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World Journal of Gastroenterology 2008年第18期14卷 2894-2899页

作者： Li Li Xin-Yan Zhao bao-en wang Liver Research Center Beijing Friendship Hospital Capital Medical University Beijing 100050 China

AIM: To investigate the effect of herbal compound 861 (Cpd861) on the transforming growth factor-β1 (TGFβ1)/ activin receptor-like kinase 1 (ALK1, type Ⅰ receptor) signaling-pathway-related gene expression in the LX-2 cell line, and the inhibitory mechanism of Cpd861 on the activation of LX-2 cells. METHODS: LX-2 cells were treated with TGFβ1 (5 ng/mL) Cpd861 (0.1 mg/mL), TGFβ1 (5 ng/mL) plus Cpd861 (5 ng/mL) for 24 h to investigate the effect of Cpd861 on the TGFβ1/ALK1 pathway. Real-time PCR was performed to examine the expression of α-SMA (α-smooth muscle actin), ALK1, Id1 (inhibitor of differentiation 1). Western blotting was carried out to measure the levels of α-SMA and phosphorylated Smad1, and immunocytochemical analysis for the expression of α-SMA. RESULTS: In LX-2 cells, TGFβ1/ALK1-pathway-related gene expression could be stimulated by TGFβ1, which led to excessive activation of the cells. Cpd861 decreased the activation of LX-2 cells by reducing the expression of α-SMA mRNA and protein expression. This effect was related to inhibition of the above TGFβ1/ALK1-pathway- related expression of genes such as Id1 and ALK1, and phosphorylation of Smad1 in LX-2 cells, even with TGFβ1 co-treatment for 24 h. CONCLUSION: Cpd861 can restrain the activation of LX-2 cells by inhibiting the TGFβ1/ALK1/Smad1 pathway.

关键词： Herbal compound 861 LX-2 cell Activin receptor-like kinase 1 Inhibitor of differentiation 1 Smad1

Herbal compound 861 regulates mRNA expression of collagen synthesis- and degradation-related genes in human hepatic stellate cells

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World Journal of Gastroenterology 2008年第11期14卷 1790-1794页

作者： Lin wang Jlan wang Xue-Hai Tan bao-en wang Pei-Gen Xiao Beijing Genomic Institute Beijing 101300 China School of Chinese Materia Medica Beijing Universityof Chinese Medicine Beijing 101300 China Liver Research Center Beijing Friendship Hospital Capital University of Medical Sciences Beijing 101300 China Institute of Medicinal Plant Development Chinese Academy of Medical Sciences Beijing 101300 China

AIM： To identify the role of herbal compound 861 （Cpd 861） in the regulation of mRNA expression of collagen synthesis- and degradation-related genes in human hepatic stellate cells （HSCs）. METHODS： mRNA levels of collagen types I and III, matrix metalloproteinase 1 （MMP-1）, matrix metalloproteinase 2 （MMP-2）, membrane type-1 matrix metalloproteinase （MT1-MMP）, tissue inhibitor of metalloproteinase 1 （TIMP-1）, and transforming growth factor β1 （TGF-β1） in cultured-activated HSCs treated with Cpd 861 or interferon-γ, （IFN-γ,） were determined by real-time PCR. RESULTS： Both Cpd 861 and IFN-γ reduced the mRNA levels of collagen type Ⅲ, MMP-2 and TGF-β1. Moreover, Cpd 861 significantly enhanced the MMP-1 mRNA levels while down-regulated the TIMP-1 mRNA expression, increasing the ratio of MMP-1 to TIMP-1 to （6.3 ＋ 0.3）- fold compared to the control group. CONCLUSION： The anti-fibrosis function of Cpd 861 may be mediated by both decreased interstitial collagen sythesis by inhibiting the transcription of collagen type Ⅲ and TGF-β1 and increased degradation of these collagens by up-regulating MMP-1 and down-regulating TIMP-1 mRNA levels.

关键词： Herbal Compound 861 Human hepatic stellate cells Collagen synthesis and degration Collagen type Ⅲ Matrix metalloproteinase 1 Tissue inhibitor of metalloproteinase 1

Activation of canonical Wnt signaling pathway promotes proliferation and self-renewal of rat hepatic oval cell line WB-F344 in vitro

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World Journal of Gastroenterology 2008年第43期14卷 6673-6680页

作者： Ying Zhang Xin-Min Li Fu-Kui Zhang bao-en wang Liver Research Center Beijing Friendship Hospital Capital Medical University Beijing 100050 China

