Visualization of cristae and mtDNA interactions via STED nanoscopy using a low saturation power probe
作者机构：Department of Biomedical EngineeringNational Biomedical Imaging CenterCollege of Future TechnologyPeking UniversityBeijing 100871China Key Laboratory of Analytical Science and Technology of Hebei ProvinceCollege of Chemistry and Material ScienceHebei UniversityBaoding 071002China School of Life SciencesPeking UniversityBeijing 100871China National Center for Protein SciencesPeking UniversityBeijing 100871China
出 版 物：《Light(Science & Applications)》 (光（科学与应用)（英文版）)
年 卷 期：2024年第13卷第9期
页 面：1741-1756页
核心收录：
基 金：supported by the National Key R&D Program of China(2022YFC3401100) National Natural Science Foundation of China(22177024,62025501,31971376,92150301)
主 题：metabolism saturation utilizing
摘 要：Mitochondria are crucial organelles closely associated with cellular metabolism and *** DNA(mtDNA)encodes a variety of transcripts and proteins essential for cellular ***,the interaction between the inner membrane(IM)and mtDNA remains elusive due to the limitations in spatiotemporal resolution offered by conventional microscopy and the absence of suitable in vivo probes specifically targeting the ***,we have developed a novel fluorescence probe called HBmito Crimson,characterized by exceptional photostability,fluorogenicity within lipid membranes,and low saturation *** successfully achieved over 500 frames of lowpower stimulated emission depletion microscopy(STED)imaging to visualize the IM dynamics,with a spatial resolution of 40 *** utilizing dual-color imaging of the IM and mtDNA,it has been uncovered that mtDNA tends to habitat at mitochondrial tips or branch points,exhibiting an overall spatially uniform ***,the dynamics of mitochondria are intricately associated with the positioning of mtDNA,and fusion consistently occurs in close proximity to mtDNA to minimize pressure during cristae *** healthy cells,66%of the mitochondria are Class III(i.e.,mitochondria5μm or with12 cristae),while it dropped to18%in *** dynamics,orchestrated by cristae remodeling,foster the even distribution of ***,in conditions of apoptosis and ferroptosis where the cristae structure is compromised,mtDNA distribution becomes *** findings,achieved with unprecedented spatiotemporal resolution,reveal the intricate interplay between cristae and mtDNA and provide insights into the driving forces behind mtDNA distribution.
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