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Evaluation of occupational noise pollution and attitudes of exposed workers in two metal industries

Chinese Journal of Acoustics 2009年第4期28卷 289-297页

作者： Ghorbanali Mohammadi Faculty of Industrial Engineering Department College of EngineeringShahid Bahonar University of Kerman KermanIran 761889167

The purpose of the present study was to determined noise levels and to show the annoyance reaction, hearing loss, hearing impairment by the workers due to the occupational noise exposure according to Iran development of Environment （IDE） and also awareness about effects of noise and use of hearing protective devices （HPD） in the two manufacturing electronic equipment （metal） industries. The present study was carried out among industrial workers and included a sample of lao workers, site 1 （n = 52） and site 2 （n = 78） from two metal industries located in south eastern Iran province of Kerman. In order to attend the objectives of the current study, noise levels evaluation was determined throughout all different work stations in both industries. The results of the study revealed that noise levels in 75% of the stations are higher than limits used for assessment of noise for community response. The result of the study indicated that noise levels in the workplace are （66%） very high, （28%） high and only 6% low. Workers having higher education are much aware of the health impact due to occupational noise. Chi-square analysis indicated that the difference in educational background and not wearing HPD was significant and also a significant relationship was also observed between the impact of occupational noise on the health and education status. This research has clearly demonstrated that the workers in both the metal industries included in this study are at high risk of developing noise induced hearing loss and other associated ailments due to excessive occupational exposure to noise. There is a need to develop and apply a well-defined, comprehensive and enforceable training programs and noise regulation. The study revealed that the workers having higher education （9-12 grades） are much aware of the health impact due to occupational noise exposure. There is a need to establish a hearing conservation programs in both industries, the components of such program include noise

关键词： work In Evaluation of occupational noise pollution and attitudes of exposed workers in two metal industries

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Acylhydrazide and Isatin Schiff Bases as Alternate UV-Laser Desorption Ionization (LDI) Matrices for Low Molecular Weight (LMW) Peptides Analysis

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American Journal of Analytical Chemistry 2012年第12期3卷 779-789页

作者： Syed Ghulam Musharraf Aisha Bibi Najia shahid Muhammad Najam-ul-Haq Momin Khan Muhammad Taha Uzma Rasool Mughal Khalid Mohammed Khan Department of Chemistry Bahauddin Zakariya University Multan Pakistan Hussain Ebrahim Jamal Research Institute of Chemistry International Center for Chemical and Biological Sciences University of Karachi Karachi Pakistan Panjwani Center for Molecular Medicine and Drug Research International Center for Chemical and Biological Sciences University of Karachi Karachi Pakistan

Matrix-assisted laser desorption/ionization (MALDI) is a preferred and widely used mass spectrometric technique for the analysis of macromolecules. Limited UV-LDI matrices are available for the analysis of biomolecules due to the restricted structural features to serve in the laser desorption/ionization mechanism with a problem of background signals appearing in the low mass region. This paper describes the application of Schiff base derivatives of acylhydrazide and isatin as alternate UV-LDI matrices for the analysis of peptides with significantly low background signals. Thirty one compounds have been successfully employed as matrices for the analysis of low molecular weight (LMW) peptides (α-Cyano-4-hydroxycinnamic acid (HCCA), a preferred choice for peptide analysis.

关键词： MALDI-MS LDI Matrix Acylhydrazide Schiff Bases Bis-Schiff Bases of Isatin Schiff Bases of Isatin Peptides

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巴基斯坦卡拉奇─海得拉巴区域地震危险性预测

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地震研究 1995年第3期18卷 306-316页

作者： shahid A. Khan(MSSP, PINSTECH, P. O. Box 1114 Pakistan Atomic Energy Commission Islamabad,Pakistan)

本文讨论了巴基斯坦的地震构造，潜在震源和历史与现代地震活动性，并在此基础上使用确定性和概率性两种方法计算了各发震断层５０年内最大可能的地震在卡拉奇和海得拉巴两城市将引起的地面峰值加速度以及５０年内超越概率为１４％的等... 详细信息