AIM： To investigate the effect of activation of canonical Wnt signaling pathway on the proliferation and differentiation of hepatic oval cells in vitro. METHODS： WB-F344 cells were treated with recombinant Wnt3a （20, 40, 80, 160, 200 ng/mL） in serum-free medium for 24 h. Cell proliferation was measured by Brdu incorporation analysis; untreated WB-F344 cells were taken as controls. After treatment with Wnt3a （160 ng/mL） for 24 h, subcellular localization and protein expression of p-catenin in WB-F344 cells treated and untreated with Wnt3a were examined by immunofluorescence staining and Western blot analysis. CyclinD1 mRNA expression was determined by semi-quantitative reverse-transcript polymerase chain reaction （RT-PCR）. The mRNA levels of some phenotypic markers （AFP, CK-19, ALB） and two hepatic nuclear factors （HNF-4, HIVF-6） were measured by RT-PCR. Expressions of CK-19 and AFP protein were detected by Western blot analysis. RESULTS： Wnt3a promoted proliferation of WB-F344 cells. Stimulation of WB-F344 cells with recombinant Wnt3a resulte^l in accumulation of the transcriptional activator β-catenin, together with its translocation into the nuclei, and up-regulated typical Wnt target gene CyclinD1. After 3 d of Wnt3a treatment in the absence of serum, WB-F344 cells retained their bipotential to express several specific phenotypic markers of hepatocytes and cholangiocytes, such as AFP and CK-19, following activation of the canonical Wnt signaling pathway. CONCLUSION： The canonical Wnt signaling pathway promotes proliferation and self-renewal of rat hepatic oval cells.

关键词： Canonical Wnt signaling pathway Oval cells Cell proliferation Self-renewal of cells

Undifferentiated connective tissue diseases-related hepatic injury

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World Journal of Gastroenterology 2008年第17期14卷 2780-2782页

作者： Ying Zhang Fu-Kui Zhang Xiao-Ning Wu Tai-Ling wang Ji-Dong Jia bao-en wang Liver research center Beijing Friendship HospitalAffiliated to Capital Medical University Beijing 100050 China Department of Pathology China-Japan Friendship Hospital Beijing 100050 China

Hepatic injury is rarely associated with undifferentiated connective tissue diseases (UCTD). We report, here, a case of a middle-aged woman with UCTD-related hepatic injury, including its case history, clinical manifestations, laboratory findings, treatment and its short-term effect. The patient was admitted to the hospital with symptoms of fatigue, anorexia, low-grade fever and skin rashes. She had a past history of left knee joint replacement. Laboratory tests showed elevated levels of serum transaminase, IgG and globulin, accelerated erythrocyte sedimentation rate, eosinophilia and a high titer of antinuclear antibodies (1:320). Imaging studies showed interstitial pneumonitis and hydropericardium. Liver biopsy showed the features which were consistent with those of connective tissue diseases-related polyangitis. After treatment with a low-dose of oral prednisone, both symptoms and laboratory findings were significantly improved. UCTD-related hepatic injury should be considered in the differential diagnosis of connective tissue diseases with abnormal liver function tests. Low- dose prednisone may effectively improve both symptoms and laboratory tests.

关键词： Undifferentiated connective tissue diseases Hepatic injury Glucocorticoid

Changes in intestinal mucosal immune barrier in rats with endotoxemia

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World Journal of Gastroenterology 2009年第46期15卷 5843-5850页

作者： Chong Liu Ang Li Yi-Bing Weng Mei-Li Duan bao-en wang Shu-Wen Zhang Departmentof Critical Care Medicine Beijing Friendship Hospital Affiliatedto Capital Medical University Beijing 100050 China Liver Research Center Beijing FriendshipHospital Affiliated to Capital Medical University Beijing100050 China Department of Infectious Disease andCritical Care Medicine Beijing Friendship Hospital Affiliatedto Capital Medical University Beijing 100050 China

AIM： To investigate the dysfunction of the immunological barrier of the intestinal mucosa during endotoxemia and to elucidate the potential mechanism of this dysfunction. METHODS： Male Wistar rats were randomly distributed into two groups： control group and lipopolysaccharide （LPS） group. endotoxemia was induced by a single caudal venous injection of LPS. Animals were sacrificed in batches 2, 6, 12 and 24 h after LPS infusion. The number of microfold （M）-cells, dendritic cells （DCs）, CD4＋ T cells, CD8＋ T cells, regulatory T （Tr） cells and IgA＋ B cells in the intestinal mucosa were counted after immunohistochemical staining. Apoptotic lymphocytes were counted after TUNEL staining. The levels of interleukin （IL）-4, interferon （IFN）-γ, and forkhead box P3 （Foxp3） in mucosal homogenates were measured by ELISA. The secretory IgA （sIgA） content in the total protein of one milligram of small intestinal mucus was detected using a radioimmunological ***： This research demonstrated that LPS-induced endotoxemia results in small intestinal mucosa injury. The number of M-cells, DCs, CD8~ T cells, and IgA~ B cells were decreased while Tr cell and apoptotic lymphocyte numbers were increased significantly. The number of CD4＋ T cells increased in the early stages and then slightly decreased by 24 h. The level of IL-4 significantly increased in the early stages and then reversed by the end of the study period. The level of IFN-T increased slightly in the early stages and then decreased markedly by the 24 h time point. Level of Foxp3 increased whereas sIgA level ***： Mucosal immune dysfunction forms part of the intestinal barrier injury during endotoxemia. The increased number and function of Tr cells as well as lymphocyte apoptosis result in mucosal immunode- ficiency.

