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MicroRNA-149 Regulates Proliferation,Migration,and Invasion of Pituitary Adenoma Cells by Targeting ADAM12 and MMP14

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Current Medical Science 2022年第6期42卷 1131-1139页

作者： Zhuo ZHANG Agnes Schäfer benjamin Voellger jun-wen WANG Ting LEI Christopher Nimsky jörg W.Bartsch Department of Neurosurgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China Department of Neurosurgery Philipps University MarburgBaldingerstrasseMarburg35033Germany Sino-German Neuro-Oncology Molecular Laboratory Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China Center for Mind Brainand BehaviorHans-Meerwein-Strasse 6Marburg35032Germany

Objective Pituitary adenomas(PAs)can adapt an aggressive phenotype by invading adjacent brain structures with rapid cellular *** studies demonstrated that excessive expression of metalloproteases ADAM12 and MMP-14 is instrumental for the active proliferation and invasiveness of PA cells in vitro and of tumors in ***,the mechanisms regulating ADAM12 and MMP-14 expression in PAs remain *** Target gene prediction and transcriptomic profiling of invasive *** human PA samples were performed to identify miRNA species potentially involved in the regulation of ADAM12 and *** cellular analyses of miRNA functions,two mouse PA cell lines(AtT20 and TtT/GF)were transfected with miR-149-3p and miR-149-5p,*** effects of miR-149(3p and 5p)on expression levels of ADAM12 and MMP14 were determined by Western blotting followed by an analysis of proliferation and colony formation assays,scratch migration assays,and invasion *** A significant downregulation of miRNA-149 was observed in invasive *** PA(0.32 vs.0.09,P<0.0001).In AtT-20 and TtT/GF mouse PAs cells,transfection of mimic miRNA-149(3p and 5p)caused a significantly reduced cell proliferation and matrigel invasion,whilst the effect on cell migration was less *** strands of miRNA-149(3p and 5p)markedly reduced protein levels of ADAM12 and MMP-14 by at least 40%in both cell *** This study proved that the invasiveness of PA cells is,at least partly,regulated by miRNA-149-dependent expression of ADAM12 and MMP-14.

关键词： pituitary adenoma microRNA miRNA-149 ADAM12 MMP-14 invasion

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Prevalence and factors associated with vitamin C deficiency in inflammatory bowel disease

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World journal of Gastroenterology 2022年第33期28卷 4834-4845页

作者： benjamin Langan Gordon jonathan S Galati Stevie Yang Randy S Longman Dana Lukin Ellen j Scherl Robert Battat Department of Medicine New York Presbyterian/Weill Cornell Medical CenterNew YorkNY 10065United States Department of Gastroenterology and Hepatology New York Presbyterian/Weill Cornell Medical CenterNew YorkNY 10021United States

BACKGROUND Patients with inflammatory bowel disease(IBD) are prone to several nutritional deficiencies. However, data are lacking on vitamin C deficiency in Crohn’s disease(CD) and ulcerative colitis(UC) patients, as well as the impact of clinical, biomarker and endoscopic disease severity on the development of vitamin C *** To determine proportions and factors associated with vitamin C deficiency in CD and UC *** In this retrospective study, we obtained clinical, laboratory and endoscopic data from CD and UC patients presenting to the IBD clinic at a single tertiary care center from 2014 to 2019. All patients had an available plasma vitamin C level. Of 353 subjects who met initial search criteria using a cohort discovery tool, 301ultimately met criteria for inclusion in the study. The primary aim described vitamin C deficiency(≤ 11.4 μmol/L) rates in IBD. Secondary analyses compared proportions with deficiency between active and inactive IBD. Multivariate logistic regression analysis evaluated factors associated with *** Of 301 IBD patients, 21.6% had deficiency, including 24.4% of CD patients and 16.0% of UC patients. Patients with elevated C-reactive protein(CRP)(39.1% vs 16.9%, P jective inflammatory marker utilized for analysis(elevated CRP) was the only factor associated with deficiency(odds ratio = 3.1, 95%confidence interval: 1.5-6.6, P = 0.003). There was no difference in the presence of clinical symptoms of scurvy in those with vitamin C deficiency and those *** Vitamin C deficiency was common in IBD. Patients with elevated inflammatory markers and penetrating disease had higher rates of vitamin

