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Quantitation of Polydeoxyribonucletides (PDRNs) in Human Placental Extract by Fluorescence Spectroscopy Using Ethidium Bromide

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American Journal of Analytical Chemistry 2014年第12期5卷 784-795页

作者： Piyali Datta Chakraborty Sutapa Goswami Sudipta Bera Indranil mukhopadhyay Department of Human Genetics Indian Statistical Institute Kolkata India Department of Research and Development Albert David Ltd. Kolkata India

Characterization of an aqueous extract of human placenta, used as a licensed drug for wound healing, leads to the identification of several bioactive components including polydeoxyribonu-cleotides (PDRNs). PDRNs are mixture of DNA fragments of different molecular weight. A spectro-fluorimetric method of quantitation of PDRNs in the aqueous extract of human placenta by using ethidium bromide (EtBr) has been described here. It has been demonstrated by thin layer chromatography (TLC) followed by reversed phase HPLC that EtBr binds specifically with the PDRN fraction of the multi-component extract. The binding specificity of EtBr has been verified by the analysis of emission spectra of the extract. A concentration of 0.29 μg/ml EtBr exhibits a linear range of standard CT-DNA from 0.5 - 5 μg/ml of buffer (R2 = 0.992). The same concentration of EtBr shows a linear range of measurements of placenta extract from 5 - 35 μl/ml of buffer (R2 = 0.976). The points of the curve were the average of three sets where maximum variation observed was ±3%. PDRN content of the extract has been estimated based on the resultant fluorescence emission (after background correction) with respect to the standard calibration curve of calf thymus DNA (CT-DNA). Estimation of PDRN in a large number of batches of placenta extract (n = 100) has been done. The statistical analysis of the estimation was found to be significant and the lower and upper levels of PDRN were 158.30 and 239.03 μg/ml of the extract respectively. This easy-to-use method of estimation of PDRN in multi-component biological extract is reported for the first time. This will help in quantitation of PDRNs for other biological extracts.

关键词： Human Placental Extract Wound Healing PDRN Ethidium Bromide Fluorescence Spectroscopy

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Transfer learning of deep neural networks for predicting thermoacoustic instabilities in combustion systems

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能源与人工智能(英文) 2021年第3期5卷 339-350页

作者： Sudeepta Mondal Ashesh Chattopadhyay Achintya mukhopadhyay Asok Ray Department of Mechanical Engineering The Pennsylvania State UniversityUniversity ParkPA 16802USA Department of Mechanical Engineering Rice UniversityHoustonTX 77005USA Department of Mathematics The Pennsylvania State UniversityUniversity ParkPA 16802USA Department of Mechanical Engineering Jadavpur UniversityIndia

The intermittent nature of operation and unpredictable availability of renewable sources of energy(e.g.,wind and solar)would require the combustors in fossil-fuel power plants,sharing the same grid,to operate with large turn-down *** brings in new challenges of suppressing high-amplitude pressure oscillations(e.g.,ther-moacoustic instabilities(TAI))in *** pressure oscillations are usually self-sustained,as they occur within a feedback loop,and may induce severe thermomechanical stresses in structural components of combus-tors,which often lead to performance degradation and even system ***,prediction of thermoacoustic instabilities is a critical issue for both design and operation of combustion *** this perspective,it is important to identify operating conditions which can potentially lead to thermoacoustic *** this regard,data-driven approaches have shown considerable success in predicting the instability map as a function of operating ***,often the available data are limited to learn such a relationship efficiently in a data-driven approach for a practical combustion *** this work,a proof-of-concept demonstration of transfer learning is provided,whereby a deep neural network trained on relatively inexpensive experiments in an electrically heated Rijke tube has been adapted to predict the unstable operating conditions for a swirl-stabilized lean-premixed laboratory scaled combustor,for which data are expensive to *** operating spaces and underlying flow physics of these two combustion systems are different,and hence this work presents a strong case of using transfer learning as a potential data-driven solution for transferring knowledge across *** results show that the knowledge transfer from the electrically heated Rijke tube apparatus helps in formulating an accurate data-driven surrogate model for predicting the unstable operating conditions in the swirl-stabilized combustor,

关键词： Thermoacoustic instabilities Deep learning Transfer learning Rijke tube Lean-premixed combustion

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Integrated Technology Solutions for High Quality Strip Production

Integrated Technology Solutions for High Quality Strip Produ...

