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Generation of Eco-Friendly and Disease-Resistant Channel Catfish(Ictalurus punctatus)Harboring the Alligator Cathelicidin Gene via CRISPR/Cas9 Engineering

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Engineering 2024年第8期39卷 273-286页

作者： Jinhai Wang baofeng su De Xing Timothy J.Bruce Shangjia Li Logan Bern Mei Shang Andrew Johnson Rhoda Mae C.Simora Michael Coogan Darshika U.Hettiarachchi Wenwen Wang Tasnuba Hasin Jacob Al-Armanazi Cuiyu Lu Rex A.Dunham School of Fisheries Aquacultureand Aquatic SciencesAuburn UniversityAuburnAL 36849USA

As a precise and versatile tool for genome manipulation,the clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)platform holds promise for modifying fish traits of *** the aim of reducing transgene introgression and controlling reproduction,upscaled disease resistance and reproductive intervention in catfish species have been studied to lower the potential environmental risks of the introgression of escapees as transgenic *** advantage of the CRISPR/Cas9-mediated system,we succeeded in integrating the cathelicidin gene(As-Cath)from an alligator(Alligator sinensis)into the target luteinizing hormone(lh)locus of channel catfish(Ictalurus punctatus)using two delivery systems assisted by double-stranded DNA(dsDNA)and single-stranded oligodeoxynucleotides(ssODNs),*** this study,high knock in(KI)efficiency(22.38%,64/286)but low ontarget events was achieved using the ssODN strategy,whereas adopting a dsDNA as the donor template led to an efficient on-target KI(10.80%,23/213).The on-target KI of As-Cath was instrumental in establishing the lh knockout(LH^(–)_As-Cath^(+))catfish line,which displayed heightened disease resistance and reduced fecundity compared with the wild-type(WT)sibling ***,administration of human chorionic gonadotropin(HCG)and luteinizing hormone-releasing hormone analogue(LHRHa)can restore the reproduction of the transgenic fish ***,we replaced the lh gene with an alligator cathelicidin transgene and then administered hormone therapy to move towards complete reproductive control of diseaseresistant transgenic catfish in an environmentally responsible *** strategy not only effectively improves consumer-valued traits but also guards against unwanted introgression,providing a breakthrough in aquaculture genetics to confine fish reproduction and prevent the establishment of transgenic or domestic genotypes in the natural environment.

关键词： Genome editing ssODN dsDNA Antimicrobial peptide Reproductive confinement Aquaculture

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基于时间序列植被指数的小麦条锈病抗性等级鉴定方法

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农业工程学报 2024年第4期40卷 155-165页

作者：苏宝峰刘砥柱陈启帆韩德俊吴建辉西北农林科技大学机械与电子工程学院杨凌712100 农业农村部农业物联网重点实验室杨凌712100 陕西省农业信息感知与智能服务重点实验室杨凌712100 西北农林科技大学农学院杨凌712100 西北农林科技大学旱区作物逆境生物学国家重点实验室杨凌712100

条锈病严重影响小麦产量,培育抗条锈病的小麦品种至关重要。针对传统育种中抗性鉴定手段单一、效率低的问题,该研究提出了一种通过小麦冠层植被指数的时间序列实现对条锈病不同抗性等级的高效鉴定方法。该方法利用无人机采集自然发病的... 详细信息