关键词： Inflammatory bowel disease Crohn’s disease Ulcerative colitis Vitamin C deficiency Scurvy Malnutrition

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Peptide Variant Detection by a Living Yeast Biosensor via an Epitope-Selective Protease

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BioDesign Research 2023年第1期5卷 1-11页

作者： Tea Crnković benjamin j.Bokor Mead E.Lockwood Virginia W.Cornish Department of Chemistry Columbia UniversityNew YorkNY 10027USA. Department of Biological Sciences Columbia UniversityNew YorkNY 10027USA. School of General Studies Columbia UniversityNew YorkNY 10027USA. Department of Systems Biology Columbia UniversityNew YorkNY 10027USA. Department of Biological Engineering Massachusetts Institute of TechnologyCambridge02139 MAUSA.

We previously demonstrated that we could hijack the fungal pheromone signaling pathway to provide a living yeast biosensor where peptide biomarkers were recognized by G-protein-coupled receptors and engineered to transcribe a ***,we demonstrated that the protease could be reintroduced to the biosensor to provide a simple mechanism for distinguishing single-amino-acid changes in peptide ligands that,otherwise,would likely be difficult to detect using binding-based *** characterized the dose–response curves for five fungal pheromone G-protein-coupled receptors,peptides,and proteases—Saccharomyces cerevisiae,Candida albicans,Schizosaccharomyces pombe,Schizosaccharomyces octosporus,and Schizosaccharomyces *** scanning was carried out for the most selective of these—*** and ***—with and without the *** peptide variants were discovered,which showed diminished cleavage by the protease(CaPep2A and CaPep2A13A).Those peptides were then distinguished by utilizing the biosensor strains with and without the protease,which selectively cleaved and altered the apparent concentration of peptide required for half-maximal activation for 2 peptides—CaPep and CaPep13A,respectively—by more than one order of *** results support the hypothesis that the living yeast biosensor with a sequence-specific protease can translate single-amino-acid changes into more than one order of magnitude apparent shift in the concentration of peptide required for half-maximal *** further engineering by computational modeling and directed evolution,the biosensor could likely distinguish a wide variety of peptide sequences beyond the alanine scanning carried out *** the future,we envision incorporating proteases into our living yeast biosensor for use as a point of care diagnostic,a scalable communication language,and other applications.

关键词： apparent otherwise utilizing

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The emerging role of somatic tumor sequencing in the treatment of urothelial cancer

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Asian journal of Urology 2021年第4期8卷 391-399页

作者： Lexiaochuan Wen Cameron j.Britton Rohan Garje benjamin W.Darbro Vignesh T.Packiam Department of Urology Mayo ClinicRochesterMNUSA Division of Hematology OncologyBlood&Marrow TransplantationDepartment of Internal MedicineUniversity of IowaIowa CityIAUSA Stead Family Department of Pediatrics University of IowaIowa CityIAUSA Department of Urology University of IowaIowa CityIAUSA

The development of rapid genome sequencing has greatly enhanced our understanding of the molecular biology underlying many *** exome sequencing has highlighted the individualistic nature of malignancies on a patient-to-patient basis and begun to revolutionize therapeutic *** recent years,whole genome sequencing of urothelial malignancies has identified a host of somatic mutations which contribute to growth,progression,and metastasis of urothelial carcinoma of the bladder and upper tract urothelial *** genetic sequencing continues,additional targets will be identified,allowing development of novel therapeutic agents targeting cancer on a molecular level,with the goal of delivering highly individualized care based on the underlying mutational profile of the patient’s *** this review,we aim to discuss known genetic alterations of urothelial malignancy and the implications these mutations carry in terms of prognostication and development of targeted therapeutic *** will focus on RNA-expression profiling and genomic DNA profiling,with a focus on comprehensive whole exome and whole genome sequencing relative to selected urothelial carcinoma-associated genes and circulating tumor DNA analysis.

