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Comprehensive transcriptional atlas of human adenomyosis deciphered by the integration of single-cell RNA-sequencing and spatial transcriptomics

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Protein & Cell 2024年第7期15卷 530-546页

作者： Tao Chen Yiliang Xu Xiaocui Xu Jianzhang Wang Zhiruo Qiu Yayuan Yu Xiaohong Jiang Wanqi Shao Dandan bai Mingzhu Wang Shuyan Mei Tao Cheng Li Wu Shaorong gao Xuan Che Department of Obstetrics and Gynecology Affiliated Women and Children Hospital of Jiaxing UniversityJiaxing 314000China Shanghai Key Laboratory of Maternal Fetal Medicine Clinical and Translational Research Center of Shanghai First Maternity and Infant HospitalFrontier Science Center for Stem Cell ResearchSchool of Life Sciences and TechnologyTongji UniversityShanghai 200092China Key Laboratory of Animal Bioengineering and Disease Prevention of Shandong Province College of Animal Science and TechnologyShandong Agricultural UniversityTaian 271018China Women’s Hospital School of MedicineZhejiang UniversityHangzhou 310013China Postgraduate training base Alliance of Wenzhou Medical University Wenzhou Medical UniversityWenzhou 325035China Jiaxing University Master Degree Cultivation Base Zhejiang Chinese Medical UniversityHangzhou 310053China Shanghai Key Laboratory of Maternal Fetal Medicine Shanghai Institute of Maternal-Fetal Medicine and Gynecologic OncologyClinical and Translational Research CenterShanghai First Maternity and Infant HospitalSchool of Life Sciences and TechnologyTongji UniversityShanghai 200092China

Adenomyosis is a poorly understood gynecological disorder lacking effective *** persists regarding“invagination”and“metaplasia”*** endometrial-myometrial junction(EMJ)connects the endometrium and myometrium and is important for diagnosing and classifying adenomyosis,but its in-depth study is just *** single-cell RNA sequencing and spatial profiling,we mapped transcriptional alterations across eutopic endometrium,lesions,and *** lesions,we identified unique epithelial(LGR5+)and invasive stromal(PKIB+)subpopulations,along with WFDC1+progenitor cells,supporting a complex interplay between“invagination”and“metaplasia”theories of ***,we observed endothelial cell heterogeneity and abnormal angiogenic signaling involving vascular endothelial growth factor and angiopoietin ***-cell communication differed markedly between ectopic and eutopic endometrium,with aberrant signaling in lesions involving pleiotrophin,TWEAK,and WNT *** study reveals unique stem cell-like and invasive cell subpopulations within adenomyosis lesions identified,dysfunctional signaling,and EMJ abnormalities critical to developing precise diagnostic and therapeutic strategies.

关键词： adenomyosis single-cell RNA sequencing spatial transcriptomics endometrial-myometrial junction progenitor cells

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An inulin-type fructan CP-A from Codonopsis pilosula attenuates experimental colitis in mice by promoting autophagy-mediated inactivation of NLRP3 inflammasome

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Chinese Journal of Natural Medicines 2024年第03期 249-264页

作者： ZHOU Jiangtao WANG Jun WANG Jiajing LI Deyun HOU Jing LI Jiankuan bai Yun'e gao Jianping School of Pharmaceutical Science Shanxi Medical University

Inulin-type fructan CP-A, a predominant polysaccharide in Codonopsis pilosula, demonstrates regulatory effects on immune activity and anti-inflammation. The efficacy of CP-A in treating ulcerative colitis(UC) is, however, not well-established. This study employed an in vitro lipopolysaccharide(LPS)-induced colonic epithelial cell model(NCM460) and an in vivo dextran sulfate sodium(DSS)-induced colitis mouse model to explore CP-A's protective effects against experimental colitis and its underlying mechanisms. We monitored the clinical symptoms in mice using various parameters: body weight, disease activity index(DAI), colon length, spleen weight, and histopathological scores. Additionally, molecular markers were assessed through enzyme-linked immunosorbent assay(ELISA), quantitative real-time polymerase chain reaction(qRT-PCR), immunofluorescence(IF), immunohistochemistry(IHC), and Western blotting assays. Results showed that CP-A significantly reduced reactive oxygen species(ROS), tumor necrosis factor-alpha(TNF-α), and interleukins(IL-6, IL-1β, IL-18) in LPS-induced cells while increasing IL-4 and IL-10 levels and enhancing the expression of Claudin-1, ZO-1, and occludin proteins in NCM460 cells. Correspondingly, in vivo findings revealed that CPA administration markedly improved DAI, reduced colon shortening, and decreased the production of myeloperoxidase(MPO), malondialdehyde(MDA), ROS, IL-1β, IL-18, and NOD-like receptor protein 3(NLRP3) inflammasome-associated genes/proteins in UC mice. CP-A treatment also elevated glutathione(GSH) and superoxide dismutase(SOD) levels, stimulated autophagy(LC3B, P62, Beclin-1, and ATG5), and reinforced Claudin-1 and ZO-1 expression, thereby aiding in intestinal epithelial barrier repair in colitis ***, the inhibition of autophagy via chloroquine(CQ) diminished CP-A's protective impact against colitis in vivo. These findings elucidate that CP-A's therapeutic effect on experimental colitis possibly involves mitigating i