条锈病严重影响小麦产量,培育抗条锈病的小麦品种至关重要。针对传统育种中抗性鉴定手段单一、效率低的问题,该研究提出了一种通过小麦冠层植被指数的时间序列实现对条锈病不同抗性等级的高效鉴定方法。该方法利用无人机采集自然发病的育种群体小麦(共600个样本,516个基因型)冠层多时相的光谱图像,使用随机蛙跳算法和ReliefF算法筛选出6个条锈病病害严重度的敏感特征:归一化色素叶绿素指数(normalized pigment chlorophyll index,NPCI)、沃尔贝克指数(woebbecke index,WI)、叶绿素红边指数(chlorophyll index rededge,CIrededge)、绿大气抵抗植被指数(green atmospherically resistant index,GARI)、归一化差分植被指数(normalized difference vi,NDVI)、叶绿素绿指数(chlorophyll index green,CIgreen),这些敏感特征在试验群体中的时间序列符合条锈病的发病规律,验证了其作为条锈病发病严重度敏感特征的有效性;基于支持向量机(support vector machine,SVM)算法使用上述敏感特征建立条锈病病害严重度等级分类模型,在测试集的表现中,与使用未经过筛选的原始特征所建立的模型相比在精度、平均准确率、平均召回率和F1分数上分别仅下降6.2%、3.3%、2.7%、4.0%,证明了所筛选敏感特征的有效性;针对一般机器学习算法难以捕捉不同抗性等级样本之间较小的特征变化差异的问题,提出了一种从植被指数时间序列转化生成的二维图像中提取特征实现条锈病抗性等级分类的方法。将敏感特征中能够较好区分不同抗病等级的4个时间序列植被指数(NPCI、GARI、NDVI、WI),通过格拉姆角场方法生成格拉姆角和场图像,并制作成数据集,使用DenseNet121网络进行训练,以实现不同条锈病抗病等级的分类。建立的条锈病抗性等级分类模型中,由NPCI时间序列图像建立的分类模型测试效果最佳,其准确率为0.837,召回率为0.834,F1分数可达0.833,能够较好地实现对群体小麦不同品种(系)的条锈病抗性等级差异的区分,表明基于光谱植被指数时间序列的小麦条锈病抗性等级识别方法可以用于小麦抗病育种中抗性等级的鉴定,并可为其他作物的病害抗性等级鉴定提供一定的参考。

关键词：无人机遥感机器学习深度学习小麦条锈病多光谱成像 DenseNet121

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Assessment of canopy vigor information from kiwifruit plants based on a digital surface model from unmanned aerial vehicle imagery

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International Journal of Agricultural and Biological Engineering 2019年第1期12卷 165-171页

作者： Jinru Xue Yeman Fan baofeng su Sigfredo Fuentes College of Mechanical and Electronic Engineering Northwest A&F UniversityYangling 712100ShaanxiChina Key Laboratory of Agricultural Internet of Things Ministry of Agriculture and Rural AffairsYangling 712100ShaanxiChina Shaanxi Key Laboratory of Agricultural Information Perception and Intelligent Services Yangling 712100ShaanxiChina School of Agriculture and Food Faculty of Veterinary and Agricultural SciencesUniversity of MelbourneParkville 3010.VictoriaAustralia

Information about canopy vigor and growth are critical to assess the potential impacts of biotic or abiotic stresses on plant *** implementing a Digital surface Model(DSM)to imagery obtained using Unmanned Aerial Vehicles(UAV),it is possible to filter canopy information effectively based on height,which provides an efficient method to discriminate canopy from soil and lower vegetation such as weeds or cover *** paper describes a method based on the DSM to assess canopy growth(CG)as well as missing plants from a kiwifruit orchard on a plant-by-plant *** DSM was initially extracted from the overlapping RGB aerial imagery acquired over the kiwifruit orchard using the Structure from Motion(SfM)*** adaptive threshold algorithm was implemented using the height difference between soil/lower plants and kiwifruit canopies to identify plants and extract canopy information on a non-regular ***,a customized algorithm was developed to discriminate single kiwifruit plants automatically,which allowed the estimation of individual canopy cover fractions(fc).By applying differential fc thresholding,four categories of the CG were determined automatically:(i)missing plants;(ii)low vigor;(iii)moderate vigor;and(iv)*** were validated by a detailed visual inspection on the ground,which rendered an overall accuracy of 89.5%for the method proposed to assess CG at the plant-by-plant ***,the accuracies for CG category(i)-(iv)were 94.1%,85.1%,86.7%,and 88.0%,*** proposed method showed also to be appropriate to filter out weeds and other smaller non-plant materials which are extremely difficult to be distinguished by common colour thresholding or edge identification methods.

关键词： canopy vigor UAV imagery digital surface model kiwifruit plant missing plants photogrammetry plant stress

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Functionality of dietary antimicrobial peptides in aquatic animal health:Multiple meta-analyses

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Animal Nutrition 2023年第1期12卷 200-214页

作者： Jinhai Wang Alan E.Wilson baofeng su Rex A.Dunham School of Fisheries Aquaculture and Aquatic SciencesAuburn University203 Swingle HallAuburnAL 36849USA