本文讨论了巴基斯坦的地震构造，潜在震源和历史与现代地震活动性，并在此基础上使用确定性和概率性两种方法计算了各发震断层５０年内最大可能的地震在卡拉奇和海得拉巴两城市将引起的地面峰值加速度以及５０年内超越概率为１４％的等加速度区划图。

关键词：地震危险性分析地震区划巴基斯坦地震构造

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Morphogenetic Study of Pepino and Other Members of Solanaceae Family

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American Journal of Plant Sciences 2014年第26期5卷 3761-3768页

作者： Habib Ahmad Ayaz Khan Khushi Muhammad Muhammad shahid Nadeem Waqar Ahmad Sajjad Iqbal Aniqa Nosheen Nazia Akbar Israr Ahmad Youxiong Que Department of Genetics Hazara University Manshera Pakistan Institute of Chemical and Biological Sciences Department of Botany University of the Gujrat Gujrat Pakistan Key Lab of Sugarcane Biology and Genetic Breeding Ministry of Agriculture Fujian Agriculture and Forestry University Fuzhou China

This paper reports the findings regarding morphology, DNA markers and fruit quality of Solanum muricatum L., a newly introduced fruit in Hazara University, Pakistan, and other locally grown members of the Solanaceae family. In this study, RAPD (Random Amplified Polymorphic NA) based PCR report on phylogenetic affinities of pepino with brinjal, chilies, potato and tomato was also included. Total genomic DNA was isolated and 120 arbitrary decamers were applied to the genomic DNA of all these species. Only five out of the 120 decamers produced 27 polymorphic loci with the range of 400 - 1400 bp. We observed the specificity of markers, and primer B-08 amplified 700 bp locus linked to Solanum muricatum L., (Pepino), which could be used to identify this particular species of this family. Cluster analysis was also performed using the DNAMAN software (version 5.***.2.0) against the bivariate data collected from the products of several decamers. The UPGMA (Unweighted pair Group Method with Arithmetic Mean) analyses depicted three distinct groups, i.e. group I sorted out brinjal-tomato, group II sorted out chili-potato and pepino sorted out into its independent group III. Though on the basis of morphological traits pepino clustered with tomato, its DNA analyses proved it as a distinct species, far more related to the tomato. Moreover a total six parameters in biochemical analysis were studied, which revealed that pepino is a juicy fruit with maximum 93.25% moisture. This study could be helpful to maximize the new crop pepino in Pakistan.

关键词： Phylogeny Genetic Identity PCR Pepino RAPD

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Comparison of Efficacy of Synthetic Pesticides with Botanical Extracts under Field Condition on Cabbage White Butterfly (<i>Pieris brassicae</i>)

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Advances in Entomology 2021年第1期9卷 44-48页

作者： Aneela Kanwal Mehboob Ahmad Isma Khurshid Muhammad Pervaiz Khan Shitab Khan Imran Ullah Farkhanda Khan Muhammad shahid Shoaibullah Bashir Mah Rukh Hazara agricultural research station Abbottabad Pakistan Agricultural Research Institute D. I. Khan Pakistan Agricultural Research Station Chitral Pakistan Baacha Khan University Charsadda Pakistan

Cabbage white butterfly (Pieris brassicae) is one of the important insect pests of cabbage crop which causes remarkable quantitative or qualitative crop losses. The research was conducted at Hazara Agricultural research station, Abbottabad (Pakistan) to study the effect of different new chemical insecticides, botanical oil and neem seed oil (Azadirachta indica A. Juss.) on the population density of P. brassicae. The study showed that neem oil had a significant effect on population of P. brassicae in comparison to control treatment. So, neem oil alone or in combination with insecticides can be used for control of P. brassicae in vegetable crops for a safer food supply.

