on sperm function. Future studies should aim at understanding the causes of sperm design diversity and the mechanisms that generate them, giving increased attention to other sperm structures besides the sperm head. The implementation of scientific and technological advances could benefit the simultaneous examination of sperm phenotype and sperm function, demonstrating that sperm morphometry could be a useful tool for sperm assessment.

关键词： computer-assisted sperm morphometric analysis mammals sperm function sperm morphometry

Low-cost experimental application of real-time kinematic positioning for increasing the benefits in cereal crops

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International Journal of Agricultural and Biological Engineering 2021年第3期14卷 194-199页

作者： Abdelhamid Tayebi josefa Gómez Marián Fernández Francisco Sáez de Adana Oscar Gutiérrez Computer Science Department University of AlcaláAlcaláde Henares 28801Spain

Traditional agriculture is facing several challenges worldwide such as increased population growth,rapid forestry and urbanization,resource scarcity,climate change,environmental pollution,competition among different ***,farmers need to improve productivity in order to maintain the output *** study attempted to evaluate the benefits of using Real-Time Kinematic(RTK)positioning in precision agriculture through a series of real measurements carried out when farming *** farming management actions involved in the cereal crop process(raise fallow,plow,sow,fertilize,mow,and harvest)have been done using an automatic guidance system that has reduced costs.A reduction of 20%has been quantified in the fuel,the amount of fertilizer,the labor costs and the hours of ***,the environmental impact has been also *** inexpensive system consisting of a reference base station near the field and a mobile unit mounted on the test vehicle has been installed in order to increase the benefits in cereal *** Navigation Satellite System(GNSS)systems including Global Positioning System(GPS),GLONASS,Galileo and Beidou have been used in the *** research serves as a practical guide to implementing a low-cost guidance system to achieve best management practice.

关键词： real-time kinematic low-cost positioning benefits guidance system cereal

Longitudinal Evaluation of Right Ventricle Function after Right Ventricle-Pulmonary Artery Shunt ***-Taussig Shunt

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Congenital Heart Disease 2021年第1期16卷 27-37页

作者： Nunzia Borrelli Jolanda Sabatino Martina Avesani josefa Paredes Manjit Josen Alain Fraisse Paolo Guccione Guido Michielon Giovanni Di Salvo Paediatric Cardiology Department Royal Brompton HospitalLondonSW36NPUK Paediatric Cardiology Department Bambino GesùPaediatric HospitalRome00165Italy Cardiology Department “Magna Graecia”UniversityCatanzaro88100Italy Paediatric Cardiology Department University of PaduaPadua35128Italy

Background:Still little is known about the impact on right ventricle function of the 2 main approaches to Norwood palliation in Hypoplastic left heart syndrome,the right ventricle-pulmonary artery shunt(RVPAS)and the modified Blalock-Taussig shunt(mBTS).Methods:The cohort included 27 patients with Hypoplastic left heart syndrome(10 in the mBTS group,17 in the RVPAS group).Longitudinal strain,tricuspid annulus peak systolic excursion and fractional area change were evaluated before Norwood and in four different breakpoints in the steady-state after Norwood procedure(30-days,90-days,140-days and 200-days after Norwood).Results:No significant differences were found in all standard echocardiographic functional parameters between the two groups at any ***,when we compared right ventricle function before and after Norwood procedure,longitudinal strain significantly improved in mBTS group compared to pre-surgical assessment(after 90-days:mBTS+27,35%±43,47 ***-8,20%±25,25,p=0,03;after 200-days:mBTS+10.19%±36.58 ***-21.64%±30.43,p=0.04).Conclusion:The mBTS group,which did not receive any ventriculotomy,showed a significant increase in right ventricle longitudinal strain during *** data support the use of longitudinal strain in Hypoplastic left heart syndrome evaluation and may suggest potential value in terms of cardiac mechanics in using mBTS,preserving the right ventricle integrity.

关键词： Hypoplastic left heart syndrome speckle tracking echocardiography congenital heart disease

Downregulation of the Basic Peroxidase Isoenzyme from Zinnia elegans by Gibberellic Acid

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Journal of Integrative Plant Biology 2010年第2期52卷 244-251页

作者： María josefa López Núez-Flores Jorge Gutirrez Laura V.Gómez-Ros Esther Novo Uzal Mariana Sottomayor Alfonso Ros Barceló Department of Plant Biology University of MurciaE-30100MurciaSpain Department of Plant Biology University of La CoruaE-15071La CorunaSpain IBMC University of Porto4150 PortoPortugal

