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Lo.alised so.id-state nano.o.e fab.icatio. via co.tro.led b.eakdo.n using o.-chip electro.es

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Nano.Research 2022年第11期15卷 9881-9889页

作者： Jasper P.Fried Jaco.0}L.Swett b.no. Paulo.e Nadappuram Aleksandra Fedo.yuk Alex Gee o.drej E.Dyck James R.Yates Aleksandar P.Ivano. Jo.hua b.edel.Jan A.Mo. Department o. Materials University of OxfordOxfordOX13PHUK Scho.l o. Chemistry University of New South WalesSydneyNew South Wales 2052Australia Department o. Chemistry Imperial College LondonLondonW120BZUK Center fo. Nano.hase Materials Sciences Oak Ridge National LaboratoryOak RidgeTN 37830USA Instituto.de Tecno.o.ia Química e b.o.ógica António.Xavier Universidade Nova de LisboaAv.da RepúblicaOeiras 2780-157Portugal Scho.l o. Physics and Astro.o.y Queen Mary University of LondonLondonE14NSUK

Co.tro.led b.eakdo.n has recently emerged as a highly accessib.e technique to.fab.icate so.id-state ***,in its mo.t co.mo. fo.m,co.tro.led b.eakdo.n creates a single nano.o.e at an arb.trary lo.atio. in the ***,we intro.uce a new strategy whereb. b.eakdo.n is perfo.med b. applying the electric field b.tween an o.-chip electro.e and an electro.yte so.utio. in co.tact with the o.po.ite side o. the *** demo.strate two.advantages o. this ***,we can independently fab.icate multiple nano.o.es at given po.itio.s in the memb.ane b. lo.alising the applied field to.the ***,we can create nano.o.es that are self-aligned with co.plementary nano.lectro.es b. applying vo.tages to.the o.-chip electro.es to.lo.ally heat the memb.ane during co.tro.led *** new co.tro.led b.eakdo.n metho. pro.ides a path to.ards the affo.dab.e,rapid,and auto.atab.e fab.icatio. o. arrays o. nano.o.es self-aligned with co.plementary o.-chip nano.tructures.

关键词： so.id-state nano.o.es dielectric b.eakdo.n nano.ab.icatio. single-mo.ecule sensing nano.o.e arrays

Micro.tructure and mechanical pro.erties o. Ti-6Al-4V-5% hydro.yapatite co.po.ite fab.icated using electro. b.am po.der b.d fusio.

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Jo.rnal o. Materials Science & Techno.o.y 2019年第2期35卷 309-321页

作者： César A.Terrazas Lawrence E.Murr Diego.b.rmudez edel.Arrieta David A.Ro.0}rso. Ryan b.Wicker W.M.Keck Center fo. 3D Inno.atio. The University of Texas at El PasoEl PasoTX 79968USA Department o. Mechanical Engineering The University of Texas at El PasoTX 79968USA Department o. Metallurgical Materials and Biomedical EngineeringThe University of Texas at El PasoTX 79968USA

A no.el, Ti-6 Al-4 V(Ti64)/Hydro.yapatite(HA at 5% b. weight co.centratio.) metal/ceramic co.po.ite has b.en fab.icated using electro. b.am po.der b.d fusio.(EPb.) additive manufacturing(AM): specifically, the co.mercial electro. b.am melting(Eb.?) pro.ess. In additio. to.so.id Ti64 and Ti64/5% HA samples, fo.r different unit cell(mo.el) o.en-cellular mesh structures fo. the Ti64/5% HA co.po.ite were fab.icated having densities ranging fro. 0.68 to.1.12 g/cm^3, and co.respo.ding Yo.ng's mo.uli ranging fro. 2.9 to.8.0 GPa, and co.pressive strengths ranging fro. ~3 to.11 MPa. The so.id Ti64/5%HA co.po.ite exhib.ted an o.timal tensile strength o. 123 MPa, and elo.gatio. o. 5.5% in co.trast to.a maximum co.pressive strength o. 875 MPa. b.th the so.id co.po.ite and mesh samples defo.med primarily b. b.ittle defo.matio., with the mesh samples exhib.ting erratic, b.ittle crushing. So.id, EPb.-fab.icated Ti64 samples had a Vickers micro.ndentatio. hardness o. 4.1 GPa while the Ti64/5%HA so.id co.po.ite exhib.ted a Vickers micro.ndentatio. hardness o. 6.8 GPa. The lo.est density Ti64/5%HA co.po.ite mesh strut sectio.s had a Vickers micro.ndentatio. hardness o. 7.1 GPa. o.tical metallo.raphy(o.) and scanning electro. micro.co.y(SEM) analysis sho.ed the HA disperso.ds to.b. highly segregated alo.g do.ain o. grain b.undaries, b.t ho.o.eneo.sly distrib.ted alo.g alpha(hcp) platelet b.undaries within these do.ains in the Ti64 matrix fo. b.th the so.id and mesh co.po.ites. The alpha platelet width varied fro. ~5 μm in the EPb.-fab.icated Ti64 to.~1.1 m fo. the Ti64/5%HA mesh strut. The precurso. HA po.der diameter averaged 5 μm, in co.trast to.the dispersed HA particle diameters in the Ti64/5%HA co.po.ite which averaged 0.5 m. This wo.k highlights the use o. EPb. AM as a no.el pro.ess fo. fab.icatio. o. a true co.po.ite structure, co.sisting o. a Ti64 matrix and interspersed and expo.ed HA do.ains, which to.the autho.s' kno.ledge has no. b.en repo.ted b.fo.e. The results also.illu

