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Effect of NS398 on metastasis-associated gene expression in a human colon cancer cell line

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World Journal of Gastroenterology 2005年第28期11卷 4337-4343页

作者： Xue-Qin Gao Jin-Xiang Han Hai-Yan Huang bao song, Bo Zhu Chang-Zheng song Key Laboratory of Ministry of Public Health for Biotech-Drug Shandong Medicinal and Biotechnology Center Shandong Academy of Medical Sciences Jinan 250062Shandong Province China Shandong University School of Medicine Jinan 250062 Shandong Province China

AIM： To investigate the effect of NS398 on the metastasis-associated gene expression in LoVo colorectal cancer cells.METHODS： LoVo cells were treated with NS398 at the concentration of 100 IJmol/L for 24 and 48 h respectively. Total RNA was extracted with TRIzol reagents and reverse transcribed with Superscript Ⅱ and hybridized with cDNA mlcroarray （containing oncogenes, tumor suppressor genes, signal transduction molecules, adhesive molecules, growth factors, and ESTs） fabricated in our laboratory. After normalization, the ratio of gene expression of NS398 treated to untreated LoVo cells was either 2-fold up or 0.5-fold down was defined as the differentially expressed genes. Semi-quantitative RT-PCR was used to validate the microarray results.RESULTS： Among the 447 metastasis-associated genes, 9 genes were upregulated and 8 genes were downregulated in LoVo cells treated with NS398 for 24 h compared to untreated cells. While 31 genes were upregulated and 14 genes were downregulated in LoVo cells treated with NS398 for 48 h. IGFBP-5, PAI-2, JUN,REL, BRCA1, and BRCA2 might be the new targets of NS398 in treatment of colorectal cancer.CONCLUSION： NS398 might exert its anti-metastasis effect on colorectal cancer by affecting several metastasis-associated gene expression.

关键词： NS398 Colorectal cancer gene expression Metastasis cDNA microarray

“一带一路”视域下语言测试对高校英语教学的反拨效用研究

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黑龙江教师发展学院学报 2024年第8期43卷 68-71页

作者：岳欣杨传鸣宋宝梅东北农业大学哈尔滨150030

“一带一路”视域下的复合型人才培养,已上升到国家战略层面。语言测试作为考查、评估和改进语言教学的重要手段,有助于学生了解学习成效;教师明确教学状态,优化教学行为,提高教学效果。基于此,分析语言测试的意义,探究国内外反拨效用... 详细信息

“一带一路”视域下的复合型人才培养,已上升到国家战略层面。语言测试作为考查、评估和改进语言教学的重要手段,有助于学生了解学习成效;教师明确教学状态,优化教学行为,提高教学效果。基于此,分析语言测试的意义,探究国内外反拨效用研究现状,阐述语言测试对英语教学的反拨作用,提出应用反拨效用的策略。

关键词： “一带一路” 反拨效用语言测试大学英语

融合实体信息和时序特征的问答式事件检测方法

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计算机工程与设计 2024年第4期45卷 1218-1224页

作者：马宇航宋宝燕丁琳琳鲁闻一纪婉婷辽宁大学信息学院辽宁沈阳110036

针对现有问答方法在处理触发词歧义性问题上的不足,提出一种融合实体信息和时序特征的问答式事件检测方法EDQA-EITF。构建一种基于RoBERTa的问答框架,增强模型的语义表示能力;通过在模型输入序列中显示地添加实体、实体类型等先验信息,... 详细信息

针对现有问答方法在处理触发词歧义性问题上的不足,提出一种融合实体信息和时序特征的问答式事件检测方法EDQA-EITF。构建一种基于RoBERTa的问答框架,增强模型的语义表示能力;通过在模型输入序列中显示地添加实体、实体类型等先验信息,进一步帮助模型根据句子的上下文语境对触发词进行分类;采用最小门控循环单元(minimal gated unit,MGU)和Transformer编码器对输入序列中的时序依赖关系进行建模,提升模型对于句子的语义关系、句法结构的阅读与理解能力。公共数据集上的实验结果表明,所提方法在进行事件检测时具有更优的性能,有效缓解了触发词的歧义性问题。