Hypocotyl formation during the epigeal germination of seedlings is under strict hormonal regulation. In a 3 d old Zinnia elegans seedling system, gibberellic acid （GA3） exerts an opposite effect to that exerted by light on hypocotyl photomorphogenesis because GAz promotes an etiolated-like growth with an inhibition of radial （secondary） growth. For this reason, the effect of GA3 on the basic peroxidase isoenzyme from Z. elegans （ZePrx）, an enzyme involved in hypocotyl lignin biosynthesis, was studied. The results showed that GA3 reduces ZePrx activity, similarly to the way in which it reduces seedling secondary growth. This hormonal response is supported by the analysis of the ZePrx promoter, which contains four types of GA3-responsive ***： the W Box/O2S; the Pyr Box; the GARE; and the Amy Box. Taken together, these results suggest that ZePrx is directly regulated by GA3, with this effect matching the inhibitory effect of GA on the hypocotyl secondary growth.

关键词： GA Downregulation of the Basic Peroxidase Isoenzyme from Zinnia elegans by Gibberellic Acid cis Figure

Nutritional value of a partially defatted and a highly defatted black soldier fly larvae (Hermetia illucens L.) meal for broiler chickens: apparent nutrient digestibility,apparent metabolizable energy and apparent ileal amino acid digestibility

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Journal of Animal Science and Biotechnology 2017年第4期8卷 897-905页

作者： Achille Schiavone Michele De Marco Silvia Martínez Sihem Dabbou Manuela Renna josefa Madrid Fuensanta Hernandez Luca Rotolo Pierluca Costa Francesco Gai Laura Gasco Department of Veterinary Sciences University of Turin Largo Paolo Braccini 2 10095 Grugliasco TO Italy Department of Animal Production University of Murcia Campus de Espinardo 30071 Murcia Spain Department of Agricultural Forest and Food Sciences University of Turin Largo Paolo Braccini 2 10095 Grugliasco TO Italy Institute of Science of Food Production National Research Council Largo Paolo Braccini 2 10095 Grugliasco TO Italy

Background: The study aimed to determine the apparent total tract digestibility coefficients(ATTDC) of nutrients, the apparent metabolizable energy(AME and AMEn) and the amino acid(AA) apparent ileal digestibility coefficients(AIDC)of a partially defatted(BSFp) and a highly defatted(BSFh) black soldier fly larvae meal. The experimental diets were: a basal diet and two diets prepared by substituting 250 g/kg(w/w) of the basal diet with BSFp or BSFh, ***: Significant differences were found between BSFp and BSFh meals for ATTDC of the nutrients: BSFp resulted more digestible than BSFh, except for ATTDC of CP which did not differed between meals, while a statistical trend was observed for ATTDC of DM and EE. The AME and AMEn values were significantly(P < 0.05) different between the two BSF meals, with higher levels for BSFp(16.25 and 14.87 MJ/kg DM, respectively). The AIDC of the AA in BSFp ranged from 0.44 to 0.92, while in BSFh they ranged from 0.45 to 0.99. No significant differences were observed for the AA digestibility(0.77 and 0.80 for BSFp and BSFh, respectively), except for glutamic acid, proline and serine that were more digestible in the BSFh meal(P < 0.05).Conclusions: Defatted BSF meals can be considered as an excellent source of AME and digestible AA for broilers with a better efficient nutrient digestion. These considerations suggested the effective utilization of defatted BSF larvae meal in poultry feed formulation.

关键词： Amino acid Apparent digestibility Black soldier fly meal Broiler chicken Metabolizable energy

Minimizing sperm oxidative stress using nanotechnology for breeding programs in rams

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Journal of Animal Science and Biotechnology 2023年第6期14卷 2395-2410页

作者： Alejandro Jurado‑Campos Pedro Javier Soria‑Meneses Maria Arenas‑Moreira Carlos Alonso‑Moreno Virginia Rodriguez‑Robledo Ana josefa Soler Jose Julian Garde Maria del Rocio Fernandez‑Santos SaBio IREC(CSIC-UCLM-JCCM) Campus UniversitarioS/N02071 AlbaceteSpain. Departamento de Quimica Inorganica Organica Y Bioquimica‑Centro de Innovacion en Quimica Avanzada(ORFEO‑CINQA)Facultad de FarmaciaUniversidad de Castilla-La Mancha02008 AlbaceteSpain. Centro Regional de Investigacion Biomedicas Unidad nanoDrugUniversidad de Castilla-La Mancha02008 AlbaceteSpain. Facultad de Farmacia Universidad de Castilla La Mancha02071 AlbaceteSpain.