关键词： Botanical Extracts Efficacy Cabbage White Butterfly

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Science Missions Using CubeSats

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空间科学学报 2020年第4期40卷 443-461页

作者： SEYEDABADI M E FALANGA M azam M BARESI N FLÉRON R JANTARACHOTE V JUAREZ ORTIZ V A JULCA YAYA J J LANGER M MANUTHASNA S MARTINOD N MUGHAL M R NOMAN M PARK J PIMNOO A PRAKS J REYNERI L SANNA A ŞIŞMAN TÇ SOME J ULAMBAYAR T YU Xiaozhou DONG Xiaolong BALDIS L Asia-Pacific Space Cooperation Organization 100070 BeijingChina International Space Science Institute-Beijing 100190 BeijingChina International Space Science Institute 3012 BernSwitzerland Bangladesh Space Research and Remote Sensing Organization 1207 DhakaBangladesh Japan Aerospace Exploration Agency Institute of Space Astronautical Science252-5210 TokyoJapan Technical University of Denmark 2800 LyngbyDenmark Department of Electrical Engineering Faculty of EngineeringPrince of Songkla University90112 SongkhlaThailand National Commission of Aerospace Research and Development 15046 San IsidroPeru Technical University of Munich 80333 MunichGermany Mahanakorn University of Technology 10530 BangkokThailand Swiss Federal Institute of Technology Lausanne 1015 LausanneSwitzerland Institute of Space Technology 44000 IslamabadPakistan Korea Astronomy and Space Science Institute 34055 DaejeonSouth Korea Geo-Informatics and Space Technology Development Agency 10210 BangkokThailand Department of Electronics and Nanoengineering Aalto University02150 EspooFinland Department of Electronics and Telecommunications Polytechnic of Turin10129 TurinItaly Department of Physics University of Cagliari09042 MonserratoItaly Department of Astronautical Engineering University of Turkish Aeronautical Association06790 AnkaraTurkey Nanosatellite Development Laboratory National University of Mongolia210646 UlaanbaatarMongolia Dalian University of Technology 116024 DalianChina National Space Science Center 100190 BeijingChina

As the role of missions and experiments carried out in outer space becomes more and more essential in our understanding of many earthly problems,such as resource management,environmental problems,and disaster management,as well as space science questions,thanks to their lower cost and faster development process CubeSats can benefit humanity and therefore,young scientists and engineers have been motivated to research and develop new CubeSat *** very long after their inception,CubeSats have evolved to become accepted platforms for scientific and commercial *** last couple of years showed that they are a feasible tool for conducting scientific experiments,not only in the Earth orbit but also in the interplanetary *** many countries,a CubeSat mission could prompt the community and young teams around the world to build the national capacity to launch and operate national space *** paper presents an overview of the key scientific and engineering gateways opened up to the younger scientific community by the advent and adaptation of new technology into CubeSat *** role of cooperation and the opportunities for capacity-building and education are also ***,the present article also aims to provide useful recommendations to scientists,early-career researchers,engineers,students,and anyone who intends to explore the potential and opportunities offered by CubeSats and CubeSats-based missions.

关键词： CubeSat Space science Small satellites for space science Education Earth observation

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Comparative analysis of the complete chloroplast genome among Prunus mume,***,and ***

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Horticulture Research 2019年第1期6卷 626-638页

作者： Song Xue Ting Shi Wenjie Luo Xiaopeng Ni shahid Iqbal Zhaojun Ni Xiao Huang Dan Yao Zhijun Shen Zhihong Gao College of Horticulture Nanjing Agricultural University210095 NanjingChina Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement NanjingChina