关键词： Hydro.yapatite (HA) Metal-matrix ceramic co.po.ites o.en-cellular (mesh) structures Electro.-b.am po.der b.d fusio. (EPb.) Additive manufacturing (AM) Mechanical pro.erties Micro.tructure characterizatio.

Selective Asso.iatio. b.tween Cephalo.po.in, Quino.o.e, Macro.ide, and Penicillin Antib.o.ic Co.sumptio. and Childho.d o.0}sity in Euro.e

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Health 2015年第10期7卷 1306-1314页

作者： Gab.r Ternak Zs edel.Zs Feiszt J. Szekeres b. Visegrady I. Wittmann Faculty o. Medicine Chair of Migration Health University of Pécs Pécs Hungary Faculty o. Medicine Second Department of Internal Medicine and Nephrology Centre University of Pécs Pécs Hungary Faculty o. Medicine First Department of Internal Medicine Department of Infectology University of Pécs Pécs Hungary Faculty o. Medicine Department of Medical Microbiology and Immunology University of Pécs Pécs Hungary Faculty o. Medicine Department of Biophysics University of Pécs Pécs Hungary

The accelerated weight gain in pro.uctive animals as a result o. feeding antib.o.ic enriched fo.der has b.en well kno.n fo. decades. The b.tter energy harvest is the result o. mo.ified gut micro.0}o.a as a co.sequence o. applied antib.o.ics. Similar mechanisms might result o.0}sity in humans as well. o.0}ectives: Finding asso.iatio.s b.tween glo.0}l antib.o.ic co.sumptio. o. different classes in EU co.ntries and o.0}sity data in adults and children pro.e that antib.o.ics might play a significant ro.e in the develo.ment o. o.0}sity “epidemics” and related illnesses. Metho.s: Antib.o.ic co.sumptio. data were co.pared with o.0}sity figures in adults and children in Euro.ean co.ntries and statistically analyzed fo. significance. Results: Significant co.relatio. was fo.nd b.tween the average yearly co.sumptio. o. cephalo.po.ins (p = 0.007), quino.o.es (p = 0.031), macro.ides (p = 0.000083) and childho.d o.0}sity data, b.t no.significant asso.iatio. was o.0}erved with the average penicillin co.sumptio.. No.asso.iatio. was o.0}erved b.tween adult o.0}sity and any o. the antib.o.ic classes studied. Co.clusio.s: o.r results suppo.t the hypo.hesis that different types o. antib.o.ics might influence the develo.ment o. o.0}sity amo.g children, and this finding can serve as a unified explanatio. fo. the develo.ment o. o.0}sity “epidemics”, similarly to.the o.0}sity and gut flo.a alteratio.-related diseases (type 2 diab.tes mellitus, autism, etc.).

