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Neural Regeneration Research 2018年第2期13卷 265-271页

作者： Chun-mei Wang Xue-lu Yang Ming-hui Liu bao-hua cheng Jing Chen Bo Bai Neurobiology Institute Jining Medical University

miRNAs regulate a variety of biological processes through pairing-based regulation of gene expression at the 3＇ end of the noncoding region of the target miRNA, miRNAs were found to be abnormally expressed in ischemia/reperfusion injury models. High-throughput sequencing is a recently developed method for sequencing miRNAs and has been widely used in the analysis of miRNAs. In this study, ischemia/reperfusion injury models were intracerebroventricularly injected with 50 pg/kg apelin-13. High-throughput sequencing showed that 357 known miRNAs were differentially expressed among rat models, among which 78 changed to 〉 2-fold or 〈 0.5-fold. Quantita- tive real-time polymerase chain reaction was selected to confirm the expression levels of four miRNAs that were differentially expressed, the results of which were consistent with the results of high-throughput sequencing. Gene Ontology analysis revealed that the predicted targets of the different miRNAs are particularly associated with cellular process, metabolic process, single-organism process, cell, and binding. Kyoto Encyclopedia of Gene and Genome analysis showed that the target genes are involved in metabolic pathways, mitogen-ac- tivated protein kinase signaling pathway, calcium signaling pathway, and nuclear factor-KB signaling pathway. Our findings suggest that differentially expressed miRNAs and their target genes play an important role in ischemia/reperfusion injury and neuroprotection by apelin-13.

关键词： nerve regeneration RNA sequencing microRNA apelin ischemia/reperfusion injury neuroprotection neuropeptide high-throughput sequencing neural regeneration

Lateral intracerebroventricular injection of Apelin-13 inhibits apoptosis after cerebral ischemia/reperfusion injury

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Neural Regeneration Research 2015年第5期10卷 766-771页

作者： Xiao-ge Yan bao-hua cheng Xin Wang Liang-cai Ding Hai-qing Liu Jing Chen Bo Bai Xinxiang Medical University Neurobiology Institute Jining Medical University Taishan Medical University

Apelin- 13 inhibits neuronal apoptosis caused by hydrogen peroxide, yet apoptosis following cerebral ischemia-reperfusion injury has rarely been studied. In this study, Apelin-13 （0.1 μg/g） was injected into the lateral ventricle of middle cerebral artery occlusion model rats. TTC, TUNEL, and immuno- histochemical staining showed that compared with the cerebral ischemia/reperfusion group, infarct volume and apoptotic cell number at the ischemic penumbra region were decreased in the Apelin-13 treatment group. Additionally, Apelin-13 treatment increased Bcl-2 immtmoreactivity and decreased caspase-3 immunoreactivity, Our findings suggest that Apelin-13 is neuroprotective against cerebral ischemia/reperfusion injury through inhibition of neuronal apoptosis.

关键词： nerve regeneration brain injury neuroprotection cerebral ischemia/reperfusion injury lateral intracerebroventricular injection Apelin-13 nerve apoptosis Bcl-2 caspase-3 NSFC grants neural regeneration

Apelin-13 inhibits apoptosis and excessive autophagy in cerebral ischemia/reperfusion injury

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Neural Regeneration Research 2021年第6期16卷 1044-1051页

作者： Zi-Qi Shao Shan-Shan Dou Jun-Ge Zhu Hui-Qing Wang Chun-Mei Wang bao-hua cheng Bo Bai Cheeloo College of Medicine Shandong UniversityJinanShandong ProvinceChina Neurobiology Institute Jining Medical UniversityJiningShandong ProvinceChina

Apelin-13 is a novel endogenous ligand for an angiotensin-like orphan G-protein coupled receptor,and it may be neuroprotective against cerebral ischemia ***,the precise mechanisms of the effects of apelin-13 remain to be *** investigate the effects of apelin-13 on apoptosis and autophagy in models of cerebral ischemia/reperfusion injury,a rat model was established by middle cerebral artery ***-13(50μg/kg)was injected into the right ventricle as a *** addition,an SH-SY5Y cell model was established by oxygen-glucose deprivation/reperfusion,with cells first cultured in sugar-free medium with 95%N2 and 5%CO2 for 4 hours and then cultured in a normal environment with sugar-containing medium for 5 *** SH-SY5Y cell model was treated with 10-7 M apelin-13 for 5 *** showed that apelin-13 protected against cerebral ischemia/reperfusion ***-13 treatment alleviated neuronal apoptosis by increasing the ratio of Bcl-2/Bax and significantly decreasing cleaved caspase-3 *** addition,apelin-13 significantly inhibited excessive autophagy by regulating the expression of LC3B,p62,and ***,the expression of Bcl-2 and the phosphatidylinositol-3-kinase(PI3K)/Akt/mammalian target of rapamycin(mTOR)pathway was markedly *** LY294002(20μM)and rapamycin(500 nM),which are inhibitors of the PI3K/Akt/mTOR pathway,significantly attenuated the inhibition of autophagy and apoptosis caused by *** conclusion,the findings of the present study suggest that Bcl-2 upregulation and mTOR signaling pathway activation lead to the inhibition of apoptosis and excessive *** effects are involved in apelin-13-induced neuroprotection against cerebral ischemia/reperfusion injury,both in vivo and in *** study was approved by the Animal Ethical and Welfare Committee of Jining Medical University,China(approval No.2018-JS-001)in February 2018.

