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引入MobileNet的轻量化森林火灾视频监测方法研究

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微型电脑应用 2024年第5期40卷 5-8页

作者：刘燕吴宇兴赵俊杰程宝平汪胜中移(杭州)信息技术有限公司浙江杭州311100

应用视频火灾检测技术能够有效地提高森林火灾监测和预警能力,在生态保护和社会公共安全方面具有重要意义,为此研究适合森林火灾检测的图像静态、动态特征检测方法。同时,使卷积神经网络YOLO轻量化,引入GAM在准确率、模型尺寸和速率上... 详细信息

应用视频火灾检测技术能够有效地提高森林火灾监测和预警能力,在生态保护和社会公共安全方面具有重要意义,为此研究适合森林火灾检测的图像静态、动态特征检测方法。同时,使卷积神经网络YOLO轻量化,引入GAM在准确率、模型尺寸和速率上进行平衡。优化的模型在牺牲准确率1.9%的情况下,参数量降低约80%,准确率在自制森林火灾数据集上达到92.4%。这一种基于颜色-运动-机器学习技术相结合的火灾监测新方法总体轻量精简,对实时火灾监测系统设计具有参考价值。

关键词：视频火灾检测颜色模型运动检测卷积神经网络全局注意力机制

Green fluorescent protein (GFP) as a vital marker for studying the interaction of Phytophthora sojae and soybean

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Chinese Science Bulletin 2009年第16期54卷 2822-2829页

作者： CHEN XiaoRen cheng baoping WANG XinLe DONG SuoMeng WANG YongLin ZHENG XiaoBo WANG YuanChao Department of Plant Pathology Nanjing Agricultural University Nanjing 210095 China

Transgenic Phytophthora sojae strains that produce green fluorescent protein (GFP) were obtained after stable DNA integration using the Hsp70 promoter and the Ham34 terminator of Bremia lactucae. The expression of GFP during different developmental stages of P. sojae was observed using fluorescent microscopy. Based on this reporter system, the histopathologic events caused by the pathogen in soybean leaves, hypocotyls and roots were monitored. Meanwhile, the difference in resistance between different soybean cultivars against P. sojae was analyzed microscopically in roots. The results indicate that GFP can be stably expressed in zoosporangia, zoospores, cysts, hyphae and oospores of P. sojae. Using the GFP marker, the infecting pathogens in leaves, hypocotyls and roots of host could be distinctly visualized. The germ tube length of cysts germinating on the roots of resistant cultivar Nannong 8848 was longer than that on the roots of susceptible cultivar Hefeng 35. These results show for the first time that this eukaryotic reporter can be used in P. sojae as a stable and vital marker, allowing the study of genetics of this hemibiotrophic pathogen.

关键词：绿色荧光蛋白标记大豆疫霉根腐病 GFP 大豆疫霉菌热休克蛋白70 显微镜观察大豆叶片 DNA整合

评估茶枝柑黄龙病病情的3种定量方法的比较研究

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生物灾害科学 2024年第1期47卷 73-80页

作者：马冲何桢锐孔广辉周江文钟君泽周而勋程保平华南农业大学植物保护学院/广东省微生物信号与作物病害防控重点实验室广东广州510642 广东省农业科学院植物保护研究所/农业农村部华南果蔬绿色防控重点实验室/广东省植物保护新技术重点实验室广东广州510640 广东省江门市广盈陈皮柑普茶有限公司广东江门529020

【目的】为比较3种不同黄龙病病情定量评估方法的优劣之处。【方法】运用实时荧光定量PCR(q RT-PCR)技术,定量分析了柑橘黄龙病菌(Candidatus Liberibacter asiaticus,CLas)在茶枝柑的叶脉、叶肉、果皮、囊壁、果实中柱、树皮、根系等... 详细信息

