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人PD-1分子基因克隆及其在COS-7细胞中表达的研究

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西北国防医学杂志 2003年第4期24卷 250-252页

作者：张华欣白云姜曼黎万玲第三军医大学分子遗传教研室重庆400038

目的 :克隆人PD - 1分子的编码序列cDNA ,将其重组进真核表达载体中 ,并表达于COS - 7细胞。方法 :从活化的人T细胞cDNA文库中以PCR方法克隆编码人PD - 1的编码cDNA序列 ,将其克隆进真核表达载体pcDNA3.1 (+) ,构建成重组表达载体 ,并... 详细信息

目的 :克隆人PD - 1分子的编码序列cDNA ,将其重组进真核表达载体中 ,并表达于COS - 7细胞。方法 :从活化的人T细胞cDNA文库中以PCR方法克隆编码人PD - 1的编码cDNA序列 ,将其克隆进真核表达载体pcDNA3.1 (+) ,构建成重组表达载体 ,并测序证实。用脂质体法转染COS - 7细胞 ,流式细胞仪检测PD - 1分子的膜表达。结果 :PCR方法扩增出一 880bp左右的基因片段 ,插入pcDNA3.1 (+)载体后构建成重组表达质粒 ,经测序证实扩增的片段为人PD - 1编码序列。将重组质粒转染COS - 7细胞 ,流式细胞仪检测显示有 2.1 .1 0 %的细胞表达人PD - 1分子。结论 :本研究为进一步研究PD - 1分子在免疫耐受。

关键词： PD—1 COS—7细胞基因表达

Mechanism underlying the anterior insula and its related neural pathway involved in the transmission and modulation of pain

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Mechanism underlying the anterior insula and its related neu...

中国解剖学会2.2.年年会

作者： bai Yang Chen Yanbing Fan Boyuan Li Yunqing department of Anatomy Histology and EmbryologyThe Fourth Military Medical University

In recent years,the role of insular cortex (IC) in pain sensation gains more and more *** on its anatomical connection feature, it is generally conceived that anterior insula (aIC) is involved in emotional aspectand posterior insula (pIC) is related to sensory aspect of pain,***,functional evidence is still lacking to support this hypothesis.

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Robust Control Using Self Recurrent Wavelet Neural Network for a Coaxial Eight-Rotor UAV with Uncertainties

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Robust Control Using Self Recurrent Wavelet Neural Network f...

2.15年中国智能自动化学术会议

作者： Cheng Peng Yue bai Xun Gong Yantao Tian department of Control Science and Engineering Jilin University Chinese Academy of Sciences Changchun Institute of Optics Fine Mechanics and Physics

This paper focuses on the robust control of a coaxial eight-rotor UAV in the presence of model uncertainties and external disturbances. The dynamical and kinematical model of the eight-rotor with high drive capability is established. On account of the uncertainties, a robust back-stepping sliding mode control(BSMC)with self-recurrent wavelet neural network(SRWNN) method is proposed as the attitude controller of the eight-rotor. SRWNN as the uncertainty observer can effectively estimate the lumped uncertainties. All weights of SRWNN can be trained online by the adaptation laws based on Lyapunov stability theorem. Then the uniformly ultimate stability of the eight-rotor system is proved. Finally, simulation results demonstrate the validity of the proposed robust control method adopted in the eight-rotor under model uncertainties and external disturbances.

关键词： Coaxial eight-rotor UAV Self-recurrent wavelet neural network Uncertainties External disturbances

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激光技术及强激光与物质相互作用实验

物理实验 2003年第2期23卷 6-8页

作者：夏小平王声波吴鸿兴郭大浩戴宇生范勇吴柏枚中国科技大学物理系安徽合肥230026

利用科研成果 ,开设激光技术及强激光与物质相互作用实验 ,让学生了解强激光与物质相互作用时的2.典型效应 :二次非线性光学效应 ,高压冲击波 (光力学 )效应 ,实现了科研与实验教学的紧密结合 .