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第八届（2011）中国钢铁年会

作者： Ananya mukhopadhyay Andrea Polo Marco Ometto Danieli Automation Via Stringher433042 ButtrioUdineItaly

Quality of strip is mainly characterized by mechanical property, dimensional tolerance, and surface appearance. Uniform mechanical property, desired tolerance, and high surface finish are essential to produce high end products. In case of mechanical property, while much of this can be achieved through sophisticated process control and good operational practice, needless to say that a good quality slab is an imperative for this. Thus, cleanliness, and internal soundness of slabs are some of the prerequisites. Danieli’s philosophy of Quality Strip Production involves through-process control and optimization of casting and rolling processes. The automation solutions for casting process such as Liquid Pool Control System, Dynamic Secondary Cooling, and Dynamic Soft Reduction provide slab with internal soundness, which is free from internal defect, segregation and give favourable grain structure through improved solidification. On the other hand, rolling solution such as Coil Quality Estimation ensures desired mechanical property and its uniformity over entire length of a strip in real-time processing in the rolling mill. An intergrated solution, therefore, is the key. Furthermore, Danieli is planning to integrate automation solutions for bulging control and Inclusion Control to present the inclusion map of the slab. Thus, the integration and control of automation solutions for casting and rolling provide the key for future products of superior quality.

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Studies on Transport and Structural Properties of Si:H Network in the Amorphous-to-microcrystalline Transition Phase

Studies on Transport and Structural Properties of Si:H Netwo...

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第十五届国际光伏科学与工程大会

作者： Sumita mukhopadhyay Romyani Goswami Swati Ray Energy Research Unit Indian Association for the Cultivation ofScienceJadavpurKolkata 700 032.India

Transition from amorphous to microcrystalline phase in silicon film has been realised at high power-pressure varying the hydrogen dilution in RFPECVD system. At the transition region the dark conductivity of the film is (σ) ～2.67×10 Scm with its activation energy (E) of～480meV. IR vibrational spectra have shown low microstructural defects in the films compared to films deposited at low power-pressure. Bonded H-content changes from 15.6% to 5.6% in the transition region. The material deserves high promise to be used as intrinsic layers in solar cells.

关键词： RFPECVD Transition FTIR

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Studies on Processing and Characterization of Hydroxyapatite Biomaterials from Different Bio Wastes

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Journal of Minerals and Materials Characterization and Engineering 2012年第1期11卷 55-67页

作者： Sudip Mondal Biswanath Mondal Apurba Dey Sudit S. mukhopadhyay Centre for Advanced Material Processing Central Mechanical Engineering Research InstituteMahatma Gandhi Avenue Durgapur-713209 India Department of Biotechnology National Institute of Technology Mahatma Gandhi Avenue Durgapur-713209 India

Development of suitable materials that acts as an interface between the implant and tissues in body system structurally, mechanically and bio functionally is important for the success of tissue engineering. This motivated materials scientists and biologists to find out suitable bioactive materials for the aforementioned purpose. There has been growing interest in developing bioactive synthetic ceramics that could closely mimic natural apatite characteristics. Hydroxyapatite (HAp) has been widely used as a biocompatible ceramic but mainly for contact with bone tissue, due to its resemblance to mineral bone. This study presents the synthesis and characterization of HAp materials from different sources like bovine bone and fish scales and their application in tissue engineering. The phase purity and crystallinity of different calcined HAp powder was determined by XRD and FTIR analysis. The Thermo Gravimetric and Differential Thermal Analysis were carried out to show the thermal stability of the HAp powder. The morphology of the powder was observed under Scanning Electron Microscopy (SEM). Cytotoxicity evaluation of the developed powder was carried out in RAW macrophage like cell line media for an incubation period of 72 hours. These results proved the biocompatibility of HAp powders obtained from different biosources for tissue engineering applications.

关键词： Hydroxyapatite Biomaterials Tissue Engineering Cytotoxicity

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Inhibition of CB1 Receptor Protects Against Cisplatin-Induced Nephrotoxicity

Inhibition of CB1 Receptor Protects Against Cisplatin-Induce...

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第十五届全国泌尿外科学术会议

作者： Partha mukhopadhyay Mohanraj Rajesh Pal Pacher Laboratory of Physiological Studies NIH/NIAAA

Objectives: The endocannabinoid system was an emerging target of pharmacotherapy. We aimed to explore the effects of pharmacologic inhibition of CB1 receptor in vivo models of cisplatin-induced nephrotoxicity. Materials and Methods: B6 male mice were treated with the CB1 antagonists AM281 or rimonabant and a single dose of cisplatin at 20 mg/kg intraperitoneally. Kidney function,

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Past, Present and Future of Thin Slab Casting ＆ Rolling

Past, Present and Future of Thin Slab Casting ＆ Rolling

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2006年薄板坯连铸连轧国际研讨会

作者： B.mukhopadhyay S.Roychoudhury M.N. Dastur ＆ Company （P） Ltd Kolkata India M.N. Dastur ＆ Company （P） Ltd Kolkata India

Increased competition in the iron and steel sector coupled with the requirement to reduce investment and operating costs have led to the development of new technologies for production of semi-finished

关键词： thin slab casting and rolling conventional process solidification strip quality