【目的】为比较3种不同黄龙病病情定量评估方法的优劣之处。【方法】运用实时荧光定量PCR(q RT-PCR)技术,定量分析了柑橘黄龙病菌(Candidatus Liberibacter asiaticus,CLas)在茶枝柑的叶脉、叶肉、果皮、囊壁、果实中柱、树皮、根系等多个部位的分布情况,并进一步使用“橘园黄龙病病情指数分析、病树中CLas含量分析及黄龙病发病率检测”3种方法定量评估了新会区两个茶枝柑果园中黄龙病的发病情况。【结果】CLas在茶枝柑各部位含量的大小:叶脉>橘络>果实中柱>根>叶肉>绿树皮>果皮白瓤>囊壁>外果皮。茶枝柑叶脉中CLas 16S rDNA基因平均含量为10^(7.89)CN/g FW,茶枝柑外果皮中CLas 16S rDNA基因平均含量为10^(4.54)CN/g FW。三江镇联合村果园20棵茶枝柑的平均病情指数为52.75;随机采集叶片样品,可在其中18棵茶枝柑的叶脉样品中检出CLas,其16S rDNA平均含量为10^(5.57)CN/g FW;选择性采集显症叶片样品,可检测出20棵茶枝柑树的黄龙病发病率为100%。双水镇大树村果园20棵茶枝柑的平均病情指数为30.75;随机采集叶片样品,可在其中3棵茶枝柑的叶脉样品中检出CLas,其16S rDNA基因的平均含量为10^(4.99)CN/g FW;选择性采集显症叶片样品,可检测出20棵茶枝树的黄龙病发病率为100%。【结论】明确CLas在茶枝柑不同部位的定量分布情况,为茶枝柑的黄龙病定量分析提供了参考依据;3种定量评估茶枝柑黄龙病病情的方法各有优劣,综合运用才能更准确全面地反映出黄龙病在果园的发病情况。

关键词：柑橘黄龙病茶枝柑病情指数发病率柑橘黄龙病菌含量

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青蛤半人工育苗技术

中国水产 2002年第4期 61-63页

作者：程宝平河北省乐亭县水产局 063600

青蛤[Cylina sinensis(Gmelin)]是生活于潮间滩涂的埋栖型双壳贝类,从80年代以来,我国从南到北开始进行青蛤投苗养殖.由于青蛤人工育苗一直未成功,依赖的自然苗种紧缺,成为限制青蛤养殖生产发展的主要因素.

关键词：育苗技术青蛤半人工育苗

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深圳赛格广场地下室全逆作法施工技术

施工技术 1999年第8期28卷 6-7,21页

作者：程宝坪中国建筑二局深圳南方建筑公司

介绍深圳赛格广场工程地下室全逆作法施工中深基坑支护、土方开挖及地下室结构工程施工，分析此方法在改善施工条件、缩短工期、节约深基坑支护费用等方面的优势。

关键词：高层建筑地下室全逆作法商业广场

Identification and characterization of Phytopythium helicoids causing stem rot of ’Shatangju’ mandarin seedlings in China

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Identification and characterization of Phytopythium helicoid...

中国植物病理学会第十二届青年学术研讨会

作者： Xiaoren Chen Beibei Liu Yuping Xing baoping cheng Yunhui Tong Jingyou Xu College of Horticulture and Plant Protection Yangzhou University Plant Protection Research Institute Guangdong Academy of Agricultural Sciences

Citrus mandarin（Citrus reticulata） cultivar ’Shatangju’ possesses great economic importance in southern China because of its delicious fruits and medicinal *** surveys conducted in 2012 and 2013,a stem rot disease were observed on grafted ’Shatangju’ seedlings in 21 nurseries located in three regions（Yangjiang City,Sihui City and Deqing County） of Guangdong Province,*** aim of this study was to characterize the symptoms of seedling stem rot;evaluate the incidence and loss;identify the causal agent and evaluate its pathogenicity and *** disease was present in all surveyed nurseries but incidence varied with location.A Pythium-like oomycete species was consistently obtained from symptomatic plants collected in the nurseries *** the basis of morphological and cultural characteristics and combined phylogenetic analysis of four molecular barcodes including the internal transcribed spacer region（ITS）,β-tubulin,mitochondrial CoxⅠ and CoxⅡgenes,20 isolates obtained were identified as belonging to the species Phytopythium *** representative isolates were pathogenic to the scions but rootstocks of’Shatangju’ mandarin seedlings cultivated under nursery conditions and in a greenhouse and symptoms identical to that observed in the field were *** pathogen was reisolated consistently from the inoculated plants,confirming Koch’s *** stem rot and death caused by *** represents a serious threat to ’Shatangju’ mandarin nurseries productivity and longevity in southern China.