Prunus mume *** Zucc.,*** L.,and *** *** economically important fruit trees in temperate *** species are taxonomically perplexing because of shared interspecific morphological traits and variation,which are mainly attributed to *** chloroplast is cytoplasmically inherited and often used for evolutionary *** sequenced the complete chloroplast genomes of ***,***,and *** using Illumina sequencing followed by de novo *** three chloroplast genomes exhibit a typical quadripartite structure with conserved genome arrangement,structure,and moderate *** lengths of the genomes are 157,815,157,797,and 157,916 bp,*** length of the large single-copy region(LSC)region is 86,113,86,283,and 86,122 bp,and the length of the SSC region is 18,916,18,734,and 19,028 bp;the IR region is 26,393,26,390,and 26,383 bp,*** of the three chloroplast genomes encodes 133 genes,including 94 protein-coding,31 tRNA,and eight rRNA *** gene analysis for the three species revealed that trnY-ATA is a unique gene in ***;in contrast,the gene trnI-TAT is only present in *** and ***,though the position of the gene in these chloroplast genomes *** comparative analysis of the complete chloroplast genome sequences revealed that the ORF genes and the sequences of linked regions rps16 and atpA,atpH and atpI,trnc-GCA and psbD,ycf3 and atpB,and rpL32 and ndhD are significantly different and may be used as molecular markers in taxonomic *** evolution analysis of the three species suggests that *** has a closer genetic relationship to *** than to ***.

关键词： rRNA traits analysis

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普通野生稻中S5n基因的鉴定及其胚囊育性研究

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科学通报 2010年第11期55卷 1007-1014页

作者：魏常敏王兰杨有新陈志雄 Muhammad Qasim shahid 李金泉刘向东卢永根华南农业大学广东省植物分子育种重点实验室广州510642

普通野生稻具有丰富的遗传多样性,蕴藏许多有利基因.利用S5n功能性标记对来自中国14个不同居群的441份普通野生稻进行检测,发现其中18份可能携带有S5n基因,全部为杂合型,分别来自5个居群,包括广东遂溪13份,广西玉林2份,海南临高、广东... 详细信息

普通野生稻具有丰富的遗传多样性,蕴藏许多有利基因.利用S5n功能性标记对来自中国14个不同居群的441份普通野生稻进行检测,发现其中18份可能携带有S5n基因,全部为杂合型,分别来自5个居群,包括广东遂溪13份,广西玉林2份,海南临高、广东高州和江西东乡各1份.进一步对这些材料S5座位可能存在的缺失及其两端DNA进行测序,发现全部材料缺失的DNA片段都与已知携带有S5n的品种02428一致,说明这18份材料确实存在S5n基因.对其中的15份材料自交一代进行基因型检测,检测到3种不同的基因型植株,其分离比(S5iS5i/S5jS5j:S5nS5i/j:S5nS5n)严重偏离1:2:1,其中S5n的杂合型和纯合型植株比例明显偏少,说明部分S5n配子可能无法正常受精.对4份代表性材料的S5座位进行全序列测定并与栽培稻比较,显示普通野生稻S5n序列出现少数碱基的差异.初步推测S5n基因在普通野生稻中已形成,属于古老的基因.对携带有S5n材料的胚囊育性进行研究,发现育性总体偏低,表明这些材料还可能存在除S5座位外决定胚囊育性其他座位的互作.

关键词：水稻(Oryza sativa L.) S5n 普通野生稻(O.rafipogon Griff.) 序列分析胚囊育性

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筛选和转化药用野生稻TAC克隆获得耐旱水稻

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中国农业科学 2014年第8期47卷 1445-1457页

作者：刘蕊张欢欢陈志雄 shahid Muhammad Qasim 傅雪琳刘耀光刘向东卢永根华南农业大学亚热带农业生物资源保护与利用国家重点实验室

【目的】利用分子生物学技术建立一种可有效转移药用野生稻有利基因的方法,为进一步大量开展利用药用野生稻有利基因提供技术基础。【方法】以植物抗性相关转录因子AP2/EREBP和bZIP家族基因保守序列为探针,采用同位素α-32P对探针进行标... 详细信息