关键词：事件检测问答 RoBERTa 时序特征先验信息最小门控单元 Transformer

产后压力性尿失禁患者应用盆底康复治疗仪联合盆底肌训练的疗效观察

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中国现代药物应用 2024年第16期18卷 170-173页

作者：刘芳银宋宝琴王泽青南平市人民医院 353000

目的观察盆底康复治疗仪联合盆底肌训练对产后压力性尿失禁患者的临床疗效。方法选取80例年龄>20岁的产后压力性尿失禁患者为调查对象,以随机数字表法分成观察组及对照组,每组40例。对照组患者从产后42 d恶露排净后进行盆底肌训练指导,... 详细信息

目的观察盆底康复治疗仪联合盆底肌训练对产后压力性尿失禁患者的临床疗效。方法选取80例年龄>20岁的产后压力性尿失禁患者为调查对象,以随机数字表法分成观察组及对照组,每组40例。对照组患者从产后42 d恶露排净后进行盆底肌训练指导,观察组在对照组基础上联合盆底康复治疗仪治疗。比较两组患者治疗前后的盆底肌张力指标(盆底肌静息压、盆底肌收缩压、盆底肌力收缩持续时间)、漏尿量以及治疗效果。结果治疗后,两组患者盆底肌静息压、盆底肌收缩压、盆底肌力收缩持续时间均较治疗前改善,且观察组盆底肌静息压(44.45±5.11)mm Hg(1 mm Hg=0.133 kPa)、盆底肌收缩压(47.07±3.01)mm Hg、盆底肌力收缩持续时间(3.31±0.63)s优于对照组的(40.75±4.22)mm Hg、(41.16±2.02)mm Hg、(2.72±0.54)s(P<0.05)。两组患者漏尿量均较治疗前改善,且观察组患者漏尿量(2.11±0.13)g优于对照组的(3.48±0.25)g(P<0.05)。观察组患者治疗总有效率97.50%高于对照组的80.00%(P<0.05)。结论盆底康复治疗仪联合盆底肌训练,可改善产后压力性尿失禁患者盆底肌力指标,减少漏尿量,可作为非手术治疗产后压力性尿失禁的首选治疗方案。

关键词：产后压力性尿失禁盆底康复治疗仪盆底肌训练疗效

Synthesis,Characterization and Antibacterial Activity of New 5-Aryl Pyrazole Oxime Ester Derivatives

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Chemical Research in Chinese Universities 2008年第4期24卷 454-458页

作者： LIU Xin-hua ZHI Li-ping song bao-an XU Hong-li Key Laboratory of Anhui Educational Department Faculty of Mathematics ＆ Physics Anhui University of Technology Ma ＇anshan 243002 P R. China Key Laboratory of Green Pesticide and Agriculture Bioengineering Ministry of Education Guizhou University Guiyang 550025 P. R. China

Eleven new 1-{5-[4-（benzyloxy）phenyl]-3-methyl-4,5-dihydropyrazol-l-yl} oxime ester dcrivatives were synthesized and characterized by elemental analysis, HRMS, ^1H NMR data. All the compounds were screened for their antibacterial potential in vitro against Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. The results indicate that compounds 8c and 8f possess potent activity with the minimum inhibitory concentrations（MIC） of 1.562--3.125 ug/mL against all the four bacteria. Compounds 8c, 8e and 8f show moderate inhibition against the DNA gyrase（IC50=1.9--2.5 ug/mL）. On the basis of the biological activities, structure-activity relationship was discussed.

关键词： 5-Aryl pyrazole Oxime ester Antibacterial activity

Correlation between mitochondrial TRAP-1 expression and lymph node metastasis in colorectal cancer

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World Journal of Gastroenterology 2012年第41期18卷 5965-5971页

作者： Jing-Yan Gao bao-Rong song Jun-Jie Peng Yuan-Ming Lu Department of Colorectal Cancer Fudan University ShanghaiCancer Center Shanghai Medical College Fudan UniversityShanghai 200032 China Department of Human Anatomy and Histo-Em-bryology Shanghai Medical College Fudan University Shanghai200032 China Department of Oncology Shanghai MedicalCollege Fudan University Shanghai 200032 China

