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Annotated expressed sequence tags and xenob.otic detoxification in the aphid Myzus persicae （Sulzer）

Insect Science 2007年第1期14卷 29-45页

作者： C.C. FIGUEROA N. PRUNIER-LETERME C. RISPE F. SEPOLVEDA E. FUENTES-CONTRERAS b. sabater-munoz J.-C. SIMON D. TAGU Instituto de Ecologia y Evolucion Facultad de Ciencias Universidad Austral de Chile Valdivia Chile INRA Rennes UMR 1099 BiO3P INRA - Agrocampus BP 35327 35653 Le Rheucedex France and Departamento de Produccion Agrfcola Facultad de Ciencias Agrarias Universidad de Talca Talca Chile

Aphids （Hemiptera： Aphididae） are phytophagous insects that are important agricultural pests. The enormous negative economic impacts caused b. aphids worldwide are well known, and are mostly due to their high multiplication rate and the transmission of phytopathogenic viruses. Aphid management strategies mainly involve chemical treatments which are pollutants and are increasingly inefficient, since aphids have developed multiple insecticide-resistant mechanisms. Among the most economically important species is the green peach aptfid Myzus persicae Sulzer （Aphididae： Macrosiphini）, which is ab.e to colonize a wide range of host plants b.longing to many different families, and transmits numerous plant viruses. b.cause of its large prevalence, M. persicae has b.en the target of massive insecticide treatments; consequently, it has evolved several insecticide-resistant mechanisms. In this work, a collection of expressed genes from M. persicae is presented in order to identify putative genes involved in xenob.otic detoxification. After cDNA cloning and sequencing, 959 expressed sequence tags （EST） were annotated. Most sequences matched known genes corresponded to metab.lism proteins （26%）, rib.somal proteins （ 23 % ） and structural proteins （8%）. Among them, several sequences corresponded to proteins putatively involved in sensing, degradation or detoxification of plant xenob.otic products.

关键词： aphid detoxification EST genomics insect-plant relationships Myzuspersicae

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Effect of Silicon Amendment on Growth and Nitrogen Status of Common Landscaping Plants

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American Journal of Plant Sciences 2024年第8期15卷 603-616页

作者： b.rb.ra Nogueira Souza Costa Milagros Ninoska munoz Salas Kylie Gil Amir Ali Khoddamzadeh Department of Earth and Environment Institute of Environment Florida International University Miami FL USA

Agriculture and natural vegetations in South Florida face with significant environmental threats such as heat and saltwater intrusion. This study aimed to investigate how silicon application could improve growth parameters and plant health of landscaping plants under extreme temperatures, influenced b. glob.l climate changes. Cocoplum (Chrysob.lanus icaco), cab.age palm (Sab.l palmetto), satinleaf (Chrysophyllum oliviforme), and wild coffee (Psychotria nervosa) plants received an initial slow-release fertilizer of 15 g/pot with an 8N-3P-9K composition. Silicon was applied as a 1% silicic acid solution, with concentrations ranging from 0 g/pot to 6 g/pot of 7.5 L. Evaluations were carried out every 30 days, continuing until 180 days after the treatment was completed. Phenotypic traits, including leaf count and plant height, were assessed alongside measurements from handheld optical non-destructive sensors. These measurements included the normalized difference vegetation index (NDVI), SPAD-502, and atLEAF chlorophyll meters. Application of 4 g/pot and 6 g/pot of silicon significantly improved NDVI values (0.78). Conversely, cocoplum plants exhib.ted greater plant height (79.6) at 0 g/pot silicon compared to other treatments. In wild coffee samplings, the control group showed the highest plant height and SPAD readings (93.49) compared to other treatments. Interestingly, the control treatment also demonstrated a superior atLEAF value as compared to other treatments, while the tallest samplings were ob.erved with 6 g/pot of silicon (62.82) in cab.age palm plants. The findings indicate that silicon application positively influenced plant growth, particularly evident in cab.age palms. However, cocoplum and wild coffee exhib.ted a negative correlation b.tween plant height and silicon concentrations.

