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Tongqiao Yizhi granule(通窍益智颗粒) repress the nuclear factor kappa-b/nucleotide oligomerization domain-like receptors 3/caspase-1 pyroptosis pathway in the hippocampus to counter vascular dementia in rats

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Journal of Traditional Chinese Medicine 2024年第4期 680-687页

作者： LI Yanjiao HAO Linyao LI Shuangyang LUO Yanqi WANG Juan WANG Raoqiong bai xue College of Integrative Medicine Southwest Medical University Department of Neurology and National Traditional Chinese Medicine Clinical Research Base the Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University Institute of Integrated Chinese and Western Medicine Southwest Medical University

OBJECTIVE: To explore the mechanism by which Tongqiao Yizhi granule（通窍益智颗粒, TQYZKL） intervenes pyroptosis to treat vascular dementia（VaD） in a rat model. METHODS: The rat model of VaD was established by two-vessel occlusion（2VO）. The rats were randomly divided into Sham group, Model group, Nimodipine group, TQYZKL（6.2 g·kg-1·d-1）, TQYZKL(12.4 g·kg-1·d-1), TQYZKL(24.8 g·kg-1·d-1). The Morris water maze（MWM） test was carried out to test the learning and memory function; Hematoxylin-eosin staining and transmission electron microscopy（TEM） to observe the pathological damage in the hippocampus; Tunel fluorescence staining to detect neuronal pyroptosis in the hippocampus. The expression levels of pyroptosis-related proteins, namely Golgi peripheral membrane protein p65（P65）, nucleotide oligomerization domain-like receptors 3（NLRP3）, caspase-1 and Gasdermin D（GSDMD）, were detected using Western blotting and reverse transcription polymerase chain reaction. Moreover, the serum levels of interleukin-1β（IL-1β） and interleukin-18（IL-18） were determined through the enzyme-linked immunosorbent assay. RESULTS: The study revealed that TQYZKL effectively improved the ability of VaD ratsto learn and memorize, relieved the pathological damage in the hippocampus, restored neuronal morphology, and reduced the expression of pyroptosis-related proteins P65, NLRP3, caspase-1, GSDMD-N, IL-18 and IL-1β（P < 0.05）. CONCLUSION: TQYZKL inhibits neuronal pyroptosis in the hippocampus of VaD rats by regulating nuclear factor kappa-B/NLRP3/caspase-1 signaling pathway, thus exerting a therapeutic effect on VaD in the rats.

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Direct reprogramming of fibroblasts into functional hepatocytes via CRISPRa activation of endogenous Gata4 and Foxa3

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中华医学杂志(英文版) 2024年第11期137卷 2024 May 9页

作者： Li Jiacheng Li Ruopu bai xue Zhang Wenlong Nie Yu Hu Shengshou State Key Laboratory of Cardiovascular Disease Fuwai Hospital and Cardiovascular Institute National Center for Cardiovascular Diseases Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China Department of Obstetrics and Gynecology Beijing Advanced Innovation Center for Genomics Third Hospital School of Life Sciences Peking University Beijing China

Background: The ability to generate functional hepatocytes without relying on donor liver organs holds significant therapeutic promise in the fields of regenerative medicine and potential liver disease treatments. Clustered regularly interspaced short palindromic repeats (CRISPR) activator (CRISPRa) is a powerful tool that can conveniently and efficiently activate the expression of multiple endogenous genes simultaneously, providing a new strategy for cell fate determination. The main purpose of this study is to explore the feasibility of applying CRISPRa for hepatocyte reprogramming and its application in the treatment of mouse liver fibrosis.Method: The differentiation of mouse embryonic fibroblasts (MEFs) into functional induced hepatocyte-like cells (iHeps) was achieved by utilizing the CRISPRa synergistic activation mediator (SAM) system, which drove the combined expression of three endogenous transcription factors—Gata4,Foxa3, andHnf1a—or alternatively, the expression of two transcription factors,Gata4 andFoxa3.In vivo, we injected adeno-associated virus serotype 6 (AAV6) carrying the CRISPRa SAM system into liver fibroticCol1a1-CreER;Cas9fl/fl mice, effectively activating the expression of endogenousGata4 andFoxa3 in fibroblasts. The endogenous transcriptional activation of genes was confirmed using real-time quantitative polymerase chain reaction (RT-qPCR) and RNA-seq, and the morphology and characteristics of the induced hepatocytes were observed through microscopy. The level of hepatocyte reprogrammingin vivo is detected by immunofluorescence staining, while the improvement of liver fibrosis is evaluated through Sirius red staining, alpha-smooth muscle actin (α-SMA) immunofluorescence staining, and blood alanine aminotransferase (ALT) examination.Results: Activation of only two factors,Gata4 andFoxa3, via CRISPRa was sufficient to successfully induce the transformation of MEFs into iHeps. These iHeps could be expandedin vitro and displayed functional characteristics similar to those of mature hepatocyte