Background Artificial insemination(AI)is a routine breeding technology in animal ***,the temperature-sensitive nature and short fertile lifespan of ram sperm samples hamper its use in *** this sense,nanotechnology is an interesting tool to improve sperm protection due to the development of nanomaterials for AI,which could be used as delivery *** this work,we explored the feasibility of vitamin E nanoemulsion(NE)for improving sperm quality during *** With the aim of evaluating this proposal,ejaculates of 7 mature rams of Manchega breed were collected by artificial vagina and extended to 60×10^(6)spz/mL in *** containing control and NE(12 mmol/L)with and without exogenous oxidative stress(100μmol/L Fe2+/ascorbate)were stored at 22 and 15℃and motility(CASA),viability(YO-PRO/PI),acrosomal integrity(PNA-FITC/PI),mitochondrial membrane potential(Mitotracker Deep Red 633),lipoperoxidation(C11 BODIPY 581/591),intracellular reactive oxygen species(ROS)production and DNA status(SCSAR)monitored during 96 *** results show that NE could be used to maintain ram spermatozoa during transport at 15 and 22℃for up to 96 h,with no appreciable loss of kinematic and physiological characteristics of freshly collected *** The storage of ram spermatozoa in liquid form for 2-5 d with vitamin E nanoemulsions may lead more flexibility to breeders in AI *** view of the potential and high versatility of these nanodevices,further studies are being carried out to assess the proposed sperm preservation medium on fertility after artificial insemination.

关键词： Breeding technology Nanoemulsions Nanotechnology Sperm oxidative stress Vitamin E

Capsule endoscopy in patients refusing conventional endoscopy

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World Journal of Gastroenterology 2014年第23期20卷 7424-7433页

作者： Javier Romero-Vázquez Federico Argüelles-Arias josefa Maria García-Montes ángel Caunedo-álvarez Francisco Javier Pellicer-Bautista Juan Manuel Herrerías-Gutiérrez Gastroenterology Service Virgen Macarena University Hospital41071 SevilleSpain

Capsule endoscopy is nowadays the diagnostic technique of choice in the study of small bowel pathologies,allowing the non-invasive study of the entire *** has led,together with new technical advances,to the creation of two new models(PillCam ESO and PillCam Colon)for the study of esophageal and colonic *** two new capsules offer an interesting alternative to conventional endoscopy in the study of the upper and lower digestive tracts,because traditional endoscopy is often unpleasant and uncomfortable for the patient,can be painful,often requires moderate or deep sedation and is not without complications(hemorrhage,perforation,etc.).PillCam Colon is particularly important for its usefulness in the diagnosis of colonic polyps,and is a potentially useful tool in cases of incomplete colonoscopy or in colorectal cancer screening,even more when most patients are reluctant to undergo screening programs due to the said disadvantages of conventional *** article discusses the advantages of capsule endoscopy over conventional endoscopy,its current application possibilities and indications in routine clinical *** the various sections of the work,we assess the application of endoscopic capsule in different sections of the digestive tract(esophagus,stomach,and colon)and finally the potential role of panendoscopy with PillCam Colon.

关键词： Conventional endoscopy Capsule endoscopy Esophageal capsule endoscopy Colon capsule endoscopy Panendoscopy

Impairment of pre-mRNA splicing in liver disease: Mechanisms and consequences

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World Journal of Gastroenterology 2010年第25期16卷 3091-3102页

作者： Carmen Berasain Saioa Gońi josefa Castillo Maria Ujue Latasa Jesús Prieto Matias A Avila Division of Hepatologyand Gene Therapy CIMA University of Navarra Pamplona31008 Spain

Pre-mRNA splicing is an essential step in the process of gene expression in eukaryotes and consists of the removal ofintrons and the linking of exons to generate mature mRNAs. This is a highly regulated mechanism that allows the alternative usage of exons, the retention ofintronic sequences and the generation of exonic sequences of variable length. Most human genes undergo splicing events, and disruptions of this process have been associated with a variety of diseases, including cancer. Hepatocellular carcinoma (HCC) is a molecularly heterogeneous type of tumor that usually develops in a cirrhotic liver. Alterations in pre-mRNA splicing of some genes have been observed in liver cancer, and although still scarce, the available data suggest that splicing defects may have a role in hepatocarcinogenesis. Here we briefly review the general mechanisms that regulatepre-mRNA splicing, and discuss some examples that illustrate how this process is impaired in liver tumorigenesis, and may contribute to HCC development. We believe that a more thorough examination of pre-mRNA splicing is still needed to accurately draw the molecular portrait of liver cancer. This will surely contribute to a better understanding of the disease and to the development of new effective therapies.

关键词： Cell signalling Hepatocarcinogenesis PremRNA splicing Splicing factors Targeted anticancer therapy