关键词： ’Shatangju’ mandarin stem rot Phytopythium helicoids molecular barcode pathogenicity

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坚持传统浑然天成——记学书点滴

现代艺术 2020年第1期 92-93页

作者：程保平四川省书法家协会四川省草书研究会成都市书法家协会成都丙戌金石书画研究会

从小就喜欢写写画画,上小学时,经常把课本的背面画满各种枪炮或看过的电影人物,遭老师批评。上中学后开始写毛笔字,在一位老先生的指导下第一次临习了颜真卿的《多宝塔感应碑》,这种写写画画的喜好一直伴随着我参加工作,也正由于此,鬼... 详细信息

从小就喜欢写写画画,上小学时,经常把课本的背面画满各种枪炮或看过的电影人物,遭老师批评。上中学后开始写毛笔字,在一位老先生的指导下第一次临习了颜真卿的《多宝塔感应碑》,这种写写画画的喜好一直伴随着我参加工作,也正由于此,鬼使神差的被单位安排到工会做宣传工作,此后又到了宣传部门。1978年至1980年,参加了成都市工会举办的职工业余美术培训班,三年时间,学习了素描、水粉和油画,老师是何哲生先生。那时,因经常画板报、出专刊,也常写毛笔字,但说不上书法。

关键词：宣传部门多宝塔感应碑老师颜真卿浑然天成美术培训班

Development of a Loop-mediated Isothermal Amplification Assay for the Rapid Detection of the Overlooked Oomycete Phytopathogen Phytopythium helicoides

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Development of a Loop-mediated Isothermal Amplification Assa...

中国植物病理学会2023年学术年会

作者： WEN Ke ZHAI Xinyu ZHOU Xue LIU Yang JIN Jinghao cheng baoping SRIVASTAVA Vaibhav CHEN Xiaoren College of Plant Protection Yangzhou University

The phytopathogenic oomycete Phytopythium helicoides,formerly known as Pythium helicoides,is a newly described threat to citrus cultivar Shatangju(Citrus reticulata(***) in southern *** detect *** for the rapid diagnosis and control Shatangju leaf blight and stem rot that it causes,we developed a loop-mediated isothermal amplification(LAMP) assay that targets the β-tubulin gene region of *** a set of screened primers and optimized reaction conditions of 60℃ for 70 min,the LAMP assay can detect specifically the pathogen from all the tested phytopathogens that are associated with *** assay consistently showed high sensitivity in detecting low contents of the pathogen DNA(30 pg),which is comparable to quantitative polymerase chain reaction *** confirmed the practical application of this LAMP assay in diagnosing both citrus leaves and strawberry crowns infected by this *** LAMP method developed in this study provides a specific,sensitive,and rapid detection of *** to assist in the control of Shatangju leaf blight and stem rot,as well as other crop diseases associated with this pathogen,such as strawberry crown rot.

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SPWM的谐波及抑制

电气技术 2010年第6期11卷 47-49页

作者：程宝平山西长治市应用技术研究院山西长治046011

本文对SPWM的谐波及其分布规律进行了分析,讨论了SPWM逆变器的主要参数对谐波频谱变化的影响,归纳了工程实际中采用的几种谐波抑制方法。

关键词： SPWM 载波频率谐波注入法指定谐波抑制法趋近采样法低通滤波器