关键词： Tumor sequencing Whole exome sequencing Next-generation sequencing Somatic mutations Bladder cancer Urothelial carcinoma

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Minimally invasive local therapies for liver cancer

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Cancer Biology & Medicine 2014年第4期11卷 217-236页

作者： David Li josephine Kang benjamin j.Golas Vincent W.Yeung David C.Madoff Department of Radiology Division of Interventional Radiology New York-Presbyterian Hospital/Weill Cornell Medical Center Department of Medicine NYU Langone Medical Center Flushing Radiation Oncology Services Department of Surgery Division of Surgical Oncology New York-Presbyterian Hospital/Weill Cornell Medical Center New York

Primary and metastatic liver tumors are an increasing global health problem,with hepatocellular carcinoma(HCC)now being the third leading cause of cancer-related mortality *** treatment options for HCC remain limited,with Sorafenib as the only prospectively validated agent shown to increase overall *** resection and/or transplantation,locally ablative therapies and regional or locoregional therapies have filled the gap in liver tumor treatments,providing improved survival outcomes for both primary and metastatic *** invasive local therapies have an increasing role in the treatment of both primary and metastatic liver *** patients with low volume disease,these therapies have now been established into consensus practice *** review highlights technical aspects and outcomes of commonly utilized,minimally invasive local therapies including laparoscopic liver resection(LLR),radiofrequency ablation(RFA),microwave ablation(MWA),high-intensity focused ultrasound(HIFU),irreversible electroporation(IRE),and stereotactic body radiation therapy(SBRT).In addition,the role of combination treatment strategies utilizing these minimally invasive techniques is reviewed.

关键词： Liver hepatocellular carcinoma (HCC) metastasis

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Vascular calcification does not predict anastomotic leak or conduit necrosis following oesophagectomy

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World journal of Gastrointestinal Surgery 2019年第7期11卷 308-321页

作者： benjamin j jefferies Emily Evans james Bundred james Hodson john L Whiting Colm Forde Ewen A Griffiths College of Medical and Dental Sciences University of BirminghamBirmingham B15 2TTUnited Kingdom Department of Radiology University Hospitals Birmingham NHS Foundation TrustBirmingham B15 2WBUnited Kingdom Institute of Translational Medicine University Hospitals Birmingham NHS Foundation TrustBirmingham B15 2TTUnited Kingdom Department of Upper Gastrointestinal Surgery University Hospitals Birmingham NHS Foundation TrustBirmingham B15 2THUnited Kingdom Institute of Cancer and Genomic Sciences University of BirminghamBirmingham B15 2TTUnited Kingdom

BACKGROUND Anastomotic leaks(AL)and gastric conduit necrosis(CN)are serious complications following *** studies have suggested that vascular calcification may be associated with an increased AL rate,but this has not been validated in a United Kingdom *** To investigate whether vascular calcification identified on the pre-operative computed tomography(CT)scan is predictive of AL or *** Routine pre-operative CT scans of 414 patients who underwent oesophagectomy for malignancy with oesophagogastric anastomosis at the Queen Elizabeth Hospital Birmingham between 2006 and 2018 were retrospectively *** of the proximal aorta,distal aorta,coeliac trunk and branches of the coeliac trunk was scored by two *** relationship between these calcification scores and occurrence of AL and CN was then *** Esophagectomy Complications Consensus Group definition of AL and CN was *** Complication data were available in n=411 patients,of whom 16.7%developed either AL(15.8%)or CN(3.4%).Rates of AL were significantly higher in female patients,at 23.0%,compared to 13.9%in males(P=0.047).CN was significantly more common in females,(8.0%vs 2.2%,P=0.014),patients with diabetes(10.6%vs 2.5%,P=0.014),a history of smoking(10.3%vs 2.3%,P=0.008),and a higher American Society of Anaesthesiologists grade(P=0.024).Out of the 14 conduit necroses,only 4 occurred without a concomitant *** statistically significant association was found between calcification of any of the vessels studied and either of these *** analyses were then performed to identify whether a combination of the calcification scores could be identified that would be significantly predictive of any of the ***,the stepwise approach did not select any factors for inclusion in the final *** analysis was repeated for composite outcomes of those patients with either AL or CN(n=69,16.7%)and for those with both AL and CN(n=10,2.