关键词： endotoxemia Rats Intestinal mucosa Immunity

Grain size characteristics and genesis of the Muxing loess in the Muling- Xingkai Plain,Northeast China

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Journal of Groundwater Science and engineering 2021年第2期9卷 152-160页

作者： Zhong-shuang Cheng Chen Su Zhao-xian Zheng Zhuang Li Li-kang wang en-bao wang Institute of Hydrogeology and environmental Geology Chinese Academy of Geological ScienceShijiazhuang 050061China Key Laboratory of Groundwater Science and engineering Ministry of Natural ResourceShijiazhuang 050061China Shandong Geological environment Monitoring Station Jinan 250014China Research of Regional Geological Survey of Heilongjiang Harbin 150036China

Thick loess is deposited on the platform in the piedmont zone of Muling-Xingkai Plain(Muxing Plain),but the genesis of the Muxing loess is still *** aims of this study are to analyze the grain size characteristics of Muxing loess collected from the cores of a typical borehole(ZK1)in the piedmont zone of Muxing Plain,and to verify its *** Muxing loess is mainly composed of the particles with diameter less than 50μm,with an average content of 92.48%.The coarse silt particles with diameter of 10-50μm are the basic composition of aeolian sediments,and their average content is 44.34% for the Muxing loess,which is the mode class among the particles with different *** grain size parameters and frequency curves are similar to those of the typical aeolian *** distribution characteristic of the Muxing loess in the C-M scatter diagram is consistent with that of the Xi Feng *** addition,the discriminant analysis shows the Muxing loess mostly consists of aeolian ***,it can be concluded that the Muxing loess mainly resulted from aeolian deposition based on the grain size *** Plain is dominated by the monsoon climate,and the wind-blown dusts are gradually deposited after being transported over long distances.

关键词： Muxing Plain Loess Grain size Characteristics Aeolian deposition

Establishment and Application of Early Risk Stratification Method for Acute Abdominal Pain in Adults

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Chinese Medical Journal 2017年第5期130卷 530-535页

作者： Yu wang Hong Zhao Zhen Zhou Ci Tian Hong-Li Xiao bao-en wang Department of Emergency Medicine Beijing Tongren Hospital Capital Medical University Beijing 100730 China Department of Biomedical Informatics School of Biomedical Engineering Capital Medical University Beijing 100069 China Department of Emergency Medicine Peking University Third Hospital Capital Medical University Beijing 100191 China Department of Emergency Medicine Beijing Friendship Hospital Capital Medical University Beijing 100050 China Department of Gastroenterology Beijing Friendship Hospital Capital Medical University Beijing 100050 China

Background： Acute abdominal pain is a common symptom of emergency patients. The severity was always evaluated based on physicians＇ clinical experience. The aim of this study was to establish an early risk stratification method （ERSM） for addressing adults with acute abdominal pain, which would gnide physicians to take appropriate and timely measures following the established health-care policies. Methods： In Cohort 1, the records of 490 patients with acute abdominal pain that developed within the past 72 h were enrolled in this study. Measurement data and numeration data were compared with analysis of variance and Chi-square test, respectively. Multiple regression analysis calculated odd ratio （OR） value. P and OR values showed the impacts of factors. ERSM was established by clinical experts and statistical experts according to Youden index. In Cohort 2, data from 305 patients with acute abdominal pain were enrolled to validate the accuracy of the ERSM. Then, ERSM was prospectively used in clinical practice. Results： The ERSM was established based on the scores of the patient＇s clinical characteristics： right lower abdominal pain ＋ 3 × diffuse abdominal pain ＋ 3 ×cutting abdominal pain ＋ 3 × pain frequency ＋ 3 × pain duration ＋ fever ＋ 2 × vomiting ＋ 5 × stop defecation ＋ 3 - history of abdominal surgery ＋ hypertension history ＋ diabetes history ＋ hyperlipidemia history ＋ pulse ＋ 2 - skin yellowing ＋ 2 × sclera yellowing ＋ 2 × double lung rale ＋ 10 × unconsciousness ＋ 2 ×right lower abdominal tenderness ＋ 5 x diffuse abdominal tenderness ＋ 4 x peritoneal irritation ＋ 4 × bowel sounds abnormal ＋ 10 × suspicious diagnosis ＋ white blood cell count ＋ hematocrit ＋ glucose ＋ 2 × blood urea nitrogen ＋ 3 × creatine ＋ 4× serum albumin ＋ alanine aminotransferase ＋ total bilirubin ＋ 3 × conjugated bilirubin ＋ amylase. When the score was 〈18, the patient did not need hospitalization. A score of≥18 and 〈38 indicated that the patient should be under observation or hospitalized. A

