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Visualization of cristae and mtDNA interactions via STED nanoscopy using a low saturation power probe

Light(Science & Applications) 2024年第9期13卷 1741-1756页

作者： Wei Ren Xichuan Ge Meiqi Li Jing Sun Shiyi Li Shu gao Chunyan Shan baoxiang gao Peng Xi Department of Biomedical Engineering National Biomedical Imaging CenterCollege of Future TechnologyPeking UniversityBeijing 100871China Key Laboratory of Analytical Science and Technology of Hebei Province College of Chemistry and Material ScienceHebei UniversityBaoding 071002China School of Life Sciences Peking UniversityBeijing 100871China National Center for Protein Sciences Peking UniversityBeijing 100871China

Mitochondria are crucial organelles closely associated with cellular metabolism and *** DNA(mtDNA)encodes a variety of transcripts and proteins essential for cellular ***,the interaction between the inner membrane(IM)and mtDNA remains elusive due to the limitations in spatiotemporal resolution offered by conventional microscopy and the absence of suitable in vivo probes specifically targeting the ***,we have developed a novel fluorescence probe called HBmito Crimson,characterized by exceptional photostability,fluorogenicity within lipid membranes,and low saturation *** successfully achieved over 500 frames of lowpower stimulated emission depletion microscopy(STED)imaging to visualize the IM dynamics,with a spatial resolution of 40 *** utilizing dual-color imaging of the IM and mtDNA,it has been uncovered that mtDNA tends to habitat at mitochondrial tips or branch points,exhibiting an overall spatially uniform ***,the dynamics of mitochondria are intricately associated with the positioning of mtDNA,and fusion consistently occurs in close proximity to mtDNA to minimize pressure during cristae *** healthy cells,>66%of the mitochondria are Class III(i.e.,mitochondria>5μm or with>12 cristae),while it dropped to<18%in *** dynamics,orchestrated by cristae remodeling,foster the even distribution of ***,in conditions of apoptosis and ferroptosis where the cristae structure is compromised,mtDNA distribution becomes *** findings,achieved with unprecedented spatiotemporal resolution,reveal the intricate interplay between cristae and mtDNA and provide insights into the driving forces behind mtDNA distribution.

关键词： metabolism saturation utilizing

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New Soluble Near-Infrared Dyes Based on Terrylene Diimides with Four Aromatic Heterocycles

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Chinese Journal of Chemistry 2014年第4期32卷 356-360页

作者： Huimin Wei Nan Jiang Nan Zhao Ying Zhang baoxiang gao College of Chemistry and Environmental Science Hebei UniversityBaodingHebei 071002China Key Laboratory of Medicinal Chemistry and Molecular Diagnosis Hebei UniversityBaodingHebei 071002China

Terrylene diimides with four aromatic heterocycles(AHTDIs)were synthesized under Stille Cross-coupling conditions and fully characterized by NMR and mass *** aggregation of the terrylene diimide(TDI)was suppressed by four heterocycles substituents on the bay region,and these AHTDIs exhibited good solubility in common organic *** effects of the substituted groups on the optical and electrochemical properties were also *** introduction of four aromatic heterocycles on the bay of TDI resulted in significant red-shifts of the absorption peak(100 nm),corresponding to a decrease in the band gap from 1.82 to 1.50 ***,with four rich electron aromatic heterocycles,the AHTDIs showed 280 mV negative-shifts of first oxidation poten-tials and a new oxidation wave,corresponding to an increase in the HOMO levels from−5.60 to−5.28 eV.

关键词： terrylene diimides aromatic heterocycles near-infrared dyes optoelectronic properties

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Synthesis of Pyrene-based Planar Conjugated Polymers and the Regioisomers by Intramolecular Cyclization

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中国化学:英文版 2015年第4期33卷 431-440页

作者： Xiaoyan Zhu Yonggang Wu Lina Zhou Yuan Wang Hongchi Zhao baoxiang gao Xinwu Ba College of Chemistry and Environmental Science Hebei UniversityBaodingHebei 071002China

Several planar conjugated polymers and model compounds containing pyrene units are *** pre-cursor polymers and model compounds are obtained through the Pd(0)-catalyzed Suzuki reaction,then the planar structure is formed through intramolecular cyclization in the presence of *** investigate the regioisomers in intramolecular cyclization reaction by using model compounds,suggesting that the syn-and anti-structures are formed in ladder-type polymer LP1,and the syn-structure is more easily formed in *** planar conjugated polymers and model compounds show untypical features of ladder-type polymers,they display broad absorption and blue or blue-green emission in solution,and interchainπ-πstacking in film still ***,the new polymers and model compounds are characterized by nuclear magnetic resonance(NMR)spectroscopy,gel-permeation chromatography(GPC)and cyclic voltammetry(CV).