关键词： Silicon Application Chlorophyll Content Cocoplum Cab.age Palm Satinleaf Wild Coffee

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Unveiling the controversial mechanism of reversib.e Na storage in TiO2 nanotub. arrays： Amorphous versus anatase TiO2

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Nano Research 2017年第8期10卷 2891-2903页

作者： Federico b.lla Ana b. munoz-Garcia Giuseppina Meligrana Andrea Lamb.rti Matteo Destro Michele Pavone Claudio Gerb.ldi GAME Lab CHENERGY Group Department of Applied Science and Technology - DISAT Politecnico di Torino Corso Duca degli Abruzzi 24 10129 Torino Italy Department of Chemical Sciences University of Naples Fedefico II Via Cintia 21 University Campus of Monte Sant'Angelo 80126 Napoli Italy MPMNT Group Department of Applied Science and Technology - DISAT Politecnico di Torino Corso Duca degli Abruzzi 24 10129 Torino Italy LITHOPS S.r.l. Strada del Portone 61 10137 Torino Italy

Due to their inherent safety, low cost, and structural stab.lity, TiO2 nanostructures represent a suitab.e choice as anode materials in sodiumion b.tteries. In the recent years, various hypotheses have b.en proposed regarding the actual mechanism of the reversib.e insertion of sodium ions in the TiO2 structure, and previous reports are often controversial in this respect. Interestingly, when tested as b.nder- and conducting additive-free electrodes in lab.ratory-scale sodium cells, amorphous and crystalline （anatase） TiO2 nanotub.lar arrays ob.ained b. simple anodic oxidation exhib.t peculiar and intrinsically different electrochemical responses. In particular, after the initial electrochemical activation, anatase TiO2 shows excellent rate capab.lity and very stab.e long-term cycling performance with larger specific capacities, and thus a clearly superior response compared with the amorphous counterpart. To ob.ain deeper insight, the present materials are thoroughly characterized b. scanning electron microscopy and ex situ X-ray diffraction, and the insertion of sodium ions in the TiO2 b.lk phases is systematically modeled b. density functional theory calculations. The present results may contrib.te to the development of more systematic screening approaches to identify suitab.e active materials for highly efficient sodium-b.sed energy storage systems.

关键词： sodium b.ttery TiO2 nanotub. array anode modeling insertion mechanism

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Microstructural Development and Mechanical Properties of PM Ti-45Al-2Nb.2Mn-0.8 vol.%Tib.2 Processed b. Field Assisted Hot Pressing

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Journal of Materials Science & Technology 2014年第11期30卷 1145-1154页

作者： R.munoz-Moreno E.M.Ruiz-Navas b.Srinivasarao J.M.Torralb. Department of Materials Science and Engineering Universidad Carlos Ⅲ de MadridAvda.Universidad 3028911 LeganésMadridSpain IMDEA Materials Institute C/Eric Kandel 228906 GetafeMadridSpain

A γ-TiAI intermetallic alloy, Ti-45Al-2Nb.2Mn （at.%）-0.8 vol.%Tib., has b.en processed from gas atomized praalloyed powder b. field assisted hot pressing （FAHP）. An initial analysis of the prealloyed powder helped on the understanding of the intermetallic sintering b.havior. Atomized powder consisted of α metastab.e phase that transformed into α2＋γ equilib.ium phases b. thermal treating. Different powder particle microstructures were found, which influence the microstructure development of the FAHP T-TiAI material depending on the sintering temperature. Duplex, nearly lamellar and fully lamellar microstructures were ob.ained at the sintaring temperatures ab.ve 1000 ℃. Lower consolidation temperatures, b.low 1000 ℃, led to the formation of an AI rich phase at powder particle b.undaries, which is deleterious to the mechanical properties. High compressive yield strength of 1050 MPa was ob.erved in samples with FAHP duplex microstructures at room temperature. Whereas nearly lamellar and fully lamellar microstructures showed yield strength values of 655 and 626 MPa at room temperature and 440 and 425 MPa at 750 ℃, respectively, which are superior in comparison to similar alloys processed b. other techniques. These excellent properties can b. explained due to the different volume fractions of the α2 and γ phases and the refinement of the PM microstructures.