AIM： To evaluate the effect of mitochondrial tumor ne- crosis factor receptor-associated protein-1 （TRAP-l） on the lymph node metastasis （LNM） in Chinese colorectal cancer （CRC） patients, and develop potential LNM- associated biomarkers for CRC using quantitative real- time polymerase chain reaction （RT-PCR） analysis. METHODS： Differences in mitochondrial TRAP-1 gene expression between primary CRC with LNM （LNM CRC） and without LNM （non-LNM CRC） were assessed in 96 Chinese colorectal carcinoma samples using quantita- tive RT-PCR analysis, Western blotting, and confirmed with immunohistochemical assay. The relationship between clinicopathological parameters and potential diaclnostic biomarkers was also ***： TRAP-1 was significantly upregulated in LNM CRC compared with non-LNM CRC, which was confirmed by RT-PCR, Western blotting and immuno- histochemical assay. The expression of TRAP-1 in two different metastatic potential human colorectal cancer cell lines, LoVo and HT29, was analyzed with Western blotting. The expression level of TRAP-1 was dramati- cally higher in LoVo than in HT29. Overexpression of TRAP-1 was significantly associated with LNM （90.2% in LNM group vs 22% in non-LNM group, P 〈 0.001）, the advanced tumor node metastasis stage （89.1% in LNM group vs 26.9% in non-LNM group, P 〈 0.001）, the increased 5-year recurrence rate （82.7% in LNM group vs 22.6% in non-LNM group, P 〈 0.001） and the decreased 5-year overall survival rate （48.4% in LNM vs 83.2% in non-LNM group, P 〈 0.001）. Univariate and multivariate analyses indicated that TRAP-1 expression was an independent prognostic factor for recurrence and survival of CRC patients （Hazard ratio of 2.445 in recurrence, P = 0.017; 2.867 in survival, P = 0.028）. CONCLUSION： Mitochondria TRAP-1 affects the lymph node metastasis in CRC, and may be a potential bio- marker for LNM and a prognostic factor in CRC. Over- expression of TRAP-1 is a predictive factor for the poor outcome of colorectal cancer

关键词： Colorectal cancer Lymph node metastasis Prognosis Quantitative real-time polymerase chain reaction analysis Hsp90 family Mitochondria tumor necrosis factor receptor-associated protein-1

Association Mapping and Marker Development of Genes forStarch Lysophospholipid Synthesis in Rice

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Rice science 2016年第6期23卷 287-296页

作者： TONG Chuan LIU Lei Daniel L.E.WATERS bao Jin-song Institute of Nuclear Agricultural Sciences College of Agriculture and BiotechnologyZhejiang UniversityHangzhou 310029China Institute of Food Science Zhejiang Academy of Agricultural SciencesHangzhou 310021China Southern Cross Plant Science Southern Cross UniversityLismoreNSW 2480Australia

Phospholipids are a major kind of lipids in rice grains and have fundamental nutritional andfunctional benefits to the plant. Their lyso forms （lysophospholipids, LPLs） often form inclusion complexeswith amylose or independently influence the physicochemical and functional properties of rice ***, the genetic basis for LPL synthesis in rice endosperm is largely unknown. Here, we performeda preliminary association test of 13 LPL compositions among 20 rice accessions, and identified 22putative main-effect quantitative trait loci responsible for all LPLs except for LPC14：0 and LPE14：0. Fivederived cleaved amplified polymorphic sequences and one insertion/deletion marker for threeLPL-synthesis-related candidate genes were developed. Association analysis revealed two markerssignificantly associated with starch LPL traits. These results provide an insight into the genetic basis ofphospholipid biosynthesis in rice and may contribute to the rice quality breeding programs usingfunctional markers.

关键词： rice starch lysophospholipid phospholipid biosynthesis grain quality QTL molecularmarker association mapping

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运动学误差模型变体下的机械臂标定精度研究

机床与液压 2024年第5期52卷 37-44页

作者：蒋周翔苏瑞秦鹏举蒙月晨龙忠杰宋宝唐小琦北京信息科技大学机电工程学院北京100192 华中科技大学机械科学与工程学院湖北武汉430074

为进一步提高机械臂运动学标定精度,研究不同D-H建模方式下运动学误差辨识精度的差异,为运动学误差准确建模提供理论依据。围绕SD-H和MD-H运动学误差模型的各种变体形式,量化对比各模型变体的性态水平和线性化误差,并分析二者对运动学... 详细信息