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Exploring the material basis and mechanism of Moringa oleifera in alleviating slow transit constipation based on network pharmacology and animal models

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Food Science and Human Wellness 2024年

作者： Sijin Li Xiaoyu gao Kaifeng Guo Shuangfeng Liu Weiqian Yang Yan Zhao Jun Sheng Zhongbin bai Yang Tian College of Food Science and Technology， Yunnan Agricultural University Yunnan Key Laboratory of Precision Nutrition and Personalized Food Manufacturing， Yunnan Agricultural University Department of Science and Technology， Yunnan Agricultural University College of Veterinary Medicine， Yunnan Agricultural University Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources， Ministry of Education， Yunnan Agricultural University

Moringa oleifera(M. oleifera) have laxative effects， but their active compositions and mechanisms are not very clear thus far. To this end， we systematically explored the active components and mechanism of M. oleifera leaves in relieving constipation by using the slow transit constipation (STC) mouse model and network pharmacology. The results of animal experiments showed that M. oleifera aqueous extract (MOA) had good laxative activity， and its 70% alcohol soluble part (ASP) also showed significant laxative activity(P < 0.01). Network pharmacological prediction results suggested that L-phenylalanine (Phe) was the key compound of ASP， and it might relieve constipation through tachykinin receptor 1 (TACR1) and three kinds of adrenergic receptors， including alpha-1a (ADRA1A)， alpha-2a (ADRA2A)， and alpha-2b (ADRA2B). Further animal experiment results showed that Phe significantly promoted gastrointestinal motility. Phe may relieve STC by enhancing the release of substance P (SP) and upregulating the mRNA expression of TACR1 in the ileum. Importantly， Phe may also promote intestinal movement by downregulating ADRA2A and ADRA2B and upregulating calmodulin and the mRNA and protein expression of myosin light chain 9 (MYL9) in the ileum， thereby activating the GPCR-MLC signaling pathway. These results lay a foundation for the application of M. oleifera and Phe in constipation.

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农村医疗保障法律制度的比较研究

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西北农林科技大学学报（社会科学版） 2012年第1期12卷 126-130页

作者：柏高原王琳天津医科大学医学人文学院天津300070

要建设和谐社会,必须重视占我国人口70%以上的农民的医疗保障制度建设。通过对国际上具有典型意义国家的农村医疗保障制度立法情况的分析,研究发现国外发达国家和发展中国家在农村医疗保障法律制度方面具有一些共同特点:均建立了完善的... 详细信息

要建设和谐社会,必须重视占我国人口70%以上的农民的医疗保障制度建设。通过对国际上具有典型意义国家的农村医疗保障制度立法情况的分析,研究发现国外发达国家和发展中国家在农村医疗保障法律制度方面具有一些共同特点:均建立了完善的法律体系;同时发挥政府和市场机制在农村医疗保障制度建设中的作用;将强制参保作为农村医疗保障制度的基本原则,同时保证医疗保障制度能够普遍性覆盖全部农民等。我国在农村医疗保障立法方面较为滞后,现行法律体系层次低,且在实施和监督方面存在立法缺陷。因此,我国应借鉴其他国家的经验,建立健全农村医疗保障法律制度,坚持公平优先的立法原则并强化政府在建立农村医疗保障制度中的作用。

关键词：农村医疗保障法律制度国际比较

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中外公务员医疗保障法律制度的比较研究

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医学与哲学（A） 2011年第7期32卷 67-68,74页

作者：柏高原王琳天津医科大学天津300070

许多国家和地区对公务员提供比较专门的医疗保障待遇,如西班牙、韩国和日本。我国公务员在享受基本医疗保险以外,为了确保公务员医疗保障待遇不降低,我国建立了公务员医疗补助制度。通过对各国公务员医疗保障制度立法、保障经费来源及... 详细信息

许多国家和地区对公务员提供比较专门的医疗保障待遇,如西班牙、韩国和日本。我国公务员在享受基本医疗保险以外,为了确保公务员医疗保障待遇不降低,我国建立了公务员医疗补助制度。通过对各国公务员医疗保障制度立法、保障经费来源及保障水平的比较,对我国完善公务员医疗保障制度提出了建议。