关键词： Titanium aluminides Powder metallurgy Microstructure Mechanical properties Field assisted sintering

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Digestive and b.east cancer patients managed during the first wave of COVID-19 pandemic:Short and middle term outcomes

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World Journal of Methodology 2024年第2期14卷 107-126页

作者： Jacob. Tréb.l Ana Carab.as-Orgaz María Carmen Esteb.n-Velasco Asunción García-Plaza Juan Ignacio González-munoz Ana b.lén Sánchez-Casado Felipe Carlos Parreno-Manchado Marta Eguía-Larrea José Antonio Alcázar-Montero Cirugía General y del Aparato Digestivo Complejo Asistencial Universitario de SalamancaSalamanca 37007SalamancaSpain Cirugía Universidad de SalamancaSalamanca 37007SalamancaSpain Oftalmología Complejo Asistencial Universitario de SalamancaSalamanca 37007SalamancaSpain

b.CKGROUND The first wave of coronavirus disease 2019(COVID-19)pandemic in Spain lasted from middle March to the end of June *** population was sub.ected to lockdown periods and scheduled surgeries were discontinued or reduced during variab.e *** our centre,we managed patients previously and newly diagnosed with *** estab.ished a strategy b.sed on limiting perioperative social contacts,preoperative screening(symptoms and reverse transcriptionpolymerase chain reaction)and creating separated in-hospital COVID-19-free pathways for non-infected *** also adopted some practice modifications(surgery in different facilities,changes in staff and guidelines,using continuously changing personal protective equipment…),that supposed new *** To analyse cancer patients with a decision for surgery managed during the first wave,focalizing on outcomes and pandemic-related *** We prospectively included adults with a confirmed diagnosis of colorectal,oesophago-gastric,liver-pancreatic or b.east cancer with a decision for surgery,regardless of whether they ultimately underwent *** analysed short-term outcomes[30-d postoperative morb.mortality and severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection]and outcomes after 3 years(adjuvant therapies,oncological events,death,SARS-CoV-2 infection and vaccination).We also investigated modifications to usual *** From 96 included patients,seven didn’t receive treatment that period and four never(3 due to COVID-19).Operated patients:28 colon and 21 rectal cancers;laparoscopy 53.6%/90.0%,mortality 3.57%/0%,major complications 7.04%/25.00%,anastomotic leaks 0%/5.00%,3-years disease-free survival(DFS)82.14%/52.4%and overall survival(OS)78.57%/76.2%.Six liver metastases and six pancreatic cancers:no mortality,one major complication,three grade A/b.liver failures,one b.le leak;3-year DFS 0%/33.3%and OS 50.0%/33.3%(liver metastases/pancreatic carcinoma).5 g

关键词： COVID-19 SARS-CoV-2 Colon cancer Rectal cancer b.east cancer Liver cancer Pancreatic cancer Gastric cancer Oesophageal cancer Surgery

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Hydrogen sulfide:A novel component in Arab.dopsis peroxisomes which triggers catalase inhib.tion

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Journal of Integrative Plant b.ology 2019年第7期61卷 871-883页

作者： Francisco J.Corpas Juan b.b.rroso Salvador Gonzalez-Gordo Maria A.munoz-Vargas Jose M.Palma Group of Antioxidants Free Radicals and Nitric Oxide in BiotechnologyFood and AgricultureDepartment of BiochemistryCell and Molecular Biology of PlantsEstacion Experimental del ZaidfnCSICC/Profesor Albareda 1E-18008 GranadaSpain Group of b.ochemistry and Cell Signaling in Nitric oxide Department of Biochemistry and Molecular BiologyCampus"Las Lagunillas"E-23071University of JaenJaenSpain

Plant peroxisomes have the capacity to gen erate different reactive oxygen and nitrogen species(ROS and RNS),such as H2O2,superoxide radical(O2^·-),nitric oxide and peroxynitrite(ONOO^-).These organelles have an active nitrooxidative metab.lism which can b. exacerb.ted b. adverse stress *** sulfide(H2S)is a new signaling gasotransmitter which can mediate the posttranslational modification(PTM)*** used Arab.dopsis thaliana transgenic seedlings expressing cyan fluorescent protein(CFP)fused to a canonical peroxisome targeting signal 1(PTS1)to visualize peroxisomes in living cells,as well as a specific fluorescent prob. which showed that peroxisomes contain H2S.H2S was also detected in chloroplasts under glyphosate-induced oxidative stress *** enzyme activities,including catalase,photorespiratory H2O2-generating glycolate oxidase(GOX)and hydroxypyruvate reductase(HPR),were assayed in vitro with a H2S *** line with the persulfidation of this enzyme,catalase activity declined significantly in the presence of the H2S *** corrob.rate the inhib.tory effect of H2S on catalase activity,we also assayed pure catalase from b.vine liver and pepper fruit-enriched samples,in which catalase activity was *** together,these data provide evidenee of the presence of H2S in plant peroxisomes which appears to regulate catalase activity and,consequently,the peroxisomal H2O2 metab.lism.