关键词： Liver fibrosis CRISPRa Cell reprogramming Transcriptional activation Hepatocytes

Free-moving-state microscopic imaging of cerebral oxygenation and hemodynamics with a photoacoustic fiberscope

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Light(Science & Applications) 2024年第1期13卷 33-46页

作者： Xiaoxuan Zhong Yizhi Liang Xiaoyu Wang Haoying Lan xue bai Long Jin bai-Ou Guan Guangdong Provincial Key Laboratory of Optical Fiber Sensing and Communications Institute of Photonics TechnologyJinan UniversityGuangzhou510632China

We report the development of a head-mounted photoacoustic fiberscope for cerebral imaging in a freely behaving mouse.The 4.5-gram imaging probe has a 9-µm lateral resolution and 0.2-Hz frame rate over a 1.2-mm wide area.The probe can continuously monitor cerebral oxygenation and hemodynamic responses at single-vessel resolution,showing significantly different cerebrovascular responses to external stimuli under anesthesia and in the freely moving state.For example,when subjected to high-concentration CO_(2) respiration,enhanced oxygenation to compensate for hypercapnia can be visualized due to cerebral regulation in the freely moving state.Comparative studies exhibit significantly weakened compensation capabilities in obese rodents.This new imaging modality can be used for investigating both normal and pathological cerebrovascular functions and shows great promise for studying cerebral activity,disorders and their treatments.

关键词： cerebral anesthesia moving

Erratum to:CD146 as a promising therapeutic target for retinal and choroidal neovascularization diseases

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Science China(Life Sciences) 2024年第2期67卷 434-434页

作者： bai xue Ping Wang Wenzhen Yu Jing Feng Jie Li Rulian Zhao Zhenglin Yang Xiyun Yan Hongxia Duan Sichuan Provincial Key Laboratoryfor Human Disease Gene Study Sichuan Provincial People'sHospitalSchoolof MedicineUniversity of Electronic Science and Technology of ChinaChengdu 610054China Key Laboratory of Protein and Peptide Pharmaceutical Institute of BiophysicsChinese Academy of SciencesBeijing 100101China Department of Ophthalmology People's HospitalPeking UniversityBeijing 100044China Department of Ophthalmology Sichuan Provincial People's HospitalUniversity of Electronic Science and Technology of ChinaChengdu 61072China Research Unit for Blindness Prevention of Chinese Academy of Medical Sciences(2019RU026) Sichuan Academy of Medical Sciences and Sichuan Provincial People's HospitalChengdu 610072China Nanozyme Medical Center School of Basic Medical SciencesZhengzhou UniversityZhengzhou 450001China College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China

Erratum to:Sci China Life Sciences,Volume 65,Issue 6:1157-1170(2022),https://***/10.1007/s11427-021-2020-0This paper contains four errors in Figure 4 and Figure 5.In Figure 4C,the image labeled as"1%O_(2)+IgG ctrl"is incorect.This image is another one for 1%O_(2) group.We provide a new image as follows for 1%O_(2)+IgG ctrl group.This new image does not affect the conclusion of this article.

