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Influence of Agitation Rate on the Growth of MD2 Pineapple Protocorm-Like Bodies and Shoots in Liquid-Shake Culture

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American Journal of Plant Sciences 2019年第7期10卷 1233-1238页

作者： Zuraida Ab Rahman Hartinee Abbas ayu nazreena othman Wan Zaliha Wan Sembok Biotechnology and Nanotechnology Research Centre Malaysian Agricultural Research and Development Institute (MARDI) Kuala Lumpur Malaysia Horticulture Research Centre Malaysian Agricultural Research and Development Institute (MARDI) Sintok Malaysia School of Food Science and Technology Universiti Malaysia Terengganu Kuala Terengganu Malaysia

The present study was conducted to investigate the effect of agitation rate on the increase in fresh weight of MD2 pineapple protocorm-like bodies (PLBs) and shoots cultured in liquid medium. PLBs were cultured in 250 ml Erlenmeyer flasks (7 g per flask) containing MS medium and plant growth regulators (1.5 mg/L 6-Benzylaminopurine, BAP and 0.2 mg/L 1-Naphthaleneacetic acid, NAA). The orbital shaker was set at speeds of 50, 80, 100, 120, and 150 rpm. After 40 days, the cultures shaken at 80 rpm showed the highest fresh weight and the highest number of shoots at 76 g and 41 shoots, respectively. A comparative study of agitation found that 80 rpm was the best speed which enhanced both PLB and shoot formation. The findings in the present study would be helpful in setting up large-scale in vitro mass propagation of MD2 pineapple.

关键词： Pineapple MD2 Micropropagation Liquid-Shake Culture Agitation Rate

Direct Shoot Regeneration from Callus of Melicope lunu-ankenda

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Natural Science 2015年第2期7卷 81-87页

作者： Ab. Zuraida Rahman ayu nazreena othman Fatin Liyana Izzati Kamaruddin Aziz Bin Ahmad Biotechnology Research Centre MARDI Headquarters Persiaran MARDI-UPM Serdang Malaysia School of Science and Food Technology Universiti Malaysia Terengganu Kuala Nerus Malaysia Centre for Fundamental and Liberal Education Universiti Malaysia Terengganu Kuala Nerus Malaysia

Melicope lunu-ankenda is commonly used in traditional medicine. The conventional propagation method for this species is inefficient due to low propagation rate and its lengthy period to maturity. In addition, insufficient planting materials often pose a problem for the plantation sector. The tissue culture technique is best alternative to overcome the problems. The callus induction and direct shoot regeneration protocols for M. lunu-ankenda were established. Callus was successfully initiated from leaves explants cultured in MS medium added with 2,4-D at concentrations 0.5 to 5.0 mg/L singly or in combination with NAA at concentrations 1.0 to 10 mg/L. Shoot was regenerated from callus in phytohormone-free medium, BAP at concentrations 0.5 - 5.0 mg/L singly or in combination of BAP with NAA or 2,4-D at concentration 0.5 and 1.0 mg/L, respectively. BAP at 1.0 mg/L induced the highest shoot regeneration rate (80%) and number of plantlet per calli. The established methods might be used for production of phytochemicals and plantlets in large scale.

关键词： Melicope lunu-ankenda Regeneration Plant Growth Regulator In Vitro

In VitroMicropropagation of a Valuable Medicinal Plant, Plectranthus amboinicus

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American Journal of Plant Sciences 2015年第8期6卷 1091-1097页

作者： Zuraida Ab Rahman Erny Sabrina Mohd Noor Mohd Shukri Mat Ali Razali Mirad ayu nazreena othman Biotechnology Research Centre MARDI Headquarters MARDI HQ Persiaran MARDI-UPM Serdang Malaysia Strategic Resource Centre MARDI HQ Persiaran MARDI-UPM Serdang Malaysia

The effect of the plant growth regulators benzyl amino purine (BAP), 1-naphthaleneacetic acid (NAA) and kinetin (KIN) on in vitro shoot induction and proliferation of Plectranthus amboinicus was examined. Explants obtained from lateral shoots and apical shoots of P. amboinicus were inoculated on Murashige and Skoog (MS) culture medium supplemented with different concentrations of BAP, NAA and KIN. When the effect of each growth regulator was considered singly, the highest rate of shoot induction (80% of explants producing shoots) and highest number of shoots produced (2.4 shoots per explant) were obtained from lateral shoot explants cultured on MS media supplemented with 3.0 mg/L BAP within 6 - 7 weeks. Better results were obtained using MS medium supplemented with 1 mg/L BAP + 5 mg/L NAA. Shoot proliferation rose to 85%, while 5.7 shoots per explants were recorded. Among the different media tested for rooting, MS medium supplemented with 1.0 mg/L IBA was the most effective for root induction. The quality of the roots obtained was better than that obtained using MS media supplemented with NAA or IAA.