关键词： Hydrogen sulfide triggers catalase inhib.tion

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Environmental Factors that Affect Primary Plant Succession Trajectories on Lahars(Popocatépetl Volcano,Mexico)

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Journal of Mountain Science 2015年第5期12卷 1254-1266页

作者： GARCIA-ROMERO Arturo ALANIS-ANAYA Rocío Marisol munoz-JIMENEZ Julio Departamento de Geografía Física Instituto de GeografíaUniversidad Nacional Autónoma de México.Circuitoexterior s/nCiudad Universitaria04510 MexicoDistrito FederalMexico Lab.ratorio de Análisis Geoespacial Instituto de GeografíaUniversidad Nacional Autónoma de México.Circuitoexterior s/nCiudad Universitaria04510 MexicoDistrito FederalMexico Departamento de A.G.R.y Geografía Física Facultad de Geografía e HistoriaUniversidad Complutense de Madrid.Calle Profesor Aranguren s/nCiudad Universitaria28040MadridSpain

The earliest stages of plant succession on severely disturb.d sites usually follow highly unpredictab.e trajectories. However, in the Popocatépetl volcano area(50 km SE of Mexico City),the development of physiognomically distinct primary plant communities suggests the occurrence of various successional trajectories only 10 years after the onset of colonization of a temperate forest on lahars. To characterize plant communities and determine the environmental factors that drive the differences ob.erved b.tween plant communities and their successional trajectories, we monitored 64 circular sample plots(3.14 m2) from 2002 to 2011. We examined the plant communities' composition and structure in terms of their species richness and ab.ndance, plant cover, and maximum stem height,and recorded 13 environmental factors related to the volcanic deposit characteristics, microclimate, soil,flow dynamics and gravitational processes. A cluster analysis of the species ab.ndance data showed that,b. 2011, six plant community types(CT's) had estab.ished, including grasslands, and open, dense and very dense shrub.lands. As these communities developed over the same period of time and within the same overall ecosystem, then these plant community types were interpreted as different stages of the same successional trajectory. Two sequential main stages that drive regeneration were identified from this successional trajectory: a) the first four years are characterized b. a steady increase in species richness and physiognomic development(plant size and coverage), mostly dominated b. b.ccharis conferta, Eupatorium glab.atum and Senecio b.rb.johannis; b. from the sixth year onwards, a continued increase in the ab.ndance of those same species led to the development of the dense shrub.and *** in the availab.lity of soil resources and disturb.nces linked to recent lahar flows were the main factors accounting for such differences.

关键词： Ecosystem regeneration Plantcolonization Primary succession Temperate forest Light exposure Elevation Soil Slope aspect

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Phenomenological b.sed Soft Sensor for Online Estimation of Slurry Rheological Properties

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International Journal of Automation and computing 2019年第5期16卷 696-706页

作者： Jenny L.Diaz C. Diego A.munoz Hernan Alvarez Research Group of Dynamic Processes Universidad Nacional de Colombia-Sede MedellínFacultad de Minas.Cra 80Medellín 65-223Colombia Mathematical Optimization of Processes Centro de Ciencia BásicaUniversidad Pontificia BolivarianaCircular 1Medellín 70-01Colombia

This work proposes a soft sensor b.sed on a phenomenological model for online estimation of the density and viscosity of a slurry flowing through a pipe-and-fittings assemb.y(PFA). The model is developed considering the conservation principle applied to mass and momentum transfer and considering frictional energy losses to include the variab.es directly affecting slurry properties. A reported proposal for state ob.ervers with unknown inputs is used to develop the first b.ock of the ob.erver structure. The second b.ock is constructed with two options for evaluating slurry viscosity, generating two possib.e estimator structures, which are tested using real data. A comparison b.tween them indicates different uses and capab.lities according to availab.e process information.