关键词： central nervous system brain brain injury factor pathways apoptosis autophagy neuroprotection regeneration

Traditional El Nio and El Ni o Modoki Revisited:Is El Nio Modoki Linearly Independent of Traditional El Nio?

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Atmospheric and Oceanic Science Letters 2010年第2期3卷 70-74页

作者： LI Gen REN bao-hua YANG cheng-Yun ZHENG Jian-Qiu School of Earth and Space Sciences University of Science and Technology of China Hefei 230026 China

The present study revisited the first two leading modes of tropical Pacific sea surface temperature anomalies （SSTA） during the period of 1979-2008. It is suggested that the so-called El Nino Modoki, which is captured by the second mode, exists objectively and exhibits obvious differences from traditional El Nifio, which is captured by the first mode, in terms of its spatial characteristics. Furthermore, the authors found that El Nino Modoki is linearly independent of traditional El Nino; hence, it cannot be described as part of the traditional El Nino evolution, and vice versa.

关键词： El Nifio El Nifio Modoki linear independence

应用于扫描探针显微镜的石英音叉机械模型研究

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物理学报 2011年第4期60卷 108-113页

作者：华宝成钱建强王曦姚骏恩北京航空航天大学仪器科学与光电工程学院北京100191 微纳测控与低维物理教育部重点实验室北京100191 北京航空航天大学应用物理系北京100191

石英音叉作为力传感器广泛地应用于各种扫描探针显微镜,主要涉及石英音叉的同相振动和反相振动两种振动模式.通过实验方法和有限元仿真方法对石英音叉的两种振动模式进行研究,发现石英音叉的双臂之间以及双臂与音叉的基部之间都存在耦... 详细信息

石英音叉作为力传感器广泛地应用于各种扫描探针显微镜,主要涉及石英音叉的同相振动和反相振动两种振动模式.通过实验方法和有限元仿真方法对石英音叉的两种振动模式进行研究,发现石英音叉的双臂之间以及双臂与音叉的基部之间都存在耦合作用,双臂之间的耦合使音叉的反相共振频率升高,双臂与基部之间的耦合使音叉的同相共振频率降低.针对两种振动模式的动态特性建立了石英音叉的机械模型并进行合理简化.简化模型是一个四弹簧三质点系统,计算了简化模型的参数.通过一个音叉臂等效质量变化与音叉反相共振频率变化之间的定量关系证明了简化机械模型能够准确地反映石英音叉应用在扫描探针显微镜中的机械特性.

关键词：扫描探针显微镜石英音叉振动模式机械模型

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天问一号着陆器双目视觉避障技术

宇航学报 2022年第1期43卷 56-63页

作者：华宝成李涛刘洋张依诺王立北京控制工程研究所北京100094

对天问一号双目视觉避障技术的组成、方案及原理进行了介绍,通过图像障碍识别和双目立体地形感知完成天问一号降落过程中的安全着陆点自动选取。通过高分辨率双目匹配和亚像素插值处理,提高远距离双目测量精度;在计算资源严格受限的条件... 详细信息

对天问一号双目视觉避障技术的组成、方案及原理进行了介绍,通过图像障碍识别和双目立体地形感知完成天问一号降落过程中的安全着陆点自动选取。通过高分辨率双目匹配和亚像素插值处理,提高远距离双目测量精度;在计算资源严格受限的条件下,采用FPGA作为硬件加速平台,通过功能模块的并行化和流水线设计,提高计算视差和三维信息的速度;为克服在轨恶劣环境导致的参数变化,设计在轨自标定方法实现双目对正参数表的自主更新;开展了地面试验和在轨应用,验证了双目视觉避障技术的有效性,实现2 K×2 K高分辨率双目稠密匹配,匹配用时1 s;三维地形测量精度达到6.4 cm@70 m,坡度识别精度0.5°;双目避障系统成功完成了在轨自标定处理,在轨图像能够有效恢复天问一号着陆点区域的三维地形。

关键词：天问一号着陆器双目视觉避障系统 FPGA 双目立体匹配

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嫦娥五号探测器自主着陆视觉避障方法与评价

宇航学报 2021年第8期42卷 975-981页

作者：王立刘洋华宝成李涛朱飞虎北京控制工程研究所北京100190

对嫦娥五号探测器的视觉自主避障系统组成、方案及原理进行介绍。采用光学粗避障加激光精避障的两级接力避障模式,在光学粗避障阶段利用K均值聚类对图像障碍进行分割,对分割结果采用Delaunay三角剖分,并设计安全系数评价方法选择安全落... 详细信息