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QoS Based Framework for Effective Web Services in Cloud Computing

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Journal of Software Engineering and Applications 2012年第11期5卷 952-960页

作者： Debajyoti mukhopadhyay Falguni J. Chathly Nagesh N. Jadhav Department of Information Technology Maharashtra Institute of Technology Pune India

Enhancements in technology always follow Consumer requirements. Consumer requires best of service with least possible mismatch and on time. Numerous applications available today are based on Web Services and Cloud Computing. Recently, there exist many Web Services with similar functional characteristics. Choosing “a-right” Service from group of similar Web Service is a complicated task for Service Consumer. In that case, Service Consumer can discover the required Web Service using non functional attributes of the Web Services such as QoS. Proposed layered architecture and Web Service-Cloud, i.e. WS-Cloud computing Framework synthesizes the non functional attributes that includes reliability, availability, response time, latency etc. The Service Consumer is projected to provide the QoS requirements as part of Service discovery query. This framework will discover and filter the Web Services form the cloud and rank them according to Service Consumer preferences to facilitate Service on time.

关键词： UDDI SOAP Messages TModel Cloud Computing Web Services Web Service Discovery Information Retrieval Web Discovery Agent

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A Hybrid Web Recommendation System Based on the Improved Association Rule Mining Algorithm

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Journal of Software Engineering and Applications 2013年第8期6卷 396-404页

作者： Ujwala H. Wanaskar Sheetal R. Vij Debajyoti mukhopadhyay Department of Computer Engineering Maharashtra Institute of Technology Pune India Department of Computer Engineering Padmabhooshan Vasantdata Patil Institute of Technology Pune India Department of Information Technology Maharashtra Institute of Technology Pune India

As the growing interest of web recommendation systems those are applied to deliver customized data for their users, we started working on this system. Generally the recommendation systems are divided into two major categories such as collaborative recommendation system and content based recommendation system. In case of collaborative recommendation systems, these try to seek out users who share same tastes that of given user as well as recommends the websites according to the liking given user. Whereas the content based recommendation systems tries to recommend web sites similar to those web sites the user has liked. In the recent research we found that the efficient technique based on association rule mining algorithm is proposed in order to solve the problem of web page recommendation. Major problem of the same is that the web pages are given equal importance. Here the importance of pages changes according to the frequency of visiting the web page as well as amount of time user spends on that page. Also recommendation of newly added web pages or the pages that are not yet visited by users is not included in the recommendation set. To overcome this problem, we have used the web usage log in the adaptive association rule based web mining where the association rules were applied to personalization. This algorithm was purely based on the Apriori data mining algorithm in order to generate the association rules. However this method also suffers from some unavoidable drawbacks. In this paper we are presenting and investigating the new approach based on weighted Association Rule Mining Algorithm and text mining. This is improved algorithm which adds semantic knowledge to the results, has more efficiency and hence gives better quality and performances as compared to existing approaches.

关键词： Web Recommender System Association Rules Web Mining Text Mining

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Tissue engineered indigenous pericardial patch urethroplasty: A promising solution to a nagging problem

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Asian Journal of Urology 2020年第1期7卷 56-60页

作者： Tapan K.Mandal Shashanka Dhanuka Sunirmal Choudhury Bibhas C.mukhopadhyay Ankit Kayal Tapas K.Majhi Maharaj Mondal Department of Urology Nilratan Sircar Medical College and HospitalKolkataIndia

Objective:Urethral stricture is a highly prevalent disease and has a continued ris-ing *** global burden of disease keeps rising as there are significant rates of recur-rence with the existing management options with the need for additional repeat ***,the existing treatment options are associated with significant morbidity in the *** segment urethral strictures are most commonly managed by augmentation *** explored the potential for the application of an acellular tissue engineered bovine pericardial patch in augmentation urethroplasty in a series of our patients suffering from ure-thral stricture *** decreased morbidity due to the avoidance of harvest of buccal mu-cosa,decreased operative time and satisfactory postoperative results make it a promising option for augmentation ***:Nine patients with long segment anterior urethral strictures(involving penile and/or bulbar urethra and stricture length>4 cm)were included in the study after proper informed consent was *** tissue engineered indigenous bovine pericardial patch was used for urethroplasty using dorsal onlay ***:A total of nine patients underwent tissue engineered indigenous pericardial patch ur-ethroplasty for long segment urethral strictures,mostly catheter injury induced or associated with balanitis xerotica *** follow-up was 8 months(range:2-12 months).Out of nine patients,eight(88.9%)were classifed as success and one(11.1%)was classified as ***:Our study brings a product of tissue engineering,already being used in the cardio-vascular surgery domain,into the urological surgery operating room with satisfactory results achieved using standard operating techniques of one stage urethroplasty.

关键词： Stricture urethra Urethroplasty Bovine pericardial patch Tissue engineering Long segment

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