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Label-free superior contrast with c-band ultra-violet extinction microscopy

Light(science & Applications) 2023年第3期12卷 435-445页

作者： Florian ströhl Deanna L.Wolfson Ida s.Opstad Daniel H.Hansen Hong Mao balpreet s.ahluwalia Department of Physics and Technology UiT The Arctic University of NorwayTromsøNorway Department of Clinical science Intervention and TechnologyKarolinska InstituteStockholmSweden

In 1934,Frits Zernike demonstrated that it is possible to exploit the sample’s refractive index to obtain superior contrast images of biological *** refractive index contrast of a cell surrounded by media yields a change in the phase and intensity of the transmitted light *** change can be due to either scattering or absorption caused by the *** cells are transparent at visible wavelengths,which means the imaginary component of their complex refractive index,also known as extinction coefficient k,is close to ***,we explore the use of c-band ultra-violet(UVC)light for high-contrast high-resolution label-free microscopy,as k is naturally substantially higher in the UVC than at visible *** differential phase contrast illumination and associated processing,we achieve a 7-to 300-fold improvement in contrast compared to visible-wavelength and UVA differential interference contrast microscopy or holotomography,and quantify the extinction coefficient distribution within liver sinusoidal endothelial *** a resolution down to 215 nm,we are,for the first time in a far-field label-free method,able to image individual fenestrations within their sieve plates which normally requires electron or fluorescence superresolution *** illumination also matches the excitation peak of intrinsically fluorescent proteins and amino acids and thus allows us to utilize autofluorescence as an independent imaging modality on the same setup.

关键词： wave. extinction violet

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Case Report: Carnitine Palmitoyl Transferase II (CPT II) Deficiency

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Open Journal of Internal Medicine 2024年第1期14卷 93-101页

作者： Kathy Po Marius Chivu Edwin Rosas balpreet Kaur Department of Internal Medicine Saint James School of Medicine The Quarter Anguilla Department of Internal Medicine Medicine at University of Chicago Chicago USA Department of Internal Medicine Medical College of Wisconsin Wisconsin USA

Carnitine Palmitoyl Transferase II (CPTII) is a very important enzyme that helps with the oxidation of long-chain fatty acid to produce energy. Deficiency in CPTII will lead to energy deficiency in the case of fasting and the accumulation of the long chain fatty in the body. There are three types of CPT II deficiency, the myopathic form, the severe infantile hepatocardiomuscular form and the lethal neonatal form. They are all inherited as an autosomal recessive. Diagnosis of the CPTII are 1) tandem mass spectrometry (Ms/Ms) in adult form and 2) CPTII polymorphism (F352C), which is linked to reducing the activity of CPTII in infantile form [1]. Glucose is the primary management and medium-chain fatty acid is an alternative due to the bypass of the CPTII enzyme in the pathway. For the prevention of CPTII deficiency are to avoid long chain fatty acid (C12-fatty acid), fasting, prolonged exercise, known triggers, and certain medications such as anti-epileptics and general anesthesia. During the rhabdomyolysis and myoglobinuria attack, it is very important to maintain hydration to avoid acute renal failure. If, however, renal failure occurs, dialysis is recommended. We present a case of a 27-year-old African American woman with the significant past medical history of CPT II deficiency leading to recurrent rhabdomyolysis and myoglobinuria. Together with all the research studies from diagnosis to treatment of CPTII deficiency will help in clinical management of patients. And this case report will add to the existing case reports of patients who have CPTII deficiency in terms of how we diagnose, how we treat, and how we prevent symptoms from re-occurring.

关键词： Carnitine Palmitoyl Transferase II (CPTII) Mitochondria Long Chain Fatty Acid Medium Chain Fatty Acid Carnitine Carnitine Palmitoyl Transferase I (CPTI) Acyl-Carnitine Beta-Oxidation Rhabdomyolysis Myoglobinuria Renal Failure Hypoketotic Hypoglycemia

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Chip-based multimodal super-resolution microscopy for histological investigations of cryopreserved tissue sections

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Light(science & Applications) 2022年第3期11卷 35-51页

作者： Luis E.Villegas-Hernández Vishesh Dubey Mona Nystad Jean-Claude Tinguely David A.Coucheron Firehun T.Dullo Anish Priyadarshi sebastian Acuña Azeem Ahmad JoséM.Mateos Gery Barmettler Urs Ziegler Åsa Birna Birgisdottir Aud-Malin Karlsson Hovd Kristin Andreassen Fenton Ganesh Acharya Krishna Agarwal balpreet singh ahluwalia Department of Physics and Technology UiT The Arctic University of NorwayKlokkargårdsbakken N-9019TromsøNorway Department of Clinical Medicine Women’s Health and Perinatology Research GroupUiT The Arctic University of NorwayTromsøNorway Department of Obstetrics and Gynecology University Hospital of North NorwayTromsøNorway Center for Microscopy and Image Analysis University of ZurichZürichSwitzerland Division of Cardiothoracic and Respiratory Medicine University Hospital of North NorwayTromsøNorway Department of Clinical Medicine Clinical Cardiovascular Research GroupUiT The Arctic University of NorwayTromsøNorway Department of Medical Biology RNA and Molecular Pathology Research GroupUiT The Arctic University of NorwayTromsøNorway Division of Obstetrics and Gynecology Department of Clinical ScienceIntervention and TechnologyKarolinska InstituteStockholmSweden

Histology involves the observation of structural features in tissues using a *** diffraction-limited optical microscopes are commonly used in histological investigations,their resolving capabilities are insufficient to visualize details at subcellular *** a novel set of super-resolution optical microscopy techniques can fulfill the resolution demands in such cases,the system complexity,high operating cost,lack of multi-modality,and low-throughput imaging of these methods limit their wide adoption for histological *** this study,we introduce the photonic chip as a feasible high-throughput microscopy platform for super-resolution imaging of histological *** cryopreserved ultrathin tissue sections of human placenta,mouse kidney,pig heart,and zebrafish eye retina prepared by the Tokuyasu method,we demonstrate diverse imaging capabilities of the photonic chip including total internal reflection fluorescence microscopy,intensity fluctuation-based optical nanoscopy,single-molecule localization microscopy,and correlative light-electron *** results validate the photonic chip as a feasible imaging platform for tissue sections and pave the way for the adoption of super-resolution high-throughput multimodal analysis of cryopreserved tissue samples both in research and clinical settings.

关键词： resolution modal feasible

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