【目的】利用分子生物学技术建立一种可有效转移药用野生稻有利基因的方法,为进一步大量开展利用药用野生稻有利基因提供技术基础。【方法】以植物抗性相关转录因子AP2/EREBP和bZIP家族基因保守序列为探针,采用同位素α-32P对探针进行标记,通过Southern杂交对药用野生稻TAC文库进行第一轮筛选;所获得阳性克隆用地高辛标记的探针进行第二轮筛选;最后利用PCR方法对第二轮筛选所获得的阳性克隆进行验证。通过农杆菌介导法将阳性克隆转入栽培稻品种日本晴,利用TAC载体插入片段两端的抗性标记Hpt和SacB,通过PCR扩增检测和和Southern杂交对转化植株进行鉴定。采用PEG胁迫法和生理指标变化对转基因T2植株的芽期、苗期耐旱性进行鉴定。【结果】第一轮TAC文库筛选共获得1 073个阳性克隆,其中,以AP2/EREBP的保守序列为探针筛选出606个、bZIP为探针筛选出467个。对这些阳性克隆进行第二轮筛选,从中共获得147个阳性克隆,其中,AP2/EREBP为探针筛选出95个、bZIP为探针筛选出52个。PCR验证有103个阳性克隆能够扩增出目的片段,分别是利用AP2/EREBP设计的引物筛选出63个克隆和bZIP的40个克隆,阳性克隆得率分别为66.32%和76.92%。通过农杆菌转化,以编号为52D-M16和22D-P2(与AP2/EREBP相关)以及55R-A17、49R-O14和8-A24(与bZIP相关)的克隆转化水稻日本晴均获得转化植株,转化试验结果发现外源插入片段越大则克隆转化的成苗率越低。抗性鉴定、PCR和Southern杂交结果均证明外源片段已转入受体水稻品种日本晴中。芽期抗旱性鉴定表明R12-23、R15-41和R1-8 3份材料的耐旱性优于受体日本晴,苗期鉴定显示M63-9和R12-23的耐旱性较强。综合认为,R12-23具有较好的耐旱性。【结论】通过构建药用野生稻TAC文库并结合特异克隆筛选和遗传转化方法,可以进行有利基因的转移,结合进一步的育种,有望实现药用野生稻在育种上的利用。

关键词：药用野生稻(Oryza officinalis Wall.) TAC文库遗传转化耐旱性

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从携带S_5~n基因的水稻种质中发掘S_a^n、S_b^n和S_c^n基因

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作物学报 2013年第8期39卷 1366-1376页

作者：李宏岩王思哲 shahid Muhammad Qasim 陈志雄王兰陈凤宜刘向东卢永根华南农业大学/亚热带农业生物资源保护与利用国家重点实验室广东广州510642

花粉不育是籼粳杂种F1优势利用的主要障碍之一。包括Sa、Sb和Sc等至少6个基因座位内的等位基因互作会引起花粉不育,这些座位上的中性等位基因可以克服不育性。本文用携带S5n的水稻种质,分别与台中65及其携带花粉不育基因的一套近等基因... 详细信息

花粉不育是籼粳杂种F1优势利用的主要障碍之一。包括Sa、Sb和Sc等至少6个基因座位内的等位基因互作会引起花粉不育,这些座位上的中性等位基因可以克服不育性。本文用携带S5n的水稻种质,分别与台中65及其携带花粉不育基因的一套近等基因系杂交,组配具有单个座位互作和多个座位同时互作的杂种F1,首先通过观察杂种F1的花粉育性并比较相应杂种F1育性的差异,初步判断是否具有中性等位基因,然后采用与Sa、Sb和Sc座位紧密连锁的分子标记对F2植株基因型的分离进行检测,并分析其分离比例的符合度,确定存在中性等位基因的真实性。结果发现在所鉴定的6份材料中有2份(灰背子和Madhukar)同时携带San和Sbn,3份(饭毫皮、秕五升和粤泰B)携带Sbn,1份(Jackson)携带Scn。这些材料同时携带可克服杂种F1胚囊不育和花粉不育的基因,是克服籼粳杂种F1不育性的重要基因来源。

关键词：水稻(OryzasativaL ) 等位基因互作杂种不育性雄性不育 S5n

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