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2021中国畜牧兽医学会兽医寄生虫学分会第16次学术研讨会

作者： Penglong Wang Xiaoping Luo Junyan Li Yang Liu baihui teng Qin Du Rui Wang Xiaoye Yang

Nematodirus is a common gastrointestinal nematode in *** the process of longterm use of anthelmintic control,some strains will develop the resistance,resulting in a series of problems,such as the decrease of drug ***,the study of drug resistance of Nematodirus is great significance for the prevention and treatment of gastrointestinal nematodes in *** present, there are relatively few studies on ivermectin resistance of ***,in this paper,the ivermectin-resistant strain of Nematodirus in sheep were studied on the isolation,identification,cultivation,drug resistance detection and drug resistance-related P-gps gene expression in Inner Mongolia.（1） A longitudinal epidemiological investigations were performed on the infection of Nematodirus in sheep from six pastures in Xilingol and Hulunbuir grasslands,Inner *** results showed that pastures of Xilingol Blue Banner,the average infection rates of Nematodirus in spring and in autumn were 58.5% and 56.3% respectively,and in Hulunbuir Xinbarhu Right Banner,they were 41.7% and 55.6%,*** analysis showed that there was no significant difference in the infection rate of Nematodirus between two areas before deworming in spring and in autumn.（2） Through a series of isolation methods,a field strain of Nematodirus was obtained,and was identified by the combination of traditional morphology and molecular *** results indicated that the species was Nematodirus oiratianus.（3） The larvae development conditions of isolated nematode strain were optimized by orthogonal experiment,and the larvae were cultured under different pH,temperature,osmotic pressure and light time,and the results showed that the optimum conditions for its development were pH 5.5,temperature 27℃,osmotic pressure 0 mOsm/kg,and all light during the whole culture period.（4） The resistance of the strain to ivermectin was detected by larval migration inhibition *** data showed that the

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Study on Polymorphisms of GluCl-gbr-2b and P-gp-9 Genes of Ivermectin Resistant Strains in Haemonchus contortus

Study on Polymorphisms of GluCl-gbr-2b and P-gp-9 Genes of I...

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2021中国畜牧兽医学会兽医寄生虫学分会第16次学术研讨会

作者： Qin Du Penglong Wang Xiaoping Luo Junyan Li Yang Liu baihui teng Rui Wang Xiaoye Yang

The resistance of sheep gastrointestinal nematodes to anthelmintic drugs has become a common problem all over the world,among which the resistance of Haemonchus contortus to ivermectin is the most *** view of the need for the prevention and control of parasitic diseases in the production practice of sheep breeding industry,in this paper,the P-gp-9 gene and GluCl-gbr-2 b gene of native isolates of Haemonchus contortus were studied with international standard strains as control,in order to understand the mechanism of ivermectin *** results were as follows:The sequences of GluCl-gbr-2 b gene fragments of Haemonchus contortus were compared and analyzed by MEGA5.1 biological software,and it was found that 20 bases of GluCl-gbr-2 b gene fragments of international standard drug-resistant strain Hc-R and local drug-resistant isolates WSHc-001,WSHc-003 and WSHc-138 were common mutation points with international standard sensitive strain as ***,when the isolated sensitive strain YCHc-022 was used as control,the GluCl-gbr-2 b gene fragments of Hc-R,WSHc-001,WSHc-003 and WSHc-138 only had a common mutation at position 422,indicating that the resistance of Haemonchus contortus to ivermectin may be associated with a mutation at a base site on the GluCl-gbr-2 b gene fragment,and its nucleotide polymorphism may be used as a potential molecule markers for the detection of drug *** comparing and analyzing the ORF sequence of P-gp-9 gene,it was found that the ORF sequence of international standard sensitive strain Hc-S was identical with that of isolated sensitive strain YCHc-022,and the ORF sequence of international standard drug resistant strain Hc-R was also consistent with that of WSHc-001,WSHc-003 and ***,compared with the sensitive strain,the ORF nucleotide sequence of the P-gp-9 gene of the resistant strain mutated from CCA to CTA,and amino acid from proline to leucine at the 89 th *** the same time,the polymorphism of t

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Expression Analysis of PGP-A Gene in Ivermectin Resistant and Sensitive Strains of Haemonchus contortus

Expression Analysis of PGP-A Gene in Ivermectin Resistant an...