关键词： planar conjugated polymer pyrene units ladder-type polymer regioisomers defects

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High Brightness and Stability of Si-rhodamine for Super-Resolution Imaging of Mitochondria in Living Cells

High Brightness and Stability of Si-rhodamine for Super-Reso...

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第十一届全国化学生物学学术会议

作者： Xue Zhang Shiyi Li Yifang Song Zixin Shen Wei Yang Xinwei Li baoxiang gao Key Laboratory of Medicinal Chemistry and Molecular Diagnosis （Ministry of Education） College of Chemistry and Environment ScienceHebei University

Super-resolution techniques have far surpassed the diffraction of light microscopes limit and allow observes the details of organelle structure, function, and dynamics. [1] Far-red to near-infrared（NIR） fluorescence probes are expected to be superior because the light in this wavelength region shows greater tissue penetration, has less overlap with the spectrum of background autofluorescence, and exhibits less phototoxicity. [2] Thus, it is highly desired to develop fluorophores that display bright NIR emission with high stability in super-resolution imaging. Here, we report the NIR emission Si-rhodamine dyes（SiRMO） with the methoxy groups on ortho-position of benzene moiety. The SiRMO dyes possess high brightness, and photostability under one-photon excitation, as well as two-photon excitation. Super-resolution imaging with SiRMO dyes enable long-term nanoscopy of the dynamics substructure of mitochondria in live cells. Using SiRMO dyes as two-photon probes, we can visualize mitochondria of neurons in live brain tissue by two-photon fluorescence microscopy.

关键词： Brightness photostability Super-resolution imaging Mitochondria

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Record-high efficiency perovskite LEDs achieved by accelerating radiative recombination

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Science China Materials 2024年第9期67卷 3039-3040页

作者： Feng gao Department of Physics Chemistry and BiologyLinköping UniversityLinköping58183Sweden

Light-emitting diodes(LEDs)have revolutionized the lighting and displays due to their superior energy *** increasing demand for more efficient and brighter thin-film LEDs in flat-panel displays and solid-state lighting applications has driven extensive research into perovskite materials,which show great promise for high brightness and efficiency.

关键词： lighting radiative high

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Stress tensor determination by modified hydraulic tests on pre-existing fractures:Method and stress constraints

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Journal of Rock Mechanics and Geotechnical Engineering 2024年第5期16卷 1637-1648页

作者： Guiyun gao Chenghu Wang Ke gao National Institute of Natural Hazards Ministry of Emergency Management of ChinaBeijing100085China Beijing Engineering Research Center of Earthquake Observation Beijing100085China Key Laboratory of Compound and Chained Natural Hazards Dynamics Ministry of Emergency Management of ChinaBeijing100085China Department of Earth and Space Sciences Southern University of Science and TechnologyShenzhen518055China

The hydraulic testing of pre-existing fractures(HTPF)is one of the most promising in situ stress measurement methods,particularly for three-dimensional stress tensor ***,the stress tensor determination based on the HTPF method requires at least six tests or a minimum of 14-15 tests(under different conditions)for reliable *** this study,we modified the HTPF method by considering the shear stress on each pre-existing fracture,which increased the number of equations for the stress tensor determination and decreased the number of tests *** shear stresses were attributed to different fractures by random sampling;therefore,the stress tensors were obtained by searching for the optimal solution using the least squares criterion based on the Monte Carlo ***,we constrained the stress tensor based on the tensile strength criterion,compressive strength criterion,and vertical stress *** inverted stress tensors were presented and analyzed based on the tensorial nature of the stress using the Euclidean mean stress *** stress-measurement campaigns in Weifang(Shandong Province,China)and Mercantour road tunnel(France)were implemented to highlight the validity and efficiency of the modified HTPF(M-HTPF)*** results showed that the M-HTPF method can be applied for stress tensor inversion using only three to four tests on pre-existing fractures,neglecting the stress *** inversion results were confined to relatively small distribution dispersions and were significantly reliable and stable due to the shear stresses on the fractures and the stress constraints *** M-HTPF method is highly feasible and efficient for complete stress tensor determination in a single borehole.