关键词：激光技术实验强激光非线性光学高压冲击波相互作用

High-resolution genetic mapping and identification of candidate genes for the wheat stem rust resistance gene Sr8155B1

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The Crop Journal 2023年第6期11卷 1852-1861页

作者： Jian Wang Hongyu Li Tao Shen Shikai Lyu Shams ur Rehman Hongna Li Guiping Wang Binyang Xu Qing Wang Wanyi Hu Kairong Li Shengsheng bai Jian Ma Haitao Yu Matthew N.Rouse Shisheng Chen School of Advanced Agriculture Sciences Peking UniversityBeijing 100871China National Key Laboratory of Wheat Improvement Peking University Institute of Advanced Agricultural SciencesShandong Laboratory of Advanced Agricultural Sciences in WeifangWeifang 261325ShandongChina Triticeae Research Institute Sichuan Agricultural UniversityChengdu 611130SichuanChina Wheat Research Institute Weifang Academy of Agricultural SciencesWeifang 261071ShandongChina US department of Agriculture-Agricultural Research Service Cereal Disease Laboratory and Department of Plant PathologyUniversity of MinnesotaSt.PaulMN 55108USA

Stem rust,caused by Puccinia graminis ***(Pgt),threatens global wheat *** of cultivars with increased resistance to stem rust by identification,mapping,and deployment of resistance genes is the best strategy for controlling the *** this study,we performed fine mapping and characterization of the all-stage stem rust resistance(Sr)gene Sr8155B1 from the durum wheat line *** seedling tests of biparental populations,Sr8155B1 was effective against six Chinese Pgt races *** a segregating population of 5060 gametes,Sr8155B1 was mapped to a 0.06-cM region flanked by markers Pku2.72.and Pku43365,corresponding to 1.5-and 2.7-Mb regions in the Svevo and Chinese Spring reference *** regions include several typical nucleotide-binding leucine-rich repeat(NLR)and protein kinase genes that represent candidate *** them,three NLR genes and three receptor-like protein kinases were highly polymorphic between the parental lines and their transcripts were upregulated in the homozygous resistant line TdR2.relative to its susceptible sister line *** markers(Pku2.72.Pku43365,Pku2.50,and Pku372.)developed in this study,together with seedling resistance responses,correctly predicted Sr8155B1 absence or presence in 78 tetraploid wheat genotypes *** presence of Sr8155B1 in tetraploid wheat accessions CItr 14916,PI 197492.and PI 197493 was confirmed by mapping in three F_(2.*** genetic map and linked markers developed in this study may accelerate the deployment of Sr8155B1-mediated resistance in wheat breeding programs.

关键词： Durum wheat Stem rust Resistance gene Sr8155B1 CC-NBS-LRR

Overexpression of Lrp5 enhanced the anti-breast cancer effects of osteocytes in bone

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Bone Research 2021年第4期9卷 494-506页

作者： Shengzhi Liu Di Wu Xun Sun Yao Fan Rongrong Zha Aydin Jalali Yan Feng Kexin Li Tomohiko Sano Nicole Vike Fangjia Li Joseph Rispoli Akihiro Sudo Jing Liu Alexander Robling Harikrishna Nakshatri bai-Yan Li Hiroki Yokota department of Biomedical Engineering Indiana University Purdue University IndianapolisIndianapolisINUSA department of Pharmacology School of PharmacyHarbin Medical UniversityHarbinChina department of Pharmacy The Second Affiliated Hospital of Dalian Medical UniversityDalianChina department of Orthopedic Surgery Mie UniversityTsuMieJapan Weldon School of Biomedical Engineering Purdue UniversityWest LafayetteINUSA department of Physics Indiana University Purdue University IndianapolisIndianapolisINUSA department of Anatomy and Cell Biology Indiana University School of MedicineIndianapolisINUSA Indiana Center for Musculoskeletal Health Indiana University School of MedicineIndianapolisINUSA department of Surgery Indiana University School of MedicineIndianapolisINUSA Simon Cancer Center Indiana University School of MedicineIndianapolisINUSA

Osteocytes are the most abundant cells in bone,which is a frequent site of breast cancer ***,we focused on Wnt signaling and evaluated tumor-osteocyte *** animal experiments,mammary tumor cells were inoculated into the mammary fat pad and *** role of Lrp5-mediated Wnt signaling was examined by overexpressing and silencing Lrp5 in osteocytes and establishing a conditional knockout mouse *** results revealed that administration of osteocytes or their conditioned medium(CM)inhibited tumor progression and *** overexpressing Lrp5 or β-catenin displayed strikingly elevated tumor-suppressive activity,accompanied by downregulation of tumor-promoting chemokines and upregulation of apoptosis-inducing and tumor-suppressing proteins such as *** antitumor effect was also observed with osteocyte-derived CM that was pretreated with a Wnt-activating ***,silencing Lrp5 in tumors inhibited tumor progression,while silencing Lrp5 in osteocytes in conditional knockout mice promoted tumor *** exhibited elevated Lrp5 expression in response to tumor cells,implying that osteocytes protect bone through canonical Wnt ***,our results suggest that the Lrp5/β-catenin axis activates tumor-promoting signaling in tumor cells but tumor-suppressive signaling in *** envision that osteocytes with Wnt activation potentially offer a novel cell-based therapy for breast cancer and osteolytic bone metastasis.