关键词： Figure image CD146

Evolution of pore systems in low-maturity oil shales during thermal upgrading——Quantified by dynamic SEM and machine learning

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Petroleum Science 2024年第3期21卷 1739-1750页

作者： Jun Liu xue bai Derek Elsworth Key Laboratory of Deep Underground Science and Engineering(Ministry of Education) Institute of New Energy and Low-Carbon Technology Sichuan University International Quantum Academy Shenzhen Institute of Advanced Electronic Materials Shenzhen Institute of Advanced Technology Chinese Academy of Sciences Department of Energy and Mineral Engineering Pennsylvania State University

In-situ upgrading by heating is feasible for low-maturity shale oil, where the pore space dynamically evolves. We characterize this response for a heated substrate concurrently imaged by SEM. We systematically follow the evolution of pore quantity, size(length, width and cross-sectional area), orientation, shape(aspect ratio, roundness and solidity) and their anisotropy—interpreted by machine learning. Results indicate that heating generates new pores in both organic matter and inorganic minerals. However, the newly formed pores are smaller than the original pores and thus reduce average lengths and widths of the bedding-parallel pore system. Conversely, the average pore lengths and widths are increased in the bedding-perpendicular direction. Besides, heating increases the cross-sectional area of pores in low-maturity oil shales, where this growth tendency fluctuates at 300°C. In addition, the orientation and shape of the newly-formed heating-induced pores follow the habit of the original pores and follow the initial probability distributions of pore orientation and shape. Herein, limited anisotropy is detected in pore direction and shape, indicating similar modes of evolution both bedding-parallel and bedding-normal. We propose a straightforward but robust model to describe evolution of pore system in low-maturity oil shales during heating.

关键词： Low-maturity oil shale Pore elongation Organic matter pyrolysis In-situ thermal upgrading Scanning electron microscopy (SEM) Machine learning

Surface reconstruction and structural transformation of twodimensional Ni-Fe MOFs for oxygen evolution in seawater media

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Nano Research 2024年第4期17卷 2429-2437页

作者： Liyuan Xiao xue bai Jingyi Han Tianmi Tang Siyu Chen Hui Qi Changmin Hou Fuquan bai Zhenlu Wang Jingqi Guan Institute of Physical Chemistry College of ChemistryJilin UniversityChangchun 130021China The Second Hospital of Jilin University Changchun 130021China State Key Laboratory of Inorganic Synthesis and Preparative Chemistry College of ChemistryJilin UniversityChangchun 130012China Laboratory of Theoretical and Computational Chemistry Institute of Theoretical ChemistryCollege of ChemistryChangchun 130023China

As a four-electron transfer reaction,oxygen evolution reaction(OER)is limited by large overpotential and slow kinetics.Here,we in-situ synthesized two-dimensional(2D)Ni-Fe metal-organic framework nanosheets on nickel foam(NixFe-TPA/NF,TPA=terephthalic acid)for oxygen evolution in alkaline and alkaline seawater electrolytes.In 1 M KOH,Ni3Fe-TPA/NF shows a low overpotential(η10)of 189 mV at 10 mA·cm^(-2) and an ultra-low overpotential of only 260 mV at 500 mA·cm^(-2).In alkaline seawater,Ni3Fe-TPA/NF still provides impressive OER performance,with anη10 of 265 mV.In-situ Raman characterization results show that the phase transition occurs during the OER,and Ni3FeOOH with more oxygen vacancies is in-situ formed,reducing the OER energy barrier.Density functional theory(DFT)reveals that the synergy between Ni and Fe reduces the energy barrier and accelerates the rate-determining step.In addition,the ultra-thin 2D sheet structure and the close combination of Ni3FeOOH and highly conductive NF support ensure the high catalytic OER activity.Therefore,the surface reconstruction and structural modification strategy can be used to design and prepare high-performance OER electrocatalysts for energy-related applications.

关键词： density functional theory(DFT)calculation Ni3FeOOH oxygen evolution reaction seawater electrolysis surface reconstruction

Rare earth inorganic-organic hybrid compounds based on Keggin-type polyoxometalate {SiW_(12)} with fast-responsive photochromism and switchable luminescence properties

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Journal of Rare Earths 2024年第2期42卷 286-292,I0003页

作者： Hong Cui Yanli Yang xue bai Xu Han Wensha Zhang Ying Lu Shuxia Liu Key Laboratory of Polyoxometalate and Reticular Material Chemistry of the Ministry of Education College of ChemistryNortheast Normal UniversityChangchun 130024China

