Isolation and P-gps Gene Expression Analysis on Ivermectin-resistant Strain of Nematodirus oiratianus in Sheep
会议名称：《2021中国畜牧兽医学会兽医寄生虫学分会第16次学术研讨会》
会议日期：2021年
学科分类：090601[农学-基础兽医学] 09[农学] 0906[农学-兽医学]
摘 要：Nematodirus is a common gastrointestinal nematode in *** the process of longterm use of anthelmintic control,some strains will develop the resistance,resulting in a series of problems,such as the decrease of drug ***,the study of drug resistance of Nematodirus is great significance for the prevention and treatment of gastrointestinal nematodes in *** present, there are relatively few studies on ivermectin resistance of ***,in this paper,the ivermectin-resistant strain of Nematodirus in sheep were studied on the isolation,identification,cultivation,drug resistance detection and drug resistance-related P-gps gene expression in Inner Mongolia.（1） A longitudinal epidemiological investigations were performed on the infection of Nematodirus in sheep from six pastures in Xilingol and Hulunbuir grasslands,Inner *** results showed that pastures of Xilingol Blue Banner,the average infection rates of Nematodirus in spring and in autumn were 58.5% and 56.3% respectively,and in Hulunbuir Xinbarhu Right Banner,they were 41.7% and 55.6%,*** analysis showed that there was no significant difference in the infection rate of Nematodirus between two areas before deworming in spring and in autumn.（2） Through a series of isolation methods,a field strain of Nematodirus was obtained,and was identified by the combination of traditional morphology and molecular *** results indicated that the species was Nematodirus oiratianus.（3） The larvae development conditions of isolated nematode strain were optimized by orthogonal experiment,and the larvae were cultured under different pH,temperature,osmotic pressure and light time,and the results showed that the optimum conditions for its development were pH 5.5,temperature 27℃,osmotic pressure 0 mOsm/kg,and all light during the whole culture period.（4） The resistance of the strain to ivermectin was detected by larval migration inhibition *** data showed that the