关键词： cytes elevated inhibited

Clinical usefulness of linked color imaging in identifying Helicobacter pylori infection:A systematic review and meta-analysis

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World Journal of Gastrointestinal Endoscopy 2023年第12期15卷 735-744页

作者： Yu Zhang Jing-Zhai Wang Xuan bai Peng-Li Zhang Qiang Guo department of Kunming university of Science and Technology Kunming 650000Yunnan ProvinceChina 不详 department of Gastroenterology The First People’s Hospital of Yunnan ProvinceAffiliated Hospital of Kunming University of Science and TechnologyKunming 650000Yunnan ProvinceChina department of Gastr-oenterology The First People’s Hospital of Yunnan ProvinceAffiliated Hospital of Kunming University of Science and TechnologyKunming 650000Yunnan ProvinceChina

BACKGROUND Accurate diagnosis of Helicobacter pylori(***)infection status is a crucial premise for eradication therapy,as well as evaluation of risk for gastric *** progress on imaging enhancement endoscopy(IEE)made it possible to not only detect precancerous lesions and early gastrointestinal cancers but also to predict *** infection in real *** a novel IEE modality,linked color imaging(LCI)has exhibited its value on diagnosis of lesions of gastric mucosa through emphasizing minor differences of color *** To compare the efficacy of LCI for *** active infection vs conventional white light imaging(WLI).METHODS PubMed,Embase,Embase and Cochrane Library were searched up to the end of April 11,*** random-effects model was adopted to calculate the diagnostic efficacy of LCI and *** calculation of sensitivity,specificity,and likelihood ratios were performed;symmetric receiver operator characteristic(SROC)curves and the areas under the SROC curves were *** of the included studies was chosen to assess using the quality assessment of diagnostic accuracy studies-2.*** Seven original studies were included in this *** pooled sensitivity,specificity,positive likelihood rate,and negative likelihood rate of LCI for the diagnosis of *** infection of gastric mucosa were 0.85[95%confidence interval(CI):0.76-0.92.,0.82.95%CI:0.78-0.85),4.71(95%CI:3.7-5.9),and 0.18(95%CI:0.10-0.31)respectively,with diagnostic odds ratio=2.(95%CI:13-52.,SROC=0.87(95%CI:0.84-0.90),which showed superiority of diagnostic efficacy compared to *** Our results showed LCI can improve efficacy of diagnosis on *** infection,which represents a useful endoscopic evaluation modality for clinical practice.

关键词： Helicobacter pylori infection Endoscopic diagnosis Linked color imaging Gastric cancer Meta-analysis

Gaze Tracking System for Teleoperation

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Gaze Tracking System for Teleoperation

第2.届中国控制与决策会议

作者： Mingxin Yu Xiangzhou Wang Yingzi Lin Xiaoping bai School of Automation Beijing Institute of Technology department of Mechanical and Industrial Engineering Northeastern University

Conventional control interfaces such as joystick, keyboard, and mouse are widely used in teleoperation. However, they are not operated by some users such as who have disabled hands. This paper proposed an eye gaze tracking system for teleoperation. The eye gaze tracking system is based on non-intrusive, visible light, iris center corneal reflection（ICCR） and 2.D mapping-based gaze estimation. In addition, this paper also presented a simple and effective 2.D mapping-based calibration method in order to users with head movement. The calibration verification of five users shows the average errors of our proposed gaze tracker on the x- and y directions are 13.3 and 16.4 pixels, respectively. Through the teleoperation test of robotic car within fixed experimental area, the gaze tracking system achieved a good control result.

关键词： gaze tracking ICCR teleoperation

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合成氨EDA催化剂模型化合物的量化计算

厦门大学学报(自然科学版) 1980年第4期 118-122页

作者：赖伍江黄泰山白震谷张平厦门大学物理化学研究所澳门大学数学系

一、引言 1973年在我国第二次固氮会议上,许多单位在日本田丸合成氨EDA催化剂的基础上,提出了以碱金属Na为电子授主的EDA体系催化合成氨的报告,活性都比田丸的高。1974日本Ozaki又报导了钌——钾EDA体系催化合成氨的文章。我国固氮EDA... 详细信息

一、引言 1973年在我国第二次固氮会议上,许多单位在日本田丸合成氨EDA催化剂的基础上,提出了以碱金属Na为电子授主的EDA体系催化合成氨的报告,活性都比田丸的高。1974日本Ozaki又报导了钌——钾EDA体系催化合成氨的文章。我国固氮EDA协作组采用金属钾的EDA催化剂,得到比金属Na高得多的合成氨的活性。

关键词：化合物催化剂 EDA 量化计算