Four rare earth inorganic-organic hybrid compounds based on Keggin-type polyoxometalate {SiW_(12)}with general formula [{RE(DMF)_(4)(H_(2)O)_4RE(DMF)_(6)}_(2){SiW_(12)O_(40)}_(3)](RE = La(1),Pr(2),Sm(3),Eu(4),DMF=N,N-dimethylformamide) were synthesized by the conventional solution method.Structure analyses indicate that four compounds are isostructural and are all built from three [SiW_(12)O_(40)]^(4-) polyoxoanions and DMF ligands linked by RE^(3+).Compounds 1-4 are extremely sensitive to UV light and present excellent photochromic properties,in which the coloring and fading time of samples are both no more than 2 min,and the reversible coloring-fading process can be repeated for at least 10 cycles with little fatigue,suggesting that compounds 1-4 can be a series of fast-responsive and durable photochromic materials.Moreover,the solid-state photoluminescence spectra of compounds 3(Sm) and 4(Eu)display strong characteristic emissions of rare earth ions based on f-f transitions.Meanwhile,compound4(Eu) has a longer fluorescence lifetime up to 566.74 μs.Notably,co mpounds 3(Sm) and 4(Eu) exhibit the switchable luminescence behavior induced by photochromism,which allows them to be used as potential molecular switches.

关键词： Inorganic-organic hybrid Keggin-type polyoxometalates Rare earths Photochromism Photoluminescence

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Random Exponential Attractor for Stochastic Non-autonomous Suspension Bridge Equation with Linear Multiplicative White Noise

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Journal of Mathematical Research with Applications 2024年第1期44卷 81-112页

作者： Ling XU Runjie LIU xue bai College of Mathematics and Statistics Northwest Normal University

In this paper, we mainly consider the long-time behavior of stochastic non-autonomous suspension bridge equation by linear multiplicative white noise with small coefficient. First step,the well-posedness for the cocycle associated with the considered system is established. Second step, the existence of random attractor for the system is investigated. Third step, the upper semicontinuity of random attractor is also provided when the coefficient of random term approaches zero. Fourth step, we prove the regularity of random attractor in a higher regular space by the“iteration” method. Finally, we give the existence of a random exponential attractor for the considered system, which implies the finiteness of fractal dimension of random attractor.

关键词： stochastic non-autonomous suspension bridge equation multiplicative white noise random attractor fractal dimension random exponential attractor

Frequency-comb-linearized, widely tunable lasers for coherent ranging

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Photonics Research 2024年第4期12卷 663-681页

作者： BAOQI SHI YI-HAN LUO WEI SUN YUE HU JINBAO LONG xue bai ANTING WANG JUNQIU LIU Department of Optics and Optical Engineering University of Science and Technology of ChinaHefei 230026China International Quantum Academy Shenzhen 518048China Shenzhen Institute for Quantum Science and Engineering Southern University of Science and TechnologyShenzhen 518055China Hefei National Laboratory University of Science and Technology of ChinaHefei 230088China

Tunable lasers,with the ability to continuously vary their emission wavelengths,have found widespread applications across various fields such as biomedical imaging,coherent ranging,optical communications,and spectroscopy.In these applications,a wide chirp range is advantageous for large spectral coverage and high frequency resolution.Besides,the frequency accuracy and precision also depend critically on the chirp linearity of the laser.While extensive efforts have been made on the development of many kinds of frequency-agile,widely tunable,narrow-linewidth lasers,wideband yet precise methods to characterize and linearize laser chirp dynamics are also demanded.Here we present an approach to characterize laser chirp dynamics using an optical frequency comb.The instantaneous laser frequency is tracked over terahertz bandwidth at 1 MHz intervals.Using this approach we calibrate the chirp performance of 12 tunable lasers from Toptica,Santec,New Focus,EXFO,and NKT that are commonly used in fiber optics and integrated photonics.In addition,with acquired knowledge of laser chirp dynamics,we demonstrate a simple frequency-linearization scheme that enables coherent ranging without any optical or electronic linearization unit.Our approach not only presents novel wideband,highresolution laser spectroscopy,but is also critical for sensing applications with ever-increasing requirements on performance.

关键词： tunable Focus coherent

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