Effects of antimicrobial peptides(AMP)added to diets on aquatic animal health and body function are influenced by multiple factors such as animal species,initial body weight,the dosage of AMP and feeding ***,there is limited knowledge on the relationship between these factors and the body function of aquatic ***,we aimed to perform multiple meta-analyses to investigate the effects of dietary AMP on growth performance(feed conversion ratio[FCR],specific growth rate[SGR]),enzyme activity(superoxide dismutase activity[SOD],lysozyme activity[LSA]),disease resistance(cumulative survival rate[CSR],the expression of immune-related genes[GENE])and the abundance of gut microbiota(MICRO)from a pool of empirical ***,the dose-effect model was applied to determine the optimal AMP dose,initial body weight and feeding duration to maximize body *** conduct the meta-analyses,we included 34 publications that estimated 705 effect sizes across 21 fish,2 shrimp and 2 shellfish *** results confirmed that the inclusion of AMP in the diet can significantly improve SGR,SOD,LSA,CSR and GENE and decrease FCR for aquatic ***,our findings implied a slight positive effect of AMP on MICRO albeit with a limited number of studies available on fish gut microbial *** no significant linear or quadratic relationship was predicted by meta-regression,the dose-effect indicated that the optimal AMP doses for FCR,SGR,SOD and LSA were 707.5,750.0,1,050.0 and 937.5 mg/kg,*** together,fish with an initial body weight of 30 g could be fed with a dose of 600 to 800 mg/kg for 2 mo when AMP-supplemented diets were applied in aquaculture,which can effectively improve body function and health while lowering aquafeed *** addition,more studies should address fish gut microbiota to delimitate the influence of dietary AMP on MICRO in the future.

关键词： Fish AMP Oral administration Feed additive Dose-effect Immune

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Foliar application of chelated sugar alcohol calcium fertilizer for regulating the growth and quality of wine grapes

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International Journal of Agricultural and Biological Engineering 2022年第3期15卷 153-158页

作者： Tinghui Ma Yueran Hui Li Zhang baofeng su Rui Wang Ningxia Academy of Agriculture and Forestry Sciences Yinchuan 750002China School of Agriculture Ningxia UniversityYinchuan 750021China College of Mechanical and Electronic Engineering Northwest A&F UniversityYangling 712100ShaanxiChina

The eastern foothills of Helan Mountain are a production area of high-quality wine grapes,but the low content of water-soluble calcium in the alkaline soil in this area has become an important limiting factor for the production of high-end *** this study,7-year-old Cabernet Sauvignon grapes grown at Lilan Winery,which is located at the eastern foot of Helan Mountain in Ningxia,China,were used to examine the effect of exogenous calcium supplementation on fruit growth and berry *** sugar alcohol was applied as a foliar spray at 1.2 L/hm^(2)(T1),2.4 L/hm^(2)(T2),3.6 L/hm^(2)(T3),4.8 L/hm^(2)(T4),and 6.0 L/hm^(2)(T5)concentrations at the fruit expansion stage,the early stage of grape veraison,the middle stage of grape veraison,and the later stage of grape *** control was used for *** results showed that foliar calcium supplementation can significantly enhance the photosynthetic characteristics of wine grape leaves,increase the chlorophyll content of leaves,and improve the quality of grape *** the T3(3.6 L/hm^(2))concentration of exogenous calcium,the transverse diameter,the longitudinal diameter,and fruit shape index of grapes were the highest,which were increased by 1.71%,5.33%,and 3.92%compared to the control,*** application of high calcium concentrations(T4 and T5)inhibited the physiological development of *** spike length,hundred-grain weight,yield of wine grapes,sugar-acid ratio,tannin,and total phenols were the highest in the T2(2.4 L/hm^(2))*** results of principal component analysis based on multiple indicators showed that foliar application of 2.4-3.6 L/hm^(2)chelated sugar alcohol calcium on the leaf surfaces of grape leaves was the most suitable for improving the physiological characteristics and berry quality of wine grapes.

关键词： wine grapes chelated sugar alcohol calcium mineral elements berry quality Helan Mountain

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Effects of regulated deficit irrigation on the growth and berry composition of Cabernet Sauvignon in Ningxia

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International Journal of Agricultural and Biological Engineering 2019年第6期12卷 102-109页

作者： Rui Wang Pengke Yan Quan sun baofeng su Junxiang Zhang School of Agronomy Ningxia UniversityYinchuan 750021China College of Mechanical and Electronic Engineering Northwest A&F UniversityYangling 712100China Ningxia Grapes and Wine Research Institute Yinchuan 750021China