为进一步提高机械臂运动学标定精度,研究不同D-H建模方式下运动学误差辨识精度的差异,为运动学误差准确建模提供理论依据。围绕SD-H和MD-H运动学误差模型的各种变体形式,量化对比各模型变体的性态水平和线性化误差,并分析二者对运动学标定精度的综合影响。研究结果表明:x-z-y欧拉变换会破坏MD-H运动学误差模型最小性,不适用于运动学标定;其余满秩MD-H模型性态水平优于SD-H模型两个数量级;MD-H建模时引入的线性化误差与SD-H数量级等同;在模型性态水平和线性化误差综合作用下,较强测量噪声环境中的SD-H模型运动学标定精度(0.1%~39.3%)优于MD-H模型(0.1%~71.2%);角度类与位置类的运动学参数误差的辨识精度差异显著。研究成果为机械臂标定的精确建模以及误差类型与辨识方法的合理匹配提供了理论依据。

关键词：机械臂标定 SD-H模型 MD-H模型欧拉角可辨识性

Engineering Heteronuclear Arrays from Ir^(Ⅲ)-Metalloligand and Co^(Ⅱ)Showing Coexistence of Slow Magnetization Relaxation and Photoluminescence

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Chinese Journal of Chemistry 2022年第8期40卷 931-938页

作者： Kun Fan song-song bao Zi-Wen Yu Xin-Da Huang Yu-Jie Liu Mohamedallly Kurmoo Li-Min Zheng State Key Laboratory of Coordination Chemistry Coordination Chemistry InstituteSchool of Chemistry and Chemical EngineeringCollaborative Innovation Center of Advanced MicrostructuresNanjing UniversityNanjingJiangsu 210023China Institut de Chimie Universitéde Strasbourg CNRS-UMR71774 rue Blaise PascalStrasbourg Cedex 67007France

Three heteronuclear Ir^(Ⅲ)-Co^(Ⅱ)arrays,[Co(H_(2)O)6]_(2)[Ir(ppyCOO)(ppyCOOH)(bpy)]_(2)[Ir(ppyCOO)_(2)(bpy)]_(2)(NO_(3))2·10.5H_(2)O(2),[Co(H_(2)O)6]{Co-(H_(2)O)4[Ir(ppyCOO)_(2)(bpy)]_(2)}[Ir(ppyCOO)_(2)(bpy)]2·15H_(2)O(3)and{Co(H_(2)O)2[Ir(ppyCOO)_(2)(bpy)]_(2)}·2CH_(3)OH·3H_(2)O(4),have been synthe-sized by combining paramagnetic Co^(Ⅱ)ion with luminescent Ir^(Ⅲ)-metalloligand,[Ir(ppyCOOH)_(2)(bpy)][Ir(ppyCOO)_(2)(bpy)]·5H_(2)O(1),where ppyCOOH=3-(pyridin-2-yl)benzoic acid and bpy=2,2'-*** to the variable charged states of compound 1 at dif-ferent pH,compounds 2 and 3 are discrete ionic complexes containing hydrated Co^(2+)cations and deprotonated[Ir(ppyCOO)_(2)(bpy)]-anions as well as coexisting neutral[Ir(ppyCOO)(ppyCOOH)(bpy)](for 2)or Co(H_(2)O)_(4)[Ir(ppyCOO)_(2)(bpy)]_(2) trinuclear molecule(for 3).Whereas compound 4 consists of self-assembled two-dimensional coordination polymer with Co-metalloligand dative *** hexa-coordinated Co^(Ⅱ)cores in all complexes display octahedral geometries with varying degrees of *** a bias dc field,the ac susceptibilities for 2-4 reveal slow magnetic ***,the solid-state phosphorescence is observed for compounds 2 and 3 due to weak supramolecular interaction between Co^(Ⅱ)ion and Ir-moiety but is severely quenched for 4,where the Co center is strongly connected to the *** the best of our knowledge,this is the first report of Ir-Co systems showing both slow magnetization relaxation and photoluminescence.

关键词： Crystal engineering Heterometallic complexes Iridium(Ⅲ)metalloligand Slow magnetization relaxation Luminescence