关键词： Antib.o.ic Co.sumptio. Cephalo.po.in Quino.o.e Macro.ide Penicillin Childho.d o.0}sity Adult o.0}sity

A New β-Estradio.-lnducib.e Vecto. Set that Facilitates Easy Co.structio. and Efficient Expressio. o. Transgenes Reveals Cb.3-Dependent Cyto.lasm to.To.o.last Translo.atio. o. CIPK5

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Mo.ecular Plant 2013年第6期6卷 1814-1829页

作者： Kathrin Schliicking Kai H. edel.Maria M. Drerupa Philipp Ko.ter Christian Eckert Leo.ie Steinho.st Rainer Waadt o.iver b.tistic Jo.g Kudla Institut fo. b.o.o.ie und b.o.echno.o.ie der Pflanzen Universit~t MQnster Schlossplatz 4 48149 MQnster Gel'many _ . _ Present address: University o. Califo.nia San Diego Division of Biological Sciences Cell and Developmental Biology Section 9500 Gilman Drive #0116 LaJolla CA 92093-0116 USA

Transient and stab.e expressio. o. transgenes is central to.many investigatio.s in plant b.o.o.y research. Chemical regulatio. o. expressio. can circumvent pro.0}ems o. plant lethality caused b. co.stitutive o.erexpressio. o. allo. inducib.e kno.k （o.t/do.n） appro.ches. Several chemically inducib.e o. repressib.e systems have b.en describ.d and successfully applied. Ho.ever, clo.ing and applicatio.-specific mo.ificatio. o. mo.t availab.e inducib.e expressio. systems have b.en limited and remained co.plicated due to.restricted clo.ing o.tio.s. Here we describ. a new set o. 57 vecto.s that enab.e transgene expressio. in transiently o. stab.y transfo.med cells. All vecto.s harb.r a synthetically o.timized XVE expressio. cassette, allo.ing I^-estradio. mediated pro.ein expressio.. Plasmids are equipped with the repo.ter genes GUS, GFP, mCherry, o. with HA and Strepll epito.e tags and harb.r an o.timized multiple clo.ing site fo. flexib.e and simple clo.- ing strategies. Mo.eo.er, the vecto. design allo.s simple sub.titutio. o. the driving pro.o.er to.achieve tissue-specificity o. to.mo.ulate expressio. ranges o. inducib.e transgene expressio.. We repo.t details o. the kinetics and do.e-dependence o. expressio. inductio. in Arab.do.sis leaf meso.hyll pro.o.lasts, transiently transfo.med Nico.iana b.nthamiana leaves, and stab.y transfo.med Arab.do.sis plants. Using these vecto.s, we investigated the influence o. Cb. （Calcineurin b.like） pro.ein expressio. o. the sub.ellular lo.alizatio. o. CIPKs （Calcineurin b.like interacting pro.ein kinases）. These analyses unco.ered that induced co.expressio. o. Cb.3 is fully sufficient fo. dynamic translo.atio. o. CIPK5 fro. the cyto.lasm to.the to.o.last. Thus, the vecto. system presented here facilitates a b.o.d range o. research applicatio.s.

关键词： vecto. inducib.e gene expressio. βestradio. Arab.do.sis Cb. CIPK.

Expo.ure o. Co.mo. b.an Seeds to.Liquid Nitro.en Mo.ifies Mineral Co.po.itio. o. Yo.ng Plantlet Leaves

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American Jo.rnal o. Plant Sciences 2016年第12期7卷 1612-1617页

作者： Inaudis Cejas Annekathrin Rumlo. Ariel Turcio. Flo.ent Engelmann Marco. edel.Martínez Lo.rdes Yab.r Jutta Papenb.o.k Jo.é Carlo. Lo.enzo Lab.rato.y fo. Plant b.eeding Centro de Bioplantas Universidad de Ciego de ávila Ciego de ávila Cuba Leib.iz University Hanno.er Institute of Botany Hannover Germany IRD UMR DIADE Montpellier France

Many pub.icatio.s describ. cryo.reservatio. techniques b.t o.ly a few studies have fo.used o. the b.o.hemical and physio.o.ical changes o.curring in plants regenerated fro. seeds expo.ed to.liquid nitro.en. This paper aims at describ.ng the effect o. co.mo. b.an seed cryo.to.age o. mineral nutritio. o. yo.ng plantlets. The fo.lo.ing elements were measured o. leaves o. 10-day-o.d plantlets fro. no.-cryo.reserved and cryo.reserved seeds: Al, b. b., Ca, Cd, Co. Cr, Cu, Fe, K, Mg, Mn, Na, Ni, P, S, Se, Sr and Zn. At 10 days after so.ing, b.th treatments (co.tro. and cryo.reserved seeds) sho.ed 100% seed germinatio. witho.t any visual pheno.ypic difference. Ho.ever, co.tents o. several elements in the leaves were different. Expo.ure o. seeds to.liquid nitro.en decreased Cu, Cd and Na uptake and increased ab.o.ptio. o. b.and Al. Further studies are required to.understand the mechanisms underlying the relatio.ship b.tween seed expo.ure to.liquid nitro.en and mineral nutritio. during the early stages o. plantlet gro.th.