The adoption of water-saving irrigation strategies is required particularly for wine grape variety,which has been widely cultivated in arid and semiarid *** assess vine response to regulated deficit irrigation(RDI),the grape growth and berry composition under five treatments that irrigated at a certain percentage of the crop evapotranspiration(ET c)were evaluated over a 3-year period in a vineyard with the grape variety of Cabernet *** results indicated that RDI had a significant effect on the grape berry size and *** largest berry size(12.20 mm)was obtained under the T50 in 2014,while the smallest berry size(9.83 mm)one was obtained under the CK treatments in the same *** highest individual yield occurred in the T50 treatment,with an average of 1.99 kg,followed by the T25-50 ***,both weights were significantly larger than that of the CK *** with the T50 treatments,the individual grape vine yield in the T50-25 treatments were slightly less by 16.9%for 2013,15.3%for 2014 and 18.1%for *** to control(CK)treatment,the soluble solid and reducing sugar contents decreased,the total acid content increased,and the sugar/acid ratio basically showed a downward *** treatment irrigated at 50%ET c until veraison and 25%thereafter(T50-25)increased the phenolic compound content in grape *** treatment received only rain water during the grape growing season(CK)and the one irrigated at 25%of the ET_(c) crop evapotranspiration(T25)caused defoliation and negatively affected the yields and grape composition during all 3 ***,the RDI not only inhibited the vine vegetative growth but also improved the fruit *** terms of productivity and grape composition,the Cabernet Sauvignon grape variety was most sensitive to water stress *** the comprehensive consideration of yield,water-use efficiency and berry composition,the T50-25 treatment was the most efficient irrigation strategy in t

关键词： regulated deficit irrigation Cabernet Sauvignon arid area grape composition

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Segmentation of field grape bunches via an improved pyramid scene parsing network

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International Journal of Agricultural and Biological Engineering 2021年第6期14卷 185-194页

作者： Shan Chen Yuyang Song Jinya su Yulin Fang Lei Shen Zhiwen Mi baofeng su College of Mechanical and Electronic Engineering Northwest A&F UniversityYangling 712100ShaanxiChina Key Laboratory of Agricultural Internet of Things Ministry of Agriculture and Rural AffairsYangling 712100ShaanxiChina Shaanxi Key Laboratory of Agricultural Information Perception and Intelligent Services Yangling 712100ShaanxiChina College of Enology Northwest A&F UniversityYangling 712100ShaanxiChina School of Computer Science and Electronic Engineering University of EssexColchesterCO43SQUK

With the continuous expansion of wine grape planting areas,the mechanization and intelligence of grape harvesting have gradually become the future development *** order to guide the picking robot to pick grapes more efficiently in the vineyard,this study proposed a grape bunches segmentation method based on Pyramid Scene Parsing Network(PSPNet)deep semantic segmentation network for different varieties of grapes in the natural field *** this end,the Convolutional Block Attention Module(CBAM)attention mechanism and the atrous convolution were first embedded in the backbone feature extraction network of the PSPNet model to improve the feature extraction ***,the proposed model also improved the PSPNet semantic segmentation model by fusing multiple feature layers(with more contextual information)extracted by the backbone *** improved PSPNet was compared against the original PSPNet on a newly collected grape image dataset,and it was shown that the improved PSPNet model had an Intersection-over-Union(IoU)and Pixel Accuracy(PA)of 87.42%and 95.73%,respectively,implying an improvement of 4.36%and 9.95%over the original PSPNet *** improved PSPNet was also compared against the state-of-the-art DeepLab-V3+and U-Net in terms of IoU,PA,computation efficiency and robustness,and showed promising *** is concluded that the improved PSPNet can quickly and accurately segment grape bunches of different varieties in the natural field environments,which provides a certain technical basis for intelligent harvesting by grape picking robots.

关键词： grape bunches semantic segmentation deep learning improved PSPNet

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Digital surface model applied to unmanned aerial vehicle based photogrammetry to assess potential biotic or abiotic effects on grapevine canopies

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International Journal of Agricultural and Biological Engineering 2016年第6期9卷 119-130页

作者： su baofeng Xue Jinru Xie Chunyu Fang Yulin Song Yuyang Sigfredo Fuentes College of Mechanical and Electronic Engineering Northwest A&F UniversityYangling 712100China College of Enology Northwest A&F UniversityYangling 712100China Faculty of Veterinary and Agricultural Sciences University of MelbourneParkville 3010Australia