关键词：公务员医疗保障法律制度比较研究

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基于蓄能发光多功能材料的公路隧道应急逃生照明研究

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公路交通科技 2017年第5期34卷 102-108页

作者：冯守中李洁白杲武汉广益交通科技股份有限公司湖北武汉430079 安徽中益新材料科技有限公司院士工作站安徽滁州239500

为保证公路隧道的有效节能和防灾应急照明,利用蓄能发光多功能材料涂装了隧道壁面。通过室内模型试验和工程实测,研究了蓄能发光多功能涂料在公路隧道中的辅助增光和应急引导照明性能。结果表明:在公路隧道两侧墙面涂装蓄能发光多功能材... 详细信息

为保证公路隧道的有效节能和防灾应急照明,利用蓄能发光多功能材料涂装了隧道壁面。通过室内模型试验和工程实测,研究了蓄能发光多功能涂料在公路隧道中的辅助增光和应急引导照明性能。结果表明:在公路隧道两侧墙面涂装蓄能发光多功能材料,不仅可以增光增亮和有效减少不同照明灯具的照明功率,还可以明显消除隧道进出口的"黑洞"、"盲光"现象,提高隧道内照明均匀度,消除隧道路面和墙面的光斑现象,有利于行车安全;有助于提高人眼视觉功效,增加小物体可视距离,在紧急情况下可提高司驾人员大于1.3 s以上的安全处置储备时间;蓄能发光多功能材料具有较强的烟雾穿透能力,在隧道墙面涂装可达到黑暗和高烟雾浓度环境下的应急逃生诱导指示照明效果;蓄能发光多功能材料涂装隧道壁面在美观装饰的同时,既防火阻燃、耐污染,又达到了应急逃生照明和节能的作用,克服了传统的逃生指示装置在火灾时的引导效果缺陷。

关键词：隧道工程蓄能发光多功能材料涂装应急逃生穿透烟雾

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中国高铁的集成创新为何能够成功

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学术前沿 2016年第10期 78-88页

作者：高柏西南交通大学中国高铁发展战略研究中心

中国从上世纪90年代下半期开始自主研发以中华之星为代表的高速列车,并在短短的12年里走过从吸收消化再创新,系统集成创新到原始创新的道路。改革开放以来中国有很多产业实施市场换技术的战略,为什么只有高铁取得了突出的成绩?这不仅是... 详细信息

中国从上世纪90年代下半期开始自主研发以中华之星为代表的高速列车,并在短短的12年里走过从吸收消化再创新,系统集成创新到原始创新的道路。改革开放以来中国有很多产业实施市场换技术的战略,为什么只有高铁取得了突出的成绩?这不仅是技术攻关,更是一次资源分配和制度创新,特别是以项目为契机,充分发挥政策的导向和指引作用,大力促进产学研深度融合,注重科研院校在重大项目立项、论证、攻关等核心环节的作用,这些经验都对其它重大项目攻关有着重大的借鉴作用。

关键词：中国高铁创新体系产业政策寡占竞争耦合与系统集成能力产学研深度合作

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Efficacy and safety of PD-1 blockade plus long-course chemoradiotherapy in locally advanced rectal cancer(NECTAR):a multi-center phase 2 study

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Signal Transduction and Targeted Therapy 2024年第4期9卷 1721-1729页

作者： Zhengyang Yang Jiale gao Jianyong Zheng Jiagang Han Ang Li Gang Liu Yi Sun Jie Zhang Guangyong Chen Rui Xu Xiao Zhang Yishan Liu Zhigang bai Wei Deng Wei He Hongwei Yao Zhongtao Zhang Department of General Surgery Beijing Friendship HospitalCapital Medical UniversityState Key Lab of Digestive HealthNational Clinical Research Center for Digestive DiseasesBeijingChina Department of Gastrointestinal Surgery The First Affiliated Hospital of Air Force Medical UniversityXi'anChina Department of General Surgery Beijing Chaoyang HospitalCapital Medical UniversityBeijingChina Department of General Surgery Beijing Xuanwu HospitalCapital Medical UniversityBeijingChina Department of General Surgery Tianjin Medical University General HospitalTianjinChina Department of Anorectal Tianjin People's HospitalTianjinChina Department of Radiology Beijing Friendship Hospital Capital Medical UniversityBeijingChina Department of Pathology Beijing Friendship HospitalCapital Medical UniversityBeijingChina Department of Thoracic Surgery/Institute of Thoracic Oncology West China HospitalSichuan UniversityChengduChina