对嫦娥五号探测器的视觉自主避障系统组成、方案及原理进行介绍。采用光学粗避障加激光精避障的两级接力避障模式,在光学粗避障阶段利用K均值聚类对图像障碍进行分割,对分割结果采用Delaunay三角剖分,并设计安全系数评价方法选择安全落点;在激光精避障阶段提出概率模型评价点云所示区域的着陆安全性。对两级避障的在轨图像利用上述方法进行仿真分析,并对着陆区域的安全性进行评价。结果显示两级避障均合理有效。

关键词：嫦娥五号探测器自主着陆视觉避障系统

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小天体在轨渲染观测技术

光学精密工程 2021年第12期29卷 2745-2753页

作者：刘兴潭张成渝武延鹏华宝成吴奋陟中国空间技术研究院北京控制工程研究所北京100190

提出了一种空间光场中小天体在轨序列图像渲染观测方法,综合小天体的表面反射特性、轨道特性与观测需求,提供目标在观测时刻的位姿序列、光照角度和相机特性等参数,生成符合探测器成像特性的物理渲染图像集。完成了三维基准模型的建立... 详细信息

提出了一种空间光场中小天体在轨序列图像渲染观测方法,综合小天体的表面反射特性、轨道特性与观测需求,提供目标在观测时刻的位姿序列、光照角度和相机特性等参数,生成符合探测器成像特性的物理渲染图像集。完成了三维基准模型的建立、反射特性文件的生成及物理渲染的光线追迹。由渲染图像真实度检验与地形三维建模实验,针对糸川小行星在特定观测时刻与位置的图像进行闭环模拟观测验证。实验结果表明,通过匹配重建模型的二维投影地形图,图像灰度值配准的平均均方根误差为0.1785,对应小天体地表0.0628 m左右的海拔高差。该渲染观测方法能够有效服务于小天体地形模型重建与相对导航闭环分析环境的搭建。此外,为满足在轨快速解算需求,提出了归一化光谱与灰度响应的快速转化方法,在精度不变的情况下,解算时间为3.48 s,由数小时缩短至秒级。

关键词：小天体空间光场物理渲染数字地形模型观测平台

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状态激励轻敲模式原子力显微镜的设计与仿真

电子显微学报 2011年第1期30卷 1-6页

作者：华宝成钱建强姚骏恩北京航空航天大学仪器科学与光电工程学院北京100191 北京航空航天大学微纳测控与低维物理教育部重点实验室北京100191 北京航空航天大学物理科学与核能工程学院北京100191

为轻敲模式原子力显微镜设计了一种激励方式,在带有状态反馈的轻敲模式原子力显微镜基础上,除去原有的外加激励信号而引入一个自动增益控制环节,用于维持状态反馈信号的幅度恒定,并将此信号作为悬臂振动的激励信号。该激励方式即为状态... 详细信息

为轻敲模式原子力显微镜设计了一种激励方式,在带有状态反馈的轻敲模式原子力显微镜基础上,除去原有的外加激励信号而引入一个自动增益控制环节,用于维持状态反馈信号的幅度恒定,并将此信号作为悬臂振动的激励信号。该激励方式即为状态激励,用于轻敲模式时可称为状态激励轻敲模式原子力显微镜。采用Matlab/Simulink工具实现了对状态激励轻敲模式原子力显微镜系统的建模和数值仿真,起振曲线证明了状态激励信号能够驱动悬臂振动并调整其工作频率;逼近曲线、线扫描曲线等典型功能仿真实验证明了状态激励方式能够成功地应用于轻敲模式成像。

关键词：原子力显微镜轻敲模式状态激励数值仿真

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某牵引车多体系统动力学建模与仿真

上海交通大学学报 2003年第11期37卷 1767-1771页

作者：包继华孙承顺张建武张鑫上海交通大学机械与动力工程学院上海200030

利用多体理论对某牵引车建立了整车非线性系统动力学模型,模型中将钢板弹簧离散为多个无质量Timoshenko梁连接的柔性体,以模拟钢板弹簧的非线性特性.将整车模型在方向盘正弦输入下进行仿真的结果表明,车辆的操纵性能与方向盘输入有较好... 详细信息

利用多体理论对某牵引车建立了整车非线性系统动力学模型,模型中将钢板弹簧离散为多个无质量Timoshenko梁连接的柔性体,以模拟钢板弹簧的非线性特性.将整车模型在方向盘正弦输入下进行仿真的结果表明,车辆的操纵性能与方向盘输入有较好的跟随性,但由于非线性特性的影响而存在滞后现象,同时载荷转移使得车辆转弯时内侧的侧向力比外侧的侧向力小,且变化相对平缓.该模型较好地反映了车辆实际运行的动力学特性,其建模方法可以应用于其他计及非线性效应车辆的动力学响应的研究。

关键词：多体理论钢板弹簧建模仿真