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2021中国畜牧兽医学会兽医寄生虫学分会第16次学术研讨会

作者： Minghua Yu baihui teng Yang Liu Penglong Wang Xiaoping Luo Junyan Li Qin Du Rui Wang Xiaoye Yang

Haemonchus contortus is the most common parasitie in ruminant breeding of animal *** contortus is mainly parasitic in the abomasum of ruminants,such as sheep and occasionally in the small *** infection rate is high,the infection intensity is high,and it is widely distributed,which has a great impact on the economic loss of animal *** a long time,the control of Haemonchus contortus almost mainly depends on chemical drugs,but long-term repeated blind drug use makes animals resistant to ivermectin and other ***,in order to explore the factors affecting the mechanism of drug resistance,the fluorescent quantitative PCR technique was used to compare the expression level of Pgp-A gene between standard drug-resistant strain(Hc-R) and standard sensitive strain(Hc-S) of Haemonchus contortus,and then analyze the experimental *** results show that Pgp-A gene is associated with drug resistance,which provides a basis for further understanding the mechanism of drug resistance.

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Analysis of Gene UGT Expression on Ivermectin Resistance and Sensitive Isolates in Haemchus contortus

Analysis of Gene UGT Expression on Ivermectin Resistance and...

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2021中国畜牧兽医学会兽医寄生虫学分会第16次学术研讨会

作者： Junqi Yang Yang Liu Penglong Wang Xiaoping Luo Junyan Li baihui teng Qin Du Rui Wang Xiaoye Yang

Haemonchus contortus,a gastrointestinal nematode,which parasitim in the abomasum of ruminants has high infection rate,large infection intensity and wide distribution,and has a great economic loss to animal husbandry in *** a long time,the prevention and control of Haemonchus contortus mainly rely on chemical drugs,but long-term repeated use resulted in resistance to ivermectin and other *** studies showed that the resistance of Haemonchus contortus to ivermectin was probably related to the UGT ***,in this study,the expression of UGT gene in infected larvae of different strains was compared by fluorescence quantitative PCR(RT qPCR).The results showed that the expression of UGT gene in resistant strain was significantly higher than that in susceptible strain and the results were consistent with those put forward by Ludmel Urdaneta-Maruez,which proved again that the UGT gene could be related to drug resistance.

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Detection of Ivermectin Resistance of Haemonchus contortus by Larval Migration Inhibition Test

Detection of Ivermectin Resistance of Haemonchus contortus b...

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2021中国畜牧兽医学会兽医寄生虫学分会第16次学术研讨会

作者： Yang Liu Xianling Xu Penglong Wang Xiaoping Luo Junyan Li baihui teng Qin Du Rui Wang Xiaoye Yang

The resistance of gastrointestinal nematodes to commonly used anthelmintic drugs has seriously affected the development of animal *** order to verify the larval migration inhibition test to detect the resistance of nematodes to ivermectin,the third-stage larvae of international standard strains of drug resistance and drug sensitivity of Haemonchus contortus to ivermectin were used as the research objects to prepare a series of different concentrations of ivermectin for larval migration inhibition *** data were analyzed by Graphpad Prism 5.0 software,and the drug concentrations of ivermectin inhibiting 50% larval migration(EC) and ivermectin inhibiting 90% larval migration(EC) were *** results showed that the ECvalue of international standard sensitive strain was 0.07 μg/mL,and EC value was 2.19 μg/mL;the EC value of the international standard drug resistant strain was 1.51 μg/mL,the EC value was 2.85 μg/mL,and the resistance rate（RR） was *** showed that the larval migration test could be used to detect the resistance of Haemonchus contortus to ivermectin.

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燃煤锅炉降低污染物排放措施探讨

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锅炉制造 2011年第3期 41-42页

作者：滕百慧祁哲哈尔滨锅炉厂有限责任公司黑龙江哈尔滨150046 锦西石化公司研究院辽宁葫芦岛125001

本文着重介绍降低锅炉燃烧产生的氮氧化物NOx的方法以及采取的措施。

关键词：锅炉降低NOx排放措施

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企业所得税税式支出的成本效益分析——以研发费用加计扣除政策为例

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金融研究 2024年第3期 188-206页

作者：刘柏惠中国社会科学院财经战略研究院北京100006

财政“紧平衡”背景下,企业减税降费需求和财政可持续性之间的矛盾日益凸显,需通过有效性评估加强税收优惠政策的科学性。本文从税式支出视角,测算企业所得税研发费用加计扣除政策的效益和成本。在效益分析中,采用双重差分模型衡量改革... 详细信息

财政“紧平衡”背景下,企业减税降费需求和财政可持续性之间的矛盾日益凸显,需通过有效性评估加强税收优惠政策的科学性。本文从税式支出视角,测算企业所得税研发费用加计扣除政策的效益和成本。在效益分析中,采用双重差分模型衡量改革对企业绩效的影响,估算额外税收收益;在成本分析中,参考国际普遍使用的测算思路,以及中国财政部门界定的测算方法,估算税收损失。结果发现:此政策使企业的总资产收益率提高1%,推动企业所得税规模上升440.5亿元,同时产生175.25亿元税收成本,成本收益率为251.36%。在提高样本代表性、改变因变量、考虑后续改革影响情况下,成本收益率分别为165.07%、248.84%、214.5%。本文还从经济增长、政策公平性、征纳成本等方面讨论了广义的政策效益和成本,证实了政策的有效性。本文有助于量化税式支出政策对财政收支的真实冲击,可为评估政策有效性和完善预算绩效管理理论框架提供线索,对进一步推动税式支出制度化建设具有参考价值。

