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Rapid evolution of regulatory element libraries for tunable transcriptional and translational control of gene expression

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Synthetic and Systems Biotechnology 2017年第4期2卷 295-301页

作者： Erqing Jin Lynn Wong Yun Jiao Jake Engel benjamin holdridge Peng Xu Department of Chemical Biochemical and Environmental EngineeringUniversity of Maryland Baltimore County1000 Hilltop CircleBaltimoreMD 21250United States Department of Food Science and Engineering Jinan University601 West Huangpu RoadGuangzhou 510632China

Engineering cell factories for producing biofuels and pharmaceuticals has spurred great interests to develop rapid and efficient synthetic biology tools customized for modular pathway *** the way,combinatorial gene expression control through modification of regulatory element offered tremendous opportunity for fine-tuning gene expression and generating digital-like genetic *** this report,we present an efficient evolutionary approach to build a range of regulatory control *** reported method allows for rapid construction of promoter,5'UTR,terminator and trans-activating RNA *** overlapping oligos with high portion of degenerate nucleotides flanking the regulatory element could be efficiently assembled to a vector expressing fluorescence *** approach combines high mutation rate of the synthetic DNA with the high assembly efficiency of Gibson *** constructed library demonstrates broad range of transcriptional or translational gene expression ***,both the promoter library and 50UTR library exhibits gene expression dynamics spanning across three order of *** terminator library and trans-activating RNA library displays relatively narrowed gene expression *** reported study provides a versatile toolbox for rapidly constructing a large family of prokaryotic regulatory *** libraries also facilitate the implementation of combinatorial pathway engineering principles and the engineering of more efficient microbial cell factory for various biomanufacturing applications.

关键词： Promoter 50UTR Terminator Riboregulatory Trans-activating RNA Library Synthetic biology

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Corrigendum to“Rapid evolution of regulatory element libraries for tunable transcriptional and translational control of gene expression”[Synth Syst Biotechnol 2(4)(2017)295–301]

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Synthetic and Systems Biotechnology 2019年第4期4卷 232-232页

作者： Erqing Jin Lynn Wong Yun Jiao Jake Engel benjamin holdridge Peng Xu Department of Chemical Biochemical and Environmental EngineeringUniversity of Maryland Baltimore County1000 Hilltop CircleBaltimoreMD21250USA Department of Food Science and Engineering Jinan University601 West Huangpu RoadGuangzhou510632China

The corresponding author apologizes for listing Lynn Wong and Yun Jiao as co-authors of this *** experimental data,including fluorescence,cell density(OD)for the T7 promoter,T7 terminator,ribosome binding sites(RBS)and trans-activating RNA libraries have been uploaded to Mendeley with the DOI number(https://***/10.17632/skmnwht6dk.1).

关键词： libraries DOI evolution

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