关键词： Plectranthus amboinicus Micropropagation Medicinal Plant Plant Growth Regulator

Callus Induction of Young Leaf Coconut cv. MATAG with Combination of 2,4-Dichlorophenoxyacetic Acid (2,4-D), α-Naphthalene Acetic Acid (NAA) and Benzyl Amino Purin (BAP)

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Advances in Bioscience and Biotechnology 2022年第5期13卷 254-263页

作者： Zuraida Ab Rahman Sentoor Kumaran Govindasamy Ahmad Ngalim Nur Auni Syazalien Adlan Nur Najwa Arifah Basiron ayu nazreena othman Biotechnology & Nanotechnology Research Centre Headquarters of Malaysian Agricultural Research and Development Institute Serdang Malaysia Crop Science and Soil Science Research Centre Headquarters of Malaysian Agricultural Research and Development Institute Serdang Malaysia Universiti Malaysia Pahang Gambang Campus Gambang Malaysia

This research was to study in vitro callus induction in Coconut cv MATAG from young leaf explants. Young leaf segments from mature coconut were cultured on Y3 medium supplemented with different concentrations of 2,4-D and a combination of NAA and BAP. Each of these plant growth regulators (PGR) gives different responses toward callus formation, the percentage of explants producing callus, the percentage of callus proliferation, and the morphology of callus. A series of different concentrations were used for 2,4-D (1, 5, 10, 20, 40, 60, 100 mg/L), NAA (1, 3, 5 mg/L) and BAP (1, 3, 5 mg/L) respectively. The range of days of callus formation using 2,4-D treatments is 7 - 12 months, while the 2,4-D combined with NAA is recorded at 2 - 5 months. Despite the variety of different months between these plant growth regulators for callus formation, the percentages of explants producing callus and callus proliferation are different. The highest percentage of explants producing callus (2.9%) was observed at 2,4-D (40 mg/mL), followed by 2.7% at 2,4-D (10.0 mg/mL) with NAA (1 mg/mL). At a concentration of 100 mg/mL of 2,4-D, the highest percentage of callus proliferation was found, as well.

关键词： Callus Induction Coconut NAA 2 4-D

Optimizing Extraction of Phenolics and Flavonoids from Solanum feroxFruit

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Natural Science 2019年第4期11卷 99-105页

作者： Zuraida Ab Rahman Mohd Waznul Adly Mohd Zaidan ayu nazreena othman Muhamad Aizuddin Ahmad Sanimah Simoh Muhammad Aiman Haiqal Ismail Biotechnology & Nanotechnology Research Centre MARDI HQ Serdang Malaysia

Various phenolic and flavonoid compounds that are secondary plant metabolites are known to contribute to physiological wellbeing. Extraction efficiency of such compounds from plant sources is dependent on the extraction solvent type and composition, and its pH. In this study, different extraction variables were examined: heating time (20 to 180 min), temperature (60°C, 75°C and 90°C) and pH (2.5, 3.0, 4.0, 5.0, 6.0 and 7.0). Hot water was used in the extraction of dry samples. For phenolics, the most efficient extraction was by using water at 60°C for 180 min, whereby 5.95 mg GA equivalent/dry extract was achieved. The most efficient extraction of flavonoids was achieved with water at 60°C for 150 min, whereby 43 μg Quercetin equivalent/dry extract was obtained. Adjusting the solvent to pH 2.5 increased the yield to 45.3 μg Quercetin equivalent/dry extract.

关键词： Phenolics Flavonoid Solanum ferox

Optimization of Extraction Conditions for Total Phenolics and Total Flavonoids from Kaempferia parvifloraRhizomes

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Advances in Bioscience and Biotechnology 2018年第5期9卷 205-214页

作者： Zuraida Ab Rahman Shazwan Abd Shukor Hartinee Abbas Chandradevan A. L. Machap Mohd Suhaimi Bin Alias Razali Mirad Syairah Sofiyanand ayu nazreena othman Biotechnology & amp Nanotechnology Research Centre MARDI HQ Persiaran MARDI-UPM Serdang Malaysia Horticulture Research Centre MARDI Sintok Bukit Kayu Hitam Malaysia Food Technology Research Centre MARDI HQ Persiaran MARDI-UPM Serdang Malaysia Agrobiodiversity and Environment Research Centre MARDI HQ Persiaran MARDI-UPM Serdang Malaysia Nilai Polytechnic Kompleks Pendidikan Bandar Enstek Bandar Enstek Malaysia

Kaempferia parviflora plants derived from in vitro culture were grown in the glasshouse. A comparison of the yield of total phenolics and total flavonoids under varying extraction conditions from rhizomes harvested from plants of different ages was undertaken. The results showed that phenolic and flavonoid contents in the rhizomes were highest 8 months after planting. Another study found that 2 g rhizomes extracted in 50 ml of water at 90°C for 120 minutes gave the best yield of phenolics and flavonoids. Under these conditions, an average of 210 mg GAE/g dry weight of total phenolics and 81 μg QCE/g dry weight of total flavonoids were obtained.