关键词：税式支出成本效益评估加计扣除企业所得税

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藻油DHA微胶囊制备及稳态化递送技术研究进展

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中国食品学报 2024年第7期24卷 414-424页

作者：陈柏慧田方蔡路昀浙江海洋大学食品科学与药学学院、浙江省海产品健康危害因素关键技术研究重点实验室浙江舟山316022 浙江大学宁波研究院生物系统工程与食品科学学院浙江宁波315100

藻油DHA为多不饱和脂肪酸,具有促进大脑生长发育,预防心血管疾病,参与免疫调节等多种生理功能,然而其极不稳定,易受到光、热、水及CO_(2)等因素的影响,导致其氧化、变质。目前,国内外对藻油DHA稳态化递送技术的研究颇多,微胶囊技术是其... 详细信息

藻油DHA为多不饱和脂肪酸,具有促进大脑生长发育,预防心血管疾病,参与免疫调节等多种生理功能,然而其极不稳定,易受到光、热、水及CO_(2)等因素的影响,导致其氧化、变质。目前,国内外对藻油DHA稳态化递送技术的研究颇多,微胶囊技术是其中一种较为成熟的方法,且应用最为广泛,能够有效增强藻油DHA的递送能力、贮藏稳定性及生物利用度。本文概述藻油DHA的来源、理化性质、生物活性及其递送体系等,重点探讨藻油DHA微胶囊稳态化靶向递送技术,包括藻油DHA微胶囊的制备方法、递送体系、递送机制、影响因素等,旨在为拓宽藻油DHA在食品领域的应用范围,提供一定的理论依据。

关键词：藻油DHA 微胶囊喷雾干燥法稳态化递送体系

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评价构式“(你/您)看/瞧人家NP”的演变及其功能浮现

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当代修辞学 2024年第5期 49-59页

作者：陈昌来宁柏慧上海师范大学对外汉语学院上海200234

“(你/您)看/瞧人家NP”形式上来源于清末民初含“看”类话语提示成分的定语领属式和同位同指式的“人家+NP”,由源构式重组而成,形式异变、频率效应、类推机制促动其形成。意义上经历了“领属限定”>“主观评价”的动态范畴化和主观化... 详细信息

“(你/您)看/瞧人家NP”形式上来源于清末民初含“看”类话语提示成分的定语领属式和同位同指式的“人家+NP”,由源构式重组而成,形式异变、频率效应、类推机制促动其形成。意义上经历了“领属限定”>“主观评价”的动态范畴化和主观化过程,体现了“领属”范畴>“立场”范畴的这一重要语义演变路径,隐喻机制在其中发挥作用。从共时角度看,语境、构件因素、语用移情策略等影响构式立场表达功能的浮现;构式重音策略影响构式强调突显功能的浮现;语言经济原则、礼貌原则等影响构式语用修辞功能的浮现。

关键词：评价构式类型演变功能浮现

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一种考虑裂缝扩展路径的混凝土细观数值模拟方法

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土木工程学报 2024年第3期57卷 30-41页

作者：李冬黄百惠王苑佐金浏杜修力北京工业大学城市与工程安全减灾教育部重点实验室北京100124

文章提出一种能够考虑混凝土裂缝扩展过程中绕过骨料或穿过骨料发展的细观数值模拟方法。首先,回顾了用于类混凝土材料I-型断裂分析的自洽有限应力模型的基本原理;进而,采用有限元分析方法,从局部能量等效和宏观力学等效两个维度验证了... 详细信息

文章提出一种能够考虑混凝土裂缝扩展过程中绕过骨料或穿过骨料发展的细观数值模拟方法。首先,回顾了用于类混凝土材料I-型断裂分析的自洽有限应力模型的基本原理;进而,采用有限元分析方法,从局部能量等效和宏观力学等效两个维度验证了基于自洽有限应力原理建模和常规建模之间的等效性;最终,发展了基于自洽有限应力原理和细观裂缝扩展路径准则的数值模拟方法,在细观尺度上实现模拟混凝土裂缝绕过骨料发展和穿过骨料发展等细观断裂破坏行为。文章研究成果可为后续发展基于细观尺度断裂破坏准则的高效数值分析方法奠定基础。

关键词：混凝土断裂细观尺度有限元分析自洽有限应力原理

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