关键词： Oesophagectomy Anastomotic leak Gastric conduit necrosis Calcification Computed tomography Ischaemia

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Fractional Modeling and Analysis of Coupled MR Damping System

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IEEE/CAA journal of Automatica Sinica 2016年第3期3卷 288-294页

作者： Bingsan Chen Chunyu Li benjamin Wilson Yijian Huang School of Mechanical and Automotive Engi-neering Fujian University of Technology Department of Chemical and Biological Engineering University of Wisconsin School of Mechanical and Automotive Engineering Fujian University of Technology

The coupled magnetorheological (MR) damping system addressed in this paper contains rubber spring and magnetorheological damper. The device inherits the damping merits of both the rubber spring and the magnetorheological damper. Here a fractional-order constitutive equation is introduced to study the viscoelasticity of the combined damper. An introduction to the definitions of fractional calculus, and the transfer function representation of a fractional-order system are given. The fractional-order system model of a magnetorheological vibration platform is set up using fractional calculus, and the function of displacement is presented. It is indicated that the fractional-order constitutive equation and the transfer function are feasible and effective means for investigating of magnetorheological vibration device. © 2014 Chinese Association of Automation.

关键词： Algebra Calculations Constitutive equations Damping Rubber Viscoelasticity

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Sound Modulation in Singing Katydids Using Ionic Polymer-Metal Composites （IPMCs）

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journal of Bionic Engineering 2013年第4期10卷 464-468页

作者： Yan Zhou Cheng-Wei Chiu Carlos j. Sanchez jorge M. Gonzalez benjamin Epstein David Rhodes S. Bradleigh Vinson Hong Liang Materials Science and Engineering Texas A&M University College Station TX 77843 USA Department of Mechanical Engineering Texas' A&M University College Station TX 77843 USA Department of Entomology Texas A&M University College Station TX77843 USA Department of Plant Science California State University Fresno CA 93740 USA OpCoast LLC Brick NJ08723 USA

Many insect families have evolved to produce and detect complex singing patterns for the purposes of mating, display of dominance, predator escape, and other needs. While the mechanisms of sound production by insects have been thoroughly studied, man-machine exploitation of such mechanisms has remained unreported. We therefore describe a method to modulate the frequency spectrum in the chirp call of a singing insect, Gampsocleis gratiosa （Orthoptera： Tettigoniidae）, a large katydid indigenous to China and commonly known as Guo Guo or Chinese Bush Cricket. The chirp modulation was achieved through the contact of a ribbon of lonic Polymer-Metal Composite （IPMC） against wing of the insect. The IPMC effectively served as an actuator when a small DC voltage was applied to the ribbon＇s faces. By applying a sequential on/off voltage waveform to the IPMC ribbon, the katydid＇s chirp was modulated in a corresponding manner. This configuration can be used as part of a broader application of using singing insects to harness their acoustic power to produce and propagate machine-induced messages into the acoustic environment.

关键词： chirp modulation katydid IPMC

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Utility of MtCOI polymerase chain reaction-restriction fragment length polymorphism in differentiating between Q and B whitefly Bemisia tabaci biotypes

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Insect Science 2009年第2期16卷 107-114页

作者： Wei-Hua Ma Xian-Chun Li Timothy j. Dennehy Chao-Liang Lei Mo Wang benjamin A. Degain Robert L. Nichols Department of Entomology and BI05 Institute University of Arizona Tuscon AZ 85721 USA Department of Plant Protection College of Plant Science and Technology Huazhong Agricultural University Wuhan China Cotton Incorporated 6399 Weston Parkway Cary NC 27513 USA