关键词： Phenolics Flavonoids Kaempferia parviflora Extraction

Establishment and Optimization Growth of Shoot Buds-Derived Callus and Suspension Cell Cultures of Kaempferia parviflora

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American Journal of Plant Sciences 2014年第18期5卷 2693-2699页

作者： Ab Rahman Zuraida othman ayu nazreena Kamarulzaman Fatin Liyana Izzati Ahmad Aziz Biotechnology Research Centre MARDI Headquarters MARDI HQ Persiaran MARDI-UPM Serdang Malaysia School of Science and Food Technology Kuala Terengganu Malaysia

Callus and suspension cells culture of Kaempferia parviflora was successfully established. Meristematic shoots can be used for utilization of plant cell biosynthetic capabilities for obtaining useful products from valuable medicinal plant to meet out the pharmaceutical demand and also for studying the metabolism. The medium containing combination of 0.2 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.2 mg/L napthyleneacetic acid (NAA) promoted the highest callus induction at 20%. Transferring the initiated callus on the medium with 1 mg/L 2,4-D enhanced the proliferation rate up to maximum fresh weight of 6.71 gm. Growth curve of cultured cells revealed that the cells continued to grow until 50 days of culture and showed the highest peak (fresh weight) at 40 days in all different initial weight tested ( 0.2, 0.5 and 1.0 gram). Isolated embryogenic callus was found to produce the highest in weight when suspended in liquid medium supplemented with 1 mg/L 2,4-D at 110 rpm resulted 13.5 gram fresh weight and 1080 mg dry weight.

关键词： Kaempferia parviflora Suspension Cultures Meristematic Shoots Callus Induction

In VitroRegeneration of Curcuma caesiaPlantlets from Basal Part and via Somatic Embryogenesis

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Advances in Bioscience and Biotechnology 2014年第4期5卷 363-372页

作者： Ab. Rahman Zuraida Kamaruddin Fatin Liyana Izzati othman ayu nazreena Che Mohd Zain Che Radziah Sheikh Ghadzi Siti Nur Asyikin Subramaniam Sreeramanan Biotechnology Research Centre MARDI Headquarters Persiaran MARDI-UPM Serdang Malaysia Faculty of Science and Technology Universiti Kebang-saan Malaysia Bangi Malaysia School of Biological Sciences Universiti Sains Malaysia (USM) Minden Heights Malaysia

Plantlets of Curcuma caesia were produced in vitro from newly sprouting vegetative buds of tubers. Segments of the plantlets from the junction between the root and the basal portion of the stem were subsequently used as explants to investigate factors affecting callus induction and plant regeneration via somatic embryogenesis. The explants were placed on Woody Plant Medium (WPM) together with different concentrations of 2,4-dichlorophenoxyacetic acid(2,4-D) and benzyl aminopurine (BAP) in the presence of light. The growth medium supplemented with 5 mg/L BAP and 2 mg/L 2,4-D promoted callus induction after 70 days of culture. Sub-culturing on the same medium enhanced the production of friable callus. Culture media containing higher concentrations of agar promoted the development of green somatic embryos from the callus. Respond of somatic embryogenesis was most successful with MS medium in 6.0 g/L agar supplemented with 5 mg/L BAP and 0.2 mg/L 2,4-D whereby the callus developed into green somatic embryos with an efficiency of 53%. This culture medium also produced the largest number plantlets.

关键词： In Vitro Regeneration Somatic Embryogenesis Curcuma caesia

A Simple and Efficient Protocol for the Mass Propagation of Vanilla planifolia

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American Journal of Plant Sciences 2013年第9期4卷 1685-1692页

作者： Ab Rahman Zuraida Kamarulzaman Hassan Fatin Liyana Izzati othman ayu nazreena Wan Sembok Wan Zaliha Che Mohd Zain Che Radziah Zainal Zamri Subramaniam Sreeramanan Biotechnology Research Centre MARDI Headquarters MARDI HQ Persiaran MARDI-UPM Serdang Selangor Malaysia Department of Agrotechnology Faculty of Agrotechnology and Food Science Universiti Malaysia Terengganu Kuala Terengganu Malaysia Faculty of Science and Technology Universiti Kebangsaan Malaysia Bangi Malaysia School of Biological Sciences Universiti Sains Malaysia (USM) Minden Heights Malaysia.

The present study describes a direct shoot regeneration-based micropropagation procedure for Vanilla planifolia. Two types of explant (i.e. shoot apex and stem nodal segment) were screened for their shoot induction potential following a three-month treatment with 6-benzylaminopurine (BAP) and α-Naphthaleneacetic acid (NAA). Results indicated that the shoot apices were poor candidates for shoot induction whereas the stem nodal segments showed potential for shoot initiation at a rate of up to 6 shoots/explant. Stem nodal segments were the most responsive as shoots formed (55 shoots) directly following treatments with 1 mg/L BAP at half strength MS medium after the third subculture. In addition, more shoots were produced on solid medium treatments compared to the liquid medium treatments in two strengths of mediums tested. Regenerated plantlets derived from the 1 mg/L of BAP treatment were induced to root following a one month culture in growth regulator-free MS medium. There was 90% survival rate of the rooted plantlets after acclimatization in the greenhouse. The findings in the present study would be helpful for large-scale mass propagation of Vanilla planifolia using this simple and efficient protocol.

关键词： Vanilla planifolia Mass Propagation Growth Regulator In Vitro BAP NAA IBA