关键词： Acute Abdominal Pain Emergency Risk Factor Stratification

Practice of improving the hot blast temperature for the 2500 m^3 BF

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baosteel Technical Research 2008年第2期2卷 31-35页

作者： Li Yiwei Shi Jian＇ en bao Lei wang Yi Ironmaking Plant Stainless Steel Branch Baoshan Iron ＆ Steel Co. Ltd. Shanghai 200431 China

A high blast temperature is an effective measure that improves pulverized coal injection （PCI）and energy consumption,decreases the cost of blast furnaces （BF）. Apart from using technology such as the increased vault temperature of the hot-blast stove, the increased temperature of the waste gas, the preheating of combustion air and gas, and fully closed mixed air valves, other measures, such as using an oxygen-enriched blast stove, burning high heating value converter gas, increasing the number of stove changes to reduce the time needed for stove blasts, were taken to increase the blast temperature of the 2 500 m3 BF in recent years. The blast temperature of the 2 500 m3 BF was continuously increased in these years. The monthly average temperature of the 2 500 m3 BF reached 1 158℃, with the highest blast temperature reaching 1 195 ℃ in 2006. This technique of increased blast temperatures has reached an advanced level in China.

关键词： hot blast stove high blast temperature oxygen-enriched stove converter gas

Clinical evaluation of serum antimitochondrial antibody-negative primary biliary cirrhosis

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Hepatobiliary & Pancreatic Diseases International 2004年第2期3卷 288-291页

作者： Fu-Kui Zhang, Ji-Dong Jia and bao-en wang Beijing, China Liver Research Center, Beijing Friendship Hospital, Capital University of Medical Sciences, Beijing 100050 , China Liver Research Center Beijing Friendship Hospital Capital University of Medical Sciences Beijing 100050 China. frankliver@***

BACKGROUND: Primary biliary cirrhosis (PBC) is cha- racterized by frequent presence of antimitochondrial anti- bodies (AMAs). The sensitivity and specificity of AMA for PBC are both greater than 90%-95%, so the presence of AMA in serum is the major hallmark in PBC. However, it has long been recognized that in 5%-10% of patients the clinical, biochemical and histological features are diagnos- tic for PBC, but their sera are consistently tested negative for AMA/AMA-M2. This study aimed to evaluate whether the presence of AMA alters the clinical, serological and his- tological features of the disease. METHODS: Clinical data of 70 patients clinically and/or histologically diagnosed with PBC were reviewed. AMA- negative and AMA-positive patients were compared in terms of clinical, biochemical, immunological and histo- logical features. RESULTS: At presentation, 11 patients were serum AMA/ AMA-M2 negative. At the initial visit, AMA-negative and AMA-positive patients were similar in terms of age, sex, clinical manifestations, liver biochemistries and histological findings. The mean level of serum immunoglobulin M (IgM) was significantly lower in AMA-negative PBC pa- tients than in AMA-positive PBC patients (2851±1418 mg/L vs 6361 ±4928 mg/L, P =0.033). Serum antinuclear anti- bodies ( ANA) and/or smooth muscle antibodies ( SMA) were more frequently positive in the AMA-negative PBC patients than in the AMA-positive patients (81.8% vs 40.7%, P =0.031). CONCLUSION: AMA-negative PBC patients are characte- rized by relatively lower levels of serum IgM and a higher prevalence of serum ANA/SMA and are not associated with substantial differences in the clinical, biochemical and histo- logical spectrum of the disease.

关键词： primary biliary cirrhosis antimitochondrial antibody antinuclear antibody immunoglobulin M

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肝星状细胞中的TGFβ信号转导通路

世界华人消化杂志 2007年第23期15卷 2516-2520页

作者：李丽王宝恩首都医科大学附属北京友谊医院肝病中心北京市100050

肝星状细胞(hepatic stellate cell,HSC)活化是肝纤维化形成的中心环节,转化生长因子β(transforming growth factorβ,TGFβ)启动了肝星状细胞的活化,TGFβ信号在HSC内主要通过ALK5/smad2/3途径在HSC内传递,而近年来的研究表明,ALK1/smad 1/5途径及非smad信号也起非常重要的作用.有必要对HSC中复杂的TGFβ信号进行研究,为有效抗TGFβ治疗肝纤维化提供依据.

关键词：肝星状细胞转化生长因子β 信号转导通路