关键词： Co.mo. b.an Cryo.reservatio. Cryo.to.age

根据重、磁和地质资料推断上莱茵地盾基底的主要构造特征

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地球物理学进展 1990年第2期5卷 45-45页

作者： J.b.edel.P.Fluck 林邦佐

根据重、磁资料的解释及其与露头和井孔地质观测资料关系推断,构制了一幅上莱茵地盾区前威斯特伐利亚阶基底的地质素描图。该图显示出“重”而“磁性”的Saxo.thuringian和“轻”的Mo.danub.an之间的接触关系,前者为有不同程度变质的古... 详细信息

根据重、磁资料的解释及其与露头和井孔地质观测资料关系推断,构制了一幅上莱茵地盾区前威斯特伐利亚阶基底的地质素描图。该图显示出“重”而“磁性”的Saxo.thuringian和“轻”的Mo.danub.an之间的接触关系,前者为有不同程度变质的古生界单元,并受到早石炭世基性岩的侵入,后者主要由片麻岩和花岗岩组成.这些地质结构和构造特征记录在中生代和新生代盖层之下.该墓底现在的图象主要反映晚韦宪阶和早纳尔阶(C1,欧洲)的构造特征,表现为一些磁性体及走滑断层和冲断层呈线性排列.其中主要的构造有作为Saxo.huringian和Mo.danub.an之17边的E-W向Vittel-Lub.ne-b.de-b.den断层、沿现代莱茵地堑延伸的N35。左旋平搓断层系、N 60。走向的北Vo.ges和Saar盆地,以及o.danub.anS形延伸的壳内逆冲断层,

关键词：构造特征地盾冲断层走滑断层磁性体盖层地堑地质素描线性排列地质资料

Effect o. Gastro.ntestinal Pro.ease Digestio. o. b.o.ctivity o. Marine Peptides

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Jo.rnal o. Agricultural Science and Techno.o.y(b. 2014年第1期4卷 74-84页

作者： Ida-Jo.anne Jensen Lisa Lystb.k Andersen Carlo.Gunnar o.sum Greta Jako.0}en Cristian De Go.0}a Sab.ena Farvin Ko.uvayur Hab.eb.llah Inez Jo.ansso. Else Ho.fmann edel.o.dny Elvevo.l Flemming Jessen Henrik Hauch Nielsen Faculty o. b.o.cience Fisheries and Eco.o.ics Norwegian College of FisheO Science UniversiO of Tromso Tromso 9037Norway Divisio. o.Industrial Fo.d Research National Food Institute DTU-Food Technical University qf Denmark Lyngby DK-2800Denmark Department o. b.o.o.y Cell and Developmental Biology Universitv of Copenhagen Copenhagen 2100 Denmark Marino.a ApS Adelvej 11 Lem St. 6940 Denmark Department o. Fo.d Science University o[Copenhagen Frederiksberg C 1958 Denmark

Fo.us in nutritio.al science has turned to.ards co.po.ents in, o. added to. fo.ds that may po.sess health b.neficial activities b.yo.d the classical nutritio.al value, namely functio.al fo.d. b.o.ctive peptides are examples o. such co.po.ents. In vitro.studies o. b.o.ctivities have mainly b.en executed witho.t co.cerning sub.equent digestio. after intake and the aim o. this wo.k was hence to.investigate ho. the in vitro.antio.idative, antihypertensive and caspase activating activities o. peptides are affected b. digestio. with gastro.ntestinal (GI) pro.eases. Five different fish pro.ein hydro.ysates were cho.en to.study the effect o. in vitro.digestio. o. b.o.ctivity. The pro.ein co.centratio. decreased in all samples during digestio. and the mo.ecular weight distrib.tio. o. the peptides shifted to.ards lo.er values. Thus, in vitro.digestio. with GI pro.eases resulted in a further degradatio. o. the peptides o.0}ained b. hydro.ysis. The antihypertensive effect increased in all samples after digestio. with GI pro.eases whereas the antio.idative capacity decreased. The effect o. the caspase activity depended o. the pro.eases used in the preparatio. o. hydro.ysates. In co.clusio., the caspase activity and antihypertensive activity are maintained during digestio. with GI pro.eases, while the antio.idative capacity seems to.b. reduced.

关键词：生物活性肽体外消化肠蛋白酶海洋肽胃肠道 caspase 蛋白水解物抗氧化能力