Accurate data acquisition and analysis to obtain crop canopy information are critical steps to understand plant growth dynamics and to assess the potential impacts of biotic or abiotic stresses on plant development.A versatile and easy to use monitoring system will allow researchers and growers to improve the follow-up management strategies within farms once potential problems have been *** study reviewed existing remote sensing platforms and relevant information applied to crops and specifically grapevines to equip a simple Unmanned Aerial Vehicle(UAV)using a visible high definition RGB *** objective of the proposed Unmanned Aerial System(UAS)was to implement a Digital surface Model(DSM)in order to obtain accurate information about the affected or missing grapevines that can be attributed to potential biotic or abiotic stress *** analysis process started with a three-dimensional(3D)reconstruction from the RGB images collected from grapevines using the UAS and the Structure from Motion(SfM)technique to obtain the DSM applied on a per-plant ***,the DSM was expressed as greyscale images according to the halftone technique to finally extract the information of affected and missing grapevines using computer vision algorithms based on canopy cover measurement and *** validate the automated method proposed,each grapevine row was visually inspected within the study *** inspection was then compared to the digital assessment using the proposed UAS in order to validate calculations of affected and missing grapevines for the whole studied *** showed that the percentage of affected and missing grapevines was 9.5%and 7.3%,respectively from the area ***,for this specific study,the abiotic stress that affected the experimental vineyard(frost)impacted a total of 16.8%of *** study provided a new method for automatically surveying affected or missing grapevines in the field and an evaluation tool fo

关键词： remote sensing canopy cover viticultural management frost damage digital surface model

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Development of a mobile application for identification of grapevine(Vitis vinifera L.)cultivars via deep learning

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International Journal of Agricultural and Biological Engineering 2021年第5期14卷 172-179页

作者： Yixue Liu Jinya su Lei Shen Nan Lu Yulin Fang Fei Liu Yuyang Song baofeng su College of Mechanical and Electronic Engineering Northwest A&F UniversityYangling 712100ShaanxiChina School of Computer Science and Electronic Engineering University of EssexColchesterCO43SQUK College of Water Resources and Architectural Engineering Northwest A&F UniversityYangling 712100ShaanxiChina College of Enology Northwest A&F UniversityYangling 712100ShaanxiChina College of Biosystems Engineering and Food Science Zhejiang UniversityHangzhou 310058China

Traditional vine variety identification methods usually rely on the sampling of vine leaves followed by physical,physiological,biochemical and molecular measurement,which are destructive,time-consuming,labor-intensive and require experienced grape phenotype *** mitigate these problems,this study aimed to develop an application(App)running on Android client to identify the wine grape automatically and in real-time,which can help the growers to quickly obtain the variety *** results showed that all Convolutional Neural Network(CNN)classification algorithms could achieve an accuracy of over 94%for twenty-one categories on validation data,which proves the feasibility of using transfer deep learning to identify grape species in field *** particular,the classification model with the highest average accuracy was GoogLeNet(99.91%)with a learning rate of 0.001,mini-batch size of 32,and maximum number of epochs in *** results of the App on Android devices also confirmed these results.

关键词： deep learning mobile phone grapevine cultivar vine leaf image identification Vitis vinifera L

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电解铝厂脱硫废水治理技术应用实践

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山西冶金 2024年第4期47卷 198-199,202页

作者：苏宝峰李成元王岩李文超王世杰山东南山铝业股份有限公司山东龙口265700

阳极是电解铝生产过程中一项必不可少的原材料,在参加电化学反应过程中,阳极中的硫会发生氧化,形成二氧化硫气体随着烟气进入净化系统,电解铝厂大都采用石灰石-石膏湿法脱硫技术进行处理,但在处理的过程中又会产生脱硫废水,影响环境。... 详细信息

阳极是电解铝生产过程中一项必不可少的原材料,在参加电化学反应过程中,阳极中的硫会发生氧化,形成二氧化硫气体随着烟气进入净化系统,电解铝厂大都采用石灰石-石膏湿法脱硫技术进行处理,但在处理的过程中又会产生脱硫废水,影响环境。针对该问题,对脱硫废水各种治理方法进行简介,并将物化法处理脱硫废水工艺应用于山东某电解铝公司,通过对脱硫前后水质化验结果以及脱硫系统使用前后的用水量进行统计,验证了治理措施的有效性,为电解铝行业处理脱硫废水提供了一种较好的方法。

关键词：脱硫废水处理电解铝环境

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