Adding PD-1 blockade in the neoadjuvant regimens for locally advanced rectal cancer(LARC)patients with microsatellite stable(MsS)/mismatch repair-proficient(pMMR)tumors is an attractive,but debatable *** phase 2,multicenter,prospective,single-arm study enrolled patients from 6 centers from June 2021 to November *** advanced rectal cancer(LARC,cT_(3-4a)N_(0)M_(0) and cT_(1-4a)N_(1-2)M_(0))patients aged≥18 years with the distance from distal border of tumor to anal verge≤10 cm(identified by Magnetic Resonance Imaging)were qualifed for *** patients received long-course radiotherapy(50 Gy/25 fractions,2 Gy/fraction,5 days/week)and three 21-day cycles capecitabine(850-1000 mg/m2,bid,po,day1-14)and three 21-day cycles tislelizumab(200 mg,***,day8)as *** mesorectal excision(TME)was 6-12 weeks after the end of radiotherapy to achieve radical resection.A total of 50 patients were enrolled in this *** pathological complete response rate was 40.0%[20/50,95%confidence interval(CI):27.61-53.82%],while 15(30.0%,95%CI:19.1-43.75%),9(18.0%,95%CI:9.77-30.8%),2(4.0%,95%CI:1.10-13.46%)patients respectively achieved grade 1,2,and 3 tumor ***-related adverse events(TRAEs)occurred in 28(56.0%)LARC patients,including 26(52.0%)with grade I-II and 2(4.0%)with grade II(1 with grade 3 immune-related colitis and 1 with grade 3 rash).PD-1 blockade plus long-course chemoradiotherapy(CRT)showed promising therapeutic effects according to pathological complete response rate and is well-tolerated in LARC patients.A larger randomized controlled study is desired to further validate the above findings.

关键词： rectal cancer phase

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Genetic and pathogenic characterization of new infectious bronchitis virus strains in the GVI-1 and GI-19 lineages isolated in central China

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Journal of Integrative Agriculture 2024年第7期23卷 2407-2420页

作者： Yuhan Yang Dou Wang Yaning bai Wenyan Huang Shimin gao Xingchen Wu Ying Wang Jianle Ren Jinxin He Lin Jin Mingming Hu Zhiwei Wang Zhongbing Wang Haili Ma Junping Li Libin Liang College of Veterinary Medicine Shanxi Agricultural UniversityJinzhong 030801China Shanxi Provincial Animal Disease Prevention and Control Center Taiyuan 030000China

Avian infectious bronchitis(IB)is a highly contagious infectious disease caused by infectious bronchitis virus(IBV),which is prevalent in many countries worldwide and causes serious harm to the poultry *** present,many commercial IBV vaccines have been used for the prevention and control of IB;however,IB outbreaks occur *** this study,two new strains of IBV,SX/2106 and SX/2204,were isolated from two flocks which were immunized with IBV H120 vaccine in central *** and recombination analysis indicated that SX/2106,which was clustered into the GI-19 lineage,may be derived from recombination events of the GI-19 and GI-7 strains and the LDT3-A *** analysis showed that SX/2204 belongs to the GVI-1 lineage,which may have originated from the recombination of the GI-13 and GVI-1 strains and the H120 *** virus cross-neutralization test showed that the antigenicity of SX/2106 and SX/2204 was different from *** experiments found that both SX/2106 and SX/2204 could replicate effectively in the lungs and kidneys of chickens and cause disease and death,and H120 immunization could not provide effective protection against the two IBV *** is noteworthy that the pathogenicity of SX/2204 has significantly increased compared to the GVI-1 strains isolated previously,with a mortality rate up to 60%.Considering the continuous mutation and recombination of the IBV genome to produce new variant strains,it is important to continuously monitor epidemic strains and develop new vaccines for the prevention and control of IBV epidemics.

关键词： infectious bronchitis virus GI-19 lineage GVI-1 lineage complete genome recombination pathogenicity

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汽车油泥模型中线与面关系研究

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机械设计 2015年第4期32卷 126-128页

作者：高柏多杨雄勇湖南大学设计艺术学院湖南长沙410012

为使设计师与模型师在汽车油泥模型阶段能够高效地沟通与配合,提出建立一种以线与面的关系为基础的设计新模式。通过对传统与现代两种汽车油泥模型制作方法进行分析与对比,结合具体的设计案例,对汽车油泥模型制作过程中的线与面关系进... 详细信息

为使设计师与模型师在汽车油泥模型阶段能够高效地沟通与配合,提出建立一种以线与面的关系为基础的设计新模式。通过对传统与现代两种汽车油泥模型制作方法进行分析与对比,结合具体的设计案例,对汽车油泥模型制作过程中的线与面关系进行分类与探究。得出线与面的关系在汽车油泥模型阶段的3种表现形式,并综合这3种表现形式,构建出新的设计协作模式,以提高汽车开发的效率。

关键词：汽车造型设计油泥模型线面

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