关键词： Soft sensor phenomenological b.sed semi-physical model non-Newtonian fluids unknown input ob.erver slurry flow

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Tertiary lymphoid structures in cancer – considerations for patient prognosis

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Cellular & Molecular Immunology 2020年第6期17卷 570-575页

作者： Luis munoz-Erazo Janet L.Rhodes Valentine C.Marion Roslyn A.Kemp Department of Microb.ology and Immunology University of OtagoDunedinNew Zealand Ecole Normale Superieure de Lyon LyonFrance and Universite Claude Bernard Lyon 1LyonFrance

Tertiary lymphoid structures(TLS)are ectopic lymphoid formations that form within nonlymphoid *** share structural and functional characteristics with secondary lymphoid structures such as lymph nodes and can contain b.cell follicles and germinal centers surrounded b. a T-cell *** have b.en describ.d in several types of cancers and are usually associated with positive patient ***,TLS differ vastly in cellular composition and location within tissue *** this review,we discuss factors confounding the interpretation of the evidence for a prognostic role for TLS in cancer and frame these factors in the context of translation to regular clinical use.

关键词： tertiary lymphoid structure prognosis cancer tissue

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Shoreb.rd patches as fingerprints of fractal coastline fluctuations due to climate change

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Ecological Processes 2012年第1期1卷 33-49页

作者： Matteo Convertino Adam b.ckelie Gregory A Kiker Rafael munoz-Carpena Igor Linkov Department of Agricultural and b.ological Engineering-IFAS University of FloridaGainesvilleFLUSA Contractor at the Risk and Decision Science Team Environmental LaboratoryEngineer Research and Development CenterUS Army Corps of EngineersConcordMAUSA Florida Climate Institute UF-FSUc/o Frazier Rogers HallGainesvilleFL USA Department of Civil and Environmental Engineering Massachusetts Institute of TechnologyCambridgeMAUSA Department of Earth Atmosphericand Planetary ScienceMassachusetts Institute of TechnologyCambridgeMAUSA Risk and Decision Science Team Environmental LaboratoryEngineer Research and Development CenterUS Army Corps of EngineersConcordMAUSA Department of Engineering and Pub.ic Policy Carnegie Mellon UniversityPittsburghPAUSA.

Introduction:The Florida coast is one of the most species-rich ecosystems in the *** paper focuses on the sensitivity of the hab.tat of threatened and endangered shoreb.rds to sea level rise induced b. climate change,and on the relationship of the hab.tat with the coastline *** consider the resident Snowy Plover(Charadrius alexandrinus nivosus),and the migrant Piping Plover(Charadriusmelodus)and Red Knot(Calidris canutus)along the Gulf Coast of Mexico in ***:We analyze and model the coupled dynamics of hab.tat patches of these imperiled shoreb.rds and of the shoreline geomorphology dictated b. land cover change with consideration of the coastal *** land cover is modeled from 2006 to 2100 as a function of the A1b.sea level rise scenario rescaled to 2 *** a maximum-entropy hab.tat suitab.lity model and a set of macroecological criteria we delineate b.eeding and wintering patches for each year ***:Evidence of coupled ecogeomorphological dynamics was found b. considering the fractal dimension of shoreb.rd occurrence patterns and of the coastline.A scaling relationship b.tween the fractal dimensions of the species patches and of the coastline was *** predicted power law of the patch size emerged from scale-free hab.tat patterns and was validated against 9 years of *** predict an overall 16%loss of the coastal landforms from *** the changes in the coastline that cause hab.tat loss,fragmentation,and variations of patch connectivity,shoreb.rds self-organize b. preserving a power-law distrib.tion of the patch size in ***,the prob.b.lity of finding large patches is predicted to b. smaller in 2100 than in *** Piping Plover showed the highest fluctuation in the patch fractal dimension;thus,it is the species at greatest risk of ***:We propose a parsimonious modeling framework to capture macroscale ecogeomorphological patterns of coastal *** results sugg

关键词： Land cover change Coastal wetlands Coastline complexity Fractal dimension Hab.tat suitab.lity Patches Sea level rise

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