关键词： Stress tensor Hydraulic tests on pre-existing fractures Mean stress Stress constraint Hydraulic fracturing

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Thermal and mechanical behavior of casting copper alloy wheel during wheel and belt continuous casting process

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China Foundry 2024年第1期21卷 82-90页

作者： Kun gao Yan Peng School of Mechanical Engineering Yanshan UniversityQinhuangdao 066004HebeiChina

To investigate the thermal and mechanical behavior of casting wheel,a two-dimensional thermoelastic-plastic finite element model was used to predict the temperature,stress and distortion distribution of the casting wheel during the wheel and belt continuous casting *** effects of grinding thickness and casting speed on the thermal and mechanical behaviors of the center of the hot face of the casting wheel were discussed in *** each rotation,the casting wheel passes through four different spray *** results show that the temperature distribution of the casting wheel in different spray zones is similar,the temperature of the hot face is the highest and the temperature reaches the peak in the spray zoneⅢ.The stress and distortion depend on the temperature distribution,and the maximum stress and distortion of the hot face are 358.2 MPa and 1.82 mm,*** temperature at the center of the hot face decreases with increasing grinding thickness and increases with increasing casting speed.

关键词： casting wheel finite element model grinding thickness casting speed hot face spray zones

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Numerical simulation of fluid flow and free surface fluctuations during wheel and belt casting process

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Journal of Iron and Steel Research International 2024年第5期31卷 1117-1126页

作者： Kun gao Yan Peng National Engineering Research Center for Equipment and Technology of Cold Strip Rolling Yanshan UniversityQinhuangdao066004HebeiChina

The unstable fluid flow and severe free surface fluctuations in the wheel and belt caster can affect the quality of the cast *** lower level height tends to entrap inclusions in the molten *** the other hand,the higher level height makes the production process more dangerous due to the overflow of high temperature fluid from the mold.A computational model of the molten metal pouring process was *** transient fluid flow and free surface fluctuations behavior were calculated using the three-dimensional large eddy simulation model and the volume of fluid *** results show that the flow velocity of the main jet gradually decreases under the influence of the low kinetic energy fluid in the *** is an obvious oscillation in the tail of the jet,while the flow field is asymmetric in *** jet is closer to the inside radius side due to the Coanda effect,and there is a recirculation zone on the inside radius and the outside radius respectively,according to the 10 s time-averaged *** with the industrial observation and simulation results,the shape of the free surface is a wave that varies with *** addition,the free surface height is lowest and the flow velocity is highest in the region near the jet.

关键词： Fluid flow Free surface Wheel and belt caster Large eddy simulation Volume of fluid method

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On the core entropy of Newton maps

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Science China Mathematics 2024年第1期67卷 77-128页

作者： Yan gao Department of Mathematics Shenzhen UniversityShenzhen 518060China

In this paper,we define the core entropy for postcritically-finite Newton maps and study its continuity within this *** show that the entropy function is not continuous in this family,which is different from the polyn... 详细信息

关键词： core entropy extended Newton graph critical marking polynomial Newton map

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Durable poly(binaphthyl-co-p-terphenyl piperidinium)-based anion exchange membranes with dual side chains

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Journal of Energy Chemistry 2024年第2期89卷 324-335,I0008页

作者： Weiting gao Xuelang gao Qiugen Zhang Aimei Zhu Qinglin Liu Department of Chemical&Biochemical Engineering Fujian Provincial Key Laboratory of Theoretical and Computational ChemistryThe College of Chemistry and Chemical EngineeringXiamen UniversityXiamen 361005FujianChina Department of Chemistry Pohang University of Science and Technology(POSTECH)Pohang 790-784South Korea

Building well-developed ion-conductive highways is highly desirable for anion exchange membranes(AEMs).Grafting side chain is a highly effective approach for constructing a well-defined phaseseparated morphological structure and forming unblocked ion pathways in AEMs for fast ion *** of side chains can further enhance phase separation due to the superhydrophobic nature of fluorine ***,their electronic effect on the alkaline stability of side chains and membranes is rarely ***,fluorine-containing and fluorine-free side chains are introduced into the polyaromatic backbone in proper configuration to investigate the impact of the fluorine terminal group on the stability of the side chains and membrane *** poly(binaphthyl-co-p-terphenyl piperidinium)AEM(QBNp TP)has the highest molecular weight and most dimensional stability due to its favorable backbone arrangement among ortho-and meta-terphenyl based ***,by introducing both a fluorinated piperidinium side chain and a hexane chain into the p-terphenyl-based backbone,the prepared AEM(QBNp TP-QFC)presents an enhanced conductivity(150.6 m S cm^(-1))and a constrained swelling at 80℃.The electronic effect of fluorinated side chains is contemplated by experiments and *** results demonstrate that the presence of strong electro-withdrawing fluorine groups weakens the electronic cloud of adjacent C atoms,increasing OH^(-)attack on the C atom and improving the stability of piperidinium *** QBNp TP-QFC possesses a robust alkaline stability at 80℃(95.3%conductivity retention after testing in 2 M Na OH for 2160 h).An excellent peak power density of 1.44 W cm^(-2)and a remarkable durability at 80℃(4.5%voltage loss after 100 h)can be observed.

关键词： Anion exchange membranes Backbone structure Fluorinated side chain Alkaline stability

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