The invasive, insecticide-resistant, Q whitefly biotype, has gradually spread to other countries including the US via human-mediated movement of plant materials. We assessed the utility of the VspI-based mtCOI （mitochondrion cytochrome oxidase I） polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） technique as a rapid, cost-effective, and reliable alternative for differentiating the Q from the dominant B biotype in Arizona. Using the standard mtCOI gene sequencing and mtCOI PCR-RFLP techniques, we biotyped eight whitefly strains of five individuals each collected from poinsettia and cotton at different locations in Arizona. Complete concordance was observed between the two methods, with three strains being identified as the Q biotype and five samples as the B biotype. We also scanned the mtCOI gene sequences for VspI polymorphisms in the B and Q biotype whiteflies currently available in the GenBank database. This global screening revealed the existence of three and four VspI polymorphic types for the Q and B biotypes, respectively. Nevertheless, all three VspI polymorphic Q biotype whiteflies shared a common and unique VspI site that can be used to differentiate Q biotype from the four VspI polymorphic B biotype whiteflies identified. These results demonstrate that the VspI-based mtCOI gene PCR-RFLP provides a reliable diagnostic tool for differentiating the Q and B biotype whiteflies in the US and elsewhere.

关键词： Bemisia tabaci biotype diagnosis tool mtCOI gene PCR-RFLP Vspl enzyme

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Geographical distribution of GmTfl1 alleles in Chinese soybean varieties

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The Crop journal 2015年第5期3卷 371-378页

作者： Guifeng Liu Lin Zhao benjamin j.Averitt Ying Liu Bo Zhang Ruzhen Chang Yansong Ma Xiaoyan Luan Rongxia Guan Lijuan Qiu Soybean Research Institute Northeast Agricultural University Department of Crop and Soil Environmental Sciences VA Polytechnic Institute and State University The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI) Institute of Crop ScienceChinese Academy of Agricultural Sciences Heilongjiang Academy of Agricultural Sciences

Stem growth habit is an important agronomic trait in soybean and is subject to artificial selection. This study aimed to provide a theory for genotypic selection of stem growth habit for breeding purposes by analyzing the alleles of Gm Tfl1 gene in Chinese soybean varieties and establishing a database of Gm Tfl1 variation. Using knowledge of insertion and deletion(Indel) in the non-coding region and four single-nucleotide polymorphisms(SNPs) in the coding sequences of the Gm Tfl1 gene, four CAPS and one Indel markers were developed and used to test 1120 Chinese soybean varieties. We found that the dominant Gm Tfl1 allele was prevalent in accessions from the Northern ecoregion, whereas the recessive allele, Gmtfl1, was more common in the Southern ecoregion, and the proportions of Gm Tfl1 and recessive alleles were respectively 40.1% and 59.9% in the Huang-Huai ecoregion. The proportion of Gm Tfl1 decreased and that of Gmtfl1 increased, gradually from north to south. Allele Gm Tfl1-a was present in higher proportions in the Huang-Huai spring, Huang-Huai summer, and Northern spring sub-ecoregions than that in the other sub-ecoregions. Gm Tfl1-b was common in the Northeast spring, Northern spring and Southern summer sub-ecoregions. Gmtfl1-ta was found mainly in the Huang-Huai spring,Huang-Huai summer and Southern spring sub-ecoregions. The Gmtfl1-ab allele was distributed in all six soybean sub-ecoregions. The Gmtfl1-bb allele was distributed mainly in the Huang-Huai spring and summer and Southern spring and summer sub-ecoregions,but the Gmtfl1-tb allele was detected only in the Huang-Huai summer sub-ecoregion. The distributions of Gm Tfl1 and Gmtfl1 have shown no large changes in nearly 60 years of breeding, but the frequency of the recessive genotype Gmtfl1 has shown a rising trend in the last 20 years. This study provides a theoretical foundation for breeding new soybean varieties for different ecoregions.

关键词： Soybean Stem growth